• Animal Bioscience
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• 제35권7호
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• pp.1059-1068
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• 2022

Objective: This study was conducted to evaluate the effect of using low energy diet with multi-enzymes supplementation on different biological parameters in broilers. Methods: Three hundred Arbor Acres broiler chicks were randomly divided into three groups (Cont, standard metabolizable energy(ME); L-ME, ME reduced by 50 kcal/kg without enzyme; and L-ME-MES, L-ME diet was supplemented with multi-enzymes) with five replicates per group (20 chicks per replicate) at the start of second week. Grower and finisher diets were formulated according to breed specific guide and offered with free access in respective phase (two weeks for grower [8 to 21 d]; two weeks for finisher [22 to 35 d]). External marker method was used to measure the nutrient digestibility. After feeding trial, fifteen birds (one bird per replicate) were selected randomly and slaughtered for samples collection. Results: The results exhibited no effect (p>0.05) of dietary treatments on all parameters of growth performance, carcass traits, relative weight of internal organs except bursa and overall parameters of thigh meat quality. Relative weight of bursa was significantly (p<0.05) higher in L-ME than control. Multi-enzymes supplementation in low-ME diet significantly (p<0.05) improved the breast meat pH 24 h, digestibility of crude protein, duodenum weight and length, jejunal morphology, counts of Lactobacillus spp. and Bifidobacterium spp., lipase and protease activities than control. Jejunum length was increased in both L-ME and L-ME-MES treatments than that of the control (p<0.05). Breast meat cooking loss and color lightness was lower in L-ME (p<0.05) than control. Conclusion: It can therefore be concluded that broilers could be reared on low energy diet with supplementation of multi-enzymes without compromising the growth performance. In addition, it is beneficial for other biological parameters of broilers.

• Animal Bioscience
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• 제35권11호
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• pp.1760-1770
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• 2022

Objective: It is crucial to accurately determine the net energy (NE) values of feed ingredients because the NE system is expected to be applied to the formulation of broilers feed. The NE values of 5 wheat and 5 wheat brans were determined in 12-to 14-day old Arbor Acres (AA) broilers with substitution method and indirect calorimetry method. Methods: A total of 12 diets, including 2 reference diets (REF) and 10 test diets (5 wheat diets and 5 wheat bran diets) containing 30% of test ingredients, were randomly fed to 864 male AA birds with 6 replicates of 12 birds per treatment. These birds were used to determine metabolizable energy (ME) (8 birds per replicate) in the chicken house and NE (4 birds per replicate) in the chamber respectively at the same time. After a 4-d dietary and environment adaptation period, growth performance, energy values, energy balance and energy utilization were measured during the following 3 d. Multiple linear regression analyses were further performed to generate prediction equations for NE values based on the chemical components and ME values. The NE prediction equation were also validated on another wheat diet and another wheat bran diet with high correlation (r = 0.98, r = 0.75). Results: The NE values of 5 wheat and 5 wheat bran samples are 9.34, 10.02, 10.27, 11.33, and 10.49 MJ/kg, and 5.37, 5.17, 4.87, 5.06, and 4.88 MJ/kg DM, respectively. The equation with the best fit were NE = 1.968AME-0.411×ADF-14.227 (for wheat) and NE = -0.382×CF-0.362×CP-0.244×ADF+20.870 (for wheat bran). Conclusion: The mean NE values of wheat and wheat bran are 10.29 and 5.07 MJ/kg DM in AA broilers. The NE values of ingredients could be predicted by their chemical composition and energy value with good fitness.

• 한국가금학회지
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• 제49권4호
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• pp.247-253
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• 2022

본 연구는 우리나라 여름철에 육용 종계에게 γ-Amino-butyric(GABA)와 생균제를 급여하였을 때, 계란품질, 혈액 생화학 조성 및 corticosteron에 미치는 영향에 대하여 조사하였다. 본 연구는 49주령의 육용 종계 총 72수를 무작위로 선발하여 3 처리(대조구, GABA, 생균제)를 두었고, 시험구당 8반복으로 반복당 3수씩 완전임의배치하여 실험을 실시하였다. GABA와 생균제는 배합사료 내 각각 50 mg/kg과 0.1%의 농도로 급여하여 6주간 실험을 진행하였다. 6주 후 육용 종계의 난중, 난백고 높이, Haugh units, 난황색, 난각 두께 및 난각 강도에서 처리구간의 유의적인 차이를 보이지 않았다. 또한 모든 혈액 내 생화학 조성에서 GABA와 생균제 급여로 인한 유의적인 차이를 보이지 않았다. 혈중 corticosterone 농도는 CON의 14.18 ng/mL에비하여 GABA는 8.02 ng/mL 그리고 생균제는 8.30 ng/mL으로 크게 감소하였다(P<0.05). 이와 같은 corticosterone 수준의 감소는 스트레스 완화와 상호작용적 반응을 일으킨 것이라 사료된다. 결과적으로 본 연구에서 여름철 고온으로 인한 육용 종계 스트레스는 GABA 및 생균제 급여로 인하여 감소하였으며, 향후 이에 대한 보다 더 다양한 세부적 연구가 필요할 것으로 판단된다.

• Animal Bioscience
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• 제35권12호
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• pp.1921-1928
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• 2022

Objective: This research aimed to evaluate the effects of age on growth, tibia development, and intestinal calcium (Ca) and phosphorus (P) transporter gene expressions in broiler chickens. Methods: A total of 224 male Arbor Acres broilers were fed with nutrient-adequate diets and reared in eight cages (28 broilers per cage). Eight broilers (one broiler per cage) were selected and killed at 5, 10, 15, 20, 25, 30, 35, and 40 days of age, respectively. Results: Body weight continuously increased with age of broiler chickens from 5 to 40 days. The bone weight, ash weight, diameter, and length of the tibia also increased with broiler age. By contrast, the tibia ash, Ca, and P percentages quadratically changed with age (p<0.001), and the highest values of mineral contents were observed at 20, 25, and 25 days of age, respectively. The mRNA abundances of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), and plasma membrane ATPase 1b (PMCA1b) increased from 5 to 25 days and then decreased up to 40 days. Similar results were noted in the mRNA abundances of IIb sodium-phosphate cotransporter (NaPi-IIb), inorganic phosphate transporter 1 (PiT-1), inorganic phosphate transporter 2 (PiT-2), nuclear vitamin D receptor (nVDR), and membrane vitamin D receptor (mVDR). The mRNA abundances of Ca and P transporters and VDRs were the highest at 25 days of age. Conclusion: These data indicate that age quadratically affects intestinal Ca and P transporter gene expression and mineral absorption capacity in broiler chickens.

• Journal of Animal Science and Technology
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• 제65권6호
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• pp.1290-1307
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• 2023

This study investigated the effect of processed forms (defatted or hydrolyzed) of black soldier fly larvae (Hermetia illucens L., BSFL) as a protein substitute on broilers. Experiment 1 was a feeding experiment, and Experiment 2 was a metabolism experiment. In Experiment 1, a total of 120 day-old Arbor Acres broilers (initial body weight 39.52 ± 0.24 g) were used for 28 days. There were 8 replicate pens, and 5 broilers were assigned to each pen. In Experiment 2, a total of 36 day-old broilers (initial body weight 39.49 ± 0.21 g) were used for the metabolism trial. There were 2 broilers in a metabolism cage and six replicate cages per treatment. The dietary treatments were as follows: a basal diet (CON), a basal diet without fishmeal and substitute with defatted BSFL (T1), a basal diet without fishmeal and a substitute with hydrolyzed BSFL (T2). In Experiment 1, during the entire experimental period, the T2 group significantly increased (p < 0.05) body weight gain and feed intake compared to the CON and T1 groups. The feed conversion ratio showed a lower tendency (p = 0.057) in the T2 group than in the CON and T1 groups. At 2 weeks, the CON and T2 groups were significantly higher (p < 0.05) crude protein (CP) digestibility than the T1 group. At 4 weeks, the total protein level significantly increased (p < 0.05) in the CON and T2 groups compared to the T1 group. In Experiment 2, the CP digestibility significantly increased (p < 0.05) in the T2 group compared to the CON and T1 group at weeks 2 and 4. At week 4 amino acid digestibility, the T2 group significantly increased (p < 0.05) lysine, methionine, tryptophan, and glycine digestibility compared to the T1 group. There was no difference in fecal microbiota among the treatment groups. In conclusion, feeding hydrolyzed BSFL as a fishmeal substitute in broiler diets improved growth performance, CP digestibility, and specific amino acid digestibility. Therefore, it is considered that hydrolyzed BSFL in broiler diets can be sufficiently used as a new protein source.

• Journal of Animal Science and Technology
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• 제65권6호
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• pp.1226-1241
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• 2023

Selenium (Se) is an essential trace mineral that plays an important role in physiological processes by regulating the antioxidant defense system and enhancing immunity. Chromium is an essential mineral involved in carbohydrate and lipid metabolism and also plays a role in maintaining normal insulin function. Based on these advantages, we hypothesized that the addition of selenomethionine (SeMet) and organic chromium (OC) to broiler diets would increase Se deposition, antioxidant capacity and immune response in meat. Therefore, this study analyzed the effects of OC and SeMet on growh performance, nutrients digestibility, blood profiles, intestinal morphology, meat quality characteristics, and taxonomic analysis of broilers. A total of 168 one-day-old broiler chicken (Arbor Acres) were randomly allotted to 3 groups based on the initial body weight of 37.33 ± 0.24 g with 7 replicate per 8 birds (mixed sex). The experiments period was 28 days. Dietary treatments were folloewd: Basal diets based on corn-soybean meal (CON), basal diet supplemented with 0.2 ppm OC and 0.2 ppm SeMet (CS4), and basal diet supplemented with 0.4 ppm OC and 0.4 ppm SeMet (CS8). Supplementation of OC and SeMet did not affect on growth performance, nutrient digestibility. However, CS8 supplementation increased in duodenum villus height and villus height : crypt depth, and increased in breast meat Se deposition. In addition, CS8 group showed higher uric acid and total antioxidant status than CON group. Taxonomic analysis at phylum level revealed that Proteobacteria and Firmicutes of CS4 and CS8 were lower than CON group. In genus level, the relative abundance of fecal Lactobacillus and Enterococcus of CS4 and CS8 groups were higher than CON group. In short, 0.4 ppm OC and 0.4 ppm SeMet supplementation to broiler diet supporitng positive gut microbiome change, also enhancing antioxidant capacity, and Se deposition in breast meat.

• Animal Bioscience
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• 제37권7호
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• pp.1236-1245
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• 2024

Objective: The object of this study was to investigate the effect of replacing soybean meal with Clostridium autoethanogenum protein (CAP) in broiler diets on growth performance, blood indicators, antioxidant capacity, and immune function. Methods: A total of 180 Arbor Acres broilers were randomly divided into three treatments, each treatment with six replicates and 10 broilers per replicate for a 42-day feeding trial. The control group (CON) was fed corn-soybean meal based diet. The CAP-1 and CAP-2 groups were considered to use CAP to replace 25% or 50% of soybean meal in the diet, respectively. The average daily gain and average daily feed intake of broilers at 1 to 21 d, 22 to 42 d, and 1 to 42 d were measured, and the feed conversion ratio was calculated. At the 42nd day of age, two broilers with similar weights and fasted for 12 h were selected in each replicate for blood collection from the brachial wing vein. The blood routine indicators, serum biochemical indicators, serum antioxidant capacity, and immunoglobulin content of broiler chickens were measured. Results: Replacement of soybean meal with 25% (CAP-1) and 50% (CAP-2) CAP significantly increased the average daily gain of 22 to 42 d and 1 to 42 d and decreased the average daily feed intake and feed conversion rate (p<0.05). The CAP-1 group, and CAP-2 group significantly increased hemoglobulin in the blood of broilers, while the CAP-2 group increased hematocrit content (p<0.05). Compared with the control group, the contents of superoxide dismutase and immunoglobulin A in serum of the CAP-2 group were significantly increased, while the contents of malondialdehyde in CAP group were significantly decreased (p<0.05). Conclusion: Replacing soybean meal with CAP led to significant improvements in the growth performance, antioxidant capacity, and immunoglobulin content of broilers.

• 한국가금학회지
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• 제34권3호
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• pp.217-222
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• 2007

본 연구는 육계 사료 내 조단백질 수준에 따른 갯벌 미생물 유래 단백질 분해효소제를 첨가하였을 때 생산성, 영양소 소화율 및 혈액 내 total protein과 BUN 함량에 미치는 영향을 알아보기 위해 실시하였다. 사양 시험은 2일령 Arbor Acres 육계(male) 480수를 공시하여 5주간 실시하였다. 시험 설계는 기초 사료 내 조단백질 함량과 효소제의 첨가 유무에 따라 high crude protein diet 처리구, high crude protein diet에 단백질 분해 효소제를 첨가한 처리구(high crude protein diet+0.1% protease), low crude protein diet 처리구 및 low crude protein diet에 단백질 분해 효소제를 첨가한 처리구(low crude protein diet+0.1% protease)로 4처리($2{\times}2$ factorial)를 하여 처리당 6반복, 반복당 20수씩 완전 임의 배치하였다. 전체 사양 시험 기간 동안, 증체량은 high crude protein diet 처리구들과 low crude protein diet에 단백질 분해 효소제를 첨가한 처리구가 low crude protein diet 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 사료 섭취량에서는 처리구간에 유의적인 차이를 보이지 않았다. 사료 요구율에서는 high crude protein diet 처리구들과 low crude protein diet에 단백질 분해 효소제를 첨가한 처리구가 low crude protein diet 처리구와 비교하여 유의적으로 낮게 나타났다(P<0.05). 또한, 건물 소화율은 high crude protein diet 처리구들과 low crude protein diet에 단백질 분해효소제를 첨가한 처리구가 low crude protein diet 처리구와 비교하여 유의적으로 높게 나타났고(P<0.05), 질소 소화율에서는 high crude protein diet에 단백질 분해 효소제를 첨가한 처리구가 low crude protein diet 처리구와 비교하여 유의적으로 증가하였다(P<0.05). 혈액 내 total protein 함량은 high crude protein diet 처리구가 다른 처리구들과 비교하여 유의적으로 증가하였고(P<0.05), BUN 함량은 처리구들 간에 유의적인 차이를 보이지 않았다. 결론적으로, 육계 사료 내 조단백질 수준에 따른 갯벌 미생물 유래 단백질 분해효소제의 첨가는 증체량, 사료 요구율, 영양소 소화율을 개선시켰고, 혈액 내 total protein 함량에 영향을 미쳤다.