Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Evaluation of Clostridium autoethanogenum protein as a new protein source for broiler chickens in replacement of soybean meal

  • Xing Chen (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences) ;
  • Aijuan Zheng (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences) ;
  • Ahmed Pirzado Shoaib (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences) ;
  • Zhimin Chen (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences) ;
  • Kai Qiu (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences) ;
  • Zedong Wang (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences) ;
  • Wenhuan Chang (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences) ;
  • Huiyi Cai (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences) ;
  • Guohua Liu (Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences)
  • Received : 2023.10.16
  • Accepted : 2024.01.17
  • Published : 2024.07.01
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Abstract

Objective: The object of this study was to investigate the effect of replacing soybean meal with Clostridium autoethanogenum protein (CAP) in broiler diets on growth performance, blood indicators, antioxidant capacity, and immune function. Methods: A total of 180 Arbor Acres broilers were randomly divided into three treatments, each treatment with six replicates and 10 broilers per replicate for a 42-day feeding trial. The control group (CON) was fed corn-soybean meal based diet. The CAP-1 and CAP-2 groups were considered to use CAP to replace 25% or 50% of soybean meal in the diet, respectively. The average daily gain and average daily feed intake of broilers at 1 to 21 d, 22 to 42 d, and 1 to 42 d were measured, and the feed conversion ratio was calculated. At the 42nd day of age, two broilers with similar weights and fasted for 12 h were selected in each replicate for blood collection from the brachial wing vein. The blood routine indicators, serum biochemical indicators, serum antioxidant capacity, and immunoglobulin content of broiler chickens were measured. Results: Replacement of soybean meal with 25% (CAP-1) and 50% (CAP-2) CAP significantly increased the average daily gain of 22 to 42 d and 1 to 42 d and decreased the average daily feed intake and feed conversion rate (p<0.05). The CAP-1 group, and CAP-2 group significantly increased hemoglobulin in the blood of broilers, while the CAP-2 group increased hematocrit content (p<0.05). Compared with the control group, the contents of superoxide dismutase and immunoglobulin A in serum of the CAP-2 group were significantly increased, while the contents of malondialdehyde in CAP group were significantly decreased (p<0.05). Conclusion: Replacing soybean meal with CAP led to significant improvements in the growth performance, antioxidant capacity, and immunoglobulin content of broilers.

Keywords

Acknowledgement

The authors are grateful for the support by Professor Yang Peilong and Meng Kun to design of the present study.

References

  1. An SH, Kong C. Influence of dietary crude protein on growth performance and apparent and standardized ileal digestibility of amino acids in corn-soybean meal-based diets fed to broilers. Poult Sci 2023;102:102505. https://doi.org/10.1016/j.psj.2023.102505 
  2. Qiu K, Wang XC, Wang J, et al. Comparison of amino acid digestibility of soybean meal, cottonseed meal, and low-gossypol cottonseed meal between broilers and laying hens. Anim Biosci 2023;36:619-28. https://doi.org/10.5713/ab.22.0073 
  3. Yaqoob MU, Yousaf M, Imran S, et al. Effect of partially replacing soybean meal with sunflower meal with supplementation of multienzymes on growth performance, carcass characteristics, meat quality, ileal digestibility, digestive enzyme activity and caecal microbiota in broilers. Anim Biosci 2022;35:1575-84. https://doi.org/10.5713/ab.21.0553 
  4. Jiang Q, Wu W, Wan Y, et al. Energy values evaluation and improvement of soybean meal in broiler chickens through supplemental mutienzyme. Poult Sci 2022;101:101978. https://doi.org/10.1016/j.psj.2022.101978 
  5. Schafer L, Grundmann SM, Maheshwari G, et al. Effect of replacement of soybean oil by Hermetia illucens fat on performance, digestibility, cecal microbiome, liver transcriptome and liver and plasma lipidomes of broilers. J Anim Sci Biotechnol 2023;14:20. https://doi.org/10.1186/s40104-023-00831-6 
  6. Karim A, Gerliani N, Aider M. Kluyveromyces marxianus: an emerging yeast cell factory for applications in food and biotechnology. Int J Food Microbiol 2020;333:108818. https://doi.org/10.1016/j.ijfoodmicro.2020.108818 
  7. Wu Y, Wang J, Jia M, et al. Clostridium autoethanogenum protein inclusion in the diet for broiler: enhancement of growth performance, lipid metabolism, and gut microbiota. Front Vet Sci 2022;9:1028792. https://doi.org/10.3389/fvets.2022.1028792 
  8. Norman ROJ, Millat T, Winzer K, Minton NP, Hodgman C. Progress towards platform chemical production using Clostridium autoethanogenum. Biochem Soc Trans 2018;46:523-35. https://doi.org/10.1042/BST20170259 
  9. Ma S, Liang X, Chen P, et al. A new single-cell protein from Clostridium autoethanogenum as a functional protein for largemouth bass (Micropterus salmoides). Anim Nutr 2022;10:99-110. https://doi.org/10.1016/j.aninu.2022.04.005 
  10. Wei HC, Yu HH, Chen XM, et al. Effects of soybean meal replaced by Clostridium autoethanogenum protein on growth performance, plasma biochemical indexes and hepatopancreas and intestinal histopathology of grass carp (Ctenopharyngodon idllus). Chin J Anim Nutr 2018;30:4190-201. 
  11. Humphreys CM, McLean S, Schatschneider S, et al. Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium. BMC Genomics 2015;16:1085. https://doi.org/10.1186/s12864-015-2287-5 
  12. Yao W, Yang P, Zhang X, et al. Effects of replacing dietary fish meal with Clostridium autoethanogenum protein on growth and flesh quality of Pacific white shrimp (Litopenaeus vannamei). Aquaculture 2022;549:737770. https://doi.org/10.1016/j.aquaculture.2021.737770 
  13. Chen X, Zhao M, Zheng A, et al. Evaluation of the application value of cottonseed protein concentrate as a feed protein source in broiler chickens. Animals (Basel). 2023;13:3706. https://doi.org/10.3390/ani13233706 
  14. National Research Council. Nutrient requirements of poultry. 9th rev ed. Washington, DC, USA: National Academy Press; 1994. 
  15. Chen J, Niu X, Li F, Li F, Guo L. replacing soybean meal with distillers dried grains with solubles plus rumen-protected lysine and methionine: effects on growth performance, nutrients digestion, rumen fermentation, and serum parameters in Hu sheep. Animals (Basel) 2021;11:2428. https://doi.org/10.3390/ani11082428 
  16. Erdaw MM, Perez-Maldonado RA, Iji PA. Supplementation of broiler diets with high levels of microbial protease and phytase enables partial replacement of commercial soybean meal with raw, full-fat soybean. J Anim Physiol Anim Nutr (Berl) 2018;102:755-68. https://doi.org/10.1111/jpn.12876 
  17. Lagos LV, Stein HH. Chemical composition and amino acid digestibility of soybean meal produced in the United States, China, Argentina, Brazil, or India. J Anim Sci 2017;95:1626-36. https://doi.org/10.2527/jas.2017.1440 
  18. Siegert W, Ganzer C, Kluth H, Rodehutscord M. Effect of amino acid deficiency on precaecal amino acid digestibility in broiler chickens. J Anim Physiol Anim Nutr (Berl) 2019;103:723-37. https://doi.org/10.1111/jpn.13066 
  19. He W, Li P, Wu G. Amino acid nutrition and metabolism in chickens. Adv Exp Med Biol 2021;1285:109-31. https://doi.org/10.1007/978-3-030-54462-1_7 
  20. Zhou J, Wang L, Yang G, Yang L, Zeng X, Qiao S. Pea starch increases the dry matter flow at the distal ileum and reduces the amino acids digestibility in ileal digesta collected after 4 hours postprandial of pigs fed low-protein diets. Anim Biosci 2022;35:1021-9. https://doi.org/10.5713/ab.21.0354 
  21. Jespersen JC, Richert S, Cesar de Paula Dorigam J, Oelschlager ML, Dilger RN. Effects of lysine biomass supplementation on growth performance and clinical indicators in broiler chickens. Poult Sci 2021;100:100971. https://doi.org/10.1016/j.psj.2020.12.068 
  22. Miao ZQ, Dong YY, Qin X, et al. Dietary supplementation of methionine mitigates oxidative stress in broilers under high stocking density. Poult Sci 2021;100:101231. https://doi.org/10.1016/j.psj.2021.101231 
  23. Kumar CB, Gloridoss RG, Singh KC, Prabhu TM, Suresh BN. Performance of broiler chickens fed low protein, limiting amino acid supplemented diets formulated either on total or standardized ileal digestible amino acid basis. Asian-Australas J Anim Sci 2016;29:1616-24. https://doi.org/10.5713/ajas.15.0648 
  24. Cappelaere L, Le Cour Grandmaison J, Martin N, Lambert W. Amino acid supplementation to reduce environmental impacts of broiler and pig production: a review. Front Vet Sci 2021;8:689259. https://doi.org/10.3389/fvets.2021.689259 
  25. Kidd MT, Maynard CW, Mullenix GJ. Progress of amino acid nutrition for diet protein reduction in poultry. J Anim Sci Biotechnol 2021;12:45. https://doi.org/10.1186/s40104-021-00568-0 
  26. Liu W, Liu GH, Liao RB, et al. Apparent metabolizable and net energy values of corn and soybean meal for broiler breeding cocks. Poult Sci 2017;96:135-43. https://doi.org/10.3382/ps/pew195 
  27. Morgan NK, Keerqin C, Wallace A, Wu SB, Choct M. Effect of arabinoxylo-oligosaccharides and arabinoxylans on net energy and nutrient utilization in broilers. Anim Nutr 2019;5:56-62. https://doi.org/10.1016/j.aninu.2018.05.001 
  28. Xie K, He X, Hou DX, Zhang B, Song Z. Evaluation of nitrogen-corrected apparent metabolizable energy and standardized ileal amino acid digestibility of different sources of rice and rice milling byproducts in broilers. Animals (Basel) 2021;11:1894. https://doi.org/10.3390/ani11071894 
  29. Yu C, Yang W, Jiang S, Wang T, Yang Z. Effects of star anise (Illicium verum Hook.f.) essential oil administration under three different dietary energy levels on growth performance, nutrient, and energy utilization in broilers. Anim Sci J 2021;92:e13496. https://doi.org/10.1111/asj.13496 
  30. Chen J, Wang H, Yuan H, et al. Effects of dietary Clostridium autoethanogenum protein on the growth, disease resistance, intestinal digestion, immunity and microbiota structure of Litopenaeus vannamei reared at different water salinities. Front Immunol 2022;13:1034994. https://doi.org/10.3389/fimmu.2022.1034994 
  31. Yang P, Li X, Yao W, Li M, Wang Y, Leng X. Dietary effect of Clostridium autoethanogenum protein on growth, intestinal histology and flesh lipid metabolism of Largemouth Bass (Micropterus salmoides) based on metabolomics. Metabolites 2022;12:1088. https://doi.org/10.3390/metabo12111088 
  32. Shang Y, Kumar S, Oakley B, Kim WK. Chicken gut microbiota: importance and detection technology. Front Vet Sci 2018;5:254. https://doi.org/10.3389/fvets.2018.00254 
  33. Hu Y, Wang L, Shao D, et al. Selectived and reshaped early dominant microbial community in the cecum with similar proportions and better homogenization and species diversity due to organic acids as AGP alternatives mediate their effects on broilers growth. Front Microbiol 2020;10:2948. https://doi.org/10.3389/fmicb.2019.02948 
  34. Zhang Q, Zhang S, Wu S, Madsen MH, Shi S. Supplementing the early diet of broilers with soy protein concentrate can improve intestinal development and enhance short-chain fatty acid-producing microbes and short-chain fatty acids, especially butyric acid. J Anim Sci Biotechnol 2022;13:97. https://doi.org/10.1186/s40104-022-00749-5 
  35. Akter N, Islam MS, Zaman S, Jahan I, Hossain MA. The impact of different levels of L-methionine (L-Met) on carcass yield traits, serum metabolites, tibial characters, and profitability of broilers fed conventional diet. J Adv Vet Anim Res 2020;7:253-9. https://doi.org/10.5455/javar.2020.g417 
  36. Attia YA, Al-Khalaifah H, Abd El-Hamid HS, Al-Harthi MA, Alyileili SR, El-Shafey AA. Antioxidant status, blood constituents and immune response of broiler chickens fed two types of diets with or without different concentrations of active yeast. Animals (Basel) 2022;12:453. https://doi.org/10.3390/ani12040453 
  37. Hassan MI, Khalifah AM, El Sabry MI, Mohamed AE, Hassan SS. Performance traits and selected blood constituents of broiler chicks as influenced by early access to feed post-hatch. Anim Biotechnol 2023;34:2855-62. https://doi.org/10.1080/10495398.2022.2124164 
  38. Rehman ZU, Meng C, Sun Y, et al. Oxidative stress in poultry: lessons from the viral infections. Oxid Med Cell Longev 2018;2018:5123147. https://doi.org/10.1155/2018/5123147 
  39. Zaboli G, Huang X, Feng X, Ahn DU. How can heat stress affect chicken meat quality? - a review. Poult Sci 2019;98:1551-6. https://doi.org/10.3382/ps/pey399 
  40. Zhang H, Yu X, Li Q, et al. Effects of rhamnolipids on growth performance, immune function, and cecal microflora in Linnan yellow broilers challenged with Lipopolysaccharides. Antibiotics (Basel) 2021;10:905. https://doi.org/10.3390/antibiotics10080905 
  41. Wang X, Wang C, Wang Z, et al. Antioxidant effect of taurine on chronic heat-stressed broilers. Adv Exp Med Biol 2022;1370:161-9. https://doi.org/10.1007/978-3-030-93337-1_16 
  42. Hu H, Bai X, Xu K, Zhang C, Chen L. Effect of phloretin on growth performance, serum biochemical parameters and antioxidant profile in heat-stressed broilers. Poult Sci 2021;100:101217. https://doi.org/10.1016/j.psj.2021.101217 
  43. Lauridsen C. From oxidative stress to inflammation: redox balance and immune system. Poult Sci 2019;98:4240-6. https://doi.org/10.3382/ps/pey407 
  44. Liu SJ, Wang J, He TF, Liu HS, Piao XS. Effects of natural capsicum extract on growth performance, nutrient utilization, antioxidant status, immune function, and meat quality in broilers. Poult Sci 2021;100:101301. https://doi.org/10.1016/j.psj.2021.101301 
  45. Bai K, Feng C, Jiang L, et al. Dietary effects of Bacillus subtilis fmbj on growth performance, small intestinal morphology, and its antioxidant capacity of broilers. Poult Sci 2018;97:2312-21. https://doi.org/10.3382/ps/pey116 
  46. Wang Y, Li L, Gou Z, et al. Effects of maternal and dietary vitamin A on growth performance, meat quality, antioxidant status, and immune function of offspring broilers. Poult Sci 2020;99:3930-40. https://doi.org/10.1016/j.psj.2020.03.044 
  47. Liu T, Zhou J, Li W, et al. Effects of sporoderm-broken spores of Ganoderma lucidum on growth performance, antioxidant function and immune response of broilers. Anim Nutr 2020;6:39-46. https://doi.org/10.1016/j.aninu.2019.11.005 
  48. Song ZH, Cheng K, Zheng XC, Ahmad H, Zhang LL, Wang T. Effects of dietary supplementation with enzymatically treated Artemisia annua on growth performance, intestinal morphology, digestive enzyme activities, immunity, and antioxidant capacity of heat-stressed broilers. Poult Sci 2018;97:430-7. https://doi.org/10.3382/ps/pex312 
  49. Iqbal Z, Kamran Z, Sultan JI, et al. Replacement effect of vitamin E with grape polyphenols on antioxidant status, immune, and organs histopathological responses in broilers from 1- to 35-d age. J Appl Poult Res 2015;24:127-34. https://doi.org/10.3382/japr/pfv009