• Title/Summary/Keyword: Aqueous

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Reduction of Microbial Load on Radish (Raphanus sativus L.) Seeds by Aqueous Chlorine Dioxide and Hot Water Treatments (이산화염소수 및 열수처리에 따른 무(Raphanus sativus L.) 새싹 종자의 미생물 제어 효과)

  • Park, Kee-Jai;Lim, Jeong-Ho;Kim, Ji-Hye;Jeong, Jin-Woong;Jo, Jin-Ho;Jeong, Seong-Woong
    • Food Science and Preservation
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    • v.14 no.5
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    • pp.487-491
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    • 2007
  • This study was conducted to investigate the effect of treatment with squeous chlorine dioxide and hot water on the germination of radish (Raphanus sativus L.) seeds, and reduction of microbial load on the seeds. Increases in treatment and the concentration of aqueous chlorine dioxide in water resulted in increasing reductions in the counts of total aerobic microbes. Seeds treated with aqueous chlorine dioxide (100 ppm/20min, 200ppm/20min) showed about a 10-fold decrease in microbial loads. Germination of seeds was not adversely affected by any treatment tested, although the germination rate of seeds in the group treated at $55^{\circ}C$ for 20 min was reduced by 10% compared to that of control. Combined treatment with hot water and aqueous chlorine dioxide yielded better out comes in both microbial reduction and seed germination rate than did single treatments. A combined treatment with 100 ppm aqueous chlorine dioxide and hot water($45^{\circ}C$ or $50^{\circ}C$) resulted in about a 100-fold decrease in microbial load whereas germination rate showed only a slight increase to $97.0{\sim}97.7%$. Total aerobic microbial counts in radish seeds were decreased by aqueous chlorine dioxide and hot water treatment in the order. aqueous $CIO_2$+ hot water > aqueous $CIO_2$ > chlorinated water > hot water > control.

Rheological Properties of Exopolysaccharide p-KG03 Produced by Marine Microalgae Gyrodinium impudicum strain KG03

  • Im, Jeong-Han;Kim, Seong-Jin;Park, Gyu-Jin;An, Se-Hun;Lee, Hyeon-Sang;Lee, Hong-Geum
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.611-614
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    • 2003
  • The rheological properties of exopolysaccharide, p-KG03, produced by marine microalgae Gyrodinium impudicum strain KG03 had been studied. The intrinsic viscosity of this p-KG03 was calculated to 65.22 and 50.75 $d{\ell}/g$ using Huggins and Kramer equations (xanthan gum 24.41 and 24.03). Aqueous dispersions at p-KG03 concentrations ranging from 0.1 to 1.0 % (w/w) showed marked shear-thinning properties as Power-Law behavior. In aqueous dispersions of p-KG03 1.0 %, consistency index (K) and flow behavior index (n) were 2,172 and 0.52. The apparent viscosity and the influence of shear rate on different conditions as p-KG03 concentrations, pH, NaCl, $CaCl_2$ and temperature in aqueous solutions were measured. And p-KG03 had mixed with aqueous solutions of xanthan gum and gellan gum, and invested the change of mixed aqueous solution behavior.

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Comprehensive Studies on the Free Energies of Solvation and Conformers of Glycine: A Theoretical Study

  • Kim, Chang-Kon;Park, Byung-Ho;Lee, Hai-Whang;Kim, Chan-Kyung
    • Bulletin of the Korean Chemical Society
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    • v.32 no.6
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    • pp.1985-1992
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    • 2011
  • The stable conformers of glycine and the inter-conversions between them were studied theoretically at various levels of theory, B3LYP, MP2, CCSD and CCSD(T), in the gas phase and in aqueous solution. In aqueous solution, the structures examined by use of the conductor-like polarizable continuum model (CPCM) with various cavity models, UA0, UAHF, UAKS, UFF, BONDI and PAULING, and by use of a discrete/continuum solvation model with eight water clusters. The Gibbs free energy differences between the neutral (NE) and zwitterionic conformers (ZW), ${\Delta}G_{Z-N}[=G_{ZW}-G_{NE}]$, in aqueous solution were well reproduced by using the BONDI and PAULING cavity models. However the ${\Delta}G_{Z-N}$ values were underestimated in other cavity models, although the ZW conformers existed as stable species in aqueous solution. In the studies of a discrete/continuum solvation model with eight water clusters, gas phase results are still insufficient to reproduce the experimental findings. However the ${\Delta}G_{Z-N}$ values calculated by use of CPCM method in aqueous solution agreed well with the experimental ones.

Combined Treatment with Low Concentrations of Aqueous and Gaseous Chlorine Dioxide Inactivates Escherichia coli O157:H7 and Salmonella Typhimurium Inoculated on Paprika

  • Kim, Hyun-Gyu;Song, Kyung Bin
    • Journal of Microbiology and Biotechnology
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    • v.27 no.3
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    • pp.492-499
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    • 2017
  • Combined treatment with gaseous and aqueous chlorine dioxide ($ClO_2$) was performed to improve the microbiological safety and quality of paprika. A single treatment of 50 ppmv $ClO_2$ gas for 30 min decreased the populations of Escherichia coli O157:H7 and Salmonella Typhimurium by 2.33 and 2.91 log CFU/g, respectively. In addition, a single treatment of aqueous $ClO_2$ (50 ppm) for 5 min decreased these populations by 1.86 and 1.37, respectively. The most dramatic effects were achieved by combined treatment of 50 ppm aqueous and gaseous $ClO_2$ for 30 min, which decreased populations of E. coli O157:H7 and S. Typhimurium by 4.11 and 3.61 log CFU/g, respectively. With regard to the qualities of paprika, no adverse effects were elicited by the combined treatment. Thus, combined treatment with aqueous and gaseous $ClO_2$ is a suitable approach that can be used to improve the microbial safety and quality of paprika.

The Removal of Hexavalent Chromium from Aqueous Solutions Using Modified Holly Sawdust: Equilibrium and Kinetics Studies

  • Siboni, M. Shirzad;Samarghandi, M.R.;Azizian, S.;Kim, W.G.;Lee, S.M.
    • Environmental Engineering Research
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    • v.16 no.2
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    • pp.55-60
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    • 2011
  • The removal of hexavalent chromium from aqueous solutions onto modified holly sawdust was studied at varying initial hexavalent chromium concentrations, adsorbent doses, pHs and contact times. The removal of hexavalent chromium from aqueous solutions increased with increasing adsorbent dosage and contact time. The percentage of hexavalent chromium removed from the aqueous solutions decreased with increasing hexavalent chromium concentration and pH of the solution. The kinetics of the adsorption of hexavalent chromium onto modified holly sawdust was analyzed using pseudo first-order and pseudo second-order models. The pseudo second-order model described the kinetics of adsorption of hexavalent chromium. The Langmuir and Freundlich isotherm models were used for modeling of the adsorption equilibrium data. The Langmuir isotherm model well described the equilibrium data for the removal of hexavalent chromium by modified holly sawdust. The obtained maximum adsorption capacity was 18.86 mg/g at pH 7. The results showed that modified holly sawdust can be used as a low cost adsorbent for the treatment of aqueous solutions containing chromium.

Reverse Micellar Extraction of Fungal Glucoamylase Produced in Solid-State Fermentation Culture

  • Paraj, Aliakbar;Khanahmadi, Morteza;Karimi, Keikhosro;Taherzadeh, Mohammad J.
    • Journal of Microbiology and Biotechnology
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    • v.24 no.12
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    • pp.1690-1698
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    • 2014
  • Partial purification of glucoamylase from solid-state fermentation culture was, firstly, investigated by reverse micellar extraction (RME). To avoid back extraction problems, the glucoamylase was kept in the original aqueous phase, while the other undesired proteins/enzymes were moved to the reverse micellar organic phase. The individual and interaction effects of main factors (i.e., pH and NaCl concentration in the aqueous phase, and concentration of sodium bis-2-ethyl-hexyl-sulfosuccinate (AOT) in the organic phase) were studied using response surface methodology. The optimum conditions for the maximum recovery of the enzyme were pH 2.75, 100 mM NaCl, and 200 mM AOT. Furthermore, the optimum organic to aqueous volume ratio ($V_{org}/V_{aq}$) and appropriate number of sequential extraction stages were 2 and 3, respectively. Finally, 60% of the undesired enzymes including proteases and xylanases were removed from the aqueous phase, while 140% of glucoamylase activity was recovered in the aqueous phase and the purification factor of glucoamylase was found to be 3.0-fold.

Kinetics and Equilibrium Isotherm Studies for the Aqueous Lithium Recovery by Various Type Ion Exchange Resins

  • Won, Yong Sun;You, Hae-na;Lee, Min-Gyu
    • Korean Journal of Materials Research
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    • v.26 no.9
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    • pp.498-503
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    • 2016
  • The characteristics of aqueous lithium recovery by ion exchange were studied using three commercial cation exchange resins: CMP28 (porous type strong acid exchange resin), SCR-B (gel type strong acid exchange resin) and WK60L (porous type weak acid exchange resin). CMP28 was the most effective material for aqueous lithium recovery; its performance was even enhanced by modifying the cation with $K^+$. A comparison to $Na^+$ and $H^+$ form resins demonstrated that the performance enhancement is reciprocally related to the electronegativity of the cation form. Further kinetic and equilibrium isotherm studies with the $K^+$ form CMP28 showed that aqueous lithium recovery by ion exchange was well fitted with the pseudo-second-order rate equation and the Langmuir isotherm. The maximum ion exchange capacity of aqueous lithium recovery was found to be 14.28 mg/g and the optimal pH was in the region of 4-10.

Dispersion of SmxCe1-xO2-2/x Nanoparticles which is Synthesized by Hydrothermal Process in Aqueous System (수열합성법으로 합성된 나노 SmxCe1-xO2-2/x 분말의 수계 분산)

  • Bae Dong-Sik;Kim Eun-Jung;Han Kyong-Sop
    • Korean Journal of Materials Research
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    • v.15 no.2
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    • pp.112-114
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    • 2005
  • Dispersion stability of the $Sm_xCe_{1-x}O_{2-2/x}$ nanoparticles, which was produced by hydrothermal process, was studied in aqueous suspension using ESA (Eletrokinetic Sonic Amplitude). The average particle size of the synthesized $Sm_xCe_{1-x}O_{2-2/x}$ at nanoparticles was about $5{\pm}2nm$. The dispersion and rheological behavior of the $Sm_xCe_{1-x}O_{2-2/x}$ nanoparticles aqueous suspension was investigated using $NH_4OH\;and\;HNO_3$ as a disperse agent. The colloidal stability of aqueous suspensions with $Sm_xCe_{1-x}O_{2-2/x}$ nanoparticles at different pH values has been investigated by means of zeta potential, average particle size, and the distribution of synthesized $Sm_xCe_{1-x}O_{2-2/x}$ nanoparticles. The isoelectric point of the $Sm_xCe_{1-x}O_{2-2/x}$ nanoparticles was at pH around 11 and the value of zeta potential was at its maximum near pH 6.5.

In vitro antifilarial potential of the leaves and stem extract of Pluchea lanceolata on the cattle filarial parasite Setaria cervi

  • Mishra, Vandna;Parveen, Nazneen;Khan, Nizam U.;Singhal, KC
    • Advances in Traditional Medicine
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    • v.5 no.2
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    • pp.110-116
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    • 2005
  • The effect of alcohol and aqueous extracts of the leaves and stem of Pluchea lanceolata on the spontaneous movements of both the whole worm and the nerve muscle preparation of Setaria cervi, and on the survival of microfilariae in vitro was studied. Alcohol and aqueous extracts of the leaves and stem of P. lanceolata caused the inhibition of spontaneous movements of the whole worm and the nerve muscle preparation of S. cervi, characterized by short lasting small increase in tone and amplitude of contractions followed by paralysis. The concentrations required to inhibit the movements of whole worm and nerve muscle preparations for alcohol extract were 200 and $25\;{\mu}g/ml$, and for aqueous extract were 250 and $100\;{\mu}g/ml$, respectively, suggesting a cuticular permeability barrier. Both the extracts (alcohol and aqueous) caused death of microfilariae in vitro, $LC_{50}$ and $LC_{90}$ being 12 and 18 ng/ml for alcohol extract and 25 and 40 ng/ml for aqueous extract, respectively.

An Investigation of Solubility of Aliquat 336 in Different Extracted Solutions

  • Xu, Jianying;Paimin, Rohani;Shen, Wei;Wang, Xungai
    • Fibers and Polymers
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    • v.4 no.1
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    • pp.27-31
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    • 2003
  • A major concern in solvent extraction process is the loss of extractant into the aqueous phase due to its slight solubility in the aqueous phase. Similarly, in membrane extraction processes, extractant loss through extractant leakage from the membrane into the aqueous phase is also a concern. Several published membrane extraction studies using Aliquat 336 ai the extractant, have expressed this concern, but none has studied extractant leakage quantitatively. It is the authors' opinion that the extractant leakage should be considered at a technical parameter of a membrane. In our laboratory active progress has been made in using Aliquat 336 ‘entangled’ into the polymer membranes to remove heavy metal ions from wastewater samples. In this work, we studied the loss of Aliquat 336 from the point of view of its solubility in aqueous solutions. The results showed that the solubilities or Aliquat 3,36 in an aqueous phase acidified with 2 M HCI it about 0.1 g/100 m/ of the solution. This figure provides a useful guideline for evaluating the leakage of the Aliquatoat 336 extractant from the membranes.