• 한국물환경학회지
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• 제18권4호
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• pp.395-400
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• 2002

Lead is one of the most toxic metal and is detectable in practically all phases of environment and in all biological system. Transport, industrial and domestic waste products are the main sources of this pollutant. Ingested lead is rapidly absorbed and widely distributed throughout the body, causing extensive tissue damage. In this study, we chose the freshwater decapods Maceobrachium nipponnese as a sensitive indicator organism for environmental pollution. In order to investigate the possibility in use of $Na^+/K^+ATPase$ activity as a biomarker of lead pollution, we tested the acute toxicity of lead to Maceobrachium nipponnese. The $LC_{50}(96hr)$ value for lead in Maceobrachium nipponnese was found to be $446{\mu}g/L$ with the 95% confidence limits. The lead exposure group at $LC_{50}$ showed a significant $Na^+/K^+ATPase$ inhibition, depending on the exposure time. Comparision of several concentrations of lead revealed that the $Na^+/K^+ATPase$ activity in Maceobrachium nipponnese was significantly decreased in a concentration dependent manner. These results suggest that $Na^+/K^+ATPase$ activity in Maceobrachium nipponnese may possibly be used as a biomarker of lead pollution in aquatic ecosystem.

• 한국수산과학회지
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• 제19권2호
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• pp.147-154
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• 1986

양식방어(Seriola quinqueradiata)에 vibrio병이 발생하여 상당한 피해를 입고 있다. 본 연구는 양식방어에 발생한 vibrio병의 원인균에 대한 생화학적 성상, 생물학적 성상, 약제감수성및 병원성을 검토한 것으로 결과는 아래와 같다. 1. 병어의 신장에서 원인균을 분리하였으며 생화학적 성상, 생물학적 성상을 조사하여 Vibrio sp.로 동정하였다. 2. 분리균이 발육할 수 있는 NaCl농도는 $0.5{\sim}7\%$(최적농도; $3\%$), pH의 경우는 $6{\sim}10$(최적; 9)였다. 3. 분리균은 tetracycline, chloramphenicol, gentamycin에 감수성을 나타내었으나, sulfisomezole, sulfisoxazole이는 감수성이 없었다. 4. 수온에 따르는 병원성은 $25^{\circ}C$에서는 모든 접종어에 병원성을 나타냈으나, $15^{\circ}C$에서는 방어만 병원성을 나타냈다.

• Fisheries and Aquatic Sciences
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• 제7권3호
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• pp.141-147
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• 2004

Sixteen polycyclic aromatic hydrocarbons (PAHs) were analyzed in seventy marine organisms (40 species) from the Korean coast. PAHs were present in all the organisms. The level of total PAHs in the organisms varied from 0.45 to 224 ng/g dry weight and the carcinogenic PAHs varied from 0.05 to 49.8 ng/g dry weight. The PAHs residues according to the marine organisms showed a highest content in bivalve species, and followed by crustaceans, cephalopods, fish and gastropods. Human dietary intake of total PAHs through marine organism in Korea was estimated to be 4.12 ng/kg body weight/day and 0.67 ng/kg body weight/day for carcinogenic PAHs. The relative contributions of individual species to the total dietary intake of PAHs were in the order of bivalves $(53.4{\%})$, fish $(21.9{\%})$, crustaceans $(15.3{\%})$, cephalopods $(8.8{\%})$ and gastropods $(0.6{\%})$. Daily dietary intake of $PAH_{TEQ}$ expressed as a TEQ value was estimated to be 0.13 pg TEQ/kg body weight/day, which did not exceed a tolerable daily intake (TDI) proposed by the KFDA and the WHO as well as the UK toxicity committee. Lifetime cancer risk due to ingestion of marine species by the Korean adult was evaluated using the equation estimating exposure of food ingestion. Although approximately $23{\%}$ of cumulative frequency of the sampled marine species exceeded the cancer risk guideline, lifetime cancer risk associated with marine organism consumption was negligible. Results indicate that dietary intake of PAHs through the consumption of the Korean marine organisms seems to be safe for human ingestion with negligible cancer risk.

• 수산해양교육연구
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• 제28권1호
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• pp.211-220
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• 2016

Species composition of aquatic organism in the marine ranching area of Gunsan, Korea were investigated using shrimp beam trawl from May to December in 2010. A total of 91 species, $98,127ind./km^2$ and $877.6kg/km^2$ of aquatic organism were collected. Among them, species were included 60 species in Pisces, 21 in Crustacea and 10 in Mollusca. The individual dominant species, occupying over 10% of total individuals, were Latreutes anoplonyx($47,327ind/km^2$, 48.23%), Crangon hakodatei($11,578ind./km^2$, 11.80%) and Trachysalambria curvirostris($10,237ind./km^2$, 10.40%). And the biomass dominant species, occupying over 9% of total biomass, were Paralichthys olivaceus($135kg/km^2$, 15.4%), Okamejei kenojei($98.2kg/km^2$, 11.2%) and Portunus tribuberculatus($84.8kg/km^2$, 9.6%). From the cluster and MDS analysis based on Bray-Curtis similarity matrix of fourth root transformed data of number of species and individuals per unit area collected more than two times during this survey by each month and station was divided into three different groups. Group A showed seasonal similarity of characteristic of distribution in August and November, Group B in December and Group C in May.

• 한국수산과학회지
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• 제49권3호
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• pp.351-358
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• 2016

The aim of this study was to verify the performance of Teleaulax amphioxeia as prey for Brachionus plicatilis and for the larvae of the bivalves (Pacific oyster Crassostrea gigas and Manila clam Ruditapes philippinarum) to select the best of five T. amphioxeia strains. The five strains of T. amphioxeia were collected from the coasts of Korea, purecultured and then fed to the rotifer and bivalve larvae. The density and fecundity rate of the rotifer fed on strain 01 were the highest. The instantaneous growth rate of the rotifer fed strain 08-2 was the highest. The maximum density and fecundity rate of the rotifer fed strain 08-2 were significantly higher than those fed Chlorella ellipsoidea, while instantaneous growth rate showed the opposite pattern. Survival rates of the Manila clam larvae fed each strain of Teleaulax showed no significant difference between strains. Survival rates of the Pacific oyster fed Teleaulax were significantly higher than those fed Chaetoceros sp., but lower than those fed I. galbana. This study indicates that strain 08-2 showed better growth for B. plicatilis and better survival for the bivalve larvae than did the other strains, and that T. amphioxeia can also be used as a prey organism for rotifers or bivalve larvae.

• 한국수산과학회지
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• 제44권5호
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• pp.506-515
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• 2011

To elucidate demersal organism community structure, abundance, and biomass by period and region in the Uljin marine ranching area, otter trawl surveys were conducted at six stations from February 2008 to December 2009. During the survey period, a total of 118 species was collected in the Uljin marine ranching area, including 76 pisces, 27 mollusca and 15 crustaceans. The average abundance of individuals was 806 ind./$m^2$ and average biomass was 31,853 g/m2. The numbers of species, species richness, individuals per area and biomass per area were higher in 2009 than in 2008. Cluster and MDS analysis, based on a Bray-Curtis similarity matrix of fourth root transformed data of number of species and individuals per area, showed division into two different groups of demersal organisms in 2008 (Group 1) and one demersal organism community in 2009 (Group 2). From cluster and MDS analysis using the similarity of the demersal community among six stations, the Uljin marine ranching area was divided into Group A (St. 2, St. 4, St. 5 and St. 6) and Group B (St. 1 and St. 3).

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2005년도 추계학술대회 논문집
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• pp.181-185
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• 2005

선박에서 발생되는 밸러스트수를 전처리하기 위하여 수중에 포함되어 있는 입자상 오염물질과 수중생물체를 자동역세척 여과장치를 이용하여 처리한 결과를 나타내었다. 밸러스트수를 처리하기 위한 전처리의 장점은 입자상이 오염물질을 제거하여 후처리공정의 처리효과를 높이는데 있다. 여과필터를 회전하여 20RPM 에서는 40.5cmHg이고 40RPM 에서는 36.6cmHg이며 60RPM 이상에서는 35cmHg로 나타나 연속적으로 여과할 수 있었다. 필터의 막힘현상으로 역세 회복압력과 역세간격은 시간의 경과에 따라 조금씩 좁아지고 처리유량도 감소하는 것을 관찰할 수 있었다. 회분식 여과처리공정은 자동 역세척을 수행하는 공정에 비해서 처리효율의 저하가 일어났다. 여과처리를 통하여 $80{\mu}m$이상의 식물성 플랑크톤과 동물성 플랑크톤을 포함하는 수중생물체를 제거할 수 있었다. 연구결과를 통하여 밸러스트수 처리에 적용 가능한 기술임을 알 수 있었다.

• 한국수산과학회지
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• 제27권3호
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• pp.292-298
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• 1994

식중독 원인균으로 알려진 Vibrio mimicus(V. mimicus)의 분포 상태와 이의 생리적 특성을 조사하기 위하여 1993년 1월부터 9월까지 민락동과 광안리 해수욕장 부근의 해수를 채취하여 V. mimicus의 월별 분포 상태를 실험하였으며, 여기서 분리된 균주 중에서 항생제 내성이 강한 균으로 분리, 동정된 V. mimicus K-1의 생리적 성질과 배양 특성을 조사하였다. 수온이 $16.3^{\circ}C$인 4월부터 검출되기 시작하여 8월까지 검출되었으나, 수온이 $15^{\circ}C$ 이하인 겨울철(1, 2, 3월)에는 전혀 검출이 되지 않았다. 분포 정도는 최소 0.4(MPN/100ml)에서 최고 70((MPN/100ml)으로 지역에 따라 차이가 있었다. V. mimicus K-1은 $37^{\circ}C$, pH 7.5, 그리고 염도 $0.5\%$에서 각각 최대의 증식율을 보였다. 그리고 항생제 (colistin, erythromycin, bacitracin, tetracycline, penicillin)에 대해서 내성을 나타내었다. 자외선(30W, 50m)에 대해서는 상당히 내성이 약했으며, 70초간 조사 후에는 검출이 되지 않았다. 열에 의한 치사율은 $50^{\circ}C$에서 5.7분, $60^{\circ}C$에서 2.1분, 그리고 $70^{\circ}C$에서는 0.7분 만에 최초균수의 $90\%$이상이 사멸하였다.

• 한국어병학회지
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• 제28권2호
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• pp.71-78
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• 2015

In this study, we conducted to the development of a rapid antimicrobial susceptibility test method using WST-1 which is known to water-soluble tetrazolium salt, in order to rapidly response against bacterial diseases in fish. Eight of antibiotics which are permissioned for marine organism from government were used to rapid antimicrobial susceptibility testing using the WST-1. As a result, a similar tendency was verified compare to conventional antibiotic susceptibility test results. Generally, the antibiotic susceptibility test method required about 3 days (72 hours) for determine the effective antibiotics, however, we have confirmed that the our method using WST-1 was required at least 36 hours in this study. Consequentially, our method will contribute to development of rapid antimicrobial susceptibility testing for bacterial diseases in fish.

• 한국양식학회지
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• 제12권4호
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• pp.303-307
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• 1999

Copepod is useful live food organism in the larval rearing. However of fin fish, culcures of copepod species (Specially calanoida and cyclopoida) were very difficult under artificial management conditions. In this study, we report successful culture of a free swimming cyclopodia copepod species (Apocyclops sp.) by easily baker's yeast as food. The maximum culture density of Apocyclops sp. was observed 11,200±2,660 ind/ℓ during the 30 days culture periods in the 1.5ℓ culture tanks. The nauplii were growth up maximum density 6,400±432 ind/ℓ on day 16th. The number of egg carring female was observed maximum density (666.7 ind./ℓ) of two times after 6days and 28 days of initial culture.