• Proceedings of the Korean Society of Marine Engineers Conference
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• 2006.06a
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• pp.149-150
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• 2006

Displayed result that handle particle contaminant and hydrospace organism included in number of ballast that is happened in ship using automatic back washing filter. Reason that remove first contaminant that is included in number of ballast is that heighten processing effect of after processing process. of the filter. Another advantage is to drop oft the solids with controlling revolution of drum screen in pretreatment filtration process. The fact that it is easy to attach and detach a several type of screen for getting the expected water quality is another advantage. Filter rotation speed at 1.0rpm is filter resistance 3.0bar and 3.0rpm is filter resistance 2.8bar. Filter out impurities from ballast water over 6.0rpm is filter resistance 2.6bar and 10rpm is filter resistance 2.5bar. Filtration system removal aquatic organism over $80{\mu}m$ in ballast water. This study shows that the filtration treatment system has a potential for the treatment of ballast water.

• Fisheries and Aquatic Sciences
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• v.12 no.2
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• pp.138-151
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• 2009

We quantitatively investigated compositions of fish and invertebrate bycatch in Korean shrimp fisheries. We sampled shrimp trawl bycatch from 48 commercial trawls over 1 year. These samples contained 108 taxa from 50 families, with 60 fish taxa contributing 51.2% of the total biomass and 48 invertebrate taxa 48.8%. However, 86.32% of the total individual organism density comprised invertebrates, while individual fishes made up the remainder. Within the bycatch, two fish species varied in population size structure by season, suggesting recruitment is seasonal in these taxa. Overall general catch characteristics (total bycatch biomass and organism density) varied significantly by season and tide, and a significant interaction effect was observed (season$\times$tide) on total density, but not on total biomass. The data collected will be used in designing a program of long-term bycatch monitoring.

• Journal of Aquaculture
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• v.16 no.3
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• pp.196-201
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• 2003

Sessile organisms such as the oyster Crassostrea gigas have been given much attention as a potential biomonitoring indicator to assess the impact of toxicants on aquatic organism. In this study, we exposed cells isolated from gill of oyster (Crassostrea gigas) to hydrogen peroxide in vitro. In addition oysters were in vivo exposed to pyrene and benzo(a)pyrene at various concentrations for 2 weeks. Comet assay was used to detect DNA single strand breaks and to investigate the application of this technique as a tool for aquatic biomonitoring. Hydrogen peroxide increased DNA single strand break with increasing concentration after 30 minutes exposure in vitro. Pyrene and benzo(a)pyrene caused DNA damage only at very high concentration (100 $\mu\textrm{g}$/L or 1000 $\mu\textrm{g}$/L) at two week exposure in vivo. DNA damage was relatively higher at hemocyte than at gill. It suggested that metabolized PAHs are transferred to hemolymph from digestive gland which have a relatively high enzyme activity, and attacked the DNA of hemocyte, while gill accumulated PAHs without degrading them to their metabolites due to low enzyme activity at gill. Both in vitro and in vivo exposure experiments showed that the comet assay is an effective tool on screening whether the organism are exposed to genotoxic contaminants.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.54 no.4
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• pp.306-314
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• 2018

Species composition and seasonal variation of aquatic organism at Baekryeong-do, Korea were investigated using pots in 2016. A total of 42 species, 90,050 individuals and 2,351,666 g of aquatic organism were collected. Among them, there were 12 species and 3,826 individuals of fish, 15 species and 51,237 individuals of crustaceans, 2 species and 54 individuals of cephalopods, 7 species and 33,981 individuals of gastropods, 2 species and 757 individuals of echinodea, 2 species and 187 individuals of shellfish, and 1 species and 8 individuals of holothuroidea. The dominant species in number of individuals were Pagurus ochotensis, Neptunea cuming, Cancer gibbosulus, and Buccinum yokomaruae, which accounted for 70.0% of the total number individuals collected. The dominant species in biomass were Neptunea cuming, Portunus trituberculatus, Cancer gibbosulus, and Pagurus ochotensis, which accounted for 65.3% of the total biomass collected.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.4
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• pp.387-400
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• 2012

Species composition and seasonal variation of aquatic organism in the coastal waters off Taean, Korea were investigated using catches by commercial fishing of the pot and gill net from April 2010 to January 2011. A total of 58 species, 10,602 individuals and 547,569g of aquatic organism were collected during the study. Aquatic organism were composed of 22 species of Pisces, 20 species of Crustacea, 8 species of Gastropoda, 3 species of Echinodea, 2 species of Cephalopoda and Bivalvia, and 1 species of Holothuroidea. 41 species in 9 orders and 22 families were collected by the pot, and 28 species in 15 orders and 22 families were collected by the gill net. Volutharpa ampullacea was the most dominant in the pot, while Okamejei kenojei was the most dominant in the gill net. These two species accounted for 73.9% in the number of individuals, and 64.4% in biomass. The number of species was high in spring in the pot, and autumn in the gill net. The number of individuals and biomass were highest in winter and summer respectively in the pot, and summer in the gill net. The number of species of the consignment quantity caught by commercial fishery of the pot and gill net in Mohang was 12 and 14 respectively. Cluster analysis based on species composition of the 18 most common species showed that the species were separated into 3 different group. Group A was composed of species which were abundant in spring, group B was composed of species which were abundant in summer and autumn, and group C was composed of year-round residents.

• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.535-539
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• 2016

The prevalence of pathogenic bacteria such as Streptococcus parauberis (Sp), Streptococcus iniae (Si), and Edwardsiella tarda (Et) in flounder fish farms in Jeju Island and their management by gallium treatment was studied. Sp, Si, and Et were found to exhibit a low rate of cell growth and high biofilm formation. Hence, in the present study, cell growth and biofilm formation were measured spectrophotometrically 72 h after the addition of different concentrations of gallium (2, 4, or 8 mg/ml). In addition, cell death was measured by resazurin and propidium iodide staining assays. The results showed that bacterial cell death increased and biofilm formation decreased with an increasing concentration of gallium. Hence, the present study signifies that the use of gallium against bacterial pathogens could be useful for disease management in flounder farms.

• Journal of Life Science
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• v.28 no.10
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• pp.1179-1185
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• 2018

Recently, Sargassum horneri, the marine weed inhabiting the shoreline, beach, and littoral sea area, has caused serious damage to intensive aquaculture farms particularly those around Jeju Island, South Korea. The purpose of this study was to investigate the diversity of microorganisms in Sargassum horneri and to provide basic data on ecological problems by identifying microbial functions. A total of 88 isolates were identified by 16S rRNA sequencing. Proteobacteria was the dominant phylum accounting for 88%, including class ${\alpha}-proteobacteria$, six genera, and ten species. The dominating genus, Pseudobacter, accounted for 40% in Pseudorhodobacter, 20% in Paracoccus, and the remaining at 10% each were Rhizobium, Albirhodobacter, Skermanella, and Novosphingobium. Class ${\beta}-proteobactera$ included five genera and ten species. Genus Hydrogenophaga accounted for 50%, while genus Azoarcus accounted for 20%, and the remaining Oxalicibacterium, Duganella, and Xenophilus were 10% each. Class ${\gamma}-proteobacteria$ with 13 genera and 57 species, accounted for 74% in phylum Proteobacteria, 23% in Shewanella, 19% in Cobetia, 12% in Pseudomonas, 4% each in Vibrio and Serratia, and 2% each in Rheinheimera, Raoultella, Pantoea, Acinetobacter, Moraxella, and Psychrobacter genera. In addition, Actinobacteria with two species of Nocardioides genera accounted for 50%, and Bacteroidetes accounted for 33%, with three genera and five species that included Lacihabitans and Mariniflexile. The remaining Dyadobacter, Cellulophaga, and Ferruginibacter genera each accounted for 11%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.3
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• pp.283-289
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• 2003

The effect on kidney, gill, and hepatopancreas of juvenile olive flounder, Paralichthys olivaceus were investigated by histological method under limit concentration of effulent of PCBs for 60 days. The free surface of epithelial cell layer in the renal tubules of kidney showed a strong positive reaction in alcian blue during all exposure period. There were also observed swelling of hemocyte in glomerulus and macrophage. At 60 days after exposure, epithelium of the renal tubules was fused and some of organism $(20\%)$ showed damage of glomerulus and eosinophlic cell in epithelium of the renal tubules. The phenomena such as the activation and increase of the chloride cell, and swelling of hemocyte in the capillary of gill were observed during all exposure period. At 60 days after exposure, swelling of the gill filament and mucous cell appeared in gill and some of organism $(30\%)$ showed swelling of the gill lamellar. Swelling of hemocyte in the capillary of hepatopancreas was observed and the free surface of epithelial layer of the bile duct showed positive reaction in alcian blue during all exposure period. Degenerated zymogen in the pancreas and swelling of the hepatocyte were occurred at 60 days after exposure.

• Journal of fish pathology
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• v.32 no.1
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• pp.29-35
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• 2019

A monitoring was performed to survey the mortalities that had occurred in the aquaculture farms of olive flounder (Paralichthys olivaceus) in South Korea from 2015 to 2017. The indirect inquiry for entire farms and the sample survey for selected farms were carried out. The aquatic organism disease inspectors, who have a national license for the diagnosis and prevention of aquatic organism diseases and a have close relationship with the farms, investigated the rates and causes of mortalities according to the standard manual. The mortality rate by sample survey of farms in 2015, 2016, and 2017 were 24.78% (Chunnam: 17.86%, Jeju: 28.69), 30.19% (Chunnam: 24.45%, Jeju: 32.65), and 21.59% (Chunnam: 10.57%, Jeju: 26.00%), respectively. The major cause of mortality was scuticociliatosis, and the mortality caused by viral hemorrhagic septicemia and emaciation disease (Jeju) were also high. Our results can contribute to effective establishment prevention of epidemics system and acquired status as a disease-cleansing country.

• Journal of fish pathology
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• v.18 no.2
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• pp.125-131
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• 2005

Amoebic gill disease of flounder, Paralichthys olivaceus was diagnosed at commerical culture facility in South Korea. The amoeba was identified as a species of the genus Neoparamoeba based on the morpholgical characteristics of trophozoites. Transmisson electron microscopy revealed the presence of a symbiotic organism, parasome in the cytoplasm and dense glycocalyx on the surface of the trophozoites. They lacked the boat-shaped microscales on the surface and contained numerous vacuoles and channels, mitochondria in the cytoplasm. Colonization of amoebae on gill tissue elicited extensive fusion and hyperplasia of gill lamella.