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Histological Response of Kidney, Gill and Hepatopancreas of the Juvenile Olive Flounder, Paralichthys olivaceus after PCBs Exposure

PCBs 노출에 따른 넙치(Paralichthys olivaceus) 치어의 신장, 아가미 및 간췌장의 조직학적 반응

  • KIM Jae-Won (Department of Aquatic Life Medicine, Pukyong National University) ;
  • JEE Jung-Hoon (Department of Aquatic Life Medicine, Pukyong National University) ;
  • KANG Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University) ;
  • LEE Jung-Sik (Department of Fish Aqualife Medicine, Yosu National University) ;
  • CHIN Pyung (Marine Biology, Pukyong National University)
  • 김재원 (부경대학교 수산생명의학과) ;
  • 지정훈 (부경대학교 수산생명의학과) ;
  • 강주찬 (부경대학교 수산생명의학과) ;
  • 이정식 (여수대학교 어병학과) ;
  • 진평 (부경대학교 자원생물학과)
  • Published : 2003.06.01

Abstract

The effect on kidney, gill, and hepatopancreas of juvenile olive flounder, Paralichthys olivaceus were investigated by histological method under limit concentration of effulent of PCBs for 60 days. The free surface of epithelial cell layer in the renal tubules of kidney showed a strong positive reaction in alcian blue during all exposure period. There were also observed swelling of hemocyte in glomerulus and macrophage. At 60 days after exposure, epithelium of the renal tubules was fused and some of organism $(20\%)$ showed damage of glomerulus and eosinophlic cell in epithelium of the renal tubules. The phenomena such as the activation and increase of the chloride cell, and swelling of hemocyte in the capillary of gill were observed during all exposure period. At 60 days after exposure, swelling of the gill filament and mucous cell appeared in gill and some of organism $(30\%)$ showed swelling of the gill lamellar. Swelling of hemocyte in the capillary of hepatopancreas was observed and the free surface of epithelial layer of the bile duct showed positive reaction in alcian blue during all exposure period. Degenerated zymogen in the pancreas and swelling of the hepatocyte were occurred at 60 days after exposure.

Keywords

References

  1. Braunbeck, T. and A. Volkl. 1991. Induction of biotransformation in the liver of eel, Anguilla anguilla L. by sublethal exposure to dinitro-o-cresol: An ultrastructural and biochemical study. Ecotoxicol. Environ. Saf., 21, 109-127 https://doi.org/10.1016/0147-6513(91)90014-G
  2. Braunbeck, T., T. Gorge, V. Stoach and R. Nagel. 1990. Hepatic steatosis in zebra fish, Brschydsnio rerio induced by long-term exposure to $\gamma$ hexachlorocyclohexane. Ecotoxicol. Environ. Saf., 19, 355-374 https://doi.org/10.1016/0147-6513(90)90036-5
  3. Eurell, J.A. and W.E. Haensly. 1982. The histology and ultrastructure of the liver of atlantic croaker, Micropogon undulatus. j. Fish Biol., 21, 113-125 https://doi.org/10.1111/j.1095-8649.1982.tb02829.x
  4. Giesy J.P. and K. Kannan. 1998. Dioxin-like and nondioxinlike to effects of polychlorinated biphenyls (PCBs): Implications for reassessment. Crit. Rev. Toxicol., 28, 511-569 https://doi.org/10.1080/10408449891344263
  5. Goksoyr, A and L. F$\ddot{o}$rlin. 1992. The cytochrome P-450 system in fish, aquatic toxicology and environmental monitoring. Aquat. Toxicol., 22, 287-312 https://doi.org/10.1016/0166-445X(92)90046-P
  6. Grinwis, G.C.M., A. Boonstra, E.J. Brandhof, J.A.M.A. Dormans, M. Engelsma, R.V. Kuiper, H. Loveren, P.W. Wester, M.A. Vaal, A.D. vethaak and J.G. Vos. 1998. Short-term toxicity of bis (tri-n-butyltin) oxide in flounder, Platichthys flesus: Pathology and immune function. Aquat. Toxicol., 42, 15-36 https://doi.org/10.1016/S0166-445X(97)00100-8
  7. Harvey, G.R., H.P. Miclas, V.T. Bowen and W.G. Strinhauer. 1974. Observations on the distribution of chlorinated hydrocarbons in Atlantic ocean organisms. J. Mar. Res., 32, 103-118
  8. Hinton, D.E., J.E. Klaunig and M.M. Lipsky. 1978. PCB-induced alterations in teleost liver. A model for enviromental disease in fish. Mar. Fish Rev., 40, 47-50
  9. Jensen, S. 1966. Report of a new chemical hazard. New Scientist, 32, 612
  10. Johansson, N., A. Larsson and K. Lewander. 1972. Metabolic effects of PCB (polychlorinated biphenyls) on the brown trout, Salmo trutts. Compo Gen. Pharmacol., 3, 310-314 https://doi.org/10.1016/0010-4035(72)90009-2
  11. Joo Y.J., M.O. Gu, J.H. Jeong and G.H. Jeong. 1998. Distribution status of PCBs in fishes of hoedong reservoir and in the sediments of upper stream of the sooyoung river. Environ. Anal., 1, 75-82. (in Korean)
  12. Kalmaz, E.V. and G.D. Kalmaz, 1979. Transport distribution and toxic effects of polychlorinated biphenyl in ecosystems. Review. Ecol. Model, 6, 223-251 https://doi.org/10.1016/0304-3800(79)90014-0
  13. Kang J.C. and K.S. Cho. 2001. Changes of hepatic microsomal cytochrome P450 monooxygenase system in nile tilapia, Oreocbromis niloticus exposed to PCBs. J. Kor. Fish Soc., 34, 194-198. (in Korean)
  14. Kang, J.C., J.S. Lee and J.H. Jee. 1999. Ecophysiological responses and subsequent recovery of the olive flounder, Paralichthys olivaceus exposed to hypoxia and iron. J. Kor. Fish. Soc. 32, 699-705. (in Korean)
  15. Lee, J.C., J.C. Kang, Y.K. Shin, K.H. Ma and P. Chin. 2001. Histological responses of the flounder, Paralichthys olivaceus exposed to copper. J. Fish Pathol., 14, 81-90. (in Korean)
  16. Mallatt, J. 1985. Fish gill structural changes induced by toxicants and other irritants: a statixtical review. Can. J. Fish. Aquat. Sci. 42, 630-648 https://doi.org/10.1139/f85-083
  17. Miranda, C.L., M.C. Henderson, J.L. Wang, H.S. Chang, J.D. Hendricks and D.R. Buhler. 1992. Differential effects of 3, 4, 5, 3', 4', 5'-hexachlorobiphenyl (HCB) on interrenal steroidogenesis in male and female rainbow trout, Oncorhynchus mykiss. Comp. Biochem. Physiol., 103C, 153-157
  18. Morrison, C.M. and P.H. Odense. 1978. Distribution and morphology of the rodlet cell in fish. J. Fish. Res. Bd Can. 35, 101-116 https://doi.org/10.1139/f78-014
  19. Muir, D.C.G., R.J. Norstrom and M. Simon. 1988. Organochlorine Contaminants in arctic food chains: Accumulation of specific polychlorinated biphenyls and chlordanerelated compounds. Environ. Sci. Technol., 22, 1071-1079 https://doi.org/10.1021/es00174a012
  20. Park C. K. and E. C. Hwang. 1982. Evaluation of polychlorinated biphenyls and organochlorine insecticide residues in waters, sediments and crucian carps in soho lake. Korean J. Environ. Agric. 1, 105-115. (in Korean)
  21. Reimschuessel, R., O.B. Richard, B.M. Eric and M.L. Michael. 1989. Renal histopathological changes in the goldfish, Carassius auratus after sublethal exposure to hexachlorobutadiene. Aquat. Toxicol., 15, 169-180 https://doi.org/10.1016/0166-445X(89)90014-3
  22. Sivarajah, K., C.S. Franklin and W.P. Williams. 1978. Some histopathological effects of Aroclor 1254 on the liver and gonads of rainbow trout, Salmo gslrdncri and carp, Cyprinus carpio. J. Fish. Biol., 13, 411-414 https://doi.org/10.1111/j.1095-8649.1978.tb03448.x
  23. Teh, S.J., S.M. Adams and D.E. Hinton, 1997. Histopa- thologic biomarkers in feral freshwater fish populations exosed to different types of contaminant stress. Aquat. Toxicol., 37, 51-70 https://doi.org/10.1016/S0166-445X(96)00808-9
  24. Temmink, J., P. Bouwmeister, P. de Jong and J. van den Berg. 1983. An ultrastructural study of chromate- induced hyperplasia in the gill of rainbow trout, Salmo gakdneri. Aquat, Toxicol., 4, 165-179 https://doi.org/10.1016/0166-445X(83)90053-X
  25. Wester, P.W. and J.H. Canton. 1987. Histopathological study of Poecilis reticulate, guppy after long-term exposure to bis (tri-n-butyltin) oxide (TBTO) and di-n-butyltin-dichloride (DBTC). Aquat, Toxicol., 10, 143-165 https://doi.org/10.1016/0166-445X(87)90020-8
  26. Yasutake W.T. and J.H. Wales. 1983. Microscopic anatomy of salmonids: An atals. Department of the interior fish and wildlife service washington D.C. U.S.A., pp. 97
  27. You, K.H., C.H. Choi, R.S. Choe and Y.K. Deung. 1978. Ultrastructural studies on cadmium poisoning in the liver, kidney and gills of Csrsssius csrsssius. Korean J. Electron Microscopy, 8, 33-48. (in Korean)
  28. Yun Y.P., S.K. Lee, Y.H. Kim, W.S. Kang, Y.T. Chi and S.C. Lee. 1994. Adverse effects of PCB (Aroclor 1254) on domestic freshwater fish. Kor. J. Limnol., 27, 291-296. (in Korean)