• The Journal of Korean Physical Therapy
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• v.31 no.4
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• pp.236-241
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• 2019

Purpose: This study examined the effects of aquatic proprioceptive neuromuscular facilitation pattern exercise on the balance, gait ability, and depression in patients with chronic stroke. Methods: Thirty patients with chronic stroke were assigned randomly to an experimental (n=15) or control (n=15) group. The experimental group performed aquatic proprioceptive neuromuscular facilitation pattern exercise. The control group performed ground proprioceptive neuromuscular facilitation pattern exercise. Training was conducted once a day for 30 minutes, five days per week for six weeks. The balance ability was measured using the Berg balance scale. The gait ability was measured using the 10 Meter walk test. Depression was measured using the Beck depression inventory. Results: As a result of a comparison within groups, the experimental and control group showed a significant difference for balance, gait ability, and depression after the experiment (p<0.05). In a comparison between the two groups, the experimental group, in which aquatic proprioceptive neuromuscular facilitation pattern exercise was applied, showed more significant changes in balance, gait ability, and depression than the control group (p<0.05). Conclusion: Based on these results, aquatic proprioceptive neuromuscular facilitation pattern exercise effectively improved the balance, gait ability and depression in patients with chronic stroke.

• Journal of Veterinary Science
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• v.21 no.2
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• pp.31.1-31.5
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• 2020

In this study, whiteleg shrimp (Penaeus vannamei) imported from Vietnam were collected from South Korean markets, and examined for 2 viruses: infectious hypodermal and hematopoietic necrosis virus (IHHNV, recently classified as decapod penstyldensovirus-1), and white spot syndrome virus (WSSV). Among 58 samples, we detected IHHNV in 23 samples and WSSV in 2 samples, using polymerase chain reaction and sequencing analyses. This is the first report of IHHNV and WSSV detection in imported shrimp, suggesting that greater awareness and stricter quarantine policies regarding viruses infecting shrimp imported to South Korea are required.

• PNF and Movement
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• v.20 no.2
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• pp.243-251
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• 2022

Purpose: This study aimed to compare the effects of aquatic and land dual-task training on balance, gait, and depression in chronic stroke patients. Methods: A total of 24 patients diagnosed with chronic stroke were the subjects. They were assigned to either the experimental group (n = 12) or the control group (n = 12). The experimental group performed aquatic dual-task training, while the control group performed land dual-task training. The aquatic and land dual-task training sessions were conducted once a day for 30 min, 5 days per week, for 6 weeks. Balance was measured using the Berg balance scale. Gait was measured using the Timed Up and Go Test. The Beck's Depression Inventory was used to measure depression. Results: Both the experimental and control groups showed significant differences in balance, gait, and depression after the intervention (p < 0.05) in the within-group comparisons. It was found that the experimental group showed more significant differences in balance, gait, and depression than the control group (p < 0.05) when the two groups were compared. Conclusion: It can be concluded that aquatic dual-task training effectively improved the balance ability, gait ability, and chronic stroke patients' depression based on these results.

• Korean Journal of Microbiology
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• v.51 no.4
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• pp.382-388
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• 2015

Eleven protease-producing bacteria were isolated from the organisms' external shells and the inorganic materials collected from intertidal zone of Jeju Island, Republic of Korea. The samples were diluted serially, inoculated on Zobell agar plates with 1% skim milk and incubated at $20^{\circ}C$. Clear zone forming colonies were selected as protease-producing bacteria and each strain was identified based on the phylogenetic analysis with their 16S rDNA sequences. Strains JJM125, JJM129, YG47 and YG49 belong to the marine bacterial genus Pseudoalteromonas; strain JJM122 belong to the genus Microbulbifer; strains YG51, YG52, YG62 and YG63 belong to the genus Vibrio; and strain YG65 belong to genus Bacillus. Hence, the present study suggests that these protease producing bacteria could be further used to develop new varieties of protease with various biotechnological applications.

• Fisheries and Aquatic Sciences
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• v.23 no.8
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• pp.20.1-20.18
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• 2020

Background: Olive flounder, Paralichthys olivaceus, is an economically important aquaculture species in Korea. Olive flounders have been heavily damaged by streptococcal infections every year and are treated with antibiotics. However, antibiotic abuse is causing the emergence of resistant strains, and to overcome this, research has shown that new antibiotics must be applied. Tylosin is a relatively safe antibiotic and has good activity against Gram-positive bacteria and mycoplasma. We studied the therapeutic effects and side effects of tylosin on Streptococcus parauberis-infected olive flounder. Methods: After artificial infection of olive flounder with S. parauberis SPOF18J3, an appropriate dose of tylosin was confirmed by intramuscular injection (I.M.) at 2.5, 5, 10, and 15 mg/kg, and oral administration at 10 and 20 mg/kg. After I.M. and oral administration dosing of tylosin, side effects were confirmed by serological analysis, histopathological analysis, and median lethal dose (LD₅₀) analysis at both an appropriate concentration and a high concentration. Statistical analysis was performed using one-way analysis of variance (ANOVA) and Tukey's test (p < 0.05). Results: The appropriate I.M. and oral administration concentration of tylosin administered to olive flounder infected with S. parauberis SPOF18J3 was found to be 10 mg/kg. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were showed not significantly different between the control group and the experimental groups. The histopathologic results showed mild inflammatory responses in muscle and tubular vacuolization and tubular atrophy appeared, but there were no significant differences between the groups. The LD₅₀ was confirmed to be 461 mg/kg. Conclusion: In this study, an effective treatment method was provided by verifying the treatment effects and side effects of tylosin in olive flounder infected with S. parauberis, which can be applied directly to aquaculture sites. In addition, these results may be used as a reference for evaluation required upon request to obtain approval for tylosin antibiotics as fishery antibiotics in Korea. After approval, it is possible that a fishery disease manager will be able to prescribe and sell the antibiotic tylosin.

• Korean Journal of Ecology and Environment
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• v.46 no.3
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• pp.395-408
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• 2013

Environmental condition can induce changes in early life-history traits in order to maximise the ecological fitness. Here I investigated how temperature change and variation in human aquatic activity/behaviour affect early life-history consequences in fish using a dynamic-state-dependent model. In this study, I developed a general fish's life-history model including three life-history states depend-ing on foraging activity, such as body mass, mass of reproductive tissue (i.e., gonadal development) and accumulated stress (i.e., cellular or physiological damage). I assumed the level of foraging activity maximises reproductive success-ultimately, fitness. The model predicts that growth rate, development of reproductive tissues and damage accumulation are greater in higher temperature whereas higher human aquatic activity rapidly reduced the growth rate and development of reproductive tissue and increased damage accumulation. While higher foraging activity in higher temperature is less affected by human aquatic activity, the foraging activity in lower temperature rapidly declined with human aquatic activity. Moreover, lower survival rate in higher temperature or human aquatic activity was independent on mortality rate due to human aquatic activity or mortality rate when foraging activity, respectively. However, the survival rate in lower temperature or human aquatic activity was dependent on these mortality rates. My findings suggest that including of early life-history traits in relation to climate-change and human aquatic activity on the analysis may improve conservation plan and health assessment in aquatic ecosystem.

• Journal of fish pathology
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• v.22 no.3
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• pp.317-326
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• 2009

This study has shown the screening of anti-bacterial activity of three Indian medicinal plant choloroform : methanol (50:50) solvent leaf extracts (i.e. Azadirachta indica, Ocimum sanctum, and Curcuma longa) with different concentrations (10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 mg/ml) under in vitro conditions against fish pathogenic bacteria, Aeromonas hydrophila, Streptococcus iniae, Vibrio harveyi, V. anguillarum, and Edwardsiella tarda isolated from olive flounder farms, Jeju Island, South Korea. The anti-microbial activity of the A. indica and O. sanctum extracts yielded the zones of growth inhibition (ZI) was 3 and 1mm against A. hydrophila at concentration of 0.156 mg/ml when compared to that of tetracycline standard (3 mm). At highest concentration (10 mg/ml) of A. indica, O. sanctum, and C. longa, high inhibition was 9, 7, and 6 mm when compared to that of tetracycline (11 mm) against A. hydrophila. The minimum inhibitory concentration (MIC) of A. indica, O. sanctum, and C. longa at 0.156 mg/ml that yield 9, 10, and 13 CFU/ml for A. hydrophila, 16, 22, and 25 CFU/ml for S. iniae and 18, 22, and 23 CFU/ml for E. tarda compared to the tetracycline. At highest concentration (10 mg/ml) of the three extracts was better inhibiting the growth of A. hydrophila, S. iniae and E. tarda. A. indica, O. sanctum, and C. longa were determined to the potential antioxidant activityon the basis of their scavenging activity of the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. A. indica extract was 0.625 mg/ml which indicated that the strong anti-oxidant activity. However, O. sanctum and C. longa extracts showed weak anti-oxidant activity at this concentration. Hence, in vitro assay among the pathogens, A. hydropila is better inhibitory activity of the extracts. It is evident that the Indian medicinal plants extracts were subjected to its effectiveness against A. hydrophila, S. iniae, and E.tarda at low concentrations. The obtained results in the present study suggested that the Indian plant extracts is a prevention tools for Korean olive flounder aquaculture pathogens and its need further advance investigation.

• PNF and Movement
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• v.6 no.2
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• pp.1-10
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• 2008

Purpose : The purpose of this study was to investigate the effect of aquatic exercise applied PNF patterns on body composition and balance performance in people who have had a stroke. Methods : Forteen candidates who have all experienced a stroke were participating in a community based rehabilitation program, have been included in this study. The program was conducted three times weekly, 1 hour per session, for 10 consecutive weeks. Subjects were tested with body composition and 5 items of Berg's balance test at pre-training and post-training. Total balance indexes in 3 conditions were measured by K.A.T. 3000. The aquatic exercise applied PNF patterns was consisted of PNF patterns and various aquatic activities. Results : After ten weekends of aquatic exercise training, there were not significant difference in body composition(p>.05) except of muscular weight of affected lower extremity(p<.05). But edema index increased more than pre-training (p<.05). Subjects showed significant difference in Berg's balance test results except of 2 items of Berg's balance test (p<.05). Total balance index score when subjects opened their eyes and didn't hold the handle was decreased less than pre-training(p<.05). Conclusion : The results of this study showed that intervention of this aquatic exercise program applied PNF patterns could increase edema index and muscular weight of affected lower extremity and improve the balance performance in people who have had a stroke.

• The Journal of the Korea Contents Association
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• v.9 no.12
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• pp.674-682
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• 2009

The purpose of this study was to investigate the effect of aquatic gait training on plantar foot pressure, foot kinesiology and gait speed in right hemiplegic patients. The subject were 20 stroke patients who elapsed from 12 month to 24 month after stroke(aquatic gait training group(n=10), land gait training group(n=10)). This study measured plantar foot pressure, toe out angle, subtalar joint angle, gait speed from data of gate on 2m long measuring apparatus for RS-scan system(RS scan Ltd. German). This experiment performed in twice, before and after the aquatic gait training and land gait training. Collected data were statistically analyzed by SPSS Ver. 12.0 using descriptive statistics, paired t-test. Aquatic gait training group had more variety pressure area on their foot such as T1(Toe 1), HM(Heel medial), and HL(Heel lateral). But motion of subtalar joint flexibility and toe out angle decreased considerably and gate speed also increased. According to the result, aquatic gait training is considered as more effective way in foot stability and normal gait pattern than land gait training.

• The Journal of the Korea Contents Association
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• v.9 no.12
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• pp.656-664
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• 2009

The purposes of this study were to examine whether aquatic exercise has influence on the neuroplasticity and vestibulo-motor function in diffuse brain injury rats. 80 Sprague-Dawley rats were assigned to four groups; Group I: control group (n=20), Group II: aquatic exercise (n=20), Group III: treadmill exercise with change of velocity and inclination (n=20), Group IV: simple treadmill exercise (n=20). And we applied exercise each groups for 3 weeks except Group I. Before the rats were sacrificed to identify immunohistochemistry study at each time of measurement day, Rota-Rod test was given to assess changes in vestibulomotor function. then, the immunohistochemistry study of GAP-43 in discrete regions of the rat brain was performed to measure changes in neuroplasticity. The results demonstrate that aquatic exercise group is more effective than other groups. expression of GAP-43 and vestibulo-motor function were increased most in aquatic exercise group. Therefore, this study suggest that aquatic exercise may effective therapeutic approach to increase neuroplasticity and vestibulo-motor function in traumatic brain injury.