• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.596-606
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• 2019

N-acyl-homoserine lactone quorum sensing (AHL-QS) has been shown to regulate many physiological behaviors in Serratia marcescens MG1. In the current study, the effects of AHL-QS on the biosynthesis of acid and neutral products by S. marcescens MG1 and its isogenic ${\Delta}swrI$ with or without supplementing exogenous N-hexanoyl-L-homoserine lactone ($C_6-HSL$) were systematically investigated. The results showed that swrI disruption resulted in rapid pH drops from 7.0 to 4.8, which could be restored to wild type by supplementing $C_6-HSL$. Furthermore, fermentation product analysis indicated that ${\Delta}swrI$ could lead to obvious accumulation for acidogenesis products such as lactic acid and succinic acid, especially excess acetic acid (2.27 g/l) produced at the early stage of fermentation, whereas solventogenesis products by ${\Delta}swrI$ appeared to noticeably decrease by an approximate 30% for acetoin during 32-48 h and by an approximate 20% for 2,3-butanediol during 24-40 h, when compared to those by wild type. Interestingly, the excess acetic acid produced could be removed in an AHL-QS-independent manner. Subsequently, quantitative real-time PCR was used to determine the mRNA expression levels of genes responsible for acidogenesis and solventogenesis and showed consistent results with those of product synthesis. Finally, by close examination of promoter regions of the analyzed genes, four putative luxI box-like motifs were found upstream of genes encoding acetyl-CoA synthase, lactate dehydrogenase, ${\alpha}$-acetolactate decarboxylase, and Lys-like regulator. The information from this study provides a novel insight into the roles played by AHL-QS in switching from acidogenesis to solventogenesis in S. marcescens MG1.

• Journal of Internet Computing and Services
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• v.19 no.6
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• pp.1-7
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• 2018

This paper present an 8kbps ACLMS-MPC(Amplitude Compensation and Least Mean Square - Multi Pulse Coding) coding method integrated with ACFBD-MPC(Amplitude Compensation Frequency Band Division - Multi Pulse Coding) and LMS-MPC(Least Mean Square - Multi Pulse Coding) used V/UV/S(Voiced / Unvoiced / Silence) switching, compensation in a multi-pulses each pitch interval and Unvoiced approximate-synthesis by using specific frequency in order to reduce distortion of synthesis waveform. In integrating several methods, it is important to adjust the bit rate of voiced and unvoiced sound source to 8kbps while reducing the distortion of the speech waveform. In adjusting the bit rate of voiced and unvoiced sound source to 8 kbps, the speech waveform can be synthesized efficiently by restoring the individual pitch intervals using multi pulse in the representative interval. I was implemented that the ACLMS-MPC method and evaluate the SNR of APC-LMS in coding condition in 8kbps. As a result, SNR of ACLMS-MPC was 15.0dB for female voice and 14.3dB for male voice respectively. Therefore, I found that ACLMS-MPC was improved by 0.3dB~1.8dB for male voice and 0.3dB~1.6dB for female voice compared to existing MPC, ACFBD-MPC and LMS-MPC. These methods are expected to be applied to a method of speech coding using sound source in a low bit rate such as a cellular phone or internet phone. In the future, I will study the evaluation of the sound quality of 6.9kbps speech coding method that simultaneously compensation the amplitude and position of multi-pulse source.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.2
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• pp.284-292
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• 2003

In this paper, we carried out the numerical examination of the initial value setting-up method to extend the range of optimal step parameter in a adaptive system which is controlled by LMS algorithm. For initial value setting-up methods, the general method which select the initial value randomly and the other method which applies the approximate value obtained from the direct method to initial value, were used. And then, we compared to the ranges of step parameter setting, the convergence speeds of mean-square-error, and the stabilities during the convergence process when the initial values were applied to the optimal directivity synthesis problem. According to the numerical simulation results, the initial value setting-up method by means of the direct method provides wider range for the step parameter, more efficient capability for convergence and stability, and more error correction ability than the general method.

• Journal of Microbiology and Biotechnology
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• v.7 no.4
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• pp.229-236
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• 1997

A gene, $phbC_{2.4.1}$ encoding poly-3-hydroxybutyric acid (PHB) synthase of Rhodobacter sphaeroides 2.4.1 was cloned by employing heterologous expression in Escherichia coli. R. sphaeroides chromosomal DNA partially digested with MboI was cloned in pUC19 followed by mobilization into E. coli harbouring $phbA,B_{AC}$ in pRK415, which code for ${\beta}$-ketothiolase and acetoacetyl CoA reductase of Alcaligenes eutrophus, respectively. Two E. coli clones carrying R. sphaeroides chromosomal fragment of $phbC_{2.4.1}$ in pUC19 were selected from ca. 10,000 colonies. The PHB-producing colonies had an opaque white appearance due to the intracellular accumulation of PHB. The structure of PHB produced by the recombinant E. coli as well as from R. sphaeroides 2.4.1 was confirmed by [$H^{+}$]-nuclear magnetic resonance (NMR) spectroscopy. Restriction analysis of the two pUC19 clones revealed that one insert DNA fragment is contained as a part of the other cloned fragment. An open reading frame of 601 amino acids of $phbC_{2.4.1}$ with approximate M.W. of 66 kDa was found from nucleotide sequence determination of the 2.8-kb SaiI-PstI restriction endonuclease fragment which had been narrowed down to support PHB synthesis through heterologous expression in the E. coli harbouring $phbA,B_{AC}$. The promoter (s) of the $phbC_{2.4.1}$ were localized within a 340-bp DNA region upstream of the $phbC_{2.4.1}$ start codon according to heterologous expression analysis.

• Journal of Environmental Science International
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• v.18 no.2
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• pp.205-213
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• 2009

A laboratory experiment was conducted to investigate nitrogen removal from plating wastewater by a soil reactor. A combination of soil, waste oyster shell and activated sludge were used as a loading media in a soil reactor. The addition of 20% waste oyster shell and activated sludge to the soil accelerated nitrification (88.6% ${NH_4}^{+}-N$ removal efficiency) and denitrification (84.3% ${NO_3}^{-}-N$ removal) in the soil reactor, respectively. In continuous removal, the influent ${NH_4}^{+}-N$ was mostly converted to nitrate nitrogen in the nitrification soil reactor and only a small amount of ${NH_4}^{+}-N$ was found in the effluent. When methanol was added as a carbon source to the denitrification soil reactor, the average removal efficiency of ${NO_3}^{-}-N$ significantly increased. The ${NO_3}^{-}-N$ removal by methanol addition in the denitrification soil reactor was mainly due to denitrification. The phosphorus was removed by the waste oyster shell media in the nitrification soil reactor. Moreover, the phosphorus removal in the denitrification soil reactor was achieved by synthesis of bacteria and the denitrification under anaerobic conditions. The approximate number of nitrifiers and denitrifiers was $3.3{\times}10^5\;MPN/g$ soil at a depth of $1{\sim}10\;cm$ and $3.3{\times}10^6\;MPN/g$ soil at a depth of $10{\sim}20\;cm$, respectively, in the soil reactor mixed with a waste oyster shell media and activated sludge.

• Journal of the Korean Institute of Intelligent Systems
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• v.21 no.4
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• pp.499-505
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• 2011

In this paper, we proposed an adaptive fuzzy sliding mode control for nonlinear systems without parameter projection method. By modifying the controller structure, the parameters of the estimated input gain function are guaranteed not being identically zero and it is shown that the control scheme will not cause any implementation problem even if the estimated value of input gain function is zero at any moment during on-line operations. Except for the input gain function which an approximate estimate for its lower bound is needed, the proposed control scheme does not assume a priori the exact values of the bounding parameters. Based on Lyapunov synthesis methods, the overall control system guarantees that the tracking error asymptotically converges to zero and that all signals involved in controller are uniformly bounded. This can be illustrated by the simulation results for an inverted pendulum system.

• Journal of KIISE:Computer Systems and Theory
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• v.34 no.4
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• pp.158-167
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• 2007

Indirect illumination plays an important role for realistic image synthesis. We present a novel realtime indirect illumination rendering technique using image pyramids. Hundreds of thousands of indirect point light sources are stored into images, and then they hierarchically clustered into quad-tree image pyramids. We also introduce a GPU based top-down and breadth-first traversal of the quad-trees to approximate the illumination with clusters (set of lights). All steps entirely run on the GPU in real-time. Result images demonstrate that our method represents diffuse interreflection, especially a color bleeding effect well. We achieved interactive frame rates of tens to hundreads, without any preprocessing. We can avoid artifacts caused by sampling, and our method is seven times faster than a recently proposed sampling based method.

• Applied Chemistry for Engineering
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• v.27 no.2
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• pp.153-157
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• 2016

In this study, three different metal-containing ionic liquid catalysts were prepared by metal insertion and characterized by various physicochemical analytic methods. The catalytic performance of the metal containing ionic liquids in the cycloaddition of $CO_2$ with propylene oxide (PO) to produce propylene carbonate (PC) was investigated under the solvent free condition. The order of approximate rate constants ($K_{app}$) for the metal containing ionic liquid catalysts was $(MeIm)_2ZnCl_2$, > $(MeIm)_2FeCl_2$ > $(MeIm)_2CuCl_2$. These results are in accord with the experimentally obtained activity order of the different metal containing ionic liquid catalysts.

• Bulletin of the Korean Chemical Society
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• v.13 no.5
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• pp.531-537
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• 1992

The approximate rates and stoichiometry of the reaction of excess potassium 2-thexyl-1,3,2-dioxaborinane hydride(KTDBNH) with 55 selected compounds containing representative functional groups under standardized conditions (tetrahydrofuran, TEX>$0^{\circ}C$, reagent : compound=4 : 1) was examined in order to define the characteristics of the reagent for selective reductions. Benzyl alcohol and phenol evolve hydrogen immediately. However, primary, secondary and tertiary alcohols evolve hydrogen slowly, and the rate of hydrogen evolution is in order of $1^{\circ}$> $2^{\circ}$> $3^{\circ}$. n-Hexylamine is inert toward the reagent, whereas the thiols examined evolve hydrogen rapidly. Aldehydes and ketones are reduced rapidly and quantitatively to give the corresponding alcohols. Cinnamaldehyde is rapidly reduced to cinnamyl alcohol, and further reduction is slow under these conditions. The reaction with p-benzoquinone dose not show a clean reduction, but anthraquinone is cleanly reduced to 9,10-dihydro-9,10-anthracenediol. Carboxylic acids liberate hydrogen immediately, further reduction is very slow. Cyclic anhydrides slowly consume 2 equiv of hydride, corresponding to reduction to the caboxylic acid and alcohol stages. Acid chlorides, esters, and lactones are rapidly and quantitatively reduced to the corresponding carbinols. Epoxides consume 1 equiv hydride slowly. Primary amides evolve 1 equiv of hydrogen readily, but further reduction is slow. Tertiary amides are also reduced slowly. Both aliphatic and aromatic nitriles consume 1 equiv of hydride rapidly, but further hydride uptake is slow. Analysis of the reaction mixture with 2,4-dinitrophenylhydrazine yields 64% of caproaldehyde and 87% of benzaldehyde, respectively. 1-Nitropropane utilizes 2 equiv of hydride, one for hydrogen evolution and the other for reduction. Other nitrogen compounds examined are also reduced slowly. Cyclohexanone oxime undergoes slow reduction to N-cyclohexylhydroxyamine. Pyridine ring is slowly attacked. Disulfides examined are reduced readily to the correponding thiols with rapid evolution of 1 equiv hydrogen. Dimethyl sulfoxide is reduced slowly to dimethyl sulfide, whereas the reduction of diphenyl sulfone is very slow. Sulfonic acids only liberate hydrogen quantitatively without any reduction. Finally, cyclohexyl tosylate is inert to this reagent. Consequently, potassium 2-thexyl-1,3,2-dioxaborinane hydride, a monoalkyldialkoxyborohydride, shows a unique reducing characteristics. The reducing power of this reagent exists somewhere between trialkylborohydrides and trialkoxyborohydride. Therefore, the reagent should find a useful application in organic synthesis, especially in the field of selective reduction.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.59 no.1
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• pp.55-64
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• 2023

Gas turbine engines are widely used as prime movers of generator and propulsion system in warships. This study addresses the problem of designing a DS-based PID controller for speed control of the LM-2500 gas turbine engine used for propulsion in warships. To this end, we first derive a dynamic model of the LM-2500 using actual sea trail data. Next, the PRC (process reaction curve) method is used to approximate the first-order plus time delay (FOPTD) model, and the DS-based PID controller design technique is proposed according to approximation of the time delay term. The proposed controller conducts set-point tracking simulation using MATLAB (2016b), and evaluates and compares the performance index with the existing control methods. As a result of simulation at each operating point, the proposed controller showed the smallest in %OS, which means that the rpm does not change rapidly. In addition, IAE and IAC were also the smallest, showing the best result in error performance and controller effort.