• Title/Summary/Keyword: Application Procedures

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Assessing Red List categories to a Korean endangered species based on IUCN criteria - Hanabusaya asiatica (Nakai) Nakai- (멸종위기식물의 IUCN 적색목록 보전지위 평가 -금강초롱꽃에 대하여-)

  • Park, Soo-Kyung;Kim, Hui;Chang, Chin-Sung
    • Korean Journal of Plant Taxonomy
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    • v.43 no.2
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    • pp.128-138
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    • 2013
  • The conservation status of an endemic perennial herb, Hanabusaya asiatica (Nakai) Nakai (Campanulaceae) was determined by applying the IUCN risk assessment criteria from our field study and available specimen data. Also, the GIS technology was used to develop a species distribution map to calculate the extent of occurrence (EOO) and area of occupancy (AOO) for the taxon. After two years of continuous field studies, 269 mature individuals were found in four localities in 2011, while 216 mature individuals were confirmed in three localities in 2012. Based on the following data, such as EOO (2,742 $km^2$), AOO (76 $km^2$) and estimated population size of mature individuals, the taxon, which is known as 20 localities in Korean peninsula, is evaluated as the category of Endangered (EN). A major difficulty in application of IUCN criteria to Korean rare plants were the lack of essential biological information and understanding the correct knowledge of the IUCN criteria in previous Korean studies. Sound conclusions regarding the conservation status of individual species require more intensive population studies, observations, and applying IUCN assessment procedures correctly.

Application of Microwave Digestion Pretreatment Techniques for ICP-AES Analysis of Used Monolithic Automobile Catalysts Having Platinum Group-Metals and Silicates (백금족 금속과 규산염을 포함하는 모노리스형 자동차 폐촉매의 ICP-AES 분석을 위한 극초단파 분해 전처리 기법의 응용)

  • Kim, Choong-Hyon;Woo, Seong Ihl;Jeon, Sung Hwan
    • Applied Chemistry for Engineering
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    • v.10 no.4
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    • pp.568-575
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    • 1999
  • Two different digestion procedures for the simultaneous determination of major and platinum-group element(PGE) in spent automobile catalysts containing PGE and silicates by inductively coupled plasma atomic emission spectrometry(ICP-AES) are compared. Combinations of mixed aids using HF, $HNO_3$, HCl, $HClO_4$, $H_2O_2$, and $H_3BO_3$ are utilized for the hot plate dissolution method and the closed-vessel microwave digestion method, The latter method has been shown to be relatively superior in terms of recovery, analysis time, and amount of aqua regia (3 parts HCl + part $HNO_3$ required to dissolve PGE in comparison with conventional open vessel hot-plate dissolution. The best results were drawn from the following conditions: In closed Teflon PFA vessels under microwave heating with temperature/pressure regulation, a 0.25 g portion of sample was digested in 2 mL of HF, 2 mL of $HNO_3$ and 6mL of HCl under the pressure of 200 psi(13.79 bar) at $180^{\circ}C$ for 1hr, followed by a second digestion stage with 16 mL of 5%(w/v) boric acid under the pressure of 20 psi(1.38 bar) at $100^{\circ}C$ for 10 min. After the microwave heating, the sample was post-treated with 10 mL of aqua regia twice by hot-plate heating. This condition gives the PGE recovery within 85~110% and the relative standard deviations within 2%. The method developed can therefore be regarded as an alternative method for routine analysis of spent automobile catalysts.

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A Study on the Estimation of Object's Dimension based on the Vision System Model of Extended Kalman filtering (확장칼만 필터링의 비젼시스템 모델을 이용한 물체 치수 측정에 관한 연구)

  • Jang, W.S.;Ahn, H.C.;Kim, K.S.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.25 no.2
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    • pp.110-116
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    • 2005
  • It is very important to reduce the computational processing time for the application of the vision system in real time such as inspection, the determination of object's dimension and welding etc, because the vision system model involves a lot of measurement data acquired by CCD camera. Also, a lot of computation time is required in estimating the parameters in the vision system model if the iterative batch estimation method such as Newton Raphson is used. Thus, the effective computation method such as the Extended Kalman Filtering(EKF) is required to solve the above problems. The EKF has much advantages in that it takes explicitly into account the measurement uncertainties, and is a simple and efficient recursive procedures. Thus, this study is to develop the EKF algorithm to compute the parameters in the vision system model in real time. This vision system model involves the six parameters to account for the cameras inner and outer parameters. Also the EKF is applied to estimate the object's dimension. Finally, practicality of the estimation scheme of the vision system based on the EKF is verified experimently by performing the estimation of object's dimension.

Review on asbestos analysis (석면 분석방법에 대한 고찰)

  • Ham, Seung hon;Hwang, Sung Ho;Yoon, Chungsik;Park, Donguk
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.19 no.3
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    • pp.213-232
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    • 2009
  • This document was prepared to review and summarize the analytical methods for airborne and bulk asbestos. Basic principles, shortcomings and advantages for asbestos analytical instruments using phase contrast microscopy(PCM), polarized light microscopy(PLM), X-ray diffractometer (XRD), transmission electron microscopy(TEM), scanning electron microscopy(SEM) were reviewed. Both PCM and PLM are principal instrument for airborne and bulk asbestos analysis, respectively. If needed, analytical electron microscopy is employed to confirm asbestos identification. PCM is used originally for workplace airborne asbestos fiber and its application has been expanded to measure airborne fiber. Shortcoming of PCM is that it cannot differentiate true asbestos from non asbestos fiber form and its low resolution limit ($0.2{\sim}0.25{\mu}m$). The measurement of airborne asbestos fiber can be performed by EPA's Asbestos Hazard Emergency Response Act (AHERA) method, World Health Organization (WHO) method, International Standard Organization (ISO) 10312 method, Japan's Environmental Asbestos Monitoring method, and Standard method of Indoor Air Quality of Korea. The measurement of airborne asbestos fiber in workplace can be performed by National Institute for Occupational Safety and Health (NIOSH) 7400 method, NIOSH 7402 method, Occupational Safety and Health Administration (OSHA) ID-160 method, UK's Health and Safety Executive(HSE) Methods for the determination of hazardous substances (MDHS) 39/4 method and Korea Occupational Safety and Health Agency (KOSHA) CODE-A-1-2004 method of Korea. To analyze the bulk asbestos, stereo microscope (SM) and PLM is required by EPA -600/R-93/116 method. Most bulk asbestos can be identified by SM and PLM but one limitation of PLM is that it can not see very thin fiber (i.e., < $0.25{\mu}m$). Bulk asbestos analytical methods, including EPA-600/M4-82-020, EPA-600/R-93/116, OSHA ID-191, Laboratory approval program of New York were reviewed. Also, analytical methods for asbestos in soil, dust, water were briefly discussed. Analytical electron microscope, a transmission electron microscope equipped with selected area electron diffraction (SAED) and energy dispersive X-ray analyser(EDXA), has been known to be better to identify asbestiform than scanning electron microscope(SEM). Though there is no standard SEM procedures, SEM is known to be more suitable to analyze long, thin fiber and more cost-effective. Field emission scanning electron microscope (FE-SEM) imaging protocol was developed to identify asbestos fiber. Although many asbestos analytical methods are available, there is no method that can be applied to all type of samples. In order to detect asbestos with confidence, all advantages and disadvantages of each instrument and method for given sample should be considered.

SSOP Program Development for HACCP Application in Fresh Raw Fish Manufacturing (싱싱회류 생산업체의 HACCP 적용을 지원하기 위한 SSOP Program 개발과 성과)

  • 박완희;이성학;정덕화
    • Journal of Food Hygiene and Safety
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    • v.19 no.2
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    • pp.84-96
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    • 2004
  • This study aims at developing an easily-applicable Sanitation Standard Operating Procedure(SSOP) program for Fresh raw-fish manufacturing. It compares the sanitation inspection results of SSOP-program-applied procedure and non-applied procedure. The samplings for bacteriological examination were collected from the touching surface of food and from the personal workers. The sanitation status was recorded in the check-up list. The results were statistically analyzed to ascertain the efficiency of the SSOP program. The present official KFDA check-up list for sanitation inspection is composed of 51 items. Each item is judged to be fit or unfit to the standards. When the unfit items are more than 6, the work-place is judged to be insanitary. But in this study, the results of sanitation inspection are scaled in points in the 70% allotment, and the results of bacteriological examination are scaled in points in 30%, totaling 100%. Below 60 scores are judged to be insanitary and administratively punished; 61-75 scores are judged to be average; 76-85 good; above 86 excellent. The sanitation inspection scores by the improved check-up list went up by two grades from 71.8 (before SSOP program) to 88.6 (after SSOP program). The results of bacteriological examination also improved from 57.5 to 98.2. The total scores of both results converted in the allotment of 70% and 30% also showed an improvement by two grades from 66.4 (before SSOP program) to 93.4 (after SSOP program). The above results show that the manufacturers participated in this study are following the 8 essential sanitary procedures which are needed to anchor the HACCP system.

Flexural Strength Analysis of RC T-Beams Strengthened Using Fiber Sheets (섬유시트로 보강된 T형 철근콘크리트보의 휨 강도 해석)

  • Park, Tae-Hyo;Lee, Gyu-Chul
    • Journal of the Korea Concrete Institute
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    • v.15 no.2
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    • pp.234-245
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    • 2003
  • Most of the concrete bridge structures are exposed to damage due to the excessive traffic loading and the aging of the structure. The damage of concrete causes the further deterioration of the function in the concrete structure due to corrosion of the reinforced bars and decohesion between the concrete and the reinforced bar. The quick rehabilitation of the damaged concrete structures has become of great importance in the concrete structural system in order to avoid the further deterioration of the structures. Recently fiber sheets are used for strengthening the damaged concrete structures due to its many advantages such as its durability, non-corrosive nature, low weight, ease of application, cost saving, control of crack propagation, strength to thickness ratio, high tensile strength, serviceability and aesthetic. However, the lack of analytical procedures for assessing the nominal moment capacity by the fiber sheet reinforcement leads to difficulties in the effective process of decisions of the factors in the strengthening procedure. In this work, flexural strengthening effects by fiber sheets bonded on bottom face of the member are studied for the reinforced concrete T beam. In addition, auxiliary flexural strengthening effects by U-type fiber sheets bonded on bottom and side faces of the member to prevent delamination of the bottom fiber sheet are theoretically investigated. The analytical solutions are compared with experimental results of several references to verify the proposed approach. It is shown that the good agreements between the predicted results and experimental data are obtained.

A Study on the Pile Behaviour Adjacent to Tunnel Using Photo Imaging Process and Numerical Analysis (Photo Imaging Process 기법 및 수치해석을 이용한 터널주변 파일기초거동에 대한 연구)

  • Lee Yong-Joo
    • Journal of the Korean Geotechnical Society
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    • v.21 no.9
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    • pp.87-102
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    • 2005
  • In the congested urban areas, tunnelling close to existing structures or services often occurs due to the lack of surface space so that tunnelling-induced ground movements may cause a serious damage to the adjacent structures. This study focused on the two dimensional laboratory model pile-soil-tunnelling interaction tests using a close range photogrammetric technique. Testing equipments and procedures were Introduced, particularly features of aluminium rods regarded as the frictional granular material. The experimental result showed that the photo imaging process by the VMS and EngVis programs proved to be a useful tool in measuring the pile tip movements during the tunnelling. Consequently, the normalised pile tip movement data for the influence zones can be generated by the laboratory model tests using the Photogrammetric technique. This study presents influence zones associated with the normalized pile tip settlements due to tunnelling in the cohesionless material. The influence zones were Identified by both a laboratory model test and a numerical analysis. The normalized pile tip movements from the model test were in good agreement with the numerical analysis result. The influence zones proposed in this study could be used to decide the reasonable location of tunnel construction in the planning stage. However, the scale of model pile and model tunnel sizes must be carefully adjusted as real ones for practical application considering the ground conditions at a given site.

Application of HPV DNA Testing in Follow-up after Loop Electrosurgical Excision Procedures in Northern Thailand

  • Khunamornpong, Surapan;Settakorn, Jongkolnee;Sukpan, Kornkanok;Kietpeerakool, Chumnan;Tantipalakorn, Charuwan;Suprasert, Prapaporn;Siriaunkgul, Sumalee
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.14
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    • pp.6093-6097
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    • 2015
  • Background: HPV DNA testing has been recently introduced as an adjunct test to cytology in the follow-up of patients after treatment for cervical lesions using the loop electrosurgical excision procedure (LEEP). The aim of this study was to evaluate the role of HPV testing in the detection of persistent or recurrent disease after LEEP in patients with cervical epithelial lesions in northern Thailand. Materials and Methods: Patients who underwent LEEP as a treatment for histological low-grade (LSIL) or high-grade squamous intraepithelial lesion (HSIL) or worse at Chiang Mai University Hospital between June 2010 and May 2012 were included. Follow-ups were scheduled at 6-month intervals and continued for 2 years using co-testing (liquid-based cytology and Hybrid Capture 2 [HC2]) at 6 months and 24 months and liquid-based cytology alone at 12 and 18 months. Results: Of 98 patients included, the histological diagnoses for LEEP included LSIL in 16 patients, and HSIL or worse in 82 patients. The LEEP margin status was negative in 84 patients (85.7%). At follow-up, 10 patients (10.2%) had persistent/recurrent lesions; 4 among LSIL patients (25.0%) and 6 in the group with HSIL or worse (7.3%). Only 2 of 82 patients (2.4%) with HSIL or worse diagnoses had histological HSIL in the persistent/recurrent lesions. Using histologically confirmed LSIL as the threshold for the detection of persistent/recurrent disease, cytology had a higher sensitivity than HC2 (90.0% versus 70.0%). At the 6-month follow-up appointment, combined cytology and HC2 (co-testing) had a higher sensitivity in predicting persistent/recurrent disease (80.0%) compared with that of cytology alone (70.0%) and HC2 (50.0%). Conclusions: After LEEP with a negative surgical margin, the rate of persistent/recurrent lesions is low. The addition of HPV testing at the 6-month visit to the usual cytology schedule may be an effective approach in the follow-up after LEEP.

Whole Genome Association Study to Detect Single Nucleotide Polymorphisms for Body Conformation Traits in a Hanwoo Population

  • Alama, M.;Lee, Y.M.;Park, B.L.;Kim, J.H.;Lee, S.S.;Shin, H.D.;Kim, K.S.;Kim, N.S.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.3
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    • pp.322-329
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    • 2011
  • A whole genome association (WGA) study was conducted to identify quantitative trait loci (QTL) for body conformation traits in Hanwoo cattle. The phenotypes of 497 steers were recorded from the Hanwoo Improvement Center of National Agricultural Cooperative Federation, Seosan, Korea, and analyzed using the Illumina Bovine 50 k SNP chip. A set of 35,987 SNPs that were available in the Hanwoo population was selected from the chip. After adjustments for the effects of year-season of birth, region and sire, phenotypes were regressed on each SNP using a linear regression model. Three hundred nineteen SNPs were detected for the ten conformation traits (p<0.003). For the significant SNPs, stepwise regression procedures were applied to determine best sets of markers. A total of 72 SNPs were selected (p<0.001), for which the sets of 5, 9, 10, 9, 8, 11, 4, 6, 3 and 7 SNPs were determined for height at withers, rump height, body length, chest depth, chest width, rump length, hip width, thurl width, pinbone width and heart girth, respectively. About 7-26% of the total phenotypic variation was explained by the set of SNPs for each trait. QTL for the conformation traits were harbored on most bovine chromosomes (BTAs). Four SNPs with pleiotropic effects on height at withers and rump height were detected on BTAs 3, 4, 6 and 16. A SNP with pleiotropic effects on chest width and rump length was also detected on BTA10. Two QTL regions, i.e. between 87 and 97 Mb in BTA3 and between 41 and 44 Mb in BTA7, were found, in which SNPs were detected for the five and three conformation traits, respectively. The detected SNPs need to be validated in other Hanwoo populations for commercial application to the genetic improvement of conformation characteristics in Hanwoo via marker-assisted selection (MAS).

Estimating Cumulative Distribution Functions with Maximum Likelihood to Sample Data Sets of a Sea Floater Model (해상 부유체 모델의 표본 데이터에 대해서 최대우도를 갖는 누적분포함수 추정)

  • Yim, Jeong-Bin;Yang, Won-Jae
    • Journal of Navigation and Port Research
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    • v.37 no.5
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    • pp.453-461
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    • 2013
  • This paper describes evaluation procedures and experimental results for the estimation of Cumulative Distribution Functions (CDF) giving best-fit to the sample data in the Probability based risk Evaluation Techniques (PET) which is to assess the risks of a small-sized sea floater. The CDF in the PET is to provide the reference values of risk acceptance criteria which are to evaluate the risk level of the floater and, it can be estimated from sample data sets of motion response functions such as Roll, Pitch and Heave in the floater model. Using Maximum Likelihood Estimates and with the eight kinds of regulated distribution functions, the evaluation tests for the CDF having maximum likelihood to the sample data are carried out in this work. Throughout goodness-of-fit tests to the distribution functions, it is shown that the Beta distribution is best-fit to the Roll and Pitch sample data with smallest averaged probability errors $\bar{\delta}(0{\leq}\bar{\delta}{\leq}1.0)$ of 0.024 and 0.022, respectively and, Gamma distribution is best-fit to the Heave sample data with smallest $\bar{\delta}$ of 0.027. The proposed method in this paper can be expected to adopt in various application areas estimating best-fit distributions to the sample data.