• Korean Journal of Clinical Laboratory Science
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• v.51 no.4
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• pp.475-483
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• 2019

The use of stem cells in cell-based therapy has attracted extensive interest in the field of regenerative medicine, and it has been applied to numerous incurable diseases due to the inherent abilities of self-renewal and differentiation. However, there still exist some severe obstacles, such as requirement of cell expansion before the treatment, and low survival at the treated site. To overcome these disadvantages of stem cells, we used the carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM 6) gene, which functions to increase cell-cell interaction as well as anti-apoptosis. We first confirmed whether CEACAM 6 is expressed in various cell lines at the protein level (including in stem cells), followed by evaluating and selecting the optimal transfection conditions into stem cells. The CEACAM 6 gene was transfected into stem cells to prolong cell survival and preserve from damage by oxidative stress. After confirming the CEACAM 6 expression in transfected stem cells, the cell survival was assessed under oxidative condition by exposing to hydrogen peroxide (H₂O₂) to mimic the chronic environment-induced cellular damage. CEACAM 6 expressing stem cells show increased cell viability compared to the non-CEACAM 6 expressing cells. We propose that the application of the CEACAM 6 gene is a potential option, capable of expanding and enhancing the therapeutic effects of stem cells.

• Resources Recycling
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• v.13 no.4
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• pp.23-31
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• 2004

Basic studies have been conducted regarding the attempt of the utilization of waste Undaria pinnatifida as an adsorbent for the adsorption treatment of lead-containing wastewater. Undaria pinnatifida was found to be chiefly composed of hyo-carbonaceous compounds and have a fairly high specific surface area, which suggesting the possibility of its application as a Potential adsorbent. The electrokinetic Potential of Undaria pinnatifida particles was observed to be negatively highest at around pH 8 and the fact that its electrokinetic potentials are negative at the whole pH range supported it might be an efficient adsorbent especially for cationic adsorbates. Under the experimental conditions, $Pb^{2+}$ was found to mostly adsorb onto Undaria pinnatifida within a few minutes and reach the equilibrium in adsorption within ca. 30 minutes. The adsorption of $Pb^{2+}$ was exothermic and explained well by e Freundlich model. Acidic pretreatment of Undaria pinnatifida enhanced its adsorption capacity for $Pb^{2+}$ , however, the reverse was observed for alkaline pretreatment. The formation of organometallic complex between $Pb^{2+}$ and some functional groups on the surface of Undaria pinnatifida was considered to be one of the main drives for adsorption. Finally the adsorbability of$ Pb^{2+}$ was examined to be rather affected by several solution features such as the coexistence of other adsorbate, the variation of ionic strength, and the concentration of complexing agent.

• Journal of Korean Tunnelling and Underground Space Association
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• v.22 no.3
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• pp.277-291
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• 2020

Shield TBM is a tunnelling method that has a wider range of applications in the poor ground condition compared to conventional tunnels (Drill and Blast). Currently, a 13.3 m large-diameter slurry shield TBM is preparing for construction to pass under the Han River. Shield TBM is divided into slurry and EPB shield TBM, and management items during construction are different depending on each characteristic. In this paper, the equipment type, origin, application case and trouble case were analyzed for slurry shield TBM, which is mainly constructed in soft ground. In addition, 2D and 3D model tests were conducted on the condition of soil depth for the possibility of slurry leakage into front of the equipment, with appropriate chamber pressure. Based on this paper, it proposed to provide basic and reference data for proper excavation surface pressure and chamber pressure during construction of slurry shield TBM under soft ground conditions, and proposed measures to minimize stability and environmental decline due to slurry ejection.

• Journal of Legislation Research
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• no.44
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• pp.387-433
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• 2013

Understanding the concepts of health and medical practice are significant because they are the basis of health and medical policy and law. How legislators, policy makers, and the public perceive those concepts defines the direction of legislation and policy making. This study aims to show the changes of the concepts by reviewing the history of major Statutes relating to health and medicine. Alongside medicalization of human conditions and daily activities, the concepts of health and medical practice also expanded. On the other hand, as technologies of health and medicine have specialized and segmented, the large portion of public services of the past is now provided by the private sector. We argue that the actual laws and decisions by the judiciary should be responsive to social and scientific changes, which may cause the changes of the perceptions of health and medical practice. By doing so, they not only can have actual legal force but also even initiate a movement for establishing new medical policy or law.

• Ceramist
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• v.21 no.4
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• pp.378-387
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• 2018

The optimal fabrication conditions for $Gd_{0.1}Ce_{0.9}O_{2-{\delta}}$(GDC) buffer layer and $La_{0.6}Sr_{0.4}Co_{0.2}Fe_{0.8}O_{3-{\delta}}$ (LSCF) cathode on 1mol% $CeO_2-10mol%\;Sc_2O_3$ stabilized $ZrO_2$ (CeScSZ) electrolyte were investigated for application of IT-SOFCs. GDC buffer layer was used in order to prevent undesired chemical reactions between LSCF and CeScSZ. These experiments were carried out with $5{\times}5cm^2$ anode supported unit cells to investigate the tendencies of electrochemical performance, Microstructure development and interface reaction between LSCF/GDC/CeScSZ along with the variations of GDC buffer layer thickness, sintering temperatures of GDC and LSCF were checked, respectively. Electrochemical performance was analyzed by DC current-voltage measurement and AC impedance spectroscopy. Microstructure and interface reaction were investigated by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). Although the interfacial reaction between these materials could not be perfectly inhibited, We found that the cell, in which $6{\mu}m$ GDC interlayer sintered at $1200^{\circ}C$ and LSCF sintered at $1000^{\circ}C$ were applied, showed good interfacial adhesions and effective suppression of Sr, thereby resulting in fairly good performance with power density of $0.71W/cm^2$ at $800^{\circ}C$ and 0.7V.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.294-294
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• 2019

The system for predicting flood of river at Flood Control Office is made up of a rainfall-runoff model and FLDWAV model. This system is mainly operating to predict the excess of the flood watch or warning level at flood forecast points. As the demand for information of the management and operation of riverside, which is being used as a waterfront area such as parks, camping sites, and bike paths, high-level forecasts of watch and warning at certain points are required as well as production of lowland flood forecast information that is used as a waterfront within the river. In this study, a technology to produce flood forecast information in lowland areas of the river used as a waterfront was developed. Based on the results of the 1D hydraulic analysis, a model for performing spatial operations based on high resolution grid was constructed. A model was constructed for Andong district, and the inundation conditions and level were analyzed through a virtual outflow scenarios of Andong and Imha Dam.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.5
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• pp.569-577
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• 2019

Urban flooding has been a frequent phenomenon in recent years caused by the increase in maximum stormwater runoff arising from abnormal rainfall due to global warming, urban development, and development of lowlands according to population inflows. In order to respond positively against abnormal precipition in the city, it is necessary to check the GWI (Green Water Infra) effect and effectively utilize the existing stormwater detention tanks and treat stormwater to prevent local flooding. In this study, Overflow Type stormwater drainage methods are evaluated as a method of preventing urban flooding in abnormal precipitation using the Dynamic Wave Analysis SWMM (Storm Water Management Model) provided by the United States Environmental Protection Agency. Comparing and analyzing the Upward Watergate Type and Overflow Type, it was analyzed that the Overflow Type reduces the maximum flood discharge by 61 % and the total flood volume by 56 % in the rainfall of Typhoon Kong-rey. The application of the Overflow Type and the natural-pneumatic drainage method to the rainfall of Typhoon Soulik resulted in a 20 % reduction in maximum flood runoff and a 67 % reduction in total flood quantity. Therefore, as a solution to the abnormal rain fall, it is possible to improve the existing stormwater detection tank and install additional facilities. It is expected to be economically possible to strom drainage under limited conditions.

• Polymer(Korea)
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• v.33 no.2
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• pp.104-110
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• 2009

Demineralized boneparticle (DBP) has been widely used as and a powerful promoter of new bone growth. In this study, DBP sponges were chemically crosslinked and characterized for the potential application of tissue engineered scaffolds. The DBP sponges prepared by crosslinking with EDC. 0.1, 0.2 or 0.3% pepsin was applied to DBP dissolved in 3% (v/v) acetic acid aqueous solution for 48 hrs. The prepared sponges were crosslinked by 1, 5, 10, 50 or 100 mM of EDC solution concentration and then were lyophilized. The DBP sponges were characterized by SEM, FT-IR and DSC and analyzed in terms of their porosity and water absorption ability. The cellular viability and proliferation were assayed by MTT assay. Our investigation revealed that 0.2$\sim$0.3% of pepsin and 50$\sim$100 mM of EDC produced DBP sponges with good physical characteristics. In conclusion, DBP sponge prepared under these conditions is potentially useful for the applications of tissue construction.

• Food Science and Preservation
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• v.6 no.4
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• pp.351-356
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• 1999

To develop and extend the shelf-life of vitamin C enriched fresh-cut apples using CA stored Fuji apples, dipping in 10% L-ascorbic acid and packaging with 0.03 mm LDPE, 0.04 mm PP and 0.08 mm Nylon/PE film(N$_2$displacement) were carried out. The changes of gas concentrations in the packaging and quality attributes of fresh-cut apples were examined during storage at 10$^{\circ}C$. The concentrations of O$_2$was maintained lower in Nylon/PE film than the other film, the level of O$_2$was in the range of 1∼3%. The increase of C$_2$H$_4$ concentrations in Nylon/PE film bag was more suppressed than the others. The vitamin C content of fresh-cut apples was enriched by dipping in L-ascorbic acid solution up to 241 mg$.$100 g-1 f.w., and the loss or that content was retarded differently by the package conditions of lower O$_2$level during storage. Browning in fresh-cut apples was shown after 6 days of storage in LDPE and PP film. but it was not shown by 14 days of storage in Nylon/PE film. Spoilage and off odor in fresh-cut apples were not detected up to 14 days of storage in Nylon/PE film. The results indicated that the vitamin C enriched fresh-cut apples can be processed from the long-term CA stored apples, and maintaining high quality of the products ill be possible in cases of the application of sealing packaging after O$_2$removal with film having low O$_2$permeability.

• Journal of Fisheries and Marine Sciences Education
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• v.11 no.1
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• pp.69-87
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• 1999

This study had been analysed the establishment, the closing and the actual conditions of the fisheries school according to the four times revision and promulgation of the law of education of korea which were devided by the early term (the first Chosun educational decree), the middle term (the second Chosun educational decree) and the last term (the third and fourth Chosun educational decree), and also been investigated how the fisheries education of the school had been acted to the mobocracy and the assimilation policy and together with it's back ground through this analysis. The aim of this study is to contribute to the study of the history of the fisheries school education, analysis of change of the fisheries school education according to practical application of the Chosun educational decree. The summary of the characteristic of the each term are as under. First, in the early term of the fisheries education under the first Chosun educational decree, Experts were despatched to the each province with donated money from emperor and opened seasonal the fishing training centre, a kind of social fisheries education, and trained directly fishing technic and were going to train fisheries trainees regularly under name of elementary fisheries school. Japanese imperialism attached great importance to the vocational education in order to snack economical products from colonized Korea but actually had a purpose to train low quality technician who follow blindly their colony policy of Japanese imperialism. The fisheries schools in the circle of system in early time of Japanese imperialism, Kunsan public elementary fisheries school was established in April 1915, Yosu public elementary fisheries school was established ill May 1917 and Kyungnam Tongyoung training school was established in March 1917. Secondly, After 3.1 movement, the Japanese imperialism established an appeasement policy so called cultural politics and continued assimilation policy with skilful methods. After revision of the second Chosun educational decree, the Elementary vocational school was changed as the vocational training school. The school of fisheries education in middle of Japanese imperialism trained low quality technicians to snack fisheries resources from colonized Korea. After the middle of Japanese imperialism they paid attention on training fisheries technician through fisheries school rather than training school. With high interest and crowded volunteers, Kunsan public fisheries school was promoted in 1922, Tongyoung public fisheries school was promoted in 1923, Yongampo fisheries training school established in 1922 was promoted as Yongampo public fisheries school in 1926. Thirdly, in the time of the third and fourth Chosun educational decree, the end of Japanese imperialism, they met Pacific war after Japan vs China war. During the war time they considered the vocational school as the source of supply for materials and manpower and consequently had to expanded vocational education and systematically despatched students to war field and practiced military training. In 1938, Namhae public fisheries school was established and Chungjin fisheries school was permitted. But in order to supply manpower to Pacific war, the study period of Yosu public fisheries school was shorten from 5 years to 4 year in 1943 and also that of Tongyoung public fisheries school shorten in 1944.