• 한국어병학회지
• /
• 제17권2호
• /
• pp.123-130
• /
• 2004

꿀벌 (Apis mellifera)의 lymph 액으로부터 정제된 항균성 펩타이드인, Apidaecin Ⅰb는 18개의 아미노산 잔기로 구성된 염기성 non-helical 펩타이드이다. 본 연구에서는 우리나라 연안의 적조 (HABs, harmful algal blooms)를 일으키는 4종의 적조생물 (Alexandrium tamarense, Chattonella marina, Cochlodinium polykrikoides 및 Gymnodinium catenatum)에 대한 Apidaecin Ⅰb의 살조효과를 조사하였다. Apidaecin Ⅰb의 4종의 적조생물에 대한 살조효과는 12.5$\mu{g}/mL$부터 50$\mu{g}/mL$에서 세포의 lysis 또는 ecdysis와 같은 형태로 현미경으로 관찰할 수 있었다. 또한 Apidaecin Ⅰb는 C. marina에 대해서 A. tamarense, C. polykrikorides 및 G. catenatum보다 더욱 강한 살조효과를 나타내었다. 이러한 HABs에 대한 Apidaecin Ⅰb의 살조효과 연구는 새로운 살조물질을 개발하기 위한 자료가 될 것으로 생각된다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제24권1호
• /
• pp.37-40
• /
• 2012

The apidaecins are highly active against Gram-negative bacteria. Here, we show the expression and antibacterial activity of the bumblebee, Bombus ignitus, apidaecin. We PCR-amplified 51 bp of the active domain sequence of the B. ignitus apidaecin gene and expressed the recombinant B. ignitus apidaecin active domain in baculovirus-infected insect cells. The recombinant B. ignitus apidaecin active domain shows bactericidal activity against Gram-negative bacteria, including Pseudomonas tolaasii, a serious pathogen in cultivated mushrooms, but not Gram-positive bacteria. This result suggests that the active domain of the B. ignitus apidaecin is a potential antibacterial agent for the control of bacterial brown blotch diseases.

• Bulletin of the Korean Chemical Society
• /
• 제33권1호
• /
• pp.115-122
• /
• 2012

Apidaecin Ib had strong antimicrobial activity against several tested Gram-negative bacteria including Escherichia coli, Enterobacter cloacae, and Shigella flexneri (MECs; $0.3-1.5{\mu}g/mL$), but showed no activity against all the tested Gram-positive bacteria including Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus and one yeast, Candida albicans (MECs; > $125{\mu}g/mL$). Interestingly, this peptide showed potent antibacterial activity only against Edwardsiella species (MECs; $0.6-3.6{\mu}g/mL$) among the tested fish pathogenic bacteria through a bacteriostatic process and showed no significant hemolytic activity. Apidaecin Ib took an unordered structure in all environments and also had very weak membrane perturbation activity even at $25{\mu}M$. Anti-Edwardsiella activity of apidaecin Ib is stronger than those of other antimicrobial polypeptides or antibiotics, but its activity is salt-sensitive. These results suggest that apidaecin Ib has Edwardsiella speciesspecific antibacterial activity and could be applied as new preventive or control additives for Edwardsiella species infection in freshwater fish aquaculture.

• Bulletin of the Korean Chemical Society
• /
• 제31권1호
• /
• pp.209-212
• /
• 2010