• 제목/요약/키워드: Apidae

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Contribution to Taxonomy and Distribution of the Genus Elaphropoda (Hymenoptera: Apidae: Apinae) in Vietnam

  • Nguyen, Minh Phuong;Tran, Ngat Thi;Nguyen, Dai Dac;Nguyen, Lien Thi Phuong
    • Animal Systematics, Evolution and Diversity
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    • 제32권2호
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    • pp.118-122
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    • 2016
  • Taxonomic notes on the genus Elaphropoda Lieftinck, 1966 (Hymenoptera: Apidae) from Vietnam are presented. Two species of the genus are reported: Elaphropoda percarinata (Cockerell, 1930) was first recorded from Vietnam based on specimens collected from Ha Tinh province in 1998, and is reconfirmed with a specimens collected from Bac Kan province in the Northeastern part of the country in this study, and Elaphropoda khasiana (Schulz, 1906) is recorded from Vietnam for the first time. Redescriptions of the male of E. percarinata and the female of E. khasiana are given with illustrations.

Phylogenetic Relationships among Some Bumblebees (Hymenoptera: Apidae) Common in Korea Inferred from Mitochondrial 16S rRNA Sequences

  • Yoon, Hyung-Joo;Lee, Myeong-Lyeol;Kim, Sam-Eun;Lee, Sang-Beom;Kim, Iksoo;Bae, Jin-Sik;Jin, Byung-Rae;Sohn, Hung-Dae
    • International Journal of Industrial Entomology and Biomaterials
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    • 제8권1호
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    • pp.51-59
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    • 2004
  • Phylogenetic relationships were analyzed among bumblebees using a portion of mitochondrial (mt) 16S ribosomal RNA (16S rRNA). Eight species of true bumblebees and one species of cuckoo bumblebee (Bombini, Apidae), collected from Korea were included in the analysis. Also, one species of true bumblebee imported from several foreign countries for pollination was included. The length of mt 16S rRNA sequence ranged from 496 bp to 508 bp and sequence divergence ranged from 1.4% (7 bp) to 15.49% (77bp). As expected, a high A+T content was observed (78.5% on average). According to the phylogeny tree derived from parsimony and maximum likelihood analysis, a monphyletic Bombus species, excluding a single cuckoo bumblebee, Psithyrus coreanus, was obtained, but the bootstrap estimate at the node supporting the monophyletic group was very weak (40% or 46%), suggesting a very close relationship of the cuckoo bumblebee to the true bumblebee. Within Bombus species belonging to identical subgenera subgeneric specific clustering was formed with high bootstrap values, implying validity of the subgeneric names of each species: Pyrobombus for B. ardens and B. modeatus; Megabombus for B. consobrinus wittenburgi and B. koreanus; and Bombus s. str. for B. ignitus, B. hypocrita sapporoensis, and B. terrestris.

Population Genetic Structure of the Bumblebee, Bombus ignitus (Hymenoptera: Apidae), Based on Mitochondrial COI Gene and Nuclear Ribosomal ITS2 Sequences

  • Oh, Hyung Keun;Yoon, Hyung Joo;Lee, Joo Young;Park, Jeong Sun;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • 제27권1호
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    • pp.142-158
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    • 2013
  • The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is widely utilized for greenhouse pollination in South Korea. Understanding the magnitude of genetic diversity and geographic relationships is of fundamental importance for long term preservation and utilization. As a first step, we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to the "DNA barcode" region and the complete internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA from 88 individuals collected in nine South Korean localities. The complete ITS2 sequences were longest among known insects, ranging in size from 2,034 bp ~ 2,052 bp, harboring two duplicated 112-bp long repeats. The 658-bp long mtDNA sequences provided only six haplotypes with a maximum sequence divergence of 0.61% (4 bp), whereas the ITS sequences provided 84 sequence types with a maximum sequence divergence of 1.02% (21 sites). The combination of the current COI data with those of published data suggest that the B. ignitus in South Korea and China are genetically a large group, but those in Japan can be roughly separated into another group. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population were found to exist among the South Korean populations of B. ignitus, which suggests panmixia. This finding is consistent with our understanding of the dispersal capability of the species.

Intron sequence diversity of the asian cavity-nesting honey bee, Apis cerana (Hymenoptera: Apidae)

  • Wang, Ah Rha;Jeong, Su Yeon;Jeong, Jun Seong;Kim, Seong Ryul;Choi, Yong Soo;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • 제31권2호
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    • pp.62-69
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    • 2015
  • The Asian cavity-nesting honeybee, Apis cerana (Hymenoptera: Apidae), has been extensively studied for its biogeography and genetic diversity, but the molecules utilized in past studies were mainly ~90 bp long mitochondrial non-coding sequences, located between $tRNA^{Leu}$ and COII. Thus, additional molecular markers may enrich our understanding of the biogeography and genetic diversity of this valuable bee species. In this study, we reviewed the public genome database to find introns of cDNA sequences, with the assumption that these introns may have less evolutionary constraints. The six introns selected were subjected to preliminary tests. Thereafter, two introns, titled White gene and MRJP9 gene, were selected. Sequencing of 552 clones from 184 individual bees showed a total of 222 and 141 sequence types in the White gene and MRJP9 gene introns, respectively. The sequence divergence ranged from 0.6% to 7.9% and from 0.26% to 17.6% in the White gene and the MRJP9 introns, respectively, indicating higher sequence divergence in both introns. Analysis of population genetic diversity for 16 populations originating from Korea, China, Vietnam, and Thailand shows that nucleotide diversity (π) ranges from 0.003117 to 0.025837 and from 0.016541 to 0.052468 in the White gene and MRJP9 introns, respectively. The highest π was found in a Vietnamese population for both intron sequences, whereas the nine Korean populations showed moderate to low sequence divergence. Considering the variability and diversity, these intron sequences can be useful as non-mitochondrial DNA-based molecular markers for future studies of population genetics.

Phylogenetic Relationship among the Bumblebees (Hymenoptera: Apidae) Common in Korea Inferred from Mitochondrial 16S rRNA Sequences

  • Yoon, Hyung-Joo;Lee, Myeong-Lyeol;Kim, Sam-Eun;Bae, Jin-Sik;Kim, Iksoo;Lee, Sang-Beom;Kim, Keun-Yong;Jin, Byung-Rae;Sohn, Hung-Dae
    • 한국잠사학회:학술대회논문집
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    • 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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    • pp.123-125
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    • 2003
  • We provide a molecular study intending to derive an estimate of the relationships within the genus Bombus, 10 species which span 6 subgenera common collected from several regions of Korea and B. terrestris imported from several foreign countries for pollination using a portion of mitochondrial 16S ribosomal RNA. (omitted)

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