• Food Science and Preservation
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• v.7 no.1
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• pp.113-116
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• 2000

The antimicrobial activities of three parts of Korean sword bean , such as whole seed, dotyledon and hull were investigated . Yields were higher hull other parts in water and methanol extract. Methanol extract in seed parts was fractionated with different solvents, such as hexane, chloroform, ethyl acetate, butanol and water, yields of water fractino showed 3.3% , 2.5% and 0.3% in whole seed, 채쇼ledon and hull, respectively. In solvent extracts using methanol, hexane, chloroform, and water , methanol extract showed the most effective antimicrobial activities. Antimicrobial activities of ethyl acetate fraction of methanol extractwas higher than those of other fractions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.4
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• pp.574-584
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• 1998

Antimicrobial activities, extraction yields and chemical compositions of the extract from the powder of vacuum dried Smilax china root on various solvents were investigated. Antimicrobial activities against. A rhizogenes, A. tumefaciens, C. utilis, S. cerevisiae, B. megaterium, B. subtilis and E. coli were measured by disc diffusion method. Methanol, chloroform, ethylacetate and butanol extracts exhibited potent antimicrobial activities against B. megaterium and B. subtilis. Also, methanol, ethylacetate and butanol extracts had the activities against A. rhizogenes, and chloroform and ethylacetate extracts against A. tumefacciens. The extraction yields were 13.10%, 8.71%, 2.73%, 0.83%, 0.54% and chemical compositions of their extracts were very different according to the kinds of solvents. The main chemical groups of ethylacetate, methanol, chloroform and butanol extracts were 45.99% phenolics, 36.10% acids, 29.13% phenolics and 18.96% phenolics, respectively.

• YAKHAK HOEJI
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• v.18 no.4
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• pp.243-248
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• 1974

Nine benzoxazolin-2-thione derivatives were sunthesized as the potential antimicrobial substances. These compounds were tested for the antimicrobial activities using Staphylococcus aureus and Escherichia coli by way of tube dilution method. The three compounds of I, II and VII omjoboted the growth of S.aureus at the concentration of 1${\mu}$g/ml, and III,VI,VIII and IX exhibited considerable antimicrobial activities against S. aureus at the concentration of 10.m.u.g/ml. As to the growth of E. Coli, VII VII and VIII inhibited at the concentration of 1${\mu}$g/,l. II,III and IV exhibited considerable antimicrobial activities against E. coli at the concentration of 10${\mu}$g/ml.

• The Korea Journal of Herbology
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• v.32 no.1
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• pp.75-81
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• 2017

Objectives : The present study was designed to investigate effects of mixed extracts (9:1, v/v) of Hwangryunhaedoktang, Chamaecyparis obtusa essential oil. We evaluated the antimicrobial and antioxidant activity by manufacturing mixed extracts as the materials for functional medicinal herb cosmetics. Methods : We performed antimicrobial were tested microbes (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans, Propionibacterium acnes) by disc diffusion method and measure clear zone. Antioxidant activities were measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. Results : Antimicrobial activities of mixed extracts against S. epidermideis, P. acnes, S. aureus, E. coli, and Candida. A were $10.9{\pm}3.0mm$, $16.9{\pm}2.0mm$, $9.2{\pm}2.0mm$, $10.3{\pm}1.0mm$, $11.8{\pm}3.0mm$ respectively. The Chamaecyparis obtusa essential oil had the highest antimicrobial activities against S. epidermideis, P. acnes, S. aureus, E. coli, and Candia. A and clear zone of microbes ware $16.7{\pm}3.0mm$, $28.8{\pm}0.2mm$, $15.9{\pm}2.0mm$, $11.5{\pm}1.0mm$, $16.3{\pm}3.0mm$. Hwangryunhaedoktang extract showed antimicrobial activity but only P. acnes and S. aureus The antioxidant activities of the mixed extracts were tested through the evaluation of DPPH radical scavenging activity. The 100 % mixed extracts were found to have 90 % DPPH radical scavenging activity. The mixed extracts was presented similar antioxidant activities compared with that of ascorbic acid. As a result, A mixture extract is expected to have antimicrobial effects and free radical scavenging activity was found. Conclusions : Accordingly, It can be concluded that mixed extracts has the potential to cosmetic material.

• Archives of Pharmacal Research
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• v.25 no.6
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• pp.860-864
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• 2002

In this study the composition and antimicrobial properties of essential oils obtained from Origanum onites, Mentha piperita, Juniperus exalsa, Chrysanthemum indicum, Lavandula hybrida, Rosa damascena, Echinophora tenuifolia, Foeniculum vulgare were examined. To evaluate the in vitro antibacterial activities of these eight aromatic extracts; their in vitro antimicrobial activities were determined by disk diffusion testing, according to the NCCLS criteria. Escherichia coli (ATTC 25922), Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATTC 27853 were used as standard test bacterial strains. Origanum onites recorded antimicrobial activity against all test bacteria, and was strongest against Staphylococcus aureus. For Rosa damascena, Mentha piperita and Lavandula hybrida antimicrobial activity was recorded only to Staphylococcus aureus. Juniperus exalsa, and Chrysanthemum indicum exhibited antibacterial activities against both Staphylococcus aureus and Escherichia coli. We also examined the in vitro artimicrobial activities of some components of the essential oils and found some components with antimicrobial activity.

• Journal of Environmental Health Sciences
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• v.37 no.2
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• pp.133-140
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• 2011

Antimicrobial activity and bactericidal activity of Caesalpinia sappan L. extracts were investigated against five food-borne pathogens, E. coli, S. aureus, S. typhimurium, B. cereus and L. monocytogenes. Methanol extract of Caesalpinia sappan L. revealed antimicrobial activities against five pathogens. In particular, by paper disc diffusion the highest activity was shown against L. monocytogenes. Antimicrobial activities of methanol extracts showed the most potent activities, but hexane fraction had no activity. Fractions of ethyl acetate and butanol turned out to have higher antimicrobial activities against Gram(+) bacteria than Gram(-) bacteria. The minimum inhibitory concentration against five food-borne pathogens was 1.563 mg/ml on Gram(+) bacteria and 3.125 mg/ml on Gram(-) bacteria. The result of antimicrobial activity in a shaking flask method showed that bacterial growth rate fell by more than 99.999% at 3.125 mg/ml of methanol extract. The highest rate of viable reduction (99.998%) was shown at 0.781 mg/ml of methanol extract against L. monocytogenes. After five minutes of reaction between test strains and methanol extracts, the growth rates of five kinds of bacteria were reduced by more than 99.999% at a concentration of 100 mg/ml. Therefore, it is suggested that methanol extracts of Caesalpinia sappan L. can be developed as a natural sanitizer or disinfectant.

• Microbiology and Biotechnology Letters
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• v.32 no.4
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• pp.352-355
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• 2004

The extracts from 70 different plant natural products were used to investigate the antimicrobial activities against Brachyspira hyodrsenteriae, the causative agent of swine dysentery. Among them, the extracts from Terminalia chehula exhibited the highest antimicrobial activities against B. hyodysenteriae. It showed the antimicrobial activity against B. hyodysensteriae mostly in parts of its shell. Minimum inhibitory concentration (MIC) of the water extracts of T chehula was 12.5 mg/ml. After organic solvent extractions, the ethylacetate extracts exhibited higher antimicrobial activities against B. hyodysenteriae, and their antimicroactivities were similar to that of the tannin. In conclusion, these findings suggest that the extracts from T chebula may be used as animal feed additives for their antimicrobial activities against B. hyodysenteriae.

• Archives of Pharmacal Research
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• v.8 no.1
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• pp.21-30
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• 1985

Examination of the constituents of a marine sponge, Aplysina fishtularis, indicated that brominated tyrosine metabolites were mainly responsible for antimicrobial and antineoplastic activities. Halogenated tyrosine metabolites, 2, 6-dibromo-(2), 2-bromo-3-chloro-(3) and 2, 6-dibromo-(5), 5-a mino-2-bromo-6-chloro-(6) and 5-amino-2, 6-dich-loro-(7) 4-hydroxy-2-cyclohexenone-4-acetic acid lactams were identified as the major antineoplastic and antimicrobial principles. Many other brominated tyrosine metabolites were also confirmed, but they did not show antimicrobial and antineoplastic activities.

• Biomedical Science Letters
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• v.14 no.1
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• pp.1-6
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• 2008

This investigation was performed to study the antioxidant activities and the antimicrobial effect of plum (Santarosa, Oishiwase) extracts. Plums were extracted by ultrasound-assisted method and boiling method. All extracts of plums showed concentration-dependent inhibitory effects on the DPPH free radical scavenging activity. In the superoxide anion radical scavenging method, all the plum extracts showed lower activity than BHT. But in case of sonicate extract of Oishwase exhibited the highest activity in plum extracts. The antimicrobial effect of plums used for human skin- or oral cavity-presented strains; Bacillus cereus (KCTC 1012) and Staphylococcus aureus (KCTC 1927). Addition of plum extracts was used by autoclaved and filtrated. Each extract solution was added into culture media with several concentration and then the bacteria cell growth was investigated for 72 hours. The effect of antimicrobial activities showed in a higher Staphylococcus aureus than Bacillus cereus. Results indicate that the autoclaved sample showed a higher antimicrobial activity than did the filtrated sample.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.2
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• pp.232-238
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• 1998

To developed natural food preservatives, ethanol and water extracts were prepared from the defatted camellia(Camellia japonica L.) seeds. Antimicrobial activities were examined against 14 microorganisms which were food borne pathogens and/or food poisoning microorganisms and food-related bacteria and yeasts. Ethanol extract showed antimicrobial activities against several microorganisms tested, but not on lactic acid bactria. Especially, minimum inhibitory concentrations(MIC) for yeasts were as low as 1mg/ml. Water extract exhibited antimicrobial activities for the yeasts tested, but almost bacteria were not observed. The ethanol extract was fractionated in the order of hexane, chloroform, ethylaetate and butanol fractions to test antimicrobial activity. The strongest activity for the tested yeasts were found in the butanol fractions, but bacteria were chloroform fraction. Hansenula anomala treated with ethanol were observed by scanning electron micrograph, and the results exhibited morphological changes, including the irregularly contracted cell surface and expanded ellipsoidal shape.