• 한국가축번식학회지
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• 제23권1호
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• pp.63-68
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• 1999

선천성 전신수종과 함께 체간의 형성부전을 특징으로 하는 한우 기형 송아지를 해부학적 및 혈청학적으로 검사하여 다음과 같은 결과를 얻었다. 기형의 송아지는 정상 송아지의 2/3 정도 크기 (체장 82cm, 무게 25kg) 이었다. 태자는 외관상 목과 몸통의 구분이 어려웠고 두부에서 포는 보이지 않았으며, 목에 해당하는 부분에 혓바닥을 물고 있는 입이 있었다. 사지의 형태는 유지하고 있었으나 체간의 형성부전으로 기형을 나타내었고 얼굴을 비롯 몸통 전체의 피하에 심한 수종에 의해 파동성을 보였다. 부검시 흉강과 복강내에 맑은 장액성 액상물질이 가득 차 있었고 피하조직내에도 같은 물질의 저류로 인해 피부와 피하의 구별이 되지 않았다. 더불어 이들 수종성 피하에는 연황색의 과립양 물질들이 다수 혼재되어 있었다. 내부장기는 거의 식별하기 어려웠고 흉강과 복강이 부분적으로 개통되어 간의 일부가 흉강쪽으로 빠져 나와 있었다. 폐는 크기가 32$\times$49 mm로 심장(56$\times$45 mm) 보다도 더 작게 위축되어 있었다. 복강에서는 두 개의 수종성 좌측 신장 (15$\times$21cm, 13$\times$18cm)과 1 개의 우측신장 (13$\times$9cm) 이 있었고 이들의 할단면에서의 실질부위는 대부분이 낭포로 채워진 낭종성 기형을 나타내었다. 간은 담황색 변성과 종대를 보였고, 비장은 부종, 충혈 및 종대가 관찰되었으며, 부종성 소장과 대장은 장관의 형태는 유지하고 있었으나 장간막에 황색 결절이 미만성으로 형성되어 있었다. 한편, 이 기형 송아지의 발생 원인체 중 하나로 모우에서 64 배 이상의 높은 중화 항체가를 보인 Akabane 바이러스가 추정되었다.

• 대한바이러스학회지
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• 제30권1호
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• pp.51-59
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• 2000

During the recent epidemic period ($1995{\sim}1996$), seven strains of rubella virus were isolated in Korea. To analyze phylogenetic relationship between seven Korean strains and rubella virus strains from other different geographical areas, structural genes (E1, E2 and C) of Korean strains were enzymatically amplified and automatically sequenced. The sequence similarities of the E1, E2 and C genes of the cosmopolitan types were $95.8{\sim}98.1%$, $92.6{\sim}99.2%$ and $96.4{\sim}99.3%$ based on 1,441, 122 and 139 nucleotides and $96.9{\sim}98.5%$, $90{\sim}100%$ and $97.8{\sim}100%$ based on 480, 40 and 46 amino acids compared to the sequences of strain RA27/3, respectively. In contrast, the sequence similarities of the E1, E2 and C genes of the Asian types were $91.5{\sim}92.1%$, $83.6{\sim}88.5%$ and 91.4% based on nucleotides and $96.9{\sim}97.7%$, 85.5% and 97.8% based on amino acids compared to the sequences of strain RA27/3, respectively. However, immunodominent epitopes of the E1 gene of the cosmopolitan and Asian types were well conserved, and the growth patterns in cell culture and immunofluorescent antibody titers in cross-reaction test showed no differences between two different types. In phylogenetic analysis based on nucleotide sequences of each gene regions, the cosmopolitan and Asian types formed two distinct phylogenetic lineages. These data showed two distinct genotypes of rubella viruses cocirculated in Korea, but no significant differences in the antigenicity of two different rubella virus strains were found.

• Journal of Plant Biotechnology
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• 제29권4호
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• pp.287-293
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• 2002

병독성 대장균 (K88ac)의 pilin gene을 분리하여 이를 당근에 도입한 후 이의 발현을 유도하여 자돈 설사병 예방 및 치료를 위한 당근경구백신 개발을 목적으로 하였다. 형질전환을 통해 494 세포주를 확립하였고, western분석을 통하여 0.1~4 $\mu\textrm{g}$/g의 pilin 단백질 발현을 확인하였으며 백신식물 생산에 적합한 세포주 2종 (M1-17, Y14-1)을 선발하여 포장생산 및 임상실험에 적용하였다. 쥐를 대상으로 당근 경구 투여시 병원균 항체 생성 여부와 면역 유도를 위한 최적 농도 지표를 파악하기 위하여 1주 간격으로 형질전환 당근 1g, 3g, 5g을 경구투여 한 결과 백신 당근 3g 투여시 10$\mu\textrm{g}$의 재조합 pilin 백신을 경구 투여한 것에 비해 다소 높은 항체 생성을 나타냈으며 3g의 분량이 쥐 면역 유도를 위한 적정량으로 확인하였다. 자돈에 대한 백신당근 경구 투여시 자돈 설사병 보호 효과를 정량적으로 구명하기 위하여 분석한 자돈의 일당 증체량은 형질전환 당근 투여시 평균 60g 이상 더 높은 증체량을 보였으며, 질병방제 효과를 구명하기 위하여 장독성 병원균을 인위 접종한 결과 대조구에서만 fecal score 3의 심각한 설사를 확인하였다. 본 연구를 통해 개발된 당근백신은 효율적 투여방법 등에 대한 후속 실험을 통하여 산업적 이용 가능성이 탐색될 예정이다.

• 대한수의학회지
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• 제46권4호
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• pp.347-353
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• 2006

Serum samples of 30 chickens per flock from 6 grand parent stock (GPS) farms and 70 parent stock (PS) farms were collected for seroprevalent study of pullorum disease-fowl typhoid (PD-FT) infection by serum plate agglutination test (SPA). The incidence of PD-FT infection in GPS flocks and PS flocks were 0% and 15.7%, respectively. Especially PS flocks infected with PD-FT showed age dependent patterns that 22.2% of flocks between 20 to 30 weeks of age and 38.9% of flocks between 30 to 40 weeks of age were positive. The incidence of GPS flocks and PS flocks using Salmonella (S.) gallinarum 9R (SG9R) live vaccine were 33.3% and 58.6%, respectively. The sero-positive rate of 11 flocks were 6.7-83.3% by SPA and 2.9-55.6% by enzyme linked immunosorbent assay (ELISA), and ELISA showed more lower antibody levels than SPA. Furthermore, specific antibodies produced by SG9R vaccination were detectable by SPA using SG9R antigen without cross-reaction with the PD-FT infection.

• 대한임상검사과학회지
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• 제39권2호
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• pp.104-112
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• 2007

The purpose of this study was to evaluate the general features of allergic patients in northwestern Chungcheongnamdo who visited Soonchunhyang University Cheonan Hospital. The subjects in this study were 1692 suspected allergic patients. After their allergic symptoms were checked and a MAST-CLA test was conducted, the following results were obtained: 1. The mean age of the subjects was 23.1 years old. The male patients represented 56.2% and the female patients accounted for 43.8%. 1387 (82.0%) patients of those investigated suffered from allergic disease. 2. Among the 1387 patients, 1022 (73.7%) patients showed an increased total IgE level. The positive rate of those who were in their 40s (87.0%) was the highest, but their age made no difference to their positive rate of total IgE. 3. Concerning the positive rate for allergen specific antibody by age, those who were in their teens (73.5%) topped the list and similar in all age group except in their 40s. By gender, the positive rate of the male and female were 56.3% and 43.9% respectively. Regarding the positive rates by allergic disease, those who suffered from allergic rhinitis (60.4%) were most vulnerable, followed by the patients with allergic dermatitis (47.4%), with bronchial asthma (47.2%) and with urticaria (39.4%). 4. As for seasonal positive rates, they were most susceptible in April (77.2%) and May (71.1%). We discovered a significant difference according to seasons; Spring (60.1%), Winter (45.4%) and Summer (39.2%). 5. In case of Korean inhalent panel, the most dominant allergen-specific antibodies were "Cockroach mix" (31.1%), followed by "D. pteronyssius" (23.8%) and "Dog" (14.3%). In the event of food panel, the most popular allergen-specific antibodies were "D. farinae" (25.0%), followed by "D. pteronyssinus" (19.8%) and "Hose dust" (12.0%). 6. The residential type made no difference to the positive rates of "House dust", "Cockroach mix" and "Dog" as major antigen but compared with others, positive rates for "Tick" were somewhat higher in apartment buildings.

• 한국식품과학회지
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• 제30권5호
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• pp.1029-1034
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• 1998

충치유발균인 Streptococcus mutans (혈청형 C)를 면역한 산란계의 난황에서 항체를 분리하고 그 특성을 조사하였다. 먼저 난황으로 부터 항체의 추출효율을 검토하였을 때, ${\lambda}-carrageenan$, $gammaYolk^{TM}$, $EGGstract^{TM}$의 방법으로 얻은 추출물 중 항체의 순도는 전기영동에서 각각 20, 46, 48%로 나타났으며, 난황 1 g에서 얻은 항체의 수율은 각각 11.3, 1.7, 1.8 mg이었다. 정량면역침강반응의 결과, ${\lambda}-carrageenan$방법으로 얻은 조난황 항체 중 특이항체의 비율은 12.2%이었다. 이로부터 계란 하나분인 15 g의 난황에서 얻은0.85 g의 조난황 항체에는 특이항체가 100 mg가량 함유되어 있음을 알 수 있었다. 세 종류 충치유발균주(혈청형 b, c, f)에 대한 특이항체의 반응성을 조사하였을 때, 설탕 첨가배지에서 배양한 균주는 C(+), f(+), b(+)의 순으로 48%이상의 높은 반응성을 나타내었으나 무설탕 배지에서 배양한 균주의 반응성은 각각 이보다 낮게 나타났다. 조난황 항체의 열 및 pH안정성은 양호하여, $70^{\circ}C$까지 50%이상의 활성을 유지하였고 $pH\;4{\sim}8$에서 대체로 안정하였다.

• Pediatric Infection and Vaccine
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• 제3권2호
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• pp.145-153
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• 1996

The incidence of Adenovirus(AV) pneumonia in children is estimated at 8%. Long-term complications reported with type 7 include hyperleucent lung syndrome, bronchiolitis obliterans and may require several months to recover. We reviewed 8 case of AV pnemonia confirmed by AV culture and sero-diagnosis among selected 19 AV pneumonia suspicious cases who were admitted to the Sanggye Paik Hospital of Inje University due to clinical pneumonia from May to July 1996. From 19 children, nasal aspirates or trachial aspirates were collected: viral agents were detected by virus isolation and virus antigen was detected by indirect immunofluorescent staining. Serologic diagnosis for type-specific AV antibody was done by neutralisation test. The results are as follows: 1) Age of the patients ranged from 6 months to 47 months with a mean of $24{\pm}9$ months. 6 were male and 2 were female(M:F= 3:1). 2) Monthly distribution showed 4 cases on June, 2 cases on May and July, respectively. 3) The mean duration of admission was 20.4 days and mean duration of fever was 11.3 days 4) Peripheral leukocyte counts were elevated in 4 cases(50%) and findings of shift to left were showed in 4 cases(50%) and serum aminotransferase activities were elevated in 6 case(75%). 5) Radiologic findings include linear and streaky infiltration(1/8 cases), patchy and lobar consolidation (7/8 cases), pleural effusion(2/8 cases) and hyperleucency was seen in 3/8 cases at time of admission and increased to 6/8 cases on hospital discharge. 6) The AV serotypes were type 7, type 3, and type 1. 7) Oxygen supply was done in 4 cases(50%) and all 8 patients include 2 cases of type 7 treated with artificial ventilator were survived. In conclusion, the serotypes of AV pneumonia outbreaks in early summer of 1996 were type 7, 3, 1 and, as showed severe clinical course, continued follow up surveillance is necessary to the long term pulmonary complications.

• 대한핵의학회지
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• 제28권1호
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• pp.89-97
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• 1994

Although bone scan is a highly sensitive test for detecting bone metastasis, its findings are often limited in specificity and cannot be used for assessing the bone marrow. Bone marrow scintigraphy may provide useful information but previous experience with radiolabelled colloid has been disappointing. Recently, $^{99m}Tc$ labeled anti-granulocyte monoclonal antibody (anti-NCA-95 MAb) has been introduced as a new bone marrow imaging agent. To evaluate the usefulness of $^{99m}Tc$ anti-NCA MAb bone marrow scans for detecting skeletal metastasis, bone marrow scans of 44 malignant tumor patients were evaluated and compared with bone scan findings. Bone scan showed abnormal lesions in 26(59%) cases, and 18 of these patients also had an abnormal bone marrow scan. Seven of the 8 patients who had normal bone marrow scan despite bone scan lesions were confirmed to be free from metastasis. There was one case with a marrow defect despite normal bone scan but the presence of metastasis was not determined due to loss of follow up. Bone scan demonstrated a total of 64 lesions while bone marrow scan showed 38 lesions. Fifty percent (32/64) of the bone scan lesions had matching marrow defects while the remaining 50% did not. Most of these non matched lesions were suggested to be nonspecific lesions such as rib fractures or degenerative change. Meanwhile bone marrow scan was able to detect 6 new lesions not detected by bone scan, bit metastasis in each lesion was not confirmed. Bone marrow scan was also helpful in assessing equivocal bone scan lesions to be of metastatic nature in 10 patients by demonstrating a matched marrow defect. Thus $^{99m}Tc$ anti-NCA MAb bone marrow scan can help exclude metastasis in patients with nonspecific bone scan lesions and may be able to detect metastatic lesions not seen with bone scan. It appears useful as a complementary study to bone scan in evaluating malignant tumor patients.

• 한국가금학회지
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• 제39권2호
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• pp.113-120
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• 2012

본 연구를 통하여 Vero 세포에서 강독형 NDV인 Kr005주를 55대 연속 계대하여 Vero 세포에 적응된 Kr005/V 주를 작성하였다. Kr005/V 주는 Vero 세포에서 $10^{7.8}/mL$의 높은 바이러스 증식성을 보였으며, 감염세포는 cell rounding과 같은 CPE를 형성하였다. 또한 이열성(heat labile) NDV Kr005주와 달리 NDV Kr005/V 주는 내열성(thermostable)을 보였다. 계태아에서 평균 치사 시간(MDT)를 측정한 결과, 강독형인 Kr005주(49.6시간의 MDT)와 달리 Kr005/V 주는 120시간이상의 MDT를 보여 약독형 바이러스(lentogenic virus)로 분류되었다. Kr005/V 주의 HN 단백질과 F 단백질의 염기서열을 분석한 결과, HN 단백질의 443번째 아미노산에서만 변이(T에서 S)가 관찰되었다. Kr005/V 주와 Vero 세포를 사용하여 야외 닭 혈청을 대상으로 혈청중화 항체가를 측정한 결과, Kr005주와 CEF 세포를 사용하여 측정한 혈청중화 항체가와 97%의 상관성을 나타내었다.

• 한국임상수의학회지
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• 제31권5호
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• pp.449-453
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• 2014

복강 내 종괴 촉진 및 구토, 식욕부진, 발열, 체중 감소, 수면시간 증가를 보이는 한 마리의 고양이가 서울대학교 부속 동물병원에 내원하였다. 신체검사, 혈액검사와 더불어 영상학적 검사가 진행되었다. 복부 초음파, CT 검사를 통해 소장분절과 연결된 종괴가 확인되었으며, 종괴에 대한 세포학적 검사 결과 소화기 림프종으로 잠정 진단되었으며 이에 따른 항암치료가 이뤄졌다. 항암치료가 진행되는 동안 증상은 호전되지 않았고, 결국 항암치료 중단 6일 후 폐사하였다. 부검이 이뤄졌으며 최종적으로 병변은 조직병리학적 검사를 통해 고양이 전염성 복막염으로 확진하였다.