• Title/Summary/Keyword: Antibiotics resistance inhibition

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Synergy effect of legal highs with antibiotics (Legal High Plants와 항생제의 항균활성 비교)

  • Jung, Son-Hyo;You, Seon-Hee;Park, Cho-Hi
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.6
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    • pp.1635-1645
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    • 2020
  • In this study would like to find extending or increasing the efficacy of the antibiotic substance for the strains with resistance to antibiotics or persister cells by inhibition of the resistance. This study was used different species of 'legal high' plants leaves from Leonotis leonurus, Mitragyna speciosa, and seeds from Ipomoea murucoides with antibiotics which are Amoxicillin, Chloramphenicol, Ciprofloxacin, Kanamycin, Oxacillin, and Vancomycin. Legal highs were extracted with methanol. Minimum inhibitory concentration(MIC) testing for a range of antibiotics with extracts of plant was fulfilled by broth dilution methods. In this essay, it was determined in a microdilution assay utilizing suspended in ISB up to a final concentration of 512㎍/ml in 96 wells microtitre plates, threefold and serial dilutions. After that, the microplates were kept in incubator between 35℃ and 37℃ for overnight. Leonotis leonurus, Mitragyna speciosa, and Ipomoea murucoides of Legal highs (512㎍/ml) investigated small activity to inhibit against pathogens which are susceptible Staphylococcus aureus, resistant Staphylococcus aureus, susceptible Enterococcus faecalis, resistant Escherichia coli.

Doxyclycline and Ciprofloxacin Treatment of Silk to Produce Novel Infection Resistance Biomaterials

  • Park, Hyung-Min;Martin Bide;Matthew Phaneuf;William Quist;Frank Logerfo
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.10a
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    • pp.81-82
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    • 2003
  • Two antibiotics, doxycycline (doxy) and ciprofloxacin (cipro) were applied under a variety of conditions to silk, and to silk that had previously been hydrolyzed. FTIR-ATR analyses indicated that the drastic increase in sorption of antibiotics by hydrolyzed silk was attributable to both chemical and conformational changes that occurred on hydrolysis. A high sorption of doxy by hydrolyzed silk did not necessarily give a more infection-resistant material as determined by a zone of inhibition test. Conversely, the same hydrolysis considerably increased both sorption of cipro and zone of inhibition of cipro-treated silk dyed at 65$^{\circ}C$ and 85$^{\circ}C$.

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The Screening of Antibiotics Resistance Inhibition of Herb Drugs entered in Korean Official Formulary (I) (공정서 수재생약 정유의 항생제 내성억제작용 검색(I))

  • Lee, Chung-Kyu;Kim, Dong-Myeong
    • Korean Journal of Pharmacognosy
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    • v.39 no.4
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    • pp.369-380
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    • 2008
  • The essential oils from 73 aromatic herb drugs entered on Korean official formularies were tested for antibioticresistance inhibitory effect. When the oils were combined with ampicillin (Am) or amoxicillin (Amx) they showed significant inhibitory effects on the growth of multi-drug resistant Staphylococcus aureus SA2 in considerably low concentration. The most effective combination were oils from Magnoliae Flos or Piperis longi Fructus ($25{\mu}g/mL$)-Am or Amx ($4{\mu}g/mL$).

Antibacterial Activity and Inhibition of Resistance in Methicillin-resistant Staphylococcus aureus by Maneung-hwan Ethanol Extract (만응환(萬應丸) 에탄올 추출물의 메티실린 내성 포도상구균에 대한 항균활성 및 내성억제 효과)

  • Na, Yong-su;Kim, Jong-gyu;Song, Yung-sun
    • Journal of Korean Medicine Rehabilitation
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    • v.30 no.1
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    • pp.31-45
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    • 2020
  • Objectives In this study, we investigated the antimicrobial activity of a 70% ethanol extract of Maneung-hwan (MEH), which is prescribed by practitioners of oriental medicine for use against methicillin-resistant Staphylococcus aureus (MRSA). Methods The antibacterial activity of MEH against MRSA strains was evaluated using the disc diffusion method, broth microdilution method (minimal inhibitory concentration, MIC), checkerboard dilution test, and time-kill test. The mechanism of action of MEH was investigated by bacteriolysis using detergents or ATPase inhibitors Additionally, mRNA and protein expression were investigated by quantitative reverse transcription-polymerase chain reaction and western blot assay, respectively. Results The MIC of MEH was 25~1,600 ㎍/mL against all the tested bacterial strains. We showed that MEH extract exerts strong antibacterial activity. In the checkerboard dilution test, the fractional inhibitory concentration index of MEH in combination with antibiotics indicated synergism or partial synergism against S. aureus. The time-kill study indicated that the growth of the tested bacteria was considerably inhibited after a 24-h treatment with MEH and selected antibiotics. To measure the cell membrane permeability, MEH (3.9 ㎍/mL) was combined with Triton X-100 (TX) at various concentrations N,N-dicyclohexylcarbodimide (DCCD) was also tested as an ATPase inhibitor. TX and DCCD cooperation against S. aureus exhibited synergistic action. Accordingly, the antimicrobial activity of MEH in the context of cell membrane rupture and ATPase inhibition was assessed. Additionally, the expression of genes and proteins associated with resistance was reduced after exposing MRSA to MEH. Conclusions These results suggest that MEH possesses antibacterial activity and acts as a potential natural antibiotic against MRSA.

A Study on the Antibacterial Activity of Combined Administration of Jakyakgamcho-tang and Antibiotics Against MRSA (MRSA에 대한 작약감초탕과 항생제 병용투여의 항균활성에 관한 연구)

  • Dam Hee Kang;Ok Hwa Kang;Hee-Sung Chae;Dong Yeul Kwon
    • Korean Journal of Pharmacognosy
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    • v.54 no.2
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    • pp.72-79
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    • 2023
  • MRSA is Staphylococcus aureus resistant to β-lactam antibiotics, and is a worldwide infectious disease. Even with the discovery of new antibiotics, resistance develops rapidly, so new alternatives are needed. Jakyakgamcho-tang (JGT) is a combination of Jakyak and Gamcho, and has been mainly used as an antispasmodic and analgesic in oriental medicine. This study was conducted to find out whether there is an effect on MRSA in relation to the anti-inflammatory effect of JGT and the antibacterial effect of Jakyak and Gamcho found in previous studies. In this study, in order to investigate the antibacterial activity of JGT and the combined effect of existing antibiotics, after extracting JGT with 70% EtoH, the disc diffusion method, minimum inhibitory concentration (MIC), drug combination effect (FICI), and time-kill analysis (Time-kill assay), metabolic inhibition, Western blot and qRT-PCR analysis were used to confirm the antibacterial activity mechanism of MRSA of JGT. As a result of the experiment, all of MRSA showed antibacterial activity in JGT's disc diffusion method, and the MIC was 250-1000 ㎍/mL. When existing antibiotics and JGT were combined with drugs, most had synergy or partial synergy. In addition, it was confirmed that the degree of bacterial growth was suppressed over time when simultaneous administration for 24 hours. JGT showed a synergistic effect when administered together with the ATPase-inhibitor DCCD, suggesting that it affected the inhibition of ATPase. As a result of observing the expression of PBP2a, and hla protein in the JGT-treated group and the untreated control group through wstern blot, it was confirmed that the protein expression of the JGT-treated group was significantly suppressed, and the expression levels of mecA, mecR1 and hla genes were also suppressed during JGT treatment. was observed by qRT-PCR. Combining the results of the experiment, it can be seen that JGT has antibacterial activity in MRSA, and when combined with existing antibiotics, the effect was increased compared to treatment with the drug alone. This suggests that JGT can be an alternative to treatment for antibiotic resistance of MRSA.

Effect of Hexane Extract of Acori graminei Rhizoma on the Growth of Chloramphenicol Resistant Bacteria

  • Moon, Kyung-Ho;Kwon, Joo-Yeoul;Kim, Hye-Kyung;Seo, Bong-Soo;Lee, Chung-Kyu
    • Natural Product Sciences
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    • v.9 no.3
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    • pp.183-185
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    • 2003
  • The combination of hexane extract (E4) of rhizome of Acorus gramineus with chloramphenicol (Cm) was applied to Gram negative Cm resistant microbials to find the possibility of clinical use and to clarify the relationship of the activity of chloramphenicol acetyltransferase (CAT). The combination of $1,000\;{\mu}g/ml$ of E4 and $8\;{\mu}g/ml$ of Cm entirely ceased the growth of S. aureus SA2, a gram positive resistant strain to 10 antibiotics. But in Gram negative strains which possess CAT activity, some showed considerably strong resistances to Cm and some did weakly.

Purification and Characterization of Aminoglycoside-Resistant inhibitior from methylotrophic Actinomycetes (Methanol 자화 방선균으로부터 Aminoglycoside 내성 저해물질의 정제 및 특성)

  • 김현수;신재욱
    • Microbiology and Biotechnology Letters
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    • v.27 no.3
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    • pp.215-222
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    • 1999
  • Methylotrophic actinomycetes No. 155 produced an aminoglycoside antibiotics(AG)-resistant inhibitor. We have previously reported that the inhibitor shows strong inhibition to sisomicin-resistant strain. In order to understand the functions of inhibitor and sisomicin-resistance, characterizations and purification of inhibitor were investigated. Strain No. 155 was tentatively identified as Nocardiopsis sp. based on morphological and some physiological characteristics. In the antimicrobial activity test, the addition of inhibitor to sisomicin showed a reduction effect of MIC on the test strains such as Gram(+), Gram(-) bacteria and yeasts. The combination of the inhibitor and various antibiotics revealed synergistic against E. coli K-12 and B. subtilis PCI 219. The induced intracellular proteins from sisomicin-resistant strain exhibited the sisomicin inactivation by invitro test. And the induced intracellular proteins were inactivated by addition of the inhibitor. The inhibitor compound was purified by anion exchange chromatography(Dowex-1) and HPLC using Asahipak ES-502C column. The purified inhibitor compound was detected in a single peak(above 98.5% purity) through the HPLC analysis.

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Screening and Characterization of Pro biotic Lactic Acid Bacteria Isolated from Korean Fermented Foods

  • Lim, Sung-Mee;Im, Dong-Soon
    • Journal of Microbiology and Biotechnology
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    • v.19 no.2
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    • pp.178-186
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    • 2009
  • To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

Antibacterial Effect of Sohamhyung-tang Against Methicillin-Resistant Staphylococcus aureus (소함흉탕(小陷胸湯)의 Methicillin 내성 Staphylococcus aureus에 대한 항균활성 연구)

  • Yum, Dae Yul;Baek, Dong Ki;Song, Yung Sun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.26 no.6
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    • pp.886-893
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    • 2012
  • Methicillin-Resistant Staphylococcus aureus (MRSA) is a cephalosporin and beta-lactam antibiotic-resistant strain. In most cases, MRSA is spread from infected patients and infection rates are growing increasingly. Furthermore, increased resistance to antibiotics is causing serious problems in the world. Staphylococcus aureus is responsible for both nosocomial and community-based infections that range from relatively minor skin and soft tissue infections to life-threatening systemic infections. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases. In this study, we investigated antimicrobial activity of oriental medicine prescription against MRSA. The minimum inhibitory concentration (MIC) of Sohamhyung-tang water extract against S. aureus strains ranged from 500 to 2,000 ${\mu}g/mL$, so we have it confirmed that a strong antibacterial effect. Also, the combinations of Sohamhyung-tang water extract and conventional antibiotics exhibited improved inhibition of MRSA with synergy effect. We suggest that Sohamhyung-tang water extract against MRSA have antibacterial activity, it has potential as alternatives to antibiotic agent. We suggest that the Sohamhyung-tang water extract lead the treatment of bacterial infection to solve the resistance and remaining side-effect problems that are the major weak points of traditional antibiotics.

An Experimental Study on the Durability Properties of Repair Mortar for Sewer Spread with Liquefied Antibiotic (액상 항균제를 도포한 하수시설용 단면복구재의 내구특성에 관한 실험적 연구)

  • Lee Dong-Heck;Jang Jae-bong;Na Chul-Sung;Cho Bong-Suk;Kim Jae-hwan;Kim Moo-Han
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2005.05a
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    • pp.211-214
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    • 2005
  • Recently, Deterioration of the concrete sewer concrete structures by biochemical corrosion has been issued and a development of the inhibition system of corrosion that has been demanded. The sulfuric acid may react with the hardened cement paste and originate expansive products which can induce swelling and breakless of concrete. Also, a sulphuric acid reacts with calcium hydroxide to from $CaSO_4\;\cdot\;2H_2O$. This reaction accounts for consumption of the calcium hydroxide present in hardened cement paste. In this study, To present from biochemical corrosion of the sewer repair mortar that was spread with liquefied antibiotic and then its experimental properties were experimentally investigated and to estimate the effect of absorbed condition of restorative mortar, the number of coating times and coating contents with antibiotic on the durability properties of restorative mortar spread with antibiotics. Also, testing items such as carbonation depth, choloride ion penetration depth and chemical resistance was tested to estimate the durability properties in third study. In results, the novellus bacillus inhabiting in sewer concrete structures was restrained by antibiotics developed in this study. And carbonation depth, choloride ion penetration depth and chemical resistance of restorative mortar spread with antibiotics was superior to that of plain mortar.

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