• 약학회지
• /
• 제51권3호
• /
• pp.164-168
• /
• 2007

Antibiotic resistance patterns of 21 antibiotics were studied for 50 strains of Staphylococcus aureus isolated from a hospital in Busan from July 2005 to December 2006. All strains showed antibiotic resistance to more than one antibiotic and 3 strains showed resistance to 17 different antibiotics. The strains isolated between 2005 and 2006 had lower resistance rate to 12 antibiotics (other than vancomycin and ampicilin) than the strains isolated between 1989 and 1990. In particular, no chlorarmphenicol resistant strain was found in this study which is contrasted with 34.8% resistant rate obtained in the study conducted between 1989 and 1990. In respect of vancomycin, no resistant strain was found in this study which is the same result obtained in the 1989 to 1990 study; All strains investigated in this study showed 100% resistance rate to ampicillin compared to 69.6% in the previous study.

• 한국유기농업학회지
• /
• 제9권4호
• /
• pp.135-148
• /
• 2001

Antibiotics supplementation in animal feeds results in bacterial resistance to the antibiotics and residue of the antibiotics in animal products, which can cause serious problems in human health. Therefore, the finding of new substances replacing antibiotics are needed. New substances are egg york antibody, probiotics, organic acid, mannanoligosaccharide(MOS), fructooligosaccharide(FOS), and chitosan etc. Egg york antibody is antibody to obtain from egg york of the chicken injected the specific antigen. Probiotics can prevent the problems of residue of the antibiotics and resistance to the antibiotics. Organic acids ctrl preservation of colostrum inhibit the rottenness and increase the beneficial bacteria. MOS and FOS increase the beneficial bacteria, too. Chitosan is used the immune material to active the anti-bacteria.

• 대한미생물학회지
• /
• 제22권2호
• /
• pp.155-162
• /
• 1987

To determine the prevalence of antibiotic resistance in fecal E. coli and to investigate possible associations between antibiotic resistance and other plasmid-mediated virulence properties, antibiotic disk susceptibility tests for nine antibiotics were done on 141 strains of E. coli isolated from diarrheal children and well controls. Eighty two percent of the test strains were resistant to one or more antibiotics. Antibiotics to which the test strains were most resistant in descending order were ampicillin (85%), trimethoprim/sulfamethoxazol (60%), and cephalothin (55%). Seventy nine percent of these resistant strains were resistant to two or more antibiotics. All 141 test strains were sorted into enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroadherent E. coli (EAEC) and non-pathogenic E. coli and the percentages of strains resistant to multiple antibiotics were compared. Among ETEC regardless of its source, multiple drug resistance was more frequent in strains producing heatstable enterotoxin (ST) only than in strains producing only heat-labile enterotoxin (LT) or both. In EAEC, multiple resistance was more frequently associated with strains isolated from diarrheal patients than with those from well controls. The major antibiotic resistance patterns possessed by multiple resistant enteropathogenic strains were $SXT^R$ $AM^R$, $CR^R$, and $SXT^R$ $AM^R$ $CR^R$. Of 28 ST- producing $SXT^R$ ETEC, 26(96%) were also resistant to ampicillin and 17 (61%) were resistant to cephalothin. The similar pattern was observed in EAEC and EPEC as well. This study has important implications for the treatment of E. coli diarrhea with antibiotics because it is possible that dissemination of virulence could occur under the force of selective antibiotic pressure. In addition, this study suggests that the in vivo efficacy of SXT in treating diarrheal illness be reevaluated.

• 한국미생물·생명공학회지
• /
• 제51권4호
• /
• pp.432-440
• /
• 2023

Irrational and injudicious use of antibiotics, easy availability of them as over-the-counter drugs in economically developing countries, and unavailability of regulatory policies governing antimicrobial use in agriculture, animals, and humans, has led to the development of multi-drug resistance (MDR) bacteria. The use of medicinal plants can be considered as an alternative, with a consequent impact on microbial resistance. We tested extracts of Piper longum fruits as new natural products as agents for reversing the resistance to antibiotics. Six crude extracts of P. longum fruits were utilized against a clinical isolate of multidrug-resistant Staphylococcus aureus.The antibiotic susceptibility testing disc method was used in the antibiotic resistance reversal analysis. Apart from cefoxitin and erythromycin, all other antibiotics used (lincosamides [clindamycin], quinolones [levofloxacin and ciprofloxacin], and aminoglycosides [amikacin and gentamicin]) were enhanced by P. longum extracts. The extracts that showed the greatest synergy with the antibiotics were EAPL (ethyl acetate [extract of] P. longum), n-BPL (n-butanol [extract of] P. longum), and MPL (methanolic [extract of] P. longum The results of this study suggest that P. longum extracts have the ability to increase the effectiveness of different classes of antibiotics and reverse their resistance. However, future studies are needed to elucidate the molecular mechanisms behind the synergy between antibiotic and phytocompound(s) and identify the active biomolecules of P. longum responsible for the synergy in S. aureus.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2005년도 추계 학술논문 발표대회
• /
• pp.97-100
• /
• 2005

Recently sewage facilities mainly consisted of concrete structures are being deteriorated seriously by biodeterioration originated from sulfur-oxidizing bacteria. In this study, to prevent biochemical corrosion of the sewer concrete, antibiotics which prevent the growth of sulfur-oxidizing bacteria were developed and antimicrobial performance of it was investigated. After that, to consider applicability of antibiotics to concrete, durability such as resistance to carbonation, salt damage and chemical attack of concrete covered with inorganic and complex antibiotics were investigated. As a result of this study, it was proved that the antimicrobial performance of antibiotics was available. Also resistance to carbonation, salt damage and chemical attack of concrete covered with inorganic antibiotics was little improved but, in case of complex antibiotics, was remarkably improved. Moisture content of concrete, as a application condition of antibiotics in whole case, have little effect on performance but covering times of antibiotics have effect on performance only in case of complex antibiotics.

• Natural Product Sciences
• /
• 제6권1호
• /
• pp.36-39
• /
• 2000

Acorenone, a diterpene isolated from Acorus gramineus, showed strong resistance inhibitory activity against multi-drug resistant microorganisms such as Staphylococcus aureus SA2, which has resistance to 10 usual antibiotics including chloramphenicol (Cm). At the level of $5\;{\mu}g/ml$ when combined with $50\;{\mu}g/ml$ of Cm. Bacterial resistance to Cm is due to the presence in resistant bacteria of an enzyme, chloramphenicol acetyltransferase (CAT), which catalyses the acetyl-CoA dependent acetylation of the antibiotic at C-3 hydroxyl group. To elucidate the mechanism of resistant inhibitory effect, the acorenone which had the strongest resistant inhibitory activity, was investigated on the CAT assay. As the result, the combination of Cm and acorenone showed the strongest inhibitory activity on CAT as noncompetitive and dose dependent manner.

• 약학회지
• /
• 제35권1호
• /
• pp.22-29
• /
• 1991

pMB4 is a 2.4-kilobase plasmid of Staphylococcus aureus TR-1 that confers inducible resistance to the macrolide-lincosamide-streptogramin B(MLS) antibiotics. By subcloning studies, it was found that the MLS resistance determinant was located at 1.0Kb fragment between Sau3AI and TaqI sites. DNA sequence of the MLS resistant determinant, named ermC-4 was determined, and found to be highly homologous with that of ermC. Because the leader peptide sequence of ermC-4 was identical with that of ermC, the expression of the resistance gene is thought to be controlled by posttranscriptional attenuation in S. aureus TR-1.

• 대한수의학회지
• /
• 제49권3호
• /
• pp.195-200
• /
• 2009

Avian pathogenic Escherichia coli (APEC) is an important bacterial pathogen of chickens and causes colibacillosis such as airsacculitis, perihepatitis, omphalitis, peritonitis, salpingitis, and pericarditis. As the transfer of antibiotic resistance from animal to humans can be possible, surveillance on antibiotic resistance of APEC is very important. A total 34 APEC isolates from diseased chickens during the period from 2007 to 2009 were obtained. The susceptibility of the isolates to 13 antibiotics was determined by disc diffusion assay. Resistance to erythromycin was found in 97.1% of APEC isolated, followed by resistance to tetracycline (85.3%), doxycycline (82.3%), ampicillin (73.5%), sulfisoxazole (67.6%), enrofloxacin (67.6%), ciprofloxacin (64.7%), norfloxacin (61.7%) trimethoprim/sulfamethoxazole (52.9%), gentamycin (26.5%), amoxicillin (8.8%), colistin (5.9%), and amikacin (2.9%). The blaTEM genes were detected in 25 (100%) of the 25 ampicillin-resistant APEC isolates. Among the 29 tetracycline-resistant APEC isolates, tetA and tetB genes were detected in 18 (62.1%) and 9 (31%) isolates, respectively. Twenty six (76.5%) isolates were multiresistant to at least 6 antibiotics and seven (20.1%) isolates were multiresistant to at least 10 antibiotics. This results indicated that multiple antibiotic-resistant APEC is widespread in chicken flocks in Korea.

• 대한미생물학회지
• /
• 제22권4호
• /
• pp.413-425
• /
• 1987

In the present experiments, isolated Vibrio species from marine and clinical specimens from July, 1985 to October, 1986, had the results as follows: 1. The 55 strains of Vibrio were isolated and identified; Vibrio parahaemolyticus was 35 strains, Vibrio vulnificus was 10 strains, Vibrio alginolyticus was 10 strains. 2. In the K-serotyping of Vibrio parahaemolyticus, fourteen serotypes identified but three were not strains typable by the availble K-antisera. 3. In the Kanagawa phenomenon experiment of Vibrio parahaemolyticus, it proved positive reaction, 14 of 15 strains(93%) isolated from the patient and 13 of 20 strains(65%) isolated from the nature. 4. In twelve antibiotic resistance experiments, Vibrio parahaemolyticus and Vibrio alginolyticus showed 100% resistance on ampicilline, but Vibrio vulnificus showed 100% sensitivity. But all of them proved 100% sensitivity on chloramphenicol, tetracycline, nalidixic acid. 5. In the antibiotic resistance patterns, Vibrio parahaemolyticus proved that 15 strains(43%) resisted on 4 antibiotics and 5 strains(14%) resisted on 7 antibiotisc and. Vibrio vulnificus proved that 1 strain(10%) resisted on 2 antibiotics and 6 strains(60%) without resistance, Vibrio alginolyticus proved that 7 strains(70%) resisted on 3 antibiotics and 2 strains(20%) resisted on 8 antibiotics.

• 한국환경보건학회지
• /
• 제38권2호
• /
• pp.142-150
• /
• 2012

Objectives: To estimate the multi-antibiotic resistant bacterial contaminant load discharged from livestock farms, we randomly selected livestock farms specializing in cattle, swine, and fowl and collected bacterial strains from domesticated animal feces and compost samples. Problems with resistance to antibiotics are becoming worldwide issues, and as the consumption of antibiotics appears to be excessive in Korea as well, the emergence of antibiotic resistant bacteria shows the possibility to cause potentially serious social problems. Methods: To monitor multi-antibiotic resistant bacterial constituents, aerobic bacteria and Escherichia coli were isolated from domesticated animal feces and compost. Antibiotic resistance testing was performed by the disc diffusion method using 13 different antibiotics. Results: Examining the degree of sensitivity to antibiotics of the aerobic bacteria originating from domesticated animal feces, fowl feces showed the highest distribution rate (35.5%), followed by swine feces compost (23.1%), swine feces (18.2%), cattle feces (14.9%), and cattle feces compost (8.2%). Antibiotic resistance tests of aerobic bacteria and E. coli originating from domestic animals feces resulted in 83.6% and 73.5% of each strain showing resistance to more than one antibiotic, respectively. Conclusions: These results suggest that increasing multi-antibiotic resistant bacteria in the environment has a close relation to the reckless use of antibiotics in livestock.