• 대한의생명과학회지
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• 제20권2호
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• pp.77-84
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• 2014

Periodic analysis of local epidemiologic data of prevalent pathogens of blood culture can provide clinicians with relevant information to guide empirical antibiotic therapy. In this study, we analyzed a pattern of change of causative microorganisms and antimicrobial resistance at a tertiary medical center in Chungcheong province from 2003 to 2012, retrospectively. Of 70,258 blood specimens cultured, 6,063 (8.6%) were positive. Among the positive isolates, 95.9% were aerobic or facultative anaerobic bacteria, 0.1% were anaerobes, and 3.9% were fungi. Coagulase-negative Staphylococci (CoNS) (32.9%), Escherichia coli (16.7%), Staphylococcus aureus (9.1%), Klebsiella pneumoniae (6.4%), and ${\alpha}$-hemolytic Streptococcus (5.9%) were commonly isolated bacteria, and Candida albicans (1.4%) was the most commonly isolated fungi. Enterococcus faecium progressively increased but Streptococcus pneumoniae, Acinetobacter baumannii and Proteus species gradually decreased over a period of 10 years. The multidrug-resistant microorganisms such as methicillin-resistant S. aureus (MRSA), vancomycin-resistant enterococci (VRE), cefotaxime-resistant E. coli, imipenem-resistant Pseudomonas aeruginosa (IRPA) and imipenem-resistant A. baumannii (IRAB), were significantly increased. Therefore, there is a need for a more strict control of antibiotics and a more updated guideline for the treatment of bloodstream infection.

• 한국축산식품학회지
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• 제38권4호
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• pp.759-767
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• 2018

The concern about the possibility of food can be a vehicle for the transmission of Clostridium difficile to humans has been raised recently due to the similarities among the strains isolated from patients, foods and food animals. In this study, therefore, the prevalence of C. difficile was investigated in beef and chicken meat products collected from 57 different butcher shops, markets and fast food restaurants in Sakarya province of Turkey. Two out of 101 samples (1.98%) was positive for C. difficile indicating a very low prevalence. The pathogen was isolated from an uncooked meatball sample and a cooked meat $d{\ddot{o}}ner$ sample, whereas not detected in chicken meat samples. The meatball isolate was resistant to vancomycin and tetracycline, while the cooked meat $d{\ddot{o}}ner$ isolate was resistant to vancomycin and metronidazole. Both isolates were sensitive to moxifloxacin and clindamycin. Toxins A and B were not detected. This study reveals the presence of C. difficile in further processed beef products in Turkey.

• 한국미생물·생명공학회지
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• 제34권1호
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• pp.74-77
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• 2006

A lactic acid bacterial strain resistant to several antibiotics such as oxytetracycline, tylosin, neomycin and sulfathiazole, which have been often used as a therapeutic agent in livestock, was isolated from the porcine gastrointestinal tract. The isolate YB-55 was identified as belonging to the genus Pediococcus with the highest similarity to P. pentosaceus on the basis of its 16S rRNA sequence and biochemical properties. The isolated strain showed viability of over 85% at pH 3.0 and was resistant to bile salt. The strain produced lactic acid of 12.3 g/L by jar fermentation and maintained its viability in the presence of antibiotics at dosage related therapeutic effect, suggesting P. pentosaceus YB-55 may of ffr potential as a probiotics for livestock.

• Tuberculosis and Respiratory Diseases
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• 제69권1호
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• pp.39-42
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• 2010

Mycobacterium massiliense is newly identified rapid-growing nontuberculous mycobacterium, but there are no reports of this mycobacterium species being the cause of human illness. We describe one case of Mycobacterium massiliense infection presenting as antibiotic-resistant acute pneumonia that resulted in surgical treatment.

• 미생물학회지
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• 제40권1호
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• pp.17-22
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• 2004

항생제 내성균들의 등장으로 새로운 항생제의 개발이 요구되고 있으나 항생제의 개발은 많은 시간과 인력과 개발비를 필요로 한다. 이 문제를 해결하기 위한 방법의 하나로 전통적으로 감염질환의 치료에 사용되어온 식물인 황금과 황백 추출물의 methicillin-resistant Staphylococcus aureus(MRSA)에 대한 항균활성 보유 여부를 조사하였다. 황금의 메탄올 추출물은 MRSA와 Candida에 대하여 높은 항균 활성을 나타내었으며 황백 추출물보다 활성이 높아 앞으로 새로운 항균제로의 개발 및 사용 가능성이 있을 것으로 보여진다.

• Journal of Dairy Science and Biotechnology
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• 제37권1호
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• pp.40-48
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• 2019

Bifidobacteria are a prototype probiotic, which normally inhabit the intestinal tract of humans. In the present study, four species of Bifidobacterium isolated from the feces of infants were characterized. The tolerance for acid or bile salt, autoaggregation, and antibiotic resistance of the bacteria were examined. The four species were resistant to low pH, bile salts, and up to 3% bile acid. Autoaggregation rates were as high as 90%. The bacteria were consistently resistant to gentamicin, kanamycin, streptomycin, ciprofloxacin, and nalidixic acid. Due to their tolerance to environmental factors like acid and bile salts, B. longum MG723, B. breve MG729, B. bifidum MG731, and B. animalis subsp. lactis MG741 are potentially valuable as probiotics and may be useful for industrial application.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.70.2-71
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• 2003

Quinolone resistance in Streptococcus pneumoniae is related to mutations in the DNA gyrase and topoisomerase IV genes. DW-286a displayed potent activity against S. pneumoniae C9211 (MIC, 0.015 ${\mu}$g/ml) compared with gemifloxacin (MIC, 0.06 ${\mu}$g/ml). This study was performed to analyze the ability of DW-286a to cause resistance development in S. pneumoniae and to establish whether DNA gyrase or topoisomerase IV is primary target. DW-286a resistant mutants of S. pneumoniae C9211 were generated by stepwise selection at increasing drug concentration. (omitted)

• 대한임상검사과학회지
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• 제36권2호
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• pp.76-88
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• 2004

본 연구에서는 임상검체에서의 Enterococcus sp.의 균종별 분리현황을 조사하고, 분리균의 항생제 감수성 양상을 알아보는 한편, aminoglycoside계 항생제에 내성을 나타내는 균주를 대상으로 Rep-PCR을 실시하여 유전적인 다양성을 알아보고자 하였다. 각종 임상검체로부터 E. faecalis 49주, E. faecium 34주, E. avium 9주, E. gallinarum 4주, E. casseliflavus 3주, E. hirae 1주 등 총 100주를 분리하였다. 대부분 외래를 통하기보다는 입원환자의 검체로부터 분리됨을 관찰하면서 원내감염의 원인균 중의 하나임을 알 수 있었다. Urine과 pus, sputum에서 대부분의 균들이 분리되었고 E. faecalis와 E. faecium이 주 종을 이루었다. 항생제 감수성양상으로는 disk법과 ViteK GPS-430의 MIC결과와 유사하게 관찰 되었으며, 대부분의 Enterococcus sp.가 cephalosporin계 항생제에는 80% 정도의 내성을 보였다. E. faecalis의 경우 항생물질에 대해 고른 감수성양상을 볼 수 있었고, E. faecium의 경우 다른 Enterococcus sp.와는 차별적으로 항생물질에 대해 높은 내성 양상을 볼 수 있었다. 또한 vancomycin에 대한 내성을 보이는 Enterococcus sp.는 전체 10%정도를 차지하였고 aminoglycoside계 항생제인 amikacin과 gentamicin에 대해서 높은 고도내성을 나타냈었고, streptomycin의 경우에는 50% 정도의 내성률을 나타내었다. Aminoglycoside계 항생제에 내성을 보이는 Enterococcus sp.를 대상으로 Rep-PCR을 실시하여 유전자 분획유형을 분석하였으나 유전적 다양성에 있어 크게 차이를 보이지 않아 DNA양상의 기원이 크게 다르지 않다는 것으로 생각되었다.

• Journal of Ecology and Environment
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• 제48권1호
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• pp.17-23
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• 2024

Background: Honey bees play a crucial role in pollination and ecological balance. Apis mellifera L. colonies, especially those located in specific geographic regions, such as the palm garden in Eastern Thailand, are susceptible to potential threats from microbial contaminants. Understanding and detecting microbial organisms in these beehives is essential for the preservation of bee health, honey production, and the broader ecosystem. However, the problem of microbial infection and antibiotic-resistant bacteria is more severe and continuously increasing, resulting in a health, economic, and social crisis. The purpose of this study is to determine the prevalence of microorganisms in A. mellifera beehives in palm gardens in Rayong province, Eastern Thailand. Results: Ten swabs in transport media were swabbed and obtained from different parts of each beehive (1 swab per beehive), for a total of 10 hives. Traditional microbial culture-based methods, biochemical tests, and antimicrobial susceptibility (disc-diffusion) tests were used to detect microbial organisms and antibiotic resistance in bacteria. The swab tests from nine beehives resulted in the detection of Gram-positive bacteria (63.64%), Gram-negative bacteria (27.27%), and fungi/yeast (9.09%). These microorganisms are classified as a group of coagulase-negative Staphylococcus spp. and made up 40.91% of the bacteria discovered. Other bacteria found were Coryneform bacteria (13.64%), Pantoea spp. (13.64%), Bacillus spp. (9.09%), yeast (9.09%), glucose non-fermentative Gram-negative bacilli (9.09%), and Pseudomonas spp. (4.55%). However, due to the traditional culture-based and 0biochemical tests usually used to identify the microbial organisms in clinical specimens and the limitation of identifying some environmental microbial species, the results of the antimicrobial susceptibility test cannot reveal if the organism is resistant or susceptible to the drug. Nevertheless, drug-sensitive inhibition zones were formed with each antibiotic agent. Conclusions: Overall, the study supports prevention, healthcare, and public health systems. The contamination of microorganisms in the beehives may affect the quality of honey and other bee products or even the health of the beekeeper. To avoid this kind of contamination, it is therefore necessary to wear personal protective equipment while harvesting honey and other bee products.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 2005년도 제57차 추계 학술대회 연제집
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• pp.487-487
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• 2005