• 한국막학회:학술대회논문집
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• 한국막학회 2004년도 Proceedings of the second conference of aseanian membrane society
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• pp.121-123
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• 2004

Sulfonamide moiety, RNSO$_2$, has been studied as biologically active compounds such as antibacterials.$^1$ Bissulfonylimide group,-SO$_2$NHSO$_2$-, has been introduced to the side chain of Nafion$^{(R)}$ to be the base moiety for an acid doping.$^2$However, to the best of our knowledge, there has been no report on SULFAMIDE ACID(Scheme 1), one of the sulfonic acid derivatives.(omitted)d)

• Bulletin of the Korean Chemical Society
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• 제11권4호
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• pp.313-317
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• 1990

Geometries for several representative quinolone carboxylate type antibacterials have been calculated by computer graphics/molecular mechanics energy minimization procedures using both MM2 and AMBER force fields. The calculated geometries were found to be in reasonable agreements with the corresponding X-ray crystal structures. It has been pointed out that notwithstanding the weaknesses associated with calculating the resonance and hydrogen bonding contributions, the employed methods are capable of generating credible ring geometries and torsional angle dispositions of N(1)-ethyl and 3-carboxylate substituents of the quinolones.

• Toxicological Research
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• 제13권1_2호
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• pp.111-116
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• 1997

In the present study, we investigated the neutralization activity of various antimicrobials against lipopolysaccharide (LPS) as well as interaction between two antimicrobials (enrofioxacin and coilstin) using checkerboard method. The neutralization activity of antimicrobials used in the test was assayed by means of LAL chromogenic test after reaction of LPS with colistin, enorfioxacin, ampicillin, polymyxin B, oxytetracycline, streptomycin, and erythromycin. As the results, the neutralization activity of coltstin and polymixin B had a more stronger than that of tested other antimicrobials. In bacterial culture broth, the best neutralization activity of the antibiotics was also shown to coltstin and polymixin B. Meanwhile, It was shown to have synergism between enorfloxacin and coltstin on the basts of FIC (fractional inhibition concentration). The FIC of enorfioxacin-colistin combinations was 0.50-1.03 to Staphylococcus aureus R-209, 1.03-1.06 to Salmonella typhimurium, 0.75-1.25 to Bordetella Bronchtseptica and 1.02-1.25 to E. coli K88ab. On the basts of the above results, the present study may be of clinical usefulness in the choice of an antibiotic therapy for severe sepsts in animals.

• 한국식품위생안전성학회지
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• 제8권1호
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• pp.1-8
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• 1993

육류중에 잔류하는 항생물질 및 항균제를 검출하기 위해 본 실험에서는 3종의 균주 Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, BAcillus cereus var. mycoides ATCC 11778을 사용하여 실험하였다. 시료의 clean-up은 항생·항균제의 물리화학적 성질을 고려하여 우선 McIlvaine buffer를 가하여 homogenize하고 hexane으로 defatting 시킨 후, chloroform으로 추출한 액과 Sep-Pak C18과 Bakerbond SPE carboxylic acid column에 흡착시킨 후 추출한 액을 각각 시험용액 A, B, C,로 하였다. 각 test solution을 paper disk를 사용하여 함균 배지에 올려놓고 overnight culture 후 inhibition pattern을 통해 여러 종류의 항생·항균제를 계통적으로 검출하였다. 본 실험에서 macrolide계와 tetracycline계 등은 0.1ppm 이하의 detection limit을 보였으며, penicillinrP는 0.001ppm이하의 높은 detection limit을 나타내므로서 시료중에 잔류하는 극미량까지도 검출할 수 있었다. 본 방법은 식육중의 잔류 항생·항균제를 동시에 간단하게 계통적으로 분류하는데 있어 좋은 방법이라고 생각되며 항생·항균제의 체계적인 1차 screening 수단으로서 유용한 방법이라 사료된다.

• 생약학회지
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• 제19권4호
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• pp.262-269
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• 1988

The Forsythiae fructus is described to be used as an antiinflammatory drug, diuretics, antidotes and antibacterials in oriental literatures. In order to investigate the efficacy of Forsythiae viridissima (Oleaceae), the methanol extract and its fraction have been evaluated for the acute toxicity, antiinflammatory, analgesic and spasmolytic action in animals. The methanol extract of Forsythiae fructus was found to have significant antiinflammatory activity in the acute and subacute antiinflammatory model in rats, but have no analgesic action. Furthermore, through fractionation procedure, it was found that the active compounds were easily soluble in chloroform and butanol. It is also noted that the extracts had spasmolytic activities in the rat fundus and uterus and had low acute toxicity in mice.

• 대한수의학회지
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• 제39권2호
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• pp.365-369
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• 1999

A study on quinolone antibacterial, norfloxacin, was performed to apply for the control of bacterial diseases in eel (Anguilla japonica). Norfloxacin was proved excellent in antibacterial activity and sensitivity against fish bacterial pathogens when compared with the existing antibacterials and antibiotics. And any side effect was not observed during the period of indicated use. An outline of minimal inhibitory concentration was $0.03{\sim}0.1{\mu}g/ml$, $TLm_{48h}$ value was 3,500mg/l. The residual time of the day in fish body was less than 17 days and any pathological changes were not observed. The study has revealed that norfloxacin can be applied to treat some fish bacterial disease by the dosage of 100g/day/ton of fish body weight for about 3 days perorally. Further, norfloxacin may be used for the control of bacterial pathogens in eel.

• 한국어병학회지
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• 제9권2호
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• pp.195-201
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• 1996

군산근교의 양만장에서 애드와드병에 이환된 뱀장어로부터 총 96균주의 E.tarda균을 분리한 후 이들 분리균의 약제감수성 검사를 실시하고 아울러 내성균의 내성인자 전달 시험을 실시하였다. 공시한 모든 균주는 SM, AK, NF 및 GM등에는 100% 감수성을 보였으나, 대부분의 균주가 SD(86균주), AM(84균주), PM(80균주), NA(67균주), OT(44균주) 및 OA(37균주) 등의 내성을 보였다. 내성유형은 20유형으로서 거의 모든 균주가 2제 이상의 다제내성을 보였으며, 그중 SD-AM-PM-NA-OA(16균주), SD-AM-PM-NA(14균주), SD-AM-PM-NA-OT-OA(12균주), SD-AM-PM-OT(10균주) 및 SD-AM-PM-NA-OT(8균주) 유형등이 고빈도로 출현하였다. 내성균주의 내성인자 전달 빈도는 94균주중에서 78균주가 단계에서 6제까지의 다제내성인자의 전달을 보였다. 그중 AM 및 AM-PM-NA유형(8균주), PM,SD 및 AM-SD유형(6균주), AM-PM, PM-SD 및 AM-SD-OT유형(4균주)등이 고빈도 출현을 보였다. 이러한 결과는 양만장에서 다양한 항균제를 사용하였기 때문에 다제내성현상이 확산되어진 것으로 사료된다.

• 한국응용과학기술학회지
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• 제17권1호
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• pp.37-42
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• 2000

Drug delivery system(DDS) have been actively studied for the past twenty years. Dual action agents are unique chemical entities comprised of two different types of antibacterial compounds covalently linked together in a single molecule in such a way that both components are able to exert their bactericidal properties. In spite of the advent of the antibacterial agent the sulfa agents are the most widely used antibacterial agent today. In this study, new antibacterials derivative was synthesized using glutaraldehyde such as crosslinking agent for the purpose of dual-action as DDS study. Antibacterial activity of these new synthetic derivative between their structures and activities were examined by disc diffusion method. As a result, new synthetic derivative exhibited the broad antibacterial activities against Gram(+) and Gram(-) bacilli. Especially, the antibacterial effect of new synthetic derivative against Gram negative(Esherichia. coli) was much stronger than that against Gram positive.

• Fisheries and Aquatic Sciences
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• 제19권10호
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• pp.45.1-45.5
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• 2016

As a novel strategy to remove ${\beta}$-lactam antibiotic residues from fish tissues, utilization of ${\beta}$-lactamase, enzyme that normally degrades ${\beta}$-lactam structure-containing drugs, was explored. The enzyme (TEM-52) selectively degraded ${\beta}$-lactam antibiotics but was completely inactive against tetracycline-, quinolone-, macrolide-, or aminoglycoside-structured antibacterials. After simultaneous administration of the enzyme with cefazolin (a ${\beta}$-lactam antibiotic) to the carp, significantly lowered tissue cefazolin levels were observed. It was confirmed that the enzyme successfully reached the general circulation after intraperitoneal administration, as the carp serum obtained after enzyme injection could also degrade cefazolin ex vivo. These results suggest that antibiotics-degrading enzymes can be good candidates for antibiotic residue depuration.

• 한국어병학회지
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• 제21권1호
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• pp.29-34
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• 2008

손바닥선인장(Opuntia ficus-indica var. saboten Makino) 줄기 가수분해 분획물(MBT-01108)의 어병세균에 대한 in vitro 항균활성을 조사하였다. 실험에 사용한 어병세균은 Edwardsiella tarda, Listonella anguillarum, Vibrio sp., Lactococcus garvieae, L. raffinose, Streptococcus parauberis, S. iniae 등 총 18종이었다. 또한, oxytetracycline을 포함한 시판 항균제 disc를 MBT-01108의 항균활성에 대한 양성 대조군으로 하여 항균활성을 비교 조사하였다. 그 결과, MBT-01108은 실험에 사용한 어병세균들에 대하여 우수한 항균활성을 나타내었다.