• 동의생리병리학회지
• /
• 제17권2호
• /
• pp.574-579
• /
• 2003

Methicillin-resistant Staphylococcus aureus (MRSA) has been emerging worldwide as one of the most important hospital and community pathogens. Therefore, new agents are needed to treat the MRSA. In the present study, we investigated antimicrobial activity of ethyl acetate, methanol, and water extracts of Curcuma longa L. (C. longa) aganist clinical isolates of MRSA. The ethyl acetate extract of C. long a demonstrated a higher antibacterial activity than the methanol extract or water extract. Since the ethyl acetate extract was more active than other extracts, we examined whether ethyl acetate extract may restore the antibacterial activity of β-lactams and alter the adhesion and invasion of MRSA to human mucosal fibroblasts (HMFs). In the checkerboard test, ethyl acetate extract of C. longa markedly lowered the MICs of ampicillin and oxacillin against MRSA. In the bacterial adhesion and invasion assay, MRSA intracellular invasion were notably decreased in the presence of 0.125 - 2 mg/ml of C. longa extract compared to the control group. These results suggest that ethyl acetate extract of C. longa may have antibacterial activity and the potential to restore the effectiveness of β-lactams against MRSA, and inhibit the MRSA adhesion and invasion to HMFs.

• 한국유기농업학회지
• /
• 제21권2호
• /
• pp.219-232
• /
• 2013

로즈마리의 주요성분으로는 carnosic acid, carnosol, rosmarinic acid, ursolic acid 등의 폴리페놀(polyphenolic)로 구성되어 있다. 식물기원의 페놀혼합물은 항산화 작용과 항균작용을 포함한 유용한 기능과 영양적인 효과에 주목을 끌고 있다. 본 연구는 로즈마리 추출물의 하나인 카르노스산을 화이트소시지 원료에 첨가한 후 실온 저장기간 동안 pH, TBARS, VBN, 전자공여능 및 총균수의 변화를 조사하였다. 카르노스산의 첨가는 무첨가구인 대조구에 비해 낮은 pH, TBARS값, 그리고 VBN값을 보여 주었으며, 높은 항산화 작용과 항균작용을 나타내었다. 이들의 활성은 카르노스산의 농도가 높을수록 증가하였다. 또한 카르노스산의 병원성균에 대한 항균활성을 측정한 결과, 두 병원성균(E. coli KCCM 11234와 S. enteritidis KCCM 12021)에 대하여 강한 항균활성을 보여주었다. 따라서 화이트소시지에 있어서 카르노스산은 천연보존제로서 충분히 이용 가능한 결과를 나타내었다.

• 공업화학
• /
• 제18권1호
• /
• pp.17-23
• /
• 2007

제지용 무기안료에 항균기능과 광촉매 탈취기능을 부여하기 위해 hybridization system을 이용하여 Ag 용액과 $TiO_2$로 표면 개질 처리하여 기능성 무기안료를 제조하고, 이를 이용하여 항균 및 탈취 기능을 갖는 특수지를 제조한 후 내항균 특성을 평가하였다. 내항균 평가로는 균의 생성여부를 눈으로 직접 확인 할 수 있는 halo test법, 균들의 생육 저하효과를 확인하는 방법인 inhibition growth test와 정균감소법을 사용하였다. 또한 제조된 항균기능성 특수지를 벽지용도로 적용하고자 하였으며, 내항균 평가 시 항균벽지 주위로 선명한 클리어 존이 형성되어 균의 성장 억제를 육안으로 확인할 수 있었다. 항균 및 탈취기능을 지니는 특수지의 휘발성 유기화합물 분해능 평가를 위한 benzene의 광촉매 분해 효율실험에서 반응시간 80min 동안 45~50%의 제거효율을 보였으며, 반응시간 30 min 정도에서 분해효율이 90% 이상 도달함을 알 수 있었다.

• 대한치과교정학회지
• /
• 제48권3호
• /
• pp.163-171
• /
• 2018

Objective: The aim of this study was to evaluate the mechanical and biological properties of orthodontic bonding agents containing silver- or zinc-doped bioactive glass (BAG) and determine the antibacterial and remineralization effects of these agents. Methods: BAG was synthesized using the alkali-mediated solgel method. Orthodontic bonding agents containing BAG were prepared by mixing BAG with flowable resin. $Transbond^{TM}$ XT (TXT) and $Charmfil^{TM}$ Flow (CF) were used as controls. Ion release, cytotoxicity, antibacterial properties, the shear bond strength, and the adhesive remnant index were evaluated. To assess the remineralization properties of BAG, micro-computed tomography was performed after pH cycling. Results: The BAG-containing bonding agents showed no noticeable cytotoxicity and suppressed bacterial growth. When these bonding agents were used, demineralization after pH cycling began approximately 200 to $300{\mu}m$ away from the bracket. On the other hand, when CF and TXT were used, all surfaces that were not covered by the adhesive were demineralized after pH cycling. Conclusions: Our findings suggest that orthodontic bonding agents containing silver- or zinc-doped BAG have stronger antibacterial and remineralization effects compared with conventional orthodontic adhesives; thus, they are suitable for use in orthodontic practice.

• 한국식품조리과학회지
• /
• 제33권3호
• /
• pp.256-263
• /
• 2017

Purpose: Essential oils are secondary metabolites of herbs and have antibacterial activities against foodborne pathogens. However, their applications for food protection are limited due to the hydrophobic and volatile natures of essential oils. Methods: In this study, essential oil nanoemulsions of rosemary and lavender were formulated with non-ionic surfactant Tween 80 and water using ultrasonic emulsification, and their antibacterial effects were determined. Results: The antibacterial activities of nanoemulsions were evaluated against 12 strains of 10 bacterial species, and significant antibacterial effects were observed against four Gram-positive and four Gram-negative bacteria but not against Streptococcus mutans and Shigella sonnei. In the disc diffusion test, the diameter of the inhibition zone proportionally increased with the concentration of nanoemulsions. Using cell turbidity measurement, minimum bactericidal concentration (MBC) of the nanoemulsions, which is the lowest concentration reducing viability of the initial bacterial inoculum by ${\geq}99.9%$, was significantly higher than the minimum inhibitory concentration (MIC) of the nanoemulsions. The largest bactericidal effects of lavender and rosemary essential oil nanoemulsions were observed against S. enterica and S. aureus, respectively. Conclusion: Nanoemulsion technique could improve antibacterial activity of essential oil nanoemulsions by increasing the solubility and stability of essential oils. Our findings shed light on the potential use of essential oil nanoemulsions as an alternative to chemical sanitizers in food protection.

• Journal of Applied Biological Chemistry
• /
• 제63권3호
• /
• pp.249-257
• /
• 2020

본 연구는 코스모스꽃 추출물이 박테리아에 대해서 항균 활성이 있는지를 알아보고 이를 통해 항균 효과를 내는 물질에 대해 규명하고자 하였다. 흰색, 분홍색, 자주색의 코스모스꽃이 4종의 박테리아인 S. aureus, S. epidermidis, P. aeruginosa, E. coli에 대해 항균 효과가 있는지 접종 후 24 h 동안 관찰하였다. 3종의 꽃 추출물 중에 EtOAc층 분획물에서 4개의 박테리아에 대해 가장 우수한 항균 활성이 관찰되었고 S. aureus에 대해서는 CHCl₃층 분획물도 효과를 보였다. 추가적으로 흰색 코스모스꽃 silica gel Fr. 1과 분홍색 코스모스꽃 silica gel Fr. 2, 자주색 코스모스꽃 silica gel Fr. 1은 0.1 mg/mL에서 최소저해농도가 나타났다. 이 분획물들과 코스모스 내에 존재할 것으로 유추되는 apigenin 간의NMR과 HPLC 비교, 분석한 결과를 통해서 유효 성분내에는 apigenin구조 요소가 함유되어 있는 물질이 있을 것으로 판단되었다.

• 대한화장품학회지
• /
• 제44권1호
• /
• pp.59-66
• /
• 2018

Propionibacterium acnes (P. acnes)는 여드름의 주요 병원성균으로 염증성 질환에 관련된 미생물 중에 하나이다. 본 연구에서는 여드름 주요 유발 균주인 P. acnes에 대하여 70% Ethanol로 추출한 제주 자생식물 추출물의 항균활성을 disc diffusion test 실험을 이용하여 측정하였다. 실험 결과 61개의 제주 자생 식물 추출물 중에서 45개 추출물의 항균 활성이 검출되었으며, 16개의 추출물은 항균 활성이 검출되지 않았다. 그중에서 여우 구슬(Phyllanthus urinaria L.) - 줄기 잎 추출물이 $18.96{\pm}0.69mm$로 가장 높은 항균 활성을 나타내었고, 참당귀(Angelica gigas Nakai) - 뿌리, 돌외(Gynostemma pentaphyllum (Thunb.) Makino), 참당귀(Angelica gigas Nakai) - 잎 줄기, 산박하(Isodon inflexus (Thunb.) Kudo), 차나무(Camellia sinensis L.) - 꽃, 그리고 로즈마리(Rosmarinus officinalis L.) - 줄기 추출물 순으로 높은 항균 활성을 나타내었다. 이러한 결과는 제주 자생 식물 추출물이 여드름 치료 예방의 항균제 및 기능성 화장품 천연 원료로써의 이용 가능성을 제안한다.

• KSBB Journal
• /
• 제22권2호
• /
• pp.78-83
• /
• 2007

제주도 자생식물의 80% 메탄올 추출물 10종의 항균 효과 및 항산화 효과를 검색하였다. 실험 대상 식물은 생체 채집하여 음건한 후 세절하여 80% 메탄올로 추출하여 추출된 시료를 10% 농도로 희석한 후 어류 질병미생물에 대한 항균실험을 하였고 80% 메탄올 추출물에 대한 항산화 실험은 각각의 시료를 0.1%, 0.5%, 0.8%, 1.0%, 2.0%로 희석하여 각 식물의 항산화 효과를 측정하였다. 항균 실험은 어류 질병미생물로 분류된 그람 음성균 12종과 그람 양성균 1종에 대하여 디스크 확산법으로 측정하였는데 그 결과 두메꿀풀 꽃과 잎 추출물에서 가장 강한 항균 효과를 나타내었고 다음으로 풀고사리 잎과 돌잔고사리 잎, 들깨 잎, 들깨 열매 순으로 나타났으며 큰천남성 뿌리나 왕모시풀 잎, 열매에서는 눈에 띄는 항균 효과를 나타내지는 않았다. 그리고 항산화 효과를 측정하기 위해 DPPH radical 소거능과 SOD 유사활성, Hyoxyl radical 소거 활성을 실험하였다. 각 식물의 80% 메탄올 추출물을 농도별로 희석하여 측정 한 결과 농도가 높아질수록 항산화 효과가 증가하는 것을 확인 할 수가 있었으며 2.0% 농도에서 두메꿀풀 잎과 열매 그리고 들깨 잎과 열매, 돌잔고사리 잎은 합성 항산화제인 BHT, BHA와 활성을 비교하여 보았을 때 BHT, BHA보다 높거나 유사한 항산화 효과를 나타내었다.

• Bulletin of the Korean Chemical Society
• /
• 제24권10호
• /
• pp.1478-1484
• /
• 2003

CRAMP was identified from a cDNA clone derived from a mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptide. Tertiary structure of CRAMP in TFE/$H_2O$ (1 : 1, v/v) solution has been determined by NMR spectroscopy previously and consists of two amphipathic $\alpha-helices$ from Leu4 to Lys10 and from Gly16 to Leu33. These two helices are connected by a flexible region from Gly11 to Gly16. Analysis of series of fragments composed of various portion of CRAMP revealed that an 18-residue fragment with the sequence from Gly16 to Leu33 (CRAMP-18) was found to retain antibacterial activity without cytotoxicity. The effects of two Phe residues at positions 14 and 15 of CRAMP-18 on structure, antibacterial activity, and interaction with lipid membranes were investigated by $Phe^{14,15}$ ${\rightarrow}$ Ala substitution (CRAMP-18-A) in the present study. Substitution of Phe with Ala in CRAMP-18 caused a significant reduction on antibacterial and membrane-disrupting activities. Tertiary structures of CRAMP-18 in 50% TFE/$H_2O$ (1 : 1, v : v) solution shows amphipathic ${\alpha}$-helix, from $Glu^2{\;}to{\;}Leu^{18}$, while CRAMP-18-A has relatively short amphipathic ${\alpha}$-helix from $Leu^4{\;}to{\;}Ala^{15}$. These results suggest that the hydrophobic property of $Phe^{14}{\;}and{\;}Phe^15$ in CRAMP-18 is essential for its antibacterial activity, ${\alpha}$-helical structure, and interactions with phospholipid membranes.

• The Journal of Advanced Prosthodontics
• /
• 제7권2호
• /
• pp.166-171
• /
• 2015

PURPOSE. The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system. MATERIALS AND METHODS. Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction. RESULTS. The number of S. mutans colonies on the TiN, ZrN and $(Ti_{1-x}Zr_x)N$ coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05). CONCLUSION. The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and $(Ti_{1-x}Zr_x)N$ coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.