• 치위생과학회지
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• 제8권4호
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• pp.367-373
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• 2008

천연물 황금으로부터 여러 유기용매 추출물을 얻어 치아우식증 원인균인 S. mutans 에 대한 항균활성, 배지 내 환경변화, 그리고 부착억제에 대해 알아보았다. 1. 구강 질병인 치아우식증의 대표적 원인균 S. mutans에 대한 황금 ethyl acetate 추출물 (IPK-3)의 최소억제농도는 125 mg/ml 이었다. 2. 황금 IPK-3 추출물 50 mg/ml의 농도로 S. mutans의 배지 내 투여하였을 때 균의 생장 량과 최대 생장시간은 대조군보다 지연되었다. 3. 황금 IPK-3 추출물 50 mg/ml의 농도로 S. mutans의 배지 내 투여하였을 때, pH 변화는 대조군은 18시간에서 pH 5.63으로 급격한 변화를 보였으며 황금 IPK-3 추출물이 50 mg/ml의 배지에서는 6.50이상을 유지하여 pH가 변화가 없었다. 4. 황금 IPK-3 추출물을 첨가한 배지 내 탄수화물, 단백질 및 균체 외 다당류의 변화는 대조군에 비해 매우 낮게 생산되었다. 5. 균체를 형광염료(DAPI)로 염색하여 S-HA 부착억제을 확인한 결과, 대조군은 시간이 경과함에 따라 S.mutans의 hydroxyapatite(HA)에 부착 정도가 증가되었으나, 황금 IPK-3 추출물이 첨가된 시험군에서는 S-HA에 균의 부착이 매우 적었다. 이같이 황금의 IPK-3 (ethyl acetate 추출물)이 치아우식증의 원인균인 S. mutans 에 대한 항균활성효과 뿐만아니라 hydroxyapatite에 부착 억제 효과도 있었다.

• 생약학회지
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• 제9권2호
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• pp.77-82
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• 1978

Free anino acid contents in alcohol extract of eight plants of Caryophyllaceae and their microbial activities were investigated. 1) Amino acid contents in both of the Pseudostellaria palibiniana and Stellaria media was the highest among them and the contents was less in the order of Cerastium caspitosum and Stellaria aquatica. 2) Of all free amino acids contained in eight plants, valine was the richest, and then glutamic acid, leucine in that order. On the other hand, no methionine was observed and cystine, lysine and histidine were found in small amounts. 3) Of eight plants exhibited good antibacterial action against Sarcina lutea, B. subtilis and Sal. typhi. 4) S.aquatica and Pseudostellaria palibiniana showed antibiotic actions against all bacteria except for fungus, Candida albicans. 5) C. caspitosum and C. brachypetalum showed inhibition zone against B. subtilis and Sal. typhi only. 6) Antibacterial activity against gram(+) bacteria was more potent than gram(-).

• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1605-1612
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• 2012

The present study concerned the identification and characterization of a novel bacterial strain isolated from entomopathogenic nematodes collected from different regions in Korea. The bacterial isolate M1021 was Gramnegative, bioluminescent, and produced red colonies on MacConkey agar medium. A rod-shaped structure was confirmed by the electron micrograph. Fatty acid composition was analyzed by using the Sherlock MIDI system. The identification was further supported by 16S rDNA sequence analysis, which revealed 96-99% sequence homology with strains of Photorhabdus temperata. The location of the isolated strain of P. temperata in the phylogenetic tree was confirmed and it was named P. temperata M1021. P. temperata M1021 exhibited catalase, protease, and lipase activities when grown on appropriate media supplemented with respective substrates. The culture of P. temperata M1021 exhibited insecticidal activity against the larvae of Galleria mellonella and the activity was the highest after 3-4 days of cultivation with agitating at $28^{\circ}C$ under 220 rpm. Antibacterial activity was also observed against Salmonella Typhimurium KCTC 1926 and Micrococcus luteus KACC 10488.

• 미생물학회지
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• 제36권3호
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• pp.236-241
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• 2000

효모 Saccharomyces cerevisiae를 숙주 세포로 이용하여 활성형의 항균펩티드를 생산하기 위한 model system으로서 쉬파리 유래 defensin의 합성 유전자를 GAP promoter, mating factor $\alpha$1 (MF$\alpha$1)의 preprosequence 및 GAL7 transcription terminator의 조절하에 있는 재조합 plasmid pGMD18을 제작한 후 Saccharomyces cerevisiae에 형질전환하여 growth inhibtion zone assay를 통해 항균활성을 보유한 재조합 효모를 선별하였다. 재조합 효모의 성장 속도와 defensin의 분비능을 증가시키기 위하여 최적의 배지 조성과 배양조건을 결정하였다. 재조합 효모의 defensin 생산을 위한 최적 배양조건을 조사한 결과 0.4% yeast extract, 유기질소원 2% corn steep liquor, 탄소원 2.5% glucose, 0.05% $C_2CO_3$를 포함한 배지에서 초기 pH3 그리고 배양온도 $28^{\circ}C$의 경우가 세포성장과 defensin 의 항균활성이 가장 우수하였다. 이 조건으로 배양을 수행한 결과 YPD 배지에서 배양한 조건보다 약 2배의 세포 성장과 약 4배의 defenzin 생산을 확인하였다.

• Archives of Pharmacal Research
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• 제19권2호
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• pp.143-147
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• 1996

LB20304 is a novel fluoroquinolone that exhibits a potent broad spectrum antibacterial activity against both gram-positve and gram-negative bacteria. The MICs (Minimal Inhibitory Concentration) of LB20304 were determined against both gram-positve and gram-negative bacteria under various conditions including several media, pHs, and inoculum concentrations. The in vitro activity of LB20304 was not significantly affected by the changes in testing conditions such as components of media and inoculum concentrations, but it was slightly reduced by acid condition. The MICs and MBCs (Minimal Bactericidal Concentration) of LB20304 against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were hardly affected by the presence of 50 % human serum, mouse serum, guinea pig serum or horse serum, and the MBCs were equal to or at most four-times higher than the MiCs. The activities of LB20304 were decreased by the presence of high concentraion of $Mg^{++}$ or human urine (pH, 5.5) in the test media. The frequencies of mutants resistant to LB20304 were similar to or lower than those found in ciprofloxacin and sparfloxacin.

• 폴리머
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• 제36권1호
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• pp.71-75
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• 2012

본 연구는 폴리(비닐 알코올)(PVA) 하이드로젤 안에 $AgNO_3$ 용액을 이용하여 은 나노입자(AgNPs)를 제조하였다. PVA 입자를 증류수에 용해시킨 후, 50 kGy 감마선을 조사하여 PVA 하이드로젤을 제조하였다. 감마선을 이용하여 제조된 PVA 하이드로젤을 0.01, 0.05 M 질산은 수용액에 1시간 동안 침지후, 팽윤된 하이드로젤을 꺼내 감마선을 재조사하여 PVA 하이드로젤 내부에 AgNPs를 제조하였다. AgNPs가 함유된 PVA 하이드로젤의 UV 흡수 특성 및 FE-SEM 측정 결과, 감마선 조사량이 증가할수록 AgNPs의 생성이 증가하는 것을 확인할 수 있었으며, 같은 조사량에서 $AgNO_3$ 용액의 농도가 클수록 AgNPs 생성이 증가하였다. 액체배지 및 고체배지를 이용하여 그람 음성 세균인 E.coli와 S.aureus에 대한 PVA 하이드로젤에 생성된 AgNPs의 항균 실험 결과 매우 우수한 항균 효과를 나타냈다.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.418-422
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• 2001

Growth inhibition of Compylobacter jejuni ATCC 33291 was observed in the presence of various preservatives at various temperatures. The addition of ethanol (0.5% to 5%), hydrogen peroxide (0.05%), acetic acid (1%), propionic acid, benzoic acid, and sorbic acid showed strong antibacterial activities against C. jejuni at pH 5.5 or 6.5. The addition of 1% acetic acid and lactic acid were most effective at $42{\circ}C, followed by $25{\circ}C$ and $4{\circ}C$. This indicated that the inhibitory effect was temperature dependent. In the chicken model system, the practical death rate of C. jejuni in the FBP-media with 1% acetic temperatures ($4{\circ}$C$, $25{\circ}$C, and $42{\circ}$). Therefore, precaution has to be taken in the use of organic acids as a disinfectant in the chicken slaughterhouse.

• 약학회지
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• 제43권4호
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• pp.437-441
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• 1999

Enoxacin[1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-\piperazinyl)-1,8-naphthyridine-3-carboxylic acid, ENX] is a new quinolone antibacterial agent. The method is based on the highly colored charge-transfer complex formation of this drug as a $\pi$-electron donor with 7,7,8,8-tetracyanoquinodimethane(TCNQ) or chloranil(CL) as $\pi$-acceptors. The colored products were measured spectrophotometrically at 842 nm and 552 nm for TCNQ and CL, respectively. The different experimental conditions are optimized. The linearities for TCNQ and CL were $1.6{\;}\mu\textrm{g}/mL~32{\;}\mu\textrm{g}/mL$ and $6.4{\;}\mu\textrm{g}/mL~160{\;}\mu\textrm{g}/mL$, respectively and colors were produced in non-aqueous media. This report describes a simple and ra\pid method for the analysis of enoxacin.

• 대한의생명과학회지
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• 제24권2호
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• pp.116-124
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• 2018

Lysozyme is known as a key substance of the innate immunity and have antibacterial effect in the mucosal tissues, especially middle ear. Aquaporin (AQP) functions as water movement in the tissue and has been expected to be participated in the inflammatory responses. In the present study, we investigated to reveal association of lysozymes and AQPs in otitis media. The gene expression of lysozyme genes, homo sapiens lysozyme (hLYZ), homo sapiens lysozyme M (hLYZ M), and homo sapiens lysozyme G like-2 (hLYGH), and AQP genes (AQP 0 - AQP 12) were measured from postauricular skin, mastoid mucosa, inflamed mastoid mucosa, and middle ear mucosa. The hLYZ, hLYZ M and hLYGH gene were expressed in mastoid mucosa, inflamed mastoid mucosa, middle ear mucosa. Of AQP genes, all AQP gene except AQP 3 gene were expressed in the tissue of middle ear. Among them, AQP 4, AQP 8, AQP 9, AQP 10, AQP 11 and AQP 12 were highly expressed in the inflamed mastoid mucosa and normal mastoid mucosa (P<0.001). Interestingly, expression levels of AQP 4, AQP 9, and AQP 12 gene were significantly higher in the inflamed mastoid mucosa compared to normal middle ear mucosa (P<0.05). These results suggest that lysozyme and AQPs could be associated with inflammatory response in the middle ear.

• 농업과학연구
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• 제15권1호
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• pp.76-81
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• 1988

세균용(細菌用) 항생제(抗生劑)인 chlorampenicol과 streptomycin sulfate의 참깨역병균(疫病菌) (Phytophthora nicotianae var. parositica)에 대(對)한 항균효과(抗菌效果)를 구명하기 위하여 실시(實施)한 실험결과(實驗結果)는 다음과 같다. 1. PDA배지(培地)에서 chlorampenicol은 10ppm, streptomycin sulfate는 25ppm의 낮은 농도(濃度)에서 참깨역병균(疫病菌)의 균사생장(菌絲生長)을 매우 강력(强力)하게 억제(抑制)하였고 유주자낭(遊走子囊) 형성(形成)을 완전(完全)히 조지(阻止)하였으며 각각 5ppm, 10ppm에서도 균사생장(菌絲生長)과 유주자낭(遊走子囊) 형성억제(形成柳制) 효과(效果)가 있었고 다른 방제약제(防除藥劑)와의 혼용시(混用時) 더 강력(强力)한 억제효과(抑制效果)가 나타났다. 2. 각(各) 처리(處理) 모두 균체내(菌體內) 단백질함량(蛋白質含量)이 감소(減少)하였으며 균사(菌絲)의 이상신장(異常伸長)이 관찰(觀察)되었다. 3. 유묘(幼苗)에 살포시(撒布時) 두 약제(藥齊) 모두 참깨에 약해(藥害)는 없었다.