• Title/Summary/Keyword: Antibacterial media

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Comparison of Antibacterial Ability of Air Filter Media Treated with a Natural Antibacterial Agent by Three Treatment Methods (항균성 천연물질의 도포방법에 따른 에어필터 여재의 항균 특성 비교)

  • Park, Sun Young;Jung, Jae Hee;Hwang, Gi Byung;Bae, Gwi-Nam;Kim, Yong Pyo;Nho, Chu Won
    • Journal of Korean Society for Atmospheric Environment
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    • v.29 no.2
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    • pp.125-134
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    • 2013
  • Various antimicrobial technologies have been developed to inactivate bioaerosols in indoor environments. In this study, air filter media were treated with a natural antibacterial agent of Sophora flavescens in order to inactivate the bacteria collected on the air filter. Three treatment methods were applied to manufacture the test antibacterial air filter media: electrospray, nebulization and dip-coating methods. In case of electrospray and nebulization processes, the size distribution of aerosolized natural antibacterial agent was measured using a scanning mobility particle sizer. Staphylococcus epidermidis bacteria were aerosolized to test inactivation of the filter media treated with a natural antibacterial agent. The pressure drop and the antibacterial efficiency of the filter media increased with increasing the mass loading of natural antibacterial agent in each treatment method. The antibacterial efficiency per loaded antibacterial agent mass by the electrospray method was the highest and the filter treated by the dip-coating method was the lowest among three treatment methods.

Enumeration and Recovery of Bacterial Isolates from Ruminants Fed with Different Dietary Regimes and Their Antibacterial Activity

  • Pattnaik, P.;Grover, Sunita;Batish, V.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.6
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    • pp.811-815
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    • 2001
  • The study evaluated different synthetic and semisynthetic media for maximal recovery of rumen bacteria and expression of their antibacterial activity. Rumen Glucose Cellobiose Agar (RGCA) medium was found to be the best for recovery of rumen bacteria. However, L-10 medium was the best for expression of antibacterial activity of ruminal isolates followed by Easy, M-10, RGCA and M-98-5 medium. The present study recommends the use of L-10 medium as the medium of choice for screening of antibacterial activity of ruminal isolates. Comparative evaluation of bacterial counts on different dietary regimes indicated significant difference between different growth media on a specific diet and between diets on specific growth media within a species. However, there is no overall significant difference between total bacterial counts obtained from rumen liquor of cattle and buffalo with respect to either the feeding regime or growth media. Feeding straw based diet to the animal is the best for high recovery of rumen bacteria.

A Study of high Efficiency anti-Bacteria Filters for Passenger Car (객차용 고효율 항균 기능성 필터에 관한 연구)

  • 정우성;박덕신;정진도;류해열
    • Proceedings of the KSR Conference
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    • 2002.10b
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    • pp.975-980
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    • 2002
  • We actually installed antibacterial air filter on the air intake route to air conditioner in passenger car and evaluated filters performance on antibacterial effect. And also, antibacterial effect was observed at whole are of filter media by zone of inhibition test. In the evaluation of antibacterial test, it was shown that the air filter has notably sufficient antibacterial efficiency against ordinary filter.

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A Study on Antibacterial Assesment of High Efficiency Antibacterial Air Filter (고효율 항균 필터의 항균력 평가에 관한 연구)

  • 권혁구;정진도;류해열;정우성;박덕신
    • Proceedings of the KSR Conference
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    • 2002.05a
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    • pp.549-554
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    • 2002
  • Recently, interest in hygiene has been arouse in the health care field. Consequently, Filters with antibacterial agent applied to improve air quality by sterilizing bacteria, fungi, etc. We actually installed antibacterial air filter containing 2.5wt%Ag zeolite on the air intake route to air-conditioner in passenger cu, and evaluated filter's performance on antibacterial effect. By the microbe liquid spattering method, we found that the antibacterial air filter has notably sufficient antibacterial efficiency against standard strains and wild type strains. Antibacterial effect was observed at whole area of filter media by zone of inhibition test. The evaluation of microbe quantity was conducted through mixing dilution plate culture method. In comparison with ordinary filter, the amount of germs attached on antibacterial air filters was larger. The amount of germs attached on ordinary filters was very small since ordinary filters contained less dust. In comparison in antibacterial air filter with thickness, the amount of germs attached on 9mm filter was smaller than that of on 6mm filter. i.e. thicker filter, superior efficiency.

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Antibacterial Activity of Macromycetes Mycelia and Culture Liquid

  • Krupodorova, Tetiana A.;Barshteyn, Victor Yu.;Zabeida, Elena F.;Pokas, Elena V.
    • Microbiology and Biotechnology Letters
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    • v.44 no.3
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    • pp.246-253
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    • 2016
  • The antibacterial activities of thirty mushroom species belonging to Basidiomycetes and Ascomycetes, cultivated on two liquid media, were evaluated against gram-positive (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria by the disk diffusion method. All of the mushrooms, except Auriporia aurea, Fomes fomentarius, and Lyophyllum shimeji, showed different antibacterial activity levels—from 9.5 mm in diameter of the inhibition zone to full inhibition of growth of the test bacteria. The antibacterial activities of Crinipellis schevczenkovi, Hohenbuehelia myxotricha, Oxyporus obducens, and Spongipellis litschaueri were observed for the first time. The antibacterial potential of culture liquids of the investigated species was higher than that of their mycelia activity. Dependence of the intensity of antibacterial activity on the culture medium was shown. The antibacterial efficiency of the most active species (Lentinus edodes, Piptoporus betulinus, and Phellinus igniarius) was verified and compared with those of some commercial antibiotics and natural essential oils of Salvia and Eucalyptus. The culture liquid of Piptoporus betulinus, obtained after cultivation on glucose-peptone-yeast culture medium, is a potential substance for further creation of antibacterial products.

Studies on the Biological Function and Antibacterial Effect of Lactoperoxidase System in Raw Milk 2. Antibacterial Effect of Lactoperoxidase System Against Listeria monocytogenes (우유내의 LP system의 생리기능 및 항균성에 관한 연구 2. Listeria monocytogenes에 대한 항균효과)

  • 정충일;남은숙;김대원;이원창;정동관
    • Journal of Food Hygiene and Safety
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    • v.13 no.2
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    • pp.83-86
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    • 1998
  • This study was carried out to measure the antibacterial effect of lactoperoxidase system against L. monocytogenes. When the initial inoculum levels ($10^{2},\;10^{4},\;10^{7}\;CFU/ml$), concentration of LP (10 ppm, 20 ppm, 30 ppm), culture media (TSB-YE, UHT milk) and storage temperatures ($5^{\circ}C,\;10^{\circ}C,\;15^{\circ}C$) were set up differently for the experiment and the antibacterial effect was compared, the highest antibacterial effect of LP system was shown at $10^{2}\;cfu/ml$ of initial inoculum level, 10 ppm of LP concentration and $5^{\circ}C$ of incubaction temperature. The antibacterial effect of LPS in UHT milk was similar to that in Tryptic soy broth.

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Silver Coating on the Porous Pellets from Porphyry Rock and Application to an Antibacterial Media (반암(맥반석)으로 제조한 다공성 펠렛의 Ag 담지 및 항균 메디아로서의 적용)

  • Han, Yo-Sep;Kim, Hyun-Jung;Shin, Young-Seop;Park, Jai-Koo;Ko, Jae-Churl
    • Journal of the Korean Ceramic Society
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    • v.46 no.1
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    • pp.16-23
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    • 2009
  • The porous pellets were prepared from porphyry by slurry foaming method. The effect of sintering temperatures on pore structure of porous porphyry pellets with different extension ratio ($E_R$) was investigated by specific surface area, water absorption and porosity, which changed with sintering temperatures. When the sintering temperatures increased from $975^{\circ}C$ to $1075^{\circ}C$, specific surface area and water absorption of the all samples decreased. In case of the sample with an equal sintering temperature, $E_R=3.0$ pellets had little influence on pore structure compared to the $E_R=2.0$ pellets. As a results, it was shown by SEM that facilitated formation of micro pores at $E_R=2.0$ pellets shrunk increasingly after sintering process. At $E_R=3.0$ and sintering temperature at $1025^{\circ}C$, optimum conditions of the porous porphyry porous pellets was found. Also, Escherichia coli removal efficiency of the silver-containing porphoyry porous pellets was measured for the feasibility as a antibacterial media. The antibacterial activity of prepared silver-containing sample was maintained above 90% for 40 days.

Inactivation and Filtration of Bioaerosols Using Carbon Fiber Ionizer Assisted Activated Carbon Fiber Filter (탄소섬유 이오나이저를 적용한 활성탄소섬유 필터의 바이오에어로졸 항균 및 집진 성능평가)

  • Kim, Doo Young;Park, Jae Hong;Hwang, Jungho
    • Particle and aerosol research
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    • v.6 no.4
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    • pp.185-192
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    • 2010
  • This paper reports that the installation of a carbon fiber ionizer in front of an activated carbon fiber(ACF) filter enhanced the antibacterial efficiency. In addition, the effect of the ionizer on the filtration of bioaerosols is reported. Negative air ions from the ionizer were used as antibacterial agent. The test bacteria(Escherichia coli) were aerosolized using an atomizer and were deposited on the ACF filter media for 10 minutes. E. coli deposited on the filter were exposed to negative air ions for 0, 1, 5 and 10 minutes. Then they were separated from the ACF filter by shaking incubation with nutrient broth for 4 hours. The separated E. coli were spread on nutrient agar plates and incubated at $37^{\circ}C$ for 1~3 days. The antibacterial efficiency of E. coli was measured using a colony counting method. The antibacterial efficiencies of E. coli exposed to negative air ions for 1, 5 and 10 minutes were 14%, 48% and 71%, respectively. The filtration efficiency was evaluated by measuring the number concentration of bioaerosols at the upstream and downstream of the filter media. The increase of filtration efficiency by air ions was 14%, that is similar to the 17% filtration efficiency by none air ions. The ozone concentration was below the detection limit (under 0.01ppm) when the carbon fiber ionizers were on.

Diversity and Antibacterial Activity of Lactic Acid Bacteria Isolated from Kimchi (김치로부터 분리한 젖산균의 다양성과 항균성)

  • 김사열;안대경;한태원;신현영;진익렬
    • Microbiology and Biotechnology Letters
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    • v.31 no.2
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    • pp.191-196
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    • 2003
  • This study was carried out to investigate the isolation, identification, and antibacterial activity of lactic acid bacteria related to kimchi fermentation. Diluted kimchi soup was plated on the MRS agar media with CaCO$_3$ and incubated at $25^{\circ}C$ for 2 days. A total of 27 strains of lactic acid bacteria from various indigenous, spontaneously fermented vegetables (kimchi) were isolated. Combined methods of Bergey's manual of systematic bacteriology, BPB media analysis and 16S rDNA sequence analysis were applied for identification, however, their results did not coincide in several cases. Isolated lactic acid bacteria could be classified by the 16S rDNA sequence analysis as Leuconostoc mesenteriodes, Leu. carnosum, Lactobacillus curvatus, Lac. pentosus, Weisselia kimchi, W. cibaria, and Pediococcus pentosaceus. Leu. carnosum has not been reported in kimchi lactic acid bacteria. In addition, antibacterial activities of the isolates were tested with Bacillus subtilis, Escherichia coli, Salmonella enteritidis, S. paratyphica, S. typhi, Staphylococcus aureus, Shigella boydii, and S. sonnei. Some of isolates showed significant antibacterial activities to those pathogens.

Studies on the Biological Function and Antibacterial Effect of Lactoperoxidase System in Milk (우유내의 LP system의 생리기능 및 항균성에 관한여구 1. Escherichia coli O157:H7에 대한 항균효과)

  • 정충일;남은숙;김대원;전형일
    • Journal of Food Hygiene and Safety
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    • v.13 no.1
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    • pp.1-5
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    • 1998
  • This study was carried out to measure the antibacterial effect of lactoperoxidase/ thiocyanate/hydrogen peroxide system (LP system) against E. coli 0157:H7. When the initial inoculum levels (($10^{2},\;10^{4},\;10^{7}cfu/ml$), concentration of LP (10 ppm, 20 ppm, 30 ppm), culture media (TSB-YE, UHT milk, raw milk) and storage temperatures ($5^{\circ}C,\;10^{\circ}C,$15$^{\circ}C$) were set up differently for the experiment and the antibacterial effect was compared, the highest antibacterial effect of LP system was shown at $10^{2}cfu/ml$ of initial inoculum level, 10 ppm of LP concentration and $5^{\circ}C$ of incubation temperature.

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