• 생명과학회지
• /
• 제21권10호
• /
• pp.1355-1363
• /
• 2011

많은 임상 실험을 통해 마늘(Allium sativum)이 심혈관 질환에 유효한 효과가 알려져 있다. 흑마늘(AGE)은 항산화, 항염증, 항암 효과 등 다양한 생물학적 효과가 보고되었다. 그러나, 흑마늘의 심혈관계 질환과의 상관관계에 대한 연구는 아직 미흡한 실정이다. 흑마늘과 그 유래 성분이 혈소판에 미치는 영향을 조사하였다. 백서의 혈액을 얻어, 흑마늘과 그 유래 성분의 thrombin에 의해 유도되는 혈소판 응집에 대한 억제 효과를 조사한 결과, 흑마늘과 diallyl sulphides는 농도의존적으로 혈소판 응집을 억제함을 관찰하였다. 또한 in vivo 실험에서도 마늘 오일과 S-Allylmercapto-cystein (SAMC)는 thrombin과 ADP에 의해 유도되는 혈소판 응집을 유의성 있게 억제하는 것을 관찰하였다. 흑마늘 유래 SAMC와 diallyl sulphides가 혈소판 응집 억제 효과를 나타내는 것을 확인할 수 있었다. 이러한 결과는 흑마늘이 심혈관계 질환의 예방을 위한 건강보조식품으로의 새로운 가능성을 제시한다.

• Archives of Pharmacal Research
• /
• 제29권10호
• /
• pp.898-903
• /
• 2006

The antiplatelet and antithrombotic activities of Korean Red Ginseng (KRG) were examined on rat carotid artery thrombosis in vivo, and platelet aggregation in vitro and ex vivo. Administration of KRG to rats not only prevented carotid artery thrombosis in vivo in a dose-dependent manner, but also significantly inhibited ADP- and collagen-induced platelet aggregation ex vivo, while failed to prolong coagulation times such as activated partial thromboplastin time (APTT) and prothrombin time (PT), indicating the antithrombotic effect of KRG might be due to its anti platelet aggregation rather than anticoagulation effect. In line with the above observations, KRG inhibited U46619-, arachidonic acid-, collagen- and thrombin-induced rabbit platelet aggregation in vitro in a concentration-dependent manner, with $IC_{50}$ values of $620{\pm}12$, $823{\pm}22$, $722{\pm}21$ and $650{\pm}14\;{\mu}g/mL$, respectively. Accordingly, KRG also inhibited various agonists-induced platelet serotonin secretions as it suppressed platelet aggregation. These results suggest that KRG has a potent antithrombotic effect in vivo, which may be due to antiplatelet rather than anticoagulation activity, and KRG intake may be beneficial to the individuals with high risks of thrombotic and cardiovascular diseases.

• 한국미생물·생명공학회지
• /
• 제42권4호
• /
• pp.417-421
• /
• 2014

수태(Sphagnum palustre)는 물이끼과(Sphagnaceae)의 연한 녹색 선태식물로 분재, 기저귀, 수술용 붕대, 토양 첨가제 등으로 이용된다. 지구상의 거의 모든 곳에서 생육하며 매우 저렴하게 생산되지만, 이의 성분 및 생리활성에 대한 연구는 미미한 실정이다. 본 연구에서는 수태의 열수 추출물과 이의 순차적 유기용매 분획물을 조제한 후 혈액응고 저해활성과 혈소판 응집저해를 평가한 결과, 열수 추출물의 물 잔류물에서 강력한 혈액응고 저해활성과 혈소판 응집 촉진 활성을 동시에 확인하였다. 본 연구결과는 수태가 항혈전제로 개발 가능함을 제시하며, 이는 수태의 항혈전 활성에 대한 최초의 보고이다.

• 동의생리병리학회지
• /
• 제18권2호
• /
• pp.534-537
• /
• 2004

We produced twelve monoclonal antibodies(mAbs) against thyroglobulin and characterized the bindig profiles. Among them, three mAbs(TN-1, TN-2 and TN-3) were further characterized their binding specificities. TN-2 had a potent lupus anticoagulant activity and potentiated the anticoagulant effect of venom phospholipase A₂. he anticoagulant mechanism of TN-2 was elongation of the partial thromboplastin time and binding to phosphatidylserine which may have a pivot role in blood coagulation. And TN-2 was cross-reacted with ss-DNA and ds-DNA and had a characteristic of autoantibody. These results suggest that TN-2 may provide a useful tool for studying the correlation between autoimmune thyroiditis and its therapeutic effect.

• Biomolecules & Therapeutics
• /
• 제5권3호
• /
• pp.239-245
• /
• 1997

Trimetoquinol (TMQ) and its analogs are known to have thromboxane $A_2$ antagonistic action. We also reported that GS389, chemically similar to TMQ, has competitive antagonistic action in rat aorta and human platelets. In the present study, we investigated the pharmacological characteristics of GS283 and GS 386, analogs of GS389, using vascular smooth muscle, human platelets and rat brain homogenates. In isolated pig coronary artery (PCA), both of GS283 and GS386 relaxed U46619-contracted rings in concentration dependent manner. Pretreatment with several concentrations of GS283 and GS386 shifted the dose-response curves to the right, and reduced of maximum contration dose-dependently. Furthermore, GS283 and GS386 strongly inhibited $Ca^{2+}$ -induced contraction in the PCA. In human platelets, U46619- and A23187-induced platelet aggregation was inhibited by GS283 and GS386, concentration-dependently. Anti-platelet aggregation was related to the compound\`s ability to inhibit ATP release at each stimulation. In rat brain homogenates, receptor-binding assay resulted that both GS283 and GS386 have a relative affinity to $\alpha$-adrenergic receptor. Taken together. we concluded that the mechamism of action of GS283 and GS86 is not related with in TXA$_2$ receptor but concerned with calcium antagonistic action and a-blocking action.n.

• 대한한방내과학회지
• /
• 제18권1호
• /
• pp.69-85
• /
• 1997

This study was designed to elucidate the anti-inflammatory, cardiovascular, anti-thrombotic and analgesic effects of Shintongchugeotang. The anti-inflammatory effect was measured by the method of carragenin induced edema, protein leakage test using CMC-pouch, and the analgesic effect was measured by the acetic acid method and hot plate method, and the effect of Shintongchugeotang on the cardiovascular system was observed by the change of flow rate of Ringer solution in the vascular system in the ear of rabbit, and the contraction and dilatation of rat tail artery. Death rate, platelet aggregation, plasma coagulation activity was observed for the measurement of the anti-coagurative effect of Shintongchugeotang. The result was as follows : 1. After the administration of Shintongchugeotang extract, Carragenin induced edema and CMC-pouch protein leakage were significantly decreased. 2. The slight analgesic effect of Shintongchugeotang extract was confirmed by the observation of writhing syndrome, paw licking time, and escape time. 3. The drug increased the auricular blood flow in rabbit. 4. The drug relaxed the artery contraction by pretreated norepinephrine in rat. 5. The drug inhibited the death rate of mouse which was led to thromboembolism by serotonin and collagen. 6. The drug inhibited the platelet aggregation in rat. 7. The drug prolonged the prothrombin time and activated partial thromboplastin time on the test of plasma coagulation factor activity in rat, but was not valuable.

• 한국미생물·생명공학회지
• /
• 제43권2호
• /
• pp.169-174
• /
• 2015

누룩은 전 세계적으로도 독특한 우리만의 발효제로, 전분 당화 및 발효미생물의 접종원으로 이용된다. Rhizopus oryzae KSD-815로 제조된 개량누룩(R4로 명명)은 발효제 특성 이외에 암세포 생육억제, 항비만 활성 등이 알려져 있다. 본 연구에서는 R4 누룩의 열수 및 ethanol 추출물과 이들의 순차적 유기용매 분획물을 조제하여 항혈전 활성을 평가한 결과, ethylacetate 분획에서 우수한 항응고 활성을 확인하였으며, 열수 추출물의 ethylacetate 및 butanol 분획은 인간 적혈구 용혈활성을 나타내지 않으면서 혈소판 응집저해 활성을 나타내었다. 본 연구결과는 R4 누룩이 항응고 및 혈소판 응집저해 개발의 유용자원으로 이용 가능함을 제시하고 있다.

• 동의생리병리학회지
• /
• 제18권5호
• /
• pp.1494-1504
• /
• 2004

The present paper reports the effects of Hwaotang an atherosclerosis using a spontaneous experimental model, We have also investigated the pharmacological effect of Hwaotang on collagen- and ADP-induced blood platelet aggregation, thrombin-induced conversion of fibrinogen and fibrinolysis in in vitro experiments, and various effects on stimuli-induced superoxide generation in human neutrophils. Hwao-tang was shown to have inhibitory effect on collagen- and ADP-induced blood platelet aggregation, on thrombin-induced conversion of fibrinogen to fibrin and on the activity of plasminogen or plasmin. Hwao-tang also significantly inhibited fMLP-induced superoxide generation in a concentration-dependent manner, but not that induced by arachidonic acid. Hwao-tang inhibited neutrophil functions, including degranulation, superoxide generation, and leukotriene B4 production, without any effect on 5-lipoxygenase activity. In conclusion, the protection of extracts of Hwao-tang on the ischemic infarction induced artificially might be involved to their inhibition of thrombotic action. The results also indicate that Hwao-tang exerts the effects on superoxide generation related to the inhibition of neutrophil functions.

• 한국버섯학회지
• /
• 제15권3호
• /
• pp.124-128
• /
• 2017

국내 상황버섯 재배 종인 Phellinus baumii (PB, Jangsu sanghwang), P. linteus (PLK, Korea Sangwhang), 그리고 P. linteus HN00k9 (PLH) 자실체의 methanol 추출물의 혈소판 응집 억제와 ATP release 감소효과를 조사하였다. PB, PLK, PLH methanol 추출물을 전 처리하고 혈소판 응집촉진물인 collagen (2.5 ug/ml), ADP (10 uM), thrombin (0.1 U/ml) 처리에 50ug/ml에서 200 ug/ml 농도에서 50%에서 95%의 혈소판응집억제 효과가 나타났으며 ATP release 감소효과는 200 ug/ml 농도에서 ADP(10 uM) 처리 혈소판에서 60%에서 50%로 나타났으며 이는 Phospholipase C의 inhibitor인 U73122 (4 uM)과 calcium chelator인 EDTA (1 mM)에 비하여 20%이상 높게 나타났다. 본 결과로부터 상황버섯 PB, PLK, PLH 메탄올 추출물은 심혈관계질병을 예방 하는 유용한 소재로 활용 가능 할 것으로 사료 된다.

• Archives of Pharmacal Research
• /
• 제27권4호
• /
• pp.381-385
• /
• 2004

Nine phenolic ($1\~9$) and two furan type (10, 11) compounds, were isolated from the methanolic extract of the tuber of Gastrodia elata Blume (Orchidaceae) in the course of continuing search for platelet anit-aggregating plant components. Compound 1 was identified as 4,4'-dihy-droxybenzyl sulfone, a novel compound for the best of our knowledge. Compound 10, 5-hydroxymethyl-2-furancarboxaldehyde, was isolated for the first time from this plant. Compound 1 ($IC_{50};\;83{\mu}M$) was about four times more inhibitory to U46619 induced aggregation than ASA ($IC_{50};\340{\mu}M$). Compound 9, 4,4'-dihydroxy-dibenzylether, ($IC_{50};\;5{\mu}M$, $3{\mu}M\;and\;33{\mu}M$, respectively) was $10\~}80$ fold more potent than ASA ($IC_{50};\;420\;{\mu}M,\;53\;{\mu}M\;and\;340\;{\mu}M$ respectively) to collagen, epinephrine and U46619 induced aggregation, although it is less active than ASA to AA induced aggregation.