• The Korean Journal of Physiology and Pharmacology
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• 제16권6호
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• pp.463-468
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• 2012

Type 1 diabetes (T1D) is caused by dysregulation of the immune system in the pancreatic islets, which eventually leads to insulin-producing pancreatic ${\beta}$-cell death and destabilization of glucose homeostasis. One of the major characteristics of T1D pathogenesis is the production of inflammatory mediators by macrophages that result in destruction or damage of pancreatic ${\beta}$-cells. In this study the inflammatory microenvironment of T1D was simulated with RAW264.7 cells and MIN6 cells, acting as macrophages and pancreatic ${\beta}$-cells respectably. In this setting, peroxiredoxin-1, an anti-oxidant enzyme was knocked down to observe its functions in the pathogenesis of T1D. RAW264.7 cells were primed with lipopolysaccharide and co-cultured with MIN6 cells while PRX-1 was knocked down in one or both cell types. Our results suggest that hindrance of PRX-1 activity or the deficiency of this enzyme in inflammatory conditions negatively affects pancreatic ${\beta}$-cell survival. The observed decrease in viability of MIN6 cells seems to be caused by nitric oxide production. Additionally, it seems that PRX-1 affects previously reported protective activity of IL-6 in pancreatic ${\beta}$ cells as well. These results signify new, undiscovered roles for PRX-1 in inflammatory conditions and may contribute toward our understanding of autoimmunity.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2003년도 제20차 정기총회 및 학술발표회
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• pp.73-74
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• 2003

육계 병아리에서 어유 함유사료 급여에 의한 급성기 반응중의 생산성 감소 완화와 단백질 대사, 에너지 대사 및 항산화효소 활성의 관계를 조사하였다. 병아리에 1일령부터 어유 사료를 급여하면 급성기 반응중에 에너지 밸런스를 높이나 성장률, 요산 배설량과 질소 밸런스가 낮아지지 않았으며, 대두유 함유 사료를 급여한 것에 비하여 간장과 적혈구 세포액의 SOD 활성을 높였다. 이러한 결과는 어유 함유사료 급여로 급성기 반응중의 증체량 감소가 완화되는 효과는 항산화효소계의 조정과 에너지 급원의 소화관내 흡수 증가에 기인한다는 것을 나타낸다.

• 동의생리병리학회지
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• 제29권3호
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• pp.273-280
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• 2015

In this study, we used the mixture made from the Rice bran 45 ㎏, Dendropanax 5 ㎏, the sugar of the 10% of the total weight, and the enzyme of the 0.1% of the total weight. After the mixture were fermented for 90 days under 20 $^{\circ}C$, we measured the cell viability and the inhibition rate of the melanin biosynthesis, the activity of tyrosinase and superoxide dismutase (SOD) in malignant melanoma, B16F10 cells, in order to survey the whitening effect and the mechanism of the effects on the sample. As a result, the samples significantly suppressed the cell viability of B16F10 in more than 500 ${{\mu}g}$/㎖ and significantly inhibited the generation of melanin induced by ${\alpha}$-MSH in more than 1,000 ${{\mu}g}$/㎖. Sample decreased the activity of tyrosinase while increased the activity of SOD in dose dependent manner. Therefore, we considered that the fermentation extract made from a Rice bran and Dendropanax will be able to produce high value-added products, if used as a commercial.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.473-483
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• 2022

In this study we investigated the effect of lactic acid bacteria (LAB) fermentation on the ingredients and anti-oxidant activity of Withania somnifera extract. Four strains of LAB could proliferate normally in medium containing W. somnifera extract after the pH reached 3.1~3.5. LAB fermentation increased the content of alcohols and ketones, endowing the extract with the characteristic aroma of fermentation. Compared to the control, the DPPH and ABTS free radical scavenging rates in the fermented samples were significantly improved, ranging from 48.5% to 59.6% and 1.2% to 6.4%. The content of total phenols was significantly increased by 36.1% during the fermentation of mixed bacteria. Moreover, the original composition spectrum of the extract was significantly changed while the differentially accumulated metabolites (DAMs) were closely related to bile secretion, tryptophan metabolism and purine metabolism. Therefore, LAB fermentation can be used as a promising way to improve the flavor and bioactivity of the extracts of W. somnifera, making the ferments more attractive for use as functional food.

• 대한융합한의학회지
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• 제1권1호
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• pp.17-24
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• 2021

Objectives: Excessive accumulation of fat on specific region, regarded as localized fat, is one of the serious problems and well-known risk factors of health. Recently, an interest in health and aesthetics is growing by treating lipolytic injection. Polygonatum sibiricum Rehd (PS) has been known to have anti-oxidant, -aging and -atherosclerotic effects. In this study, we investigated the lipolytic effects of PS pharmacopuncture in obese mice. Methods: Male C57BL/6J mice was fed with high fat diet to induce obesity for 12 weeks. PS pharmacopuncture was dissolved in saline by adjusting pH 7. 100 μL of PS pharmacopunture was injected subcutaneously into the left side inguinal fat pad, while saline was injected into the right side inguinal fat pad in mice as self-control. Samples were treated 3 times per weeks for 2 weeks. Results: PS pharmacopunture significantly decreased the inguinal fat weight compared to left side inguinal fat pad. Decrease rate of PS pharmacopuncture was about 21%. In addition, the diameter of adipocyte in inguinal fat tissues was significantly reduced by 17% compared to saline-injected side. There was no sign of toxicity through whole experiments. Conclusion: The present study indiates that PS pharmacopunture could be a material derived from natural herb as a lipolytic injection for decreasing localized fat.

• Animal Bioscience
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• 제37권2호
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• pp.161-172
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• 2024

For sustainable development, better performance, and less gas pollution during rumen fermentation, there is a need to find a green and safe feed additive for ruminants. Cysteamine (CS) is a biological compound naturally produced in mammalian cells. It is widely used as a growth promoter in ruminants because of its ability to control hormone secretions. It mainly controls the circulating concentration of somatostatin and enhances growth hormone production, leading to improved growth performance. CS modulates the rumen fermentation process in a way beneficial for the animals and environment, leading to less methane production and nutrients loss. Another beneficial effect of using CS is that it improves the availability of nutrients to the animals and enhances their absorption. CS also works as an antioxidant and protects the cells from oxidative damage. In addition, CS has no adverse effects on bacterial and fungal alpha diversity in ruminants. Dietary supplementation of CS enhances the population of beneficial microorganisms. Still, no data is available on the use of CS on reproductive performance in ruminants, so there is a need to evaluate the effects of using CS in breeding animals for an extended period. In this review, the action mode of CS was updated according to recently published data to highlight the beneficial effects of using CS in ruminants.

• 농업과학연구
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• 제50권2호
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• pp.219-230
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• 2023

Calla lily is one of the most iconic and widely recognized ornamental plants. This study compared the extracts of 11 cultivars of domestic calla lily bred by the National Institute of Horticultural and Herbal Science for their total polyphenol and antioxidant activities. Eleven cultivars were evaluated for their growth and flowering characteristics as per the Manual for Agricultural Investigation Rural Development Administration (RDA) form. The antioxidant activities were measured using 2,2'-azinobis (3-ethylbebzothiazoloine-6-sulfonic acid)-diammounium salt (ABTS⁺) and 1,1-diphenyl-2-picrrylhydrazyl (DPPH) radical scavenging effect. The plants have an average height of 63.80 ± 5.4 cm, average flower diameter of 7.2 ± 1.1 cm, and width of 12.4 ± 1.7 cm. On average, the diameter and width of leaves were 33.7 ± 3.5 cm and 20.0 ± 1.4 cm, respectively. Extracts of flowers and leaves in the 11 cultivars of white calla lily were compared for their antioxidant activities and total polyphenol contents. ABTS⁺ and DPPH radical scavenging, which are indicative of antioxidant activity, were higher in flowers than in leaves. When comparing by cultivar, we found that 'White Egg' showed the highest antioxidant activity in both the flowers and the leaves. Additionally, we found that by part, the content of total polyphenols was highest in flowers, and by cultivar, it was highest in the 'Swan' and 'White Egg' cultivars. Furthermore, the days to flowering showed correlations with ABTS⁺ radical scavenging, total phenolic contents (TPC), and total flavonoid contents (TFC). Our results indicate that calla lily can be used as breeding material material according to its growth characteristics and as a natural antioxidant source.

• 생명과학회지
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• 제26권1호
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• pp.34-41
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• 2016

학명이 Aronia melanocarpa인 블랙초크베리는 항산화, 항염증, 항암 효능이 뛰어난 것으로 보고되고 있다. 본 연구에서는 블랙초크베리에 대한 collagenase inhibition effects와 산화적 스트레스에 유도된 matrix metalloproteinase(MMP), MAPkinase 그리고 AP-1의 발현 그리고/또는 인산화와 같은 분자생물학적 메카니즘을 조사하였다. Collagenase inhibition 효과는 블랙초크베리 에틸아세테이트 분획물(AE)이 500 μg/ml의 농도에서 77.2% 이상의 저해효능을 나타내었고 이는 대조군인 Epigallocatechin gallate의 결과(500 μg/ml에서 83.9%)와 비교해서 유의할 만한 결과였다. Reactive oxygen species (ROS) assay는 AE에서 가장 농도의존적으로 ROS 생성이 감소되었고, 75 μg/ml의 농도에서 약 70%로 가장 낮은 활성산소가 생성되었다. MTT assay 결과, H₂O₂에 유도된 CCRF 세포에 AE를 처치하였을 때 농도의존적으로 세포 생존율이 증가하였다. 그리고 특히, AE는 H₂O₂에 유도된 CCRF 세포에서의 MMPs (MMP-1, -3 그리고 -9), MAPK (ERK, JNK 그리고 p38) 그리고 AP-1 (c-Fos와 c-Jun)의 발현과 인산화를 억제하였고, pro-collagen type I의 발현은 증가시켰다. 따라서 블랙초크베리 에틸아세테이트 분획물은 주름억제 및 콜라겐 생성의 효능이 있으며 기능성 식품 및 화장품 소재 개발 산업에서의 응용이 가능할 것으로 기대된다.

• 생명과학회지
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• 제32권12호
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• pp.979-988
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• 2022

비만은 당뇨, 고혈압, 심근경색 등 심혈관 질환을 유발하는 주요 원인으로 이와 관련된 질환의 발병률이 증가하고 있다. 이로 인해 비만 치료 및 예방에 대한 관심이 급증하는 추세이며, 비만 치료제가 전 세계적으로 개발되고 있지만 많은 부작용을 초래하여 시판이 중단되거나 장기복용이 금지되어 있다. 이러한 문제를 막기 위해 부작용이 적으면서 항비만 효과를 가지는 천연 소재를 활용한 연구가 진행되고 있다. 본 연구에서 사용된 해당화(Rosa rugosa)는 장미과에 속하는 낙엽활엽관목으로서 토혈, 하리, 월경과다 등에 이용되고 있으며, 항염증, 항산화 효과, 노화방지, 혈당 강하작용, 혈청 콜레스테롤 수치 저하작용, HIV protease 저해 등에도 효과가 있는 것으로 알려져 있다. 따라서 본 연구에서는 해당화 추출물 및 분획물 4종(H₂O, n-BuOH, 85% aq. MeOH, n-Hex)의 지방세포분화에 미치는 효과에 대한 연구를 진행하였다. 3T3-L1 지방전구세포를 지방세포로의 분화를 유도하여 Oil-Red O staining으로 지방생성 억제 정도를 측정하였다. 그 결과, 갯완두 추출물 및 분획물 처리군에서 농도 의존적으로 지방세포 형성이 억제되는 것을 확인하였다. 또한 지방세포분화에 관여하는 PPARγ, C/EBPα 및 SREBP1c와 같은 인자의 mRNA 및 단백질 발현에 미치는 효과를 확인한 결과, 지방세포분화 조절인자의 발현이 농도 의존적으로 감소하는 것을 확인하였다. 이는 AMPK 신호전달경로를 통한 지방분화인자의 억제를 통한 것으로 확인되었다. 따라서 본 연구결과로부터 해당화 추출물 및 분획물의 항비만 효과를 확인하였으며, 나아가 비만 치료제의 소재로 개발될 수 있는 가능성을 제시할 것으로 기대된다.