• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1412-1419
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• 2020

Human noroviruses (HuNoVs) are a leading cause of gastroenteritis outbreaks worldwide. However, the paucity of appropriate cell culture models for HuNoV replication has prevented developing effective anti-HuNoV therapies. In this study, first, the replication of the virus at various temperatures in different cells was compared, which showed that lowering the culture temperature from 37℃ significantly increased virus replication in Madin-Darby canine kidney (MDCK) cells. Second, the expression levels of autophagy-, immune-, and apoptosis-related genes at 30℃ and 37℃ were compared to explore factors affecting HuNoV replication. HuNoV cultured at 37℃ showed significantly increased autophagy-related genes (ATG5 and ATG7) and immune-related genes (IFNA, IFNB, ISG15, and NFKB) compared to mock. However, the virus cultured at 30℃ showed significantly decreased expression of autophagy-related genes (ATG5 and ATG7), but not significantly different major immune-related genes (IFNA, ISG15, and NFKB) compared to mock. Importantly, expression of the transcription factor FOXO1, which controls autophagy- and immune-related gene expression, was significantly lower at 30℃. Moreover, FOXO1 inhibition in temperature-optimized MDCK cells enhanced HuNoV replication, highlighting FOXO1 inhibition as an approach for successful virus replication. In the temperature-optimized cells, various HuNoV genotypes were successfully replicated, with GI.8 showing the highest replication levels followed by GII.1, GII.3, and GII.4. Furthermore, ultrastructural analysis of the infected cells revealed functional HuNoV replication at low temperature, with increased cellular apoptosis and decreased autophagic vacuoles. In conclusion, temperature-optimized MDCK cells can be used as a convenient culture model for HuNoV replication by inhibiting FOXO1 and providing adaptability to different genotypes.

• Journal of the Korean Society of Industry Convergence
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• v.23 no.5
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• pp.757-763
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• 2020

City gas buried pipes are managed by corrosion protection to prevent corrosion. In the case of the press-in section, the double pipe and the main pipe may cause corrosion under the influence of stray current, which can shorten the life of the pipes. In addition, if the insulator is filled in the press-in section, the press-in section itself is a single structure, and can be directly affected by external impact, and when the surrounding ground subsidence occurs, the stress may be concentrated, resulting in serious consequences. In this study, a serration-type shock absorber in the form of a sliding support was proposed as a new buried double piping construction method using EPS. The serration-type shock absorber can contribute to the improvement of the integrity of the buried double piping, as it can utilize the gas piping's own ductility and stress distribution characteristics with proper anti-corrosion management and shock-absorbing material properties by preventing contact inside the buried double pipe. However, for application to ground piping, there remains a task to supplement the vulnerability against fire due to the characteristics of EPS materials.

• Geomechanics and Engineering
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• v.11 no.4
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• pp.457-469
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• 2016

Steep rock slope with water-filled tension crack will happen to overturn around the toe of the slope under seismic loading. This failure type is completely different from the common toppling failure occurring in anti-dipping layered rock mass slopes with steeply dipping discontinuities. This paper presents an analytical approach to determine the seismic factor of safety against overturning for an intact rock mass slope with water-filled tension crack considering horizontal and vertical seismic coefficients. This solution is a generalized explicit expression and is derived using the moment equilibrium approach. A numerical program based on discontinuous deformation analysis (DDA) is adopted to validate the analytical results. The parametric study is carried out to adequately investigate the effect of horizontal and vertical seismic coefficients on the overall stability against overturning for a saturated rock slope under two water pressure modes. The analytical results show that vertically upward seismic inertia force or/and second water pressure distribution mode will remarkably decrease the slope stability against overturning. Finally, several representative design charts of slopes also are presented for the practical application.

• Journal of the Korea Safety Management & Science
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• v.16 no.3
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• pp.55-62
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• 2014

During five years (Year 2009~2013), Total victims of 72 %(81,560 people) and those 50.6 %(1,258 people) of death accident occurs in small scale construction site which operate 2 million USD less construction budget. Especially, falling death accident account for 785 people, in the share of 33.2 %(261 people) death disaster takes due to defect of original cause materials. The major safety issues in small scale work place take place while scaffold installation, disassembling, work-plate improper installation or non-professional skills of workers. Furthermore, labor subcontract systems make small construction site shortage of resources. Those workers regard work-plate as unnecessary and consumables supplies. Because of that most of workers use unsafe workplace in most construction site. Therefore, in order to prevent falling accident occurring in small scale work site, government should organize related regulations such as "Work site safety construction method" and then expands education support, financial aid, and sourcing safety supplies for work plate which offer broad variety experiences. Also, introduce certificate solutions for various work plates to improve safety function such as anchoring type method and anti sliding function.

• Geomechanics and Engineering
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• v.32 no.6
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• pp.639-652
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• 2023

Stepped earth slopes incorporated with anti-slide piles are widely utilized in landslide disaster preventions. Explicit consideration of the three-dimensional (3D) effect in the slope design warrants producing more realistic solutions. A 3D limit analysis of the stability of pile stabilized stepped slopes is performed in light of the kinematic limit analysis theorem. The influences of seismic excitation and surcharge load are both considered from a kinematic perspective. The upper bound solution to the factor of safety is optimized and compared with published solutions, demonstrating the capability and applicability of the proposed method. Comparative studies are performed with respect to the roles of 3D effect, pile location, pile spacing, seismic and surcharge loads in the safety assessments of stepped slopes. The results demonstrate that the stability of pile reinforced stepped slopes differ with that of single stage slopes dramatically. The optimum pile location lies in the upper portion of the slope around L_x/L = 0.9, but may also lies in the shoulder of the bench. The pile reinforcement reaches 10% universally for a looser pile spacing D_c/d_p = 5.0, and approaches 70% when the pile spacing reaches D_c/d_p = 2.0.

• Fire Science and Engineering
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• v.26 no.3
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• pp.106-111
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• 2012

In this study, EIFS (Exterior insulation finish system) of exterior cladding was applied Cone calorimeter test to confirm the effect of flame retardant. As a results, the initial ignition points in accordance with the coated form and concentration of the flame retardant was delayed. But flame resistant treatment of EIFS cladding to control the fire will not affect confirmed that. In addition, EIFS that uses high-density and low-density due to difference in the density of the impact of the fire was no difference. The exterior of the ignition experiment occurred before and after 40 seconds, heat release rate to 100 seconds appears to occur about 90 % compared with the other exterior wall materials, the initial fire spread very fast was confirmed. EIFS cladding in order to prevent the spread of fire in the application of EIFS legally use is limited by the use of the building. And flame spread can be prevented, such as a vertical outer wall compartment measures are urgently needed.

• Journal of Korean Society of societal Security
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• v.3 no.2
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• pp.33-39
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• 2010

This study is to reduce the rate of drowning accident by using of buoyancy bag. People are exposed to the high risk of drowning accident when they have water leisure activities due to the lack of safety mind-set and shortage of safety products. In case of drowning accident, the rescue action is normally depending on the other people's assistance. Therefore, rescue activities which relyn the people doesn't improve the ratio of survival in the drowning accidents. The submarine specialists should use the buoyancy products to rescue the people in the drowning accident. The citizen can carry portable buoyancy product in the automobile as well as by hands anywhere and anytime. It will be effective rescue tool to save his/her life in the emergency. In addition, it will contribute to rescue other's life because it can be used immediately. There are 3 positive characteristics on the buoyancy bag. First, it is convenience. Documents and other stuffs can be kept in the bag. Second is safety. The material of buoyancy is placed in the inner of the bag. It is possible to float the person whose weight is 90 kg. Lastly, it is durability. It can be used long-term because the outer of the bag is made of anti-water material and anti-water zipper. As a result of performance analysis, it is evident that the buoyancy bag can be used to rescue the people in the drowning accident as a indirect rescue tool compared with the current other rescue products. It is recommended that the design of outer box and performance of buoyancy are required to be improved in order to contribute more to rescue people in the accident.

• Fire Science and Engineering
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• v.30 no.4
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• pp.27-38
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• 2016

The paper reports the development of a distributed temperature sensing (DTS) system, which is a fire detection system using optical sensor linear detectors that depends on foreign and domestic technologies. This study accordingly analyzed the electrical signal patterns of Raman scattering light mainly used for temperature sensing among back-scattered light generated in optical fiber by using an oscilloscope. Through the measurement results, it could be verified that the Stokes signal patterns had little change by the temperature increase, but the temperature-sensitive anti-Stokes patterns had relative increase of the changes. This study developed a K-DTS system, which is an optical sensor linear fire detection system composed of an optical repeater and a receiver that can detect fires using Raman scattering light. It could be verified that the developed K-DTS system satisfied the type approval standards through the sensitivity tests using the rate of rise type and fixed temperature type sensitivity testers. In addition, performance experiments have been performed for performance evaluation of the K-DTS system developed in comparison with S-DTS system which has been imported from abroad and widely used in Korea. It can be confirmed from the results of the performance experiments using model tunnels that comparable performances can be obtained in fire detection locations and the measurements of fire temperatures.

• Journal of Bio-Environment Control
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• v.28 no.2
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• pp.150-157
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• 2019

This study was carried out to investigate at the micrometeorology change of the orchard according to the net installation. Two weather stations were installed at the inside of the anti-insect nets(2 mm, 4 mm), one was installed at the outside(control). They were observed the temperature, humidity, wind speed and solar radiation from April to September 2018. Daily mean temperature at the experimental group was higher than control group by $0.2^{\circ}C$. Daily mean humidity at the experimental group was higher than control group by 3.5 to 4.7%. Daily mean the solar radiation at the experimental group(2 mm) was lower than control group by 50%. The wind speed was decreased from 12% to 50% of the external wind speed at 4 mm, and from 25% to 59% at 2 mm.

• Safety and Health at Work
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• v.5 no.3
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• pp.131-135
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• 2014

Background: Cassava and corn milling is a growing small-scale enterprise in Africa. We aimed to determine the incidence of hand-arm musculoskeletal complaints among vibration-exposed Congolese cassava and corn millers in the previous 12 months. Methods: A cross-sectional study was conducted, prior to a follow-up study, from March to May 2013 among cassava/corn millers in Lubumbashi, Democratic Republic of Congo, in which 365 millers age-matched to 365 civil workers anonymously answered a questionnaire. Results: Overall incidence of hand-arm musculoskeletal complaints was 25.8% in millers (vs. 5.2% in civil workers; p < 0.001). The risk of experiencing musculoskeletal symptoms was seven times higher in millers [vs. civil workers; odds ratio (OR) = 7.10; 95% confidence interval (CI): 4.03-12.50; p < 0.0001]; 2.4 times higher in smoking millers (vs. smoking civil office workers; OR = 2.36; 95% CI: 1.42-3.88; p < 0.001); 3.6 times higher in millers with longer daily exposure (> 8 hours; vs. those working ${\leq}8$ hours; OR = 3.56; 95% CI: 1.93-3.61; p = 0.026); and 7.4 times higher in young millers (vs. older millers, OR = 7.39; 95% CI: 1.29-75.52; p < 0.001). Smoking, number of cigarettes, and daily exposure duration were positively correlated with musculoskeletal complaints. Conclusion: This study revealed a relatively high incidence of musculoskeletal complaints among African cassava and corn millers. The use of anti-vibration protective equipment and the regulation of this hazardous occupation may reduce the burden of musculoskeletal disorders in millers.