• Advances in Traditional Medicine
• /
• 제7권5호
• /
• pp.540-548
• /
• 2008

Paeonia radix is the root of Paeonia aliflora Pallas, which is a perennial plant classified in the family Paeoniaceae. Paeonia radix possesses several pharmacological effects such as analgesic, anti-inflammatory and anti-allergic, anti-oxidative, and anti-coagulant activities. In this study, we investigated the effect of the aqueous extract of Paeonia radix on the lipopolysaccharide-induced inflammation in mouse BV2 microglial cells. The aqueous extract of Paeonia radix at respective concentration was treated one hour before lipopolysaccharide treatment. In the present results, the aqueous extract of Paeonia radix suppressed prostaglandin $E_2$ synthesis and nitric oxide production by inhibiting the lipopolysaccharide-stimulated mRNA expressions of cyclooxygenase-2 and inducible nitric oxide synthase in mouse BV2 microglial cells. These results demonstrate that Paeonia radix exerts anti-inflammatory and analgesic effects probably by suppressing mRNA expressions of cyclooxygenase-2 and inducible nitric oxide synthesis. The present study demonstrates that Paeonia radix may offer a valuable mean of therapy for brain inflammatory diseases.

• 한국식품영양과학회지
• /
• 제36권2호
• /
• pp.155-162
• /
• 2007

본 연구에서는 녹차(Camellia sinensis)잎 추출물, 구아바(Psidium guajava)잎 추출물 그리고 장미(Rosa hybrida)꽃잎 추출물로 이루어진 식물추출 복합물(PEM381)에 대하여 제I형 알레르기 반응 억제 효능 및 생화학적 기전을 밝히기 위하여 COX 억제 활성, LO 억제 활성, compound 48/80에 의한 랫드 복강 비만세포의 탈과립과 히스타민 유리에 미치는 영향 그리고 수동피부아나필락시스 반응 억제효능을 측정하였다. 그 결과 PEM381은 5-LO $IC_{50}$ 값이 $14.11{\pm}0.51ppm$으로 나타났고, COX-1에 비해 COX-2를 선택적으로 억제하는 것으로 나타났다. 또한 PEM381은 compound 48/80에 의한 비만세포의 탈과립과 히스타민 유리를 농도 의존적으로 억제할 뿐만 아니라 수동피부아나필락시스 반응도 억제하였다. 이상의 결과로 PEM381은 알레르기성 비염, 아토피 피부염 및 기관지천식 같은 제I형 알레르기 질환에 대하여 예방 및 치료에 유용하게 사용될 수 있을 것으로 생각된다.

• 한국응용과학기술학회지
• /
• 제35권3호
• /
• pp.975-984
• /
• 2018

마치현 추출물에 대한 생리활성 소재로서 응용 가능성을 확인하고자 하였다. 마치현 추출물의 측정 결과, 플라보노이드, 폴리페놀 함량과 DPPH radical 소거능을 확인하였으며, 세포실험 결과 HaCaT, RAW 264.7, RBL-2H3 세포에서 유의한 세포독성은 나타나지 않았으며. $H_2O_2$에 의한 유발되는 산화적 스트레스에 대한 HaCaT 세포는 마치현 에탄올 $100{\mu}g/mL$ 농도에서 83% 보호효과가 확인되었다. RAW 264.7 세포에 대한 마치현 추출물의 항염 효과를 확인 결과 저농도에서도 nitric oxide 생성이 억제되었으며, S. aureus, S. epidermidis, P. acnes 균에서도 마치현 추출물의 농도 의존적으로 항균 활성이 확인되었다. 본 연구는 마치현 추출물의 항산화, 항염증 및 항균 효과를 가지는 생리 활성 물질로서 활용 가능성을 확인하였다.

• Natural Product Sciences
• /
• 제20권4호
• /
• pp.251-257
• /
• 2014

Chungsimyeonja-tang (CSYJT) is an herbal decoction that consists with 12 herbal medicines. CSYJT has been shown to have anti-stress, anti-allergic and anti-oxidant effects. The aim of this study was to determine flavonoid contents in CSYJT and evaluate its protective effect against cisplatin induced nephrotoxicity using both in vitro (porcine renal epithelial cell; PK15 cell) and in vivo (Sprague Dawley rat) experiments. In the present study, thee mean contents of baicalin, wogonoside and baicalein in CSYJT were 14.65, 5.27 and 0.02 mg/g, respectively. The CSYJT extract treatment attenuated the following alteration in porcine renal epithelial (PK15) cell: the increase in reactive oxygen species (ROS), the glutathione depletion and the increase in p53 expression induced by cisplatin treatment. In the in vivo study, rats were orally treated with CSYJT extract once a day for 28 days. Five days before the last treatment, cisplatin (5 mg/kg) was intraperitoneally injected to induce acute renal failure. Increased blood urea nitrogen (BUN) and creatinine (CRE) levels after cisplatin treatment were ameliorated by pretreatment of CSYJT extract. In addition, lipid peroxidation was decreased and antioxidant enzyme (glutathione) was recovered in CSYJT pretreated kidney tissue. In histopathological examination, CSYJT pretreated group showed ameliorated pathological alteration after cisplatin injection with decreased apoptosis. Taken together, pretreatment of CSYJT could ameliorate cisplatin-induced nephrotoxicity.

• 한국식품영양학회지
• /
• 제19권2호
• /
• pp.201-206
• /
• 2006

Numerous investigators have studied various activities of natural products and have found that they have not only nutritional effects, but also beneficial properties to cure various diseases and to maintain good health. Job's Tear(Yul-Moo) is a grass crop that has long been used in traditional medicine and as a nourishing food. Although its mechanism of action remains unclear, Job's Tear has been reported to exhibit anti-inflammatory, stomachic, anti-allergic, and anti-spastic effects and has been used in China for the treatment of warts, rheumatism, and neuralgia. Previous results in our laboratory demonstrated that the ethanol extract and the water extract of Job's Tear exerted an immune regulatory function on mice cells in vitro. The present study was performed to investigate the ex vivo effect of Job's Tear on immune function. Seven to eight weeks old mice(Balb/c) were fed chow diet ad libitum and water extract of Job's Tear was administered orally every other day for four weeks at two different concentrations(50 and 500mg/kg B.W.). Splenocytes proliferation with mitogen stimulation with Con A and LPS was enhanced at 50 mg/kg B.W. of Job's Tear compared to those of the control group. The results of this ex vivo study showed that proliferation of splenocytes and macrophage activation were seen in the mice orally administrated 50 mg/kg B.W. of Job's Tear water extracts. In conclusion, this study suggests that Job's Tear extracts may enhance immune function by regulating splenocyte proliferation and the cytokine prodution capacity of activated macrophages in mice.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
• /
• pp.99-99
• /
• 2003

We studied the effect of Rubus croceacanthus Leveille (RCL) on mast cell-mediated anaphylactic reactions. RCL inhibited compound 48/80-induced systemic anaphylactic shock. When RCL was given at concentrations ranging from 0.01 to 1 mg/$m\ell$, the histamine release from rat peritoneal mast cells induced by compound 48/80 was reduced in a dose-dependent manner. RCL also inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl IgE. In addition, RCL inhibited phorbol 12-myristate 13-acetate and A23187-induced tumor necrosis factor-${\alpha}$ secretion from human mast cell line HMC-1 cells. These results indicate that RCL may possess a strong anti-anaphylactic activity.

• 대한본초학회지
• /
• 제28권2호
• /
• pp.55-60
• /
• 2013

Objectives : Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by eczematous inflammtion of the skin. The chestnut inner shell extracts (CI) has been used as a cosmetic material for a long time in Korea. However, the precise anti-allergy effects of CI have yet to be clearly elucidated. In this study, we attempted to evaluate the effect of CI on mast cell-mediated allergy inflammation. Methods : To find the anti-allergy and inflammatory effect of CI, we investigated the inhibitory effect of CI on the production of inflammatory mediators using by enzyme-linked immunosorbent assay in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore (A23187) stimulated-human mast cell (HMC-1). Results : In this study, we found that CI did not show cytotoxic effect at up to 10 ug/ml on HMC-1. CI inhibited the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6 and IL-8 in stimulated HMC-1. Maximal rate of TNF-${\alpha}$, IL-6 and IL-8 inhibition by CI (10 ug/ml) were about 47.6%, 44.1% and 22.5% respectively. In addition, we showed that Fr.3 isolated from n-Butyl alcohol layer of CI attenuated the production of TNF-${\alpha}$, IL-6 and IL-8 in HMC-1. Conclusion : Taken together, the findings of this study provide us with a novel insight action of CI as a potential molecule for use in the treatment of allergic inflammation diseases.

• 생명과학회지
• /
• 제13권1호
• /
• pp.73-82
• /
• 2003

보중익기탕이 즉시형 및 지연형 알레르기에 대한 면역 반응에 미치는 영향을 알아보고자 항 알레르기에 대한 실험을 즉시형과 지연형으로 구분하여 시행하여 다음과 같은 결론을 얻었다 Compound 48/80 유발 아나필락시스 반응에 미치는 영향은 농도별 실험에서 보중익기탕 투여군은 생존율이 비순차적이었으나 생존율이 증가하였다. 또한 시간의존적 실험에서 보중익기탕 투여군은 다소의 생존율 변화를 보였으며, Compound 48/80 유발 비만세포 탈 과립 율의 측정을 통하여 대조군에 비해 보중익기탕 투여군은 현저하게 유의한 (p<0.005) 감소효과가 인정되었다. Picryl chloride에 의한 접촉성 피부염증 반응에 미치는 효과에 관한 실험과 면양적혈구에 의한 지연형 족부종 반응에 미치는 효과에 관한 실험에서 대조군에 비하여 보중익기탕 투여군은 유의한 억제효과가 인정되었다. 이러한 실험결과에서 보중익기탕이 즉시형 및 지연형 알레르기반응에 있어 모두 효과를 발휘하는 것으로 나타났으며 보중익기탕의 항 알레르기 효과를 질병의 치료에 응용할 수 있을 것으로 생각된다. 또한 향후 지속적인 실험과 연구를 통해 임상적 치료와 활용에 발전이 있을 것이라 사료된다.

• Journal of Microbiology and Biotechnology
• /
• 제23권11호
• /
• pp.1636-1640
• /
• 2013

We had found that orally administered Lactobacillus species were effective immune modulators in ovalbumin (OVA)-sensitized mice. To validate these findings, we investigated the effects of orally administered Lactobacillus brevis HY7401 in OVA-T cell receptor transgenic mice. This strain showed a tendency to induce Th1 cytokines and inhibit Th2 cytokines. All assayed isotypes of OVA-specific antibody were effectively reduced. Systemic anaphylaxis was also relatively reduced with the probiotic administration. These results reveal that L. brevis HY7401 might be useful to promote anti-allergic processes through oral administration.

• 약학회지
• /
• 제47권5호
• /
• pp.325-330
• /
• 2003

Solanum melongena L. (Solanaceae) has anti-oxidant, analgesic, and hypolipidemic effects. We previously showed that Solanum melongena (SM) aqueous extract inhibits mast cell-mediated allergic reactions. The activation of protease-activated receptor-2 (PAR-2) induces acute inflammation in rat hindpaw. In the present study, we investigated the effects of the SM aqueous extract on mouse paw edema induced by PAR2 agonists. Trypsin or trans-cinnamoyl-LIGRLO-NH$_2$ (tc-NH$_2$), PAR-2 agonists, was injected into the hind paw of mice to induce paw edema. SM aqueous extract (1, 5, 10, and 100 mg/kg) was orally administered 1 hr before induction of paw edema. SM aqueous extract (5, 10, and 100 mg/kg) significantly inhibited both paw edema and vascular permeability in the dose-dependent manner. Furthermore, SM aqueous extract (10 mg/kg) significantly inhibited PAR-2 agonist-induced myeloperoxidase (MPO) activity and tumor necrosis factor (TNF)-$\alpha$ expression in paw tissue compared to that of saline. These results suggest that SM aqueous extract may be useful for treatment of PAR-2-mediated inflammation.