The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지

Effect of Job's Tear(Yul-Moo) Extracts on Mouse Spleen and $IL-1{\beta},\;IL-6,\;TNF-{\alpha}$ Cytokine Production by Peritoneal Macrophages

4주 동안의 율무 추출물 투여가 사이토카인 $IL-1{\beta},\;IL-6,\;TNF-{\alpha}$ 생성과 비장세포 증식에 미치는 영향

  • Ryu Hye-Sook (Department of Food and Nutrition, Sangji University) ;
  • Kim Hyun-Sook (Major in Food & Nutrition, Sookmyung Women's University)
  • 류혜숙 (상지대학교 이공대학 식품영양학과) ;
  • 김현숙 (숙명여자대학교 생활과학대학 식품영양학과)
  • Published : 2006.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Numerous investigators have studied various activities of natural products and have found that they have not only nutritional effects, but also beneficial properties to cure various diseases and to maintain good health. Job's Tear(Yul-Moo) is a grass crop that has long been used in traditional medicine and as a nourishing food. Although its mechanism of action remains unclear, Job's Tear has been reported to exhibit anti-inflammatory, stomachic, anti-allergic, and anti-spastic effects and has been used in China for the treatment of warts, rheumatism, and neuralgia. Previous results in our laboratory demonstrated that the ethanol extract and the water extract of Job's Tear exerted an immune regulatory function on mice cells in vitro. The present study was performed to investigate the ex vivo effect of Job's Tear on immune function. Seven to eight weeks old mice(Balb/c) were fed chow diet ad libitum and water extract of Job's Tear was administered orally every other day for four weeks at two different concentrations(50 and 500mg/kg B.W.). Splenocytes proliferation with mitogen stimulation with Con A and LPS was enhanced at 50 mg/kg B.W. of Job's Tear compared to those of the control group. The results of this ex vivo study showed that proliferation of splenocytes and macrophage activation were seen in the mice orally administrated 50 mg/kg B.W. of Job's Tear water extracts. In conclusion, this study suggests that Job's Tear extracts may enhance immune function by regulating splenocyte proliferation and the cytokine prodution capacity of activated macrophages in mice.

Keywords

References

  1. Wagner, H. Search for plant derived natural products with immunostimulatory activity. Pure & Appl. Chem. 66(7):1271. 1990
  2. Lee, JS, Shin, YJ, Lim, HJ, Choi, WE and Lee, YK. Antitumor and antimutagenic effect of protien polysaccarides from Polyporus umbellatus. J. Korean Soc. Food. Sci. Nutr. 33:475-470. 2004 https://doi.org/10.3746/jkfn.2004.33.3.475
  3. Pya, MY and Su, MH . Effects of Phellinus linteus extracts on the humoral immune response in normal and cyclophosphamide-treated mice. Journal of Applied Phamcology 9:194-200. 2001
  4. Park, JS and Chyun, JH. Effects of low fat diet and saturated fat supplementation on the immune status of BALB/c mouse. Korean J. Nutrition 26(5):578-585. 1993
  5. Yoo, TJ. Sikpoombogam. Moonundang, Seoul, 288-290. 1998
  6. Tanimura, A. Studies on the anti-tumor component in the seeds of Coix lachryma jobi L. var. ma-yuen Stapf II. The structure of coixenolide, Chem. Phar. Bull. 9: 47-53. 1961 https://doi.org/10.1248/cpb.9.47
  7. Numata, MA, Yamamoto, M and Yamada, H. Antitumor components isolated from the Chinese herbal medicine Coix lachryma-jabi L. Planta Med. 60 : 356-359. 1994 https://doi.org/10.1055/s-2006-959500
  8. Kond, YK, Nonno, C and Hikino, H. Isolation of ovulatory-active substances from crops of Job's tears (Coix lachryma jobi L. var. ma-yuen Stapf). Che. Pharm Bull. 36 : 3147-3152. 1998
  9. Takahashi, M and Konno, CH. Isolation and hypoglycemic activity of coixans A, B and C gly¬cans of Coix lachryma-jobi L. var. ma-yuen Stapf seeds. Planta Med. Fed. 1: 64-65. 1986
  10. Yamada, H, Yanahira, S, Kiyohara, H, Cyong, JC and Otsuka, Y. Water-soluble glucans from the seed of Coix lachryma-jobi L. var. ma-yuen seeds. Phytochem. 25: 129-132. 1986
  11. Ary, ME, Shewry, PR and Richardson, M. The amino acid sequence of a cereal Bowman-Birk type trypsin inhibitor from seeds of job's tear. FEBS Lett. 229: 111-118. 1988 https://doi.org/10.1016/0014-5793(88)80808-1
  12. Park, Y, Suzuki, H and Lee, YS. Effect of coxi on plasma, liver and fecal lipid components in the rat fed on lard or soybean oil-cholesterol diet. Biochem. Med. Metab. Biol. 39 : 7-1. 1988
  13. Ary, MB, Richardson, M and Shewry, PR. Purification and characterization of an insect alpha-amylase inhibitor/endochitinase from seeds of Job's tears Biochim. Biophys. Acta 999:260-266. 1988
  14. Mishell, BB and Shigi, SM. Selected methods in cellular immunology. 1st ed. San Francisco. WH Freeman and Co. 4. 1980
  15. Choi, SE. Effect of needle extracts on mouse splenocyte proliferation and cytokine production by activated mouse peritoneal macrophage. Master's thesis. Sookmyung Women's University. 2000
  16. Lee, YS. Effect of bark Extract on Modulation of Immunocompetence and Irradiation-induced Inflammation Response in Mice. Ph.D. Dissertation Sookmyung Women's University. 2003
  17. Moon, EY, Park, SY and Park, EK. Influence of Angelicae Gigantis Radix on the immune system. Kor. J. of Immunol. 13 : 191. 1991
  18. Wichmann, MW, Ayala, A and Chaudry, IH. Male sex steroid are responnsble for macropage immune function after trauma-hemorrhage, The American Physiological Society, C1335-C1340. 1997