• 대한물리치료과학회지
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• 제10권1호
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• pp.170-179
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• 2003

This study was performed that how phonophoresis using ultrasound for piroxicam affects transdermal permeation and anti-inflammative effects. Transdermal permeation study conducted by using hairless mouse had two categories: control group and ultrasound group. Transdermal permeation was observed according to duty cycle and intensity. Anti-inflammatory effects were determined using in Sprague-Dawley rat. The subjects were divided into three groups of six SD rat each 24 hour, 48 hour, 72 hour. The results of this study were as follows: 1. Transdermal permeation of piroxicam was measured according to ultrasound duty cycle. This research demonstrates that ultrasound group retains more transdermal permeation than control group, and that pulsed ultrasound group holds a little more transdermal permeation than continuous ultrasound group. 2. The transdermal permeation of piroxicam is closely related with ultrasound intensity. Effect of each group of transdermal permeation was significant rises in proportion to ultrasound intensity. 3. By observing inflammation of the tissue caused by trauma, phonophoresis group showed more significant of anti-inflammatory effect. The conclusion of phonophoresis was found to improve significantly the transdermal permeation and the anti-inflammatory effect.

• 생명과학회지
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• 제26권7호
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• pp.772-781
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• 2016

쇠똥구리는 동물의 분을 이용하여 토질을 개선시켜 지구 생태계에 매우 중요한 역할을 담당하는 곤충이다. 이 연구에서 수컷 SD rat는 5군으로 분리되어 PBS, 쇠똥구리 에탄올 추출물, 쇠똥구리 아세톤 추출물, 귀뚜라미 아세톤 추출물, 양성대조군으로 새우기름 그룹으로 나누었다. 13주령 쥐에 고지방식이로 비만을 7주간 유도하고, 20주령부터 시험물질을 고지방 식이를 하면서 한 달간 투여하였다. 쇠똥구리 추출물의 투여는 체중과 복부지방과 부고환지방 중량의 감소로 이어졌다. 지질의 산화적 스트레스는 간에서 MDA를 측정하여 평가하였으나, 군간에 유 의성이 없었다. 단백질의 산화적 스트레스는 혈액에서 단백질 카르보닐 양으로 측정되었는데 쇠똥구리 에탄올 추출물과 쇠똥구리 아세톤 추출물에서 유의성있게 산화적 스트레스가 감소되었다. 반면에 간에서의 단백질 카르보닐 양은 군간에 유의성이 없었다. 당뇨 혈관내피세포를 효소면역분석법을 사용하여 세포부착단백질인 laminin 과 fibronectin 수준을 측정하였더니 쇠똥구리 추출물에서 유의성이 있었다. 사이토카인 IL-10, IL-1β, VEGF, eNOS를 측정하였는데 IL-10에서 쇠똥구리 에탄올 추출물과 쇠똥구리 아세톤 추출물에서 유의성이 있었다. 항산 화효소인 SOD, GSH-Px 활성은 군간에 유의성은 없었지만, 증가하는 경향이 있었고, CAT활성은 쇠똥구리 추출물에서 유의성있게 증가되었다. 지방조직 내의 포화지방산 비율이 감소하는 경향이 나타났으며, 불포화지방산과 다 가불포화지방산은 증가하였다. 쇠똥구리 추출물의 지방조직 감소 및 여러 지질 수치의 향상효과는 쇠똥구리 추출물을 기능성 곤충 소재로 이용할 수 있는 가능성을 제시한다.

• 대한약침학회지
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• 제17권2호
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• pp.7-17
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• 2014

Objectives: Condurango is widely used in various systems of complementary and alternative medicines (CAM) against oesophageal and stomach ailments including certain types of cancer. However, until now no systematic study has been conducted to verify its efficacy and dose with proper experimental support. Therefore, we examined if ethanolic extract of Condurango could ameliorate benzo[a]pyrene (BaP)-induced lung cancer in rats, in vivo to validate its use as traditional medicine. Methods: Fifteen male and 15 female Sprague-Dawley (SD) rats were treated with 0.28 mg/kg of Sweet Bee Venom (SBV) (high-dosage group) and the same numbers of male and female SD rats were treated with 0.2 mL/kg of normal saline (control group) for 13 weeks. We selected five male and five female SD rats from the high-dosage group and the same numbers of male and female SD rats from the control group, and we observed these rats for four weeks. We conducted body-weight measurements, ophthalmic examinations, urinalyses and hematology, biochemistry, histology tests. Results: A histological study revealed gradual progress in lung tissue-repair activity in Condurango-fed cancer-bearing rats, showing gradual tissue recovery after three months of drug administration. Condurango has the capacity to generate reactive oxygen species (ROS), which may contribute to a reduction in anti-oxidative activity and to an induction of oxidative stress-mediated cancer cell-death. Condurango-activated pro-apoptotic genes (Bax, caspase-3, caspase-9, p53, cytochrome-c, apaf-1, ICAD and PARP) and down-regulated antiapoptotic-Bcl-2 expression were noted both at mRNA and protein levels. Studies on caspase-3 activation and PARP cleavage by western blot analysis revealed that Condurango induced apoptosis through a caspase-3-dependent pathway. Conclusion: The anticancer efficacy of an ethanolic extract of Condurango for treating BaP-induced lung cancer in rats lends support for its use in various traditional systems of medicine.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.155.1-155.1
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• 2003

In the present study, the effect of DW-1350, a newly synthesized anti-osteoporotic agent, was evaluated in ovariectomized Rat. Female SD Rat mice underwent bilateral ovariectomy for prevention study that test article was administered from 2 days after ovariectomy for 6 weeks, for therapeutic study it was conducted from 6 weeks after ovariectomy for three months. (omitted)

• 한방안이비인후피부과학회지
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• 제15권1호
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• pp.31-49
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• 2002

This Experimental study was done to research effects of Fagopyrum esculentum Moench(Me-mil) Extract on the anti-allergic effects. The results were obtained as follows : 1. All concentrations of Fagopyrum esculentum Moench Extract(Flours and seeds) inhibit histamine release under the vascular permeability response to intracutanenous injection. The result is proportion to concentration. But, rutin can't get considerable result. 2. All concentrations of Fagopyrum esculentum Moench Extract inhibit histamine release under the vascular permeability response to intraperitoneal injection of SD Rat in comparison with Diphenhydramine which is typical anti-histamine drug. 3. All concentrations of Fagopyrum esculentum Moench Extract inhibit histamine release under the vascular permeability response to per-oral during the three or five days. 4. In the result of quantification of histamine induced Compound $\frac{48}{80}$, Flours and seeds of Fagopyrum esculentum Moench Extract inhibit histamine release. 5. Among the fracination of Fagopyrum esculentum Moench Extract, $CHCl_3$ fraction inhibit histamine release effectively. 6. In the result of genetic manifestative inhibition about the Human mast cell treated PMA and A23187, Fagopyrum esculentum Moench Extract effect in the IL-4 and TNF-${\alpha}$ except IL-5. According to the above results, it is suggested that Fagopyrum esculentum Moench(Me-mil) Extract has anti-allergic effect.

• 대한의생명과학회지
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• 제25권1호
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• pp.54-59
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• 2019

Hepatitis refers to inflammation of hepatocytes and liver tissue, and is mainly caused by viruses, alcohol, and drugs. Forsythiae Fructus has traditionally been used as a diuretic, anti-inflammatory and antipyretic. Research on rengyolone, a bioactive substance extracted from Forsythiae Fructus, is rarely found in Korea and abroad. First, an acute animal toxicity test for rengyolone was conducted for the animal experiment. 4 week-old SD rats were injected intraperitoneally with acetaminophen for 2 weeks to induce chronic liver inflammation. Rengyolone was orally administered into two groups during 4 weeks: pre-inflammatory group and post-inflammatory group. Oral doses were also divided into 1 mg/kg and 5 mg/kg. Liver function tests (ALT, AST, ALP), western blot analysis of liver tissue, and level of inflammatory cytokine were performed to evaluate the improvement of hepatitis. Experimental results showed that rengyolone inhibited the development of acute inflammation and thus could reduce hepatitis symptoms.

• 대한의생명과학회지
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• 제28권2호
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• pp.137-144
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• 2022

Poria cocos is a natural substance known to have anticancer, antioxidant and anti-inflammatory effects. The aim of this study is to investigate the analgesic effects of Poria cocos extract (PCE). We evaluated the analgesic effects of PCE using adjuvant induced arthritis rat model. Male SD rats were administered intra-orally with PCE according to prescribed dosage, during 6 days. After 6 days later, serum TNF-α, IL-1β, and IL-6 levels were measured by ELISA. In our experiment, administration of PCE decreased TNF-α, IL-1β, IL-6 and PGE2 level in serum. Furthermore, it was confirmed that allodynia was relieved in evaluation of pain behavior. It was confirmed that administration of PCE reduces nociceptive pain by reducing nociceptive stimuli by acting as an anti-inflammatory drug.

• 생명과학회지
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• 제10권6호
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• pp.577-583
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• 2000

This study was designed to investigate the effect of silk fibroin (Mw 500)power (SFP) on oxygen radicals and their scavenger enzymes in liver membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/kg BW/day for 6 weeks. Hydroxyl radical (.OH) levels resulted in a considerable decreases (5.8% and 8.4%, 3.7% and 11.1%, respectively) in liver motochondria and micorsomes of SFP-2.5 and SFP-5.0 groups compared with control group, and $O_2$radical level was remarkably decreased about 15% and 20% in liver cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. Lipid peroxide (LPO) levels were significantly decreased (8.3% and 18.0%, 13.4% and 18.4%, respectively) in liver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were remarkably decreased about 11.6% in liver mitochondria of SFP-5.0 group compared with control group. Mn-SOD activities were remarkably in creased (17.6% and 28.8%, respectively) in mitochondria of SFP-2.5 and SFP-5.0 groups, and Cu/Zn-SOD activities were also effectively in creased (about 14.4%) in liver cytosol of SFP-5.0 groups, but significant difference between GSHPx activity in liver cytosol of these two groups could be not obtained. These results suggest that anti-aging effect of silk fibroin may play an effective anti-aging role in a aattenuating a oxidative stress and increasing a scavenger enzyme activity in liver membranes.

• 한국한의학연구원논문집
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• 제8권1호
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• pp.109-126
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• 2002

The herbal extract (YMT_02) is a modified herbal extracts from Yukmijihwangtang (YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as an anti-aging herbal medicine for hundred years in Asian countries. The purpose of this study is to; 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by hehavior task, 2) identify candidate genes responsible for enhancing memory by cDNA microarray and 3) assess the anti-oxidant effect of YMT_02 on PC12 cell. Memory retention abilities are addressed by passive avoidance task with Sprague-Dawley (SD) male rat. Before the training session, the rats are subdivided into four groups and administrated with YMT_02, Ginkgo biloba, Soya lecithin and normal saline for 10 days. The retention test was performed. 24 hours after the training session. The retention time of the YMT_02 group was significantly (p<0.05) delayed $({\sim}100%)$, whereas Ginkgo biloba and Soya lecithin treatment delayed 20% and 10% respectively. The hippocampi of YMT_02 and control group were dissected and mRNA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied and mRNA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Incyte rat GEMTM 2 cDNA microarray. The microarray results show that prealbumin(transthyretin), phosphotidy lethanolamine N-methyltransferase, and PEP-19 are expressed abundantly in the YMT_02 treated group. Especially, PEP-19 is a neuron-specific protein, which inhibits apoptotic processes in neuronal cell. On the other hand, transcripts of RAB15, glutamate receptor subunit 2 and CDK 108 are abundant in control group. Besides, neuronal genes involved in neuronal death or neurodegeneration such as neuronal-pentraxin and spectrin are abundantly expressed in control group. Additionally, the YMT_02 shows an anti oxidative effect in the PC12 cell. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the memory-enhancing effect of herbal extracts YMT_02, for example, anti-apoptotic, anti-oxidative, and neuroprotective effects.

• 핵의학기술
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• 제20권1호
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• pp.37-41
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• 2016

정도관리(Quality Control)는 오차를 최소한으로 하여 검사의 신뢰도를 증진 시키는 것을 목적으로 하고 있으며 이러한 정도관리 활동을 통해 환자의 진단과 치료방침에 영향을 주는 핵의학 혈액 검사의 신뢰도가 확보되고 있다. 이에 연구자들은 신뢰도 향상의 추가적인 기법으로 건강검진 수검자를 대상으로 시행한 핵의학 혈액검사의 모집단 분포를 이용한 통계적 품질관리의 유용성을 평가해 보고자 한다. 2014년 1월부터 2014년 12월까지 서울아산병원 건강증진센터를 이용한 건강검진 수검자 41,341명을 대상으로 핵의학 혈액 검사 12종목에 대해 통계 분석을 시행 하였다. 통계분석은 핵의학 혈액 검사 12종목에 대한 결과 보고 치를 참고치 이내, 참고치 이상, 참고치 이하의 세 가지 그룹의 월간 백분율로 구분하여 모집단 분포의 평균값과 표준편차, 그리고 표준편차 지수(Standard Deviation Index: SDI)를 산출 하였고 그 결과 모집단 분포의 표준편차는 모든 그룹에서 대부분 ${\pm}2SD$ 이내의 결과를 보였다. 건강검진 수검자에 대한 핵의학 혈액검사 12종목(AFP, CEA, CA19-9, CA125, PSA, TSH, FT4, Anti-Tg-Ab, Anti-TPO-Ab, Calcitonin, 25-OH-VitD3, Insulin)의 모집단 분포를 분석한 결과 월간 백분율의 표준편차 지수(SDI)가 ${\pm}2.0$ 이상인 경우는, 1월과 3월 Insulin검사에서 각각 2.3, 2.4, 5월 Anti-Tg-Ab검사에서 2.2, 그리고 9월 CA19-9에서 2.2의 결과를 보였다. 이는 검사 Kit 시약의 이상 (동위원소 시약의 최대 결합률 저하), 검사반응 시간의 저하가 원인 이었다. 연구대상이 되는 전체의 속성을 포함하고 모집단 분포의 특성을 이용한 통계적 품질관리는 검사실에서 시행하고 있는 내부정도관리 프로그램을 보완하는 역할을 할 수 있으리라 생각된다.