• Journal of environmental and Sanitary engineering
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• v.10 no.3
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• pp.67-77
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• 1995

This study was performed for minimization of excessive biofilm effects at the high strength organic wastewater treatment. As a results of biofilm attachment experiment using piggery wastewater, aggravation of water quality due to excessive biofilm showed after 15 days of operating times.4 excessive biofilm phase, the equivalent biofilm thickness and VSS contents per unit aura were observed in the range of 1,100 to $1,200{\mu}m$ and 2.5 to 3.0mg $VSS/cm^{2}$, respectively. In the aerobic fixed biofilm reactor/anoxic fixed biofilm reactor(AFBR/ANFBR) process with endogenous respiration phase, the BOD removal efficiency was obtained more than 90 percentage at the surface loading rate and volumetric loading rate of the AFBR maintained less than 17 g $BOD/m^{2}{\cdot}$day and 1.7kg $BOD/m^{3}{\cdot}$day, respectively. The removal efficiency of TKN and $NH_{3}$-N at the loading rates below 5.60g $NH_{3}-N/m^{2}{\cdot}day$ and 0.56kg $NH_{3}-N/m^{3}{\cdot}$day were above 76 percentage and 82 percentage, respectively. In order to reduced sludge production rate and aggravation of water quality, endogenous respiration phase was accepted at first AFBR reactor. As a results of this operating condition, sludge production was minimized and removal efficiency was maintained stability.

• Journal of Korean Society on Water Environment
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• v.22 no.6
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• pp.1014-1021
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• 2006

SMMIAR (Submerged Moving Media Intermittent Aeration Reactor) Process is a very efficient system which remove ammonia to nitrogen gas in one reactor. In this study, we determined the diversity of ammonia oxidizing bacteria and denitrifying bacteria using specific PCR amplification and the clone library construction. An ammonia monooxygenase gene(amoA) was analyzed to investigate the diversity of nitrifiers. Most of amoA gene fragments (27/29, 93%) were same types and they are very similar (>99%) to the sequences of Nitrosomonas europaea and other clones isolated from anoxic ammonia oxidizing reactors. ANAMMOX related bacteria have not determined by specific PCR amplification. A nitrite reductase gene(nirK) was analyzed to investigate the diversity of denitrifying bacteria. About half (9/20, 45%) of denitrifiers were clustered with Rhodobacter and most of others were clustered with Mesorhizobium (6/20, 30%) and Rhizobium (3/20, 15%). All of these nirK gene clones were clustered in alpha-Proteobacteria and this result is coincide with other system which also operate nitrification and denitrification in one reactor. The molecular monitoring of the population of nitrifiers and denitrifiers would be helpful for the system stabilization and scale-up.

• Journal of environmental and Sanitary engineering
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• v.17 no.3
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• pp.52-59
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• 2002

Pilot-scale PSSBR(Phase Separated Sequencing Batch Reactor) was operated to evaluate requirement of external carbon sources(${\Delta}gCOD/{\Delta}gNO_3^{-}-N$) in denitrification. Methanol and fermented food waste were used as external carbon sources. Methanol and fermented food waste were fed to the anoxic state of first reactor and concentration were 50 and 40 mgCOD/L on the basis of concentration in reactor, respectively. In case that external carbon source was not used, average $NO_3^{-}-N$ concentration in effluent was 22.49 mg/L. When methanol and fermented food waste were fed, average $NO_3^{-}-N$ concentration in effluent were 10.13 mg/L and 6.3 mg/L, respectively and requirement of external carbon sources were 4.04 and 2.5 ${\Delta}gCOD/{\Delta}gNO_3^{-}-N$, respectively. Fermented food waste was better than methanol in denitrification efficiency. Therefore fermented food waste could be one of the excellent external carbon sources for nitrogen removal in biological nutrient removal process.

• Journal of Korean Society of Water and Wastewater
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• v.13 no.1
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• pp.51-60
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• 1999

An intermittently-aerated, intermittently-decanted single-reactor process (KIDEA process : KIST intermittently decanted extended aeration process) was applied for nitrogen removal from wastewater. Synthetic wastewater with chemical oxygen demand (COD): nitrogen (N) ratio of approximately 5.25: 1 was used. The average COD removal efficiency reached above 95%, and under optimal conditions nitrogen removal efficiency also reached above 90%. This process consisted of 72 minute aeration, 48 minute settling and 24 minute effluent decanting with continuous feeding of influent wastewater from the bottom of the reactor, and did not require a separate anoxic mixing-phase. In this process, nitritation ($1^{st}$ step of nitrification) was induced but nitratation($2^{nd}$ step of nitrification) was suppressed. Main factors responsible for the accumulation of nitrite ion in the experimental condition were free ammonium and dissolved oxygen. This condition of nitrite build-up accelerated by continuous feed flow in the bottom of the KIDEA reactor because of high concentration of ammonia nitrogen in the influent. This research provides one of answers to control nitrate build-up.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.7
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• pp.479-484
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• 2013

This study was performed to examine the effect of copper on the biodegradability, nitrification, denitrification and oxygen uptake rate (OUR) using batch reactor and continuous flow stirred tank reactor (CSTR) of anaerobic/anoxic/oxic ($A_2/O$). The results of this study can be summarized as follows. In the case of the effect of copper on organic treatment, the bad effect initiated when it was above 4.5 mg/L copper with batch reactor and above 2.0 mg/L copper with CSTR. Concerning the case on nitrification and removal of nitrogen, it showed bad effect when copper was above 4.5 mg/L with batch reactor for nitrification and 1.0 mg/L with CSTR for the removal of nitrogen. The bad effect on the removal of phosphorus began when it was 4.5 mg/L copper with batch reactor and 2 mg/L copper with CSTR. In the case of OUR, it decreased as microbial activity was affected when copper concentration was above 1.5 mg/L in both case of batch reactor and CSTR.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.4
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• pp.228-233
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• 2015

This study was performed to examine the effect of zinc on the biodegradability, nitrification, denitrification and oxygen uptake rate (OUR) using batch reactor and continuous flow stirred tank reactor (CSTR) of anaerobic/anoxic/oxic ($A^2/O$). The results of this study can be summarized as follows. In the case of the effect of zinc on organic treatment, zinc had no effect up to 12 mg/L with batch reactor but biodegradability was lowered when it was above 3.0 mg/L with CSTR. Concerning the case on nitrification and removal of nitrogen, nitrification rate was lowered when zinc was above 6.0 mg/L with batch reactor and removal rate of nitrogen was lowered when zinc was above 3.0 mg/L with CSTR. Removal rate of phosphorus was lowered when it was above 6.0 mg/L zinc with batch reactor and above 3.0 mg/L zinc with CSTR. In the case of OUR, it decreased as microbial activity was affected when zinc concentration was above 3.0 mg/L in CSTR.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.2
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• pp.138-144
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• 2005

This study was carried out to investigate the changes of nitrifying bacterial populations including Nitrosomonas sp. and Nitrobacter sp. in $A^2/O$ pilot plant with the configuration of anaerobic-anoxic-oxic reactors. The suspended nitrifying bacterial populations in mixed liquor and those of attached populations on granular carrier surface made by molded waste tire were analyzed by Fluorescent in situ Hybridization(FISH) method. The nitrification rate of a pilot plant showed the value of $1.97{\sim}2.98\;mg\;N/g$ MLVSS hr. The ratios of suspended ammonia oxidizer including Nitrosomonas sp. (NSO) to total bacteria in each reactor were oxic < anoxic < anaerobic. On the contrary, the ratios of suspended nitrite oxidizer including Nitrobacter sp. (NIT) were anaerobic < anoxic < oxic. The thickness, dry density and mass of the attached biomass on granular carriers were $180{\sim}188\;{\mu}m$, $38.5{\sim}43.9\;mg/cm^3$, $29.4{\sim}32.5\;mg/g$, respectively. Also, the ratios of attached nitrifier to total bacteria on granular carriers were similar regardless of ammonia/nitrite-oxidizer (NSO; 3.2%, NIT; 2.8%) and very low compared to those(NSO; $22.8{\sim}28.4%$, NIT; $17{\sim}26%$) of suspended nitrifier.

• Journal of Korean Society on Water Environment
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• v.22 no.1
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• pp.74-82
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• 2006

In this study, development of M2-Dephanox and M3-Dephanox process has been tried to enhance the nitrogen removal of M-Dephanox process on the basis of previous study about M-Dephanox. The results showed that T-N removal efficiency of M3-Dephanox process was 8.9% or 11.3% higher than M-Dephanox or M2-Dephanox processes, respectively. This result is due to the lower $NO_3{^-}-N$ concentration in the effluent of M3-Dephanox than of M-Dephanox and M2-Dephanox processes. This results were recurrenced by PASS simulator. As result of simulation by PASS program, effluent $NO_3{^-}-N$ concentration of M3-Dephanox process was 1.4 mg/L and 3.6 mg/L lower than M-Dephanox and M2-Dephanox processes. In the study about optimization of M3-Dephanox processes by PASS program, SRT greatly affected T-N removal of M3-Dephanox process, whereas, the recycle rate and recirculation rate did little affect T-N removal efficiency of M3-Dephanox. In the study about optimization of reactors following the nitrification reactor of M3-Dephanox process, it was shown that the best optimum volume ratio of denitrification reactor, intermittently aerated reactor and anoxic reactor for the T-N removal were 29.1(%) : 32.7(%) : 38.2(%). T-N removal efficiency at this volume ratio was similar to T-N removal efficiency at the volume ratio of 36.3(%) : 36.3(%) : 27.4(%) designed for the lab-scale M3-Dephanox.

• Environmental Engineering Research
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• v.14 no.3
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• pp.174-179
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• 2009

The InSub system(applied for a patent) was developed, as it combined the indirectly aerated submerged biofiltration(InSub) reactor and Anaerobic/ Anoxic reactor. This system which can eliminate organism and nutrient materials at the same time, which is safe and economical to be maintained and managed is more simple process than the complicated existing biological advanced sewage treatment system. The most suitable HRT of this study showed 9 hours. As looking into the effluent concentration and removal efficiency of each item at 9 hours of HRT, each effluent concentration for $SS,\;BOD5,\;COD_{Mn},\;and\;COD_{Cr}$ was 1.46 mg/L, 7,09 mg/L, 9.84 mg/L and 16.42 mg/L. And their removal efficiency was 96.98%. 90.59%, 77.18% and 83.92%, respectively. Each effluent concentration of T-N and T-P was 10.42 mg/L and 1.04 mg/L. Their removal efficiency was 73.38% and 61.62%, respectively. This pilot plant experiment(the state was without the internal recycling.) followed a variety of HRT. The results confirmed that it was to be advanced sewage treatment system with high efficiency when it combined with the internal recycling.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.5
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• pp.622-629
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• 2014

This study induced biological denitrification and nitrification via a biofiltration process with the view of removing nitrogen from land-based fish farm effluent. To achieve this, we operated an aquaculture nitrogen-removal system that includes a denitrification and nitrification reactor [working volume 40 L, flow rate 64.8 L, HRT (hydraulic retention time) 14.8 h, HRT considering recycling of NOx 7.4 h]. In the continuous process, the nitrification rate of ammonium nitrogen exceeded 90% at a steady state and the denitrification efficiency exceeded 80% with recycling to a pre-anoxic reactor. In addition, the pH in the final effluent was lower with a low influent water alkalinity averaging 100 mg/L (as $CaCO_3$). For effective denitrification reactions, carbon must be supplied via particulate organic matter (POM) hydrolysis because of the low C/N (carbon/nitrogen) ratio in the water.