• 제목/요약/키워드: Animal-being

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바이오가스 이용 기술지침 마련을 위한 연구(I) - 현장조사 결과 중심으로 (A Study on Establishment of Technical Guideline of the Installation and Operation for the Biogas Utilization of Power Generation and Stream - Results of the Field Investigation)

  • 문희성;배지수;박호연;전태완;이영기;이동진
    • 유기물자원화
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    • 제26권1호
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    • pp.55-64
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    • 2018
  • 본 연구는 유기성폐자원(가축분뇨, 음식물류폐기물, 음식물류폐수 등)의 바이오가스 이용에 대한 적정 설계 및 운전 기술지침서 마련하고자 현장조사와 정밀모니터링 등을 실시하였다. 정부의 중장기 바이오가스화 정책에 따라 폐자원의 자원화 시설 확충이 활발히 추진되고 있다. 하지만 생산된 바이오가스를 이용하여 발전 및 스팀으로 활용하는 시설은 효율이 아직은 저조하고 잦은 고장이 발생되고 있다. 현재 운영 중인 바이오가스화 11개소를 대상으로 바이오가스 이용 공정별 특징 및 문제점을 분석하고 혐기소화조 현장 시료의 계절별 정밀모니터링을 실시하여 바이오가스 이용 공정 흐름에 따른 설계 운영 가이드라인을 제시하였다. 현장조사 수행 결과, 바이오가스 이용 공정에서 바이오가스의 적정 제습, 황화수소 전처리, 발전시설의 적정처리 등이 주요 문제점으로 조사되었다. 따라서 유기성폐자원의 바이오가스화 시설의 단점을 보완하고 바이오가스 이용 최적화 방안을 마련하기 위하여 현장지설에 대한 정밀조사를 실시하여 공정별 문제점들을 정리하였다.

고려 홍삼이 수면의 질에 미치는 영향 : 무작위 위약-대조군 연구 (Effect of Korean Red Ginseng on Sleep : A Randomized, Placebo-Controlled Trial)

  • 이선아;강승걸;이헌정;정기영;김린
    • 수면정신생리
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    • 제17권2호
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    • pp.85-90
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    • 2010
  • 목 적: 인삼은 한국을 비롯한 아시아 국가에서 전통적으로 불면증의 치료에 사용되어 왔으며 쥐를 이용한 동물실험에서 수면을 촉진시키는 효과가 있다는 연구 결과가 있어왔다. 하지만 이러한 효과를 입증할 만한 임상 실험은 거의 이루어지지 않았다. 이에 본 연구에서는 고려 홍삼이 수면에 미치는 영향을 알아보고자 한다. 방 법: 건강한 성인 남성 20명을 대상으로 하였으며 이들은 무작위로 홍삼군과 위약군에 배정되어 2주간의 실험이 진행되었다. 홍삼군의 경우 하루 4,500 mg의 홍삼을 섭취하였다. 실험 시작시점과 2주 후인 종료시점에 수면다원검사를 시행하여 두군간의 변수의 변화를 비교하였다. 결 과: 총 15명의 대상자, 즉 홍삼군 8명과 위약군 7명이 수면다원검사를 시행 받았으며 홍삼군이 위약군 보다 실험 종료 시점에서 3단계 수면이 증가한(p=0.087) 반면에 2단계 수면이 감소하는(p=0.071) 경향을 나타냈다. 결 론: 본 연구 결과는 고려 홍삼이 깊은 잠은 증가시키고 얕은 잠은 감소시키는 경향이 있음을 보여준다. 이는 통계적으로 유의한 결과는 아니지만 홍삼이 수면의 질을 향상시키는 데 효과적임을 짐작하게 한다. 그러나, 보다 많은 대상군과 충분한 홍삼 용량으로 보다 긴 기간 동안 진행되는 연구를 통해 고려 홍삼의 수면에 대한 효과를 확인할 필요가 있다.

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Comparative genetic diversity of wild and released populations of Pacific abalone Haliotis discus discus in Jeju, Korea, based on cross-species microsatellite markers including two novel loci

  • An, Hye-Suck;Hong, Seong-Wan;Kim, En-Mi;Lee, Jeong-Ho;Noh, Jae-Koo;Kim, Hyun-Chul;Park, Chul-Ji;Min, Byung-Hwa;Myeong, Jeong-In
    • Animal cells and systems
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    • 제14권4호
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    • pp.305-313
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    • 2010
  • Pacific abalone Haliotis discus discus is an important fisheries resource in Jeju, Korea. For basic information about its current genetic status in relation to stock enhancement, the level and distribution of genetic variation between wild and released stocks of Pacific abalone in Jeju were examined at nine cross-species microsatellite markers including the use of two novel primers. High levels of polymorphism were observed between the two populations. A total of 146 different alleles were found at all loci, with some alleles being unique. The allelic variability ranged from five to 27 in the wild population and from four to 16 in the released sample. The average observed and expected heterozygosities were estimated to be 0.74 and 0.84 in the wild sample and 0.70 and 0.78 in the released sample, respectively. Although a considerable loss of rare alleles was observed in the released sample, no statistically significant reductions were found in heterozygosity or allelic diversity in the released sample compared to the wild population. Low but significant genetic differentiation was found between the wild and released populations. These results suggest that the intensive breeding practices for stock enhancement may have resulted in a further decrease in genetic diversity, and that the cross-species microsatellite markers used in this study represent a potentially efficient means for further genetic studies, providing beneficial information for the protection and management of H. discus discus.

카빙 데코레이션 인지도와 구매 의도가 가격 수용성에 미치는 영향 - 대구 지역을 중심으로 - (The Effect of the Recognition of Carving Decoration Purchase Intention on Acceptable Price Range - Focused on Daegu Area -)

  • 김기진;변광인
    • 한국조리학회지
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    • 제16권4호
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    • pp.28-42
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    • 2010
  • 본 연구는 식공간 연출, 푸드 스타일링, 테이블 데코레이션의 한 부분이라 할 수 있는 카빙 데코레이션에 대해 소비자들의 인식과 구매 의도 및 가격 수용성에 대해 살펴보고자 하였다. 본 연구는 2009년 5월 18일부터 2009년 5월 22일까지 대구 지역에 아침 시간과 저녁 시간에 버스와 지하철을 이용하는 20세 이상의 성인을 대상으로 실시하였고, 296부를 최종 분석에 사용하였다. 연구결과 50% 이상의 소비자들이 카빙에 대해 알고 있었고, 대부분 TV를 통해서 알게 되었다고 한다. 카빙은 뷔페와 레스토랑에 가장 많이 사용될 것이라고 하였고, 뷔페와 일식에서 가장 많이 사용될 것이라 하였다. 또한, 꽃-나비 카빙과 일식이, 동물 형태 카빙과 중식이, 수박 카빙과 뷔페가 가장 잘 어울린다고 하였다. 아울러 카빙 데코레이션에 대해 인지도가 높은 소비자가 구매 의도와 가격 수용성이 높은 것으로 나타났고, 카빙 데코레이션을 인지도가 낮은 소비자는 구매 의도가 높다고 해도 가격 수용성이 매우 낮게 지각하지만, 카빙 데코레이션 인지도가 높은 소비자의 구매 의도가 높은 경우에는 가격 수용성이 매우 높게 나타났다.

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우슬산(牛膝散)의 항혈전작용(抗血栓作用)에 대(對)한 실험적(實驗的) 연구(硏究) (The Experimental Study on Antithrombotic activities of Wuslsan)

  • 김경수;신용완;김의일;김수민;이정은;유동열
    • 대한한방부인과학회지
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    • 제18권3호
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    • pp.110-126
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    • 2005
  • Purpose : The Purpose of this research was to investigate the effects of antithrombotic activities of Wuslsan (WSS). Methods : Measure the effect which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD soulution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. Being classified one group of eight mice, each of them was divided into Normal, Control, and WSS. The normal group supplied a saline solution and the control group brought the dextran extravasated blood after an hour of administering the saline solution. Also WSS was dissolved in 2ml saline solution and then we dosed it to the experimental mice with Oral Zonde one day before the experiment. After that, the mice were abstained from food. And then we gave a measured amount of it before an hour. Finally, it gave rise to dextran extravasated blood in the same way as the Control group. Results : The results were obtained as follows. WSS inhibited platelet aggregation induced by ADP and epinephrine significantly as compared with the control group. WSS showed fibrinolytic activity insignificantly as compared with the control group. WSS increased blood flow rate significantly as compared with the control group in vitro. WSS inhibited pulmonary embolism induced by collagen and Epinephrine(inhibitive rate is 37.5%). WSS increased number of platelet and fibrinogen amount significantly, and shortened PT and APTT as compared with the control group in thrombus model induced by dextran. Conclusion : WSS is effective antithrombotic activity from experimental result.

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A Simple Method for the Preparation of Crude Gintonin from Ginseng Root, Stem, and Leaf

  • Pyo, Mi-Kyung;Choi, Sun-Hye;Shin, Tae-Joon;Hwang, Sung-Hee;Lee, Byung-Hwan;Kang, Ji-Yeon;Kim, Hyeon-Joong;Lee, Soo-Han;Nah, Seung-Yeol
    • Journal of Ginseng Research
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    • 제35권2호
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    • pp.209-218
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    • 2011
  • Ginseng has been used as a general tonic agent to invigorate the human body as an adaptogenic agent. In a previous report, we have shown that ginseng contains a novel glycolipoprotein called gintonin. The main function of gintonin is to transiently enhance intracellular free $Ca^{2+}$ $[Ca^{2+}]_i$ levels in animal cells. The previous method for gintonin isolation included multiple steps using organic solvents. In the present report, we developed a simple method for the preparation of crude gintonin from ginseng root as well as stem and leaf, which produced a higher yield of gintonin than the previous one. The yield of gintonin was 0.20%, 0.29%, and 0.81% from ginseng root, stem, and leaf, respectively. The apparent molecular weight of gintonin isolated from stem and leaf through sodium dodecyl sulfate polyacrylamide gel electrophoresis was almost same as that from root but the compositions of amino acids, carbohydrates or lipids differed slightly between them. We also examined the effects of crude gintonin from ginseng root, stem, and leaf on endogenous $Ca^{2+}$-activated $Cl^-$ channel (CaCC) activity of Xenopus oocytes through mobilization of $[Ca^{2+}]_i$. We found that the order of potency for the activation of CaCC was ginseng root > stem > leaf. The $ED_{50}$ was $1.4{\pm}1.4$, $4.5{\pm}5.9$, and $3.9{\pm}1.1$ mg/mL for root, stem and leaf, respectively. In the present study, we demonstrated for the first time that in addition to ginseng root, ginseng stem and leaf also contain gintonin. Gintonin can be prepared from a simple method with higher yield of gintonin from ginseng root, stem, and leaf. Finally, these results demonstrate the possibility that ginseng stem and leaf could also be utilized for ginstonin preparation after a simple procedure, rather than being discarded.

Changes of Ginsenoside Content by Mushroom Mycelial Fermentation in Red Ginseng Extract

  • Bae, Song-Hwan;Lee, Hyun-Sun;Kim, Mi-Ryung;Kim, Sun-Young;Kim, Jin-Man;Suh, Hyung-Joo
    • Journal of Ginseng Research
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    • 제35권2호
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    • pp.235-242
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    • 2011
  • To obtain microorganisms for the microbial conversion of ginsenosides in red ginseng extract (RGE), mushroom mycelia were used for the fermentation of RGE. After fermentation, total sugar contents and polyohenol contents of the RGEs fermented with various mushrooms were not a significant increase between RGE and the ferments. But uronic acid content was relatively higher in the fermented RGEs cultured with Lentus edodes (2155.6 ${\mu}g/mL$), Phelllinus linteus (1690.9 ${\mu}g/mL$) and Inonotus obliquus 26137 and 26147 (1549.5 and 1670.7 ${\mu}g/mL$) compared to the RGE (1307.1 ${\mu}g/mL$). The RGEs fermented by Ph. linteus, Cordyceps militaris, and Grifola frondosa showed particularly high levels of total ginsenosides (20018.1, 17501.6, and 16267.0 ${\mu}g/mL$, respectively). The ferments with C. militaris (6974.2 ${\mu}g/mL$), Ph. linteus (9109.2 ${\mu}g/mL$), and G. frondosa (7023.0 ${\mu}g/mL$) also showed high levels of metabolites (sum of compound K, $Rh_1$, $Rg_5$, $Rk_1$, $Rg_3$, and $Rg_2$) compared to RGE (3615.9 ${\mu}g/mL$). Among four different RGE concentrations examined, a 20 brix concentration of RGE was favorable for the fermentation of Ph. linteus. Maximum biotransformation of ginsneoside metabolites (9395.5 ${\mu}g/mL$) was obtained after 5 days fermentation with Ph. linteus. Maximum mycelial growth of 2.6 mg/mL was achieved at 9 days, in which growth was not significantly different during 5 to 9 days fermentation. During fermentation of RGE by Ph. linteus in a 7 L fermenter, $Rg_3$, $Rg_5$, and $Rk_1$ contents showed maximum concentrations after 5 days similar to flask fermentation. These results confirm that fermentation with Ph. linteus is very useful for preparing minor ginsenoside metabolites while being safe for foods.

경관훼손지의 생태적 복구방안에 관한 연구 (A Study on the Ecological Restoration Strategies for the Disturbed Landscapes)

  • 김남춘
    • 한국환경복원기술학회지
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    • 제1권1호
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    • pp.28-44
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    • 1998
  • This study was conducted to suggest the ecological restoration strategies for the disturbed landscapes by theoretical study. Especially, it is aimed to suggest three objectives for restoration by using native plants ; (1) prevention or reduction of wind and water erosion, (2) provision of food and cover for variety of animal species, (3) improvement of the visual or aesthetic quality of disturbed sites. The main results were summarized as follows. 1. Cooperation between restoration ecologists and restoration practitioners is needed to delineate a scientific approach to restoration ; (1) Being aware of published literature that describes similar work and/or establish general principles. (2) Preparedness to carry out proper experiments to test ideas. (3) Preparedness to monitor fundamental parameters in a restoration scheme. (4) Providing information about the behavior of species. (5) Publish results. 2. There are three models of succession in theories of plant succession. The tolerance and facilitation models were recommended to ecological restoration. The inhibition model applies in most secondary succession, but the actual species which are introduced first may inhibit the germination and growth of slower-growing species, or they may prevent the growth of other species whose propagules arrive later. 3. The objectives of erosion control, wildlife habitat provision, and visual quality improvement are not mutually exclusive. However, many revegetation practices in the past have emphasized one of these aspects at the expense of the others. 4. A native plant community can be the model of ecological restoration. By stylization/abstraction of native plant community, trying to learn the most essential characteristics of community types - environmental factors ; dominant, prevalent, and "visual essence" species composition - in order to use such information in restoration. 5. After developing mass/spaces plan, match plant communities to the mass/space plan. In utilizing community grouping, there needs aesthetic ability to understand design elements. 6. Several hydrophytes such as Pennisetum saccharifluous, Themeda triandra, Cirsium pendulum show relatively good germination rates. In case of mesophytes and xerophytes, Arundinella hina, Artemisia princeps, Oenothera odorata and legumes seem to have quick-germinating abilities at barren sites. Pinus thunbergii, Rhus chinensis, Evodia daniellii, Alnus firma and Albizzia julibrissin can be considered as "late succession" woody plants because they show low germinating rates and slow growing habitat. 7. The seeds used for restoration should be collected within a certain radius of where it will be planted. Consideration in genetic issues in the collection and use of germplasm can increase the odds for successful restoration efforts. 8. The useful model in the "drift" pattern occurs so abundantly in naturally evolving landscapes. As one species diminishes in density, a second or third species are increased. Thus, dynamic interactions between species are created. Careful using of "drift phenomenon" in planting was recommended. 9. Virtually no stand of vegetation today is immune from the introduction and/or spreading of exotic species. Therefore, the perpetuation of a restored sites requires conscious monitoring and management. Thus, management would be the most important process in ecological restoration. 10. In order to keep the sites "natural", alternative management strategies would be applied in management ; Atenative management strategies are decelerating successional process, accelerating successional processes or modifying species composition. As management tools, mechanical, chemical, biological or environmental factor manipulation, e.g., fire could be used.

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돼지 단위생식란의 세포사멸 유전자 발현 양상에 관한 연구 (Expression Analysis of Programmed Cell Death Genes in Porcine Parthenogenesis)

  • 손종윤;김상환;정덕원;류춘열;윤종택
    • 한국수정란이식학회지
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    • 제30권3호
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    • pp.239-248
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    • 2015
  • The nature of molecular mechanisms governing embryonic cell block is largely unknown, but recent reports have demonstrated that proper execution of programmed cell death is crucial for this process. The main objective of this study is to determine effects of programmed cell death on porcine oocytes development in vitro after parthenogenesis. Among the blastocysts matured in 3MA, MAP1LC3A and ATG5 RNA gene expression level increased in the order of Cyst < 3MA < RP. However, Casp-3 and TNF-r RNA gene expression level decreased in the order of RP < 3MA < Cyst. Expression of mTOR within the RP-cultured blastocyst was the most highly to the inner cell mass, while 3MA-cultured blastocyst showed very lowest expression in inner cell mass. The expression of mTOR showed a pattern opposite to that of MAP1LC3A. That is, its expression was the lowest in Cyst group. When the enzymatic activity of MMP-2 and MMP-9 was assessed in culture, the level of active MMP-9 was higher expression in the medium of each RP treatment group, with the level of another treatment group being relatively higher. Analyses of TIMP-2 and TIMP-3 revealed that their expression was higher in groups that did not receive RP treatment. More specifically, the level of TIMP-2 was not affected by Cyst treatment, while the level of TIMP-3 was higher in 3MA and RP treatment group. There was highly cell division activation efficiency of parthenogenesis on cultured system of RP supplement IVC medium. Therefore, these results suggest that embryo development was significantly increased in conditional culture medium with active autophagy as compared to common cultured condition. Further investigation of this distinction may enable the development of innovative improvements for the production of porcine somatic cell nuclear transfer.

한·미 FTA가 한라봉 시장에 미치는 경제적 파급영향 분석 (An Analysis of Economic Impacts of Korea-US FTA on Hallabong Market)

  • 김태련;김화년;김배성
    • 한국산학기술학회논문지
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    • 제21권1호
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    • pp.725-731
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    • 2020
  • 이 연구는 한·미 FTA 발효 이후 오렌지가 수입되어 집중적으로 판매되는 시기인 1월부터 5월까지 초점을 맞춰 오렌지 수입량 증가가 국내산 과일에 미치는 영향을 분석하고자 하였다. 본 연구에서는 미국산 오렌지와 경합 되는 국내산 과일로는 감귤류만으로 한정하였고, 그중 미국산 오렌지와 소비 시기가 일치하는 한라봉을 분석대상으로 선정하였다. 이를 위해 동태 축차적 시뮬레이션 모형을 구축하여 한·미 FTA 사후 영향평가 및 한라봉 시장의 중장기 전망을 실시하였다. 또한, 한라봉 시장에 미치는 주요 정책 시뮬레이션을 수행하여 각 시나리오별 파급영향을 계측하였다. 사후 영향평가결과, 한라봉은 주 출하기인 12월부터 2월 사이에는 오렌지 계절관세의 영향은 없으나 TRQ 물량의 영향으로 2012년~2017년 실질 조수입이 연평균 21.9억 원 감소한 것으로 나타났고, 6년 감소 누계액은 131.5억 원 수준인 것으로 나타났다. 주요 정책 시뮬레이션 분석결과, 미국의 오렌지 작황에 따른 수입단가 변화 및 한라봉 수출량 증대는 한라봉 시장의 규모를 증대시키고 이에 따라 소득증대 효과를 가져오는 것으로 나타났다.