• Asian-Australasian Journal of Animal Sciences
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• 제2권2호
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• pp.77-84
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• 1989

Twenty-four Hereford steers, 22 months old and a mean liveweight (${\pm}\;s.e.$) of $250\;{\pm}\;7\;kg$ were used in an experiment to evaluate over 42 days two rates of copra meal supplementation to cattle on a low N ($8.6\;{\pm}\;0.9$ g N/kg dry matter (DM)), low digestible ($45\;{\pm}\;5.2%$ DM) native pasture hay. Steers given the two rates (500, 1000 g/steer/day; i.e. 500C, 1000C) were compared to steers on a non-supplemental diet and to the effects on steers of supplemental urea (30g/steer/day; 30U) or with copra meal (500 g/steer/day; 500C.U), or of cottonseed meal (500 g/steer/day; 500S). Liveweight change was increased (P<0.01) by all of the supplements except by supplemental urea. The most effective treatment, 1000C, increased significantly (P<0.01) liveweight change (946 g/day) in steers above all supplements except those steers given 500C.U (718 g/day). Hay intake per unit liveweight was increased (P<0.05) by 7% by the 30U and 500C.U treatment, and by 9% by 500C; this group having the highest supplements, being greatest (P<0.05) for the 1000C group (6.0 g feed intake/g gain) and least for the 500S supplemented group (11.5 g/g gain). Efficiency was lowest (18.6 g/g gain) for the non-supplemented steers on the basal hay diet. Copra meal N was less degradable (i.e. 29%) in nylon bags over 15 hours in the rumen than was cottonseed meal N (37%), and rumen ammonia concentrations were lower (P<0.05) in cattle supplemented with copra meal (25, 27 mg N/L) than in cattle given urea (36 mg N/L) or cottonseed meal (39 mg N/L). It is concluded that copra meal at a daily rate of 500 g/head, and with rumen soluble nitrogen from urea, is an effective supplement for improving growth of cattle on a low quality forage.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2405-2425
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• 2014

Radiation exposure leads to several pathophysiological conditions, including oxidative damage, inflammation and fibrosis, thereby affecting the survival of organisms. This review explores the radiation countermeasure properties of fourteen (14) plant extracts or plant-derived compounds against these cellular manifestations. It was aimed at evaluating the possible role of plants or its constituents in radiation countermeasure strategy. All the 14 plant extracts or compounds derived from it and considered in this review have shown some radioprotection in different in vivo, ex-vivo and or in vitro models of radiological injury. However, few have demonstrated advantages over the others. C. majus possessing antioxidant, anti-inflammatory and immunomodulatory effects appears to be promising in radioprotection. Its crude extracts as well as various alkaloids and flavonoids derived from it, have shown to enhance survival rate in irradiated mice. Similarly, curcumin with its antioxidant and the ability to ameliorate late effect of radiation exposure, combined with improvement in survival in experimental animal following irradiation, makes it another probable candidate against radiological injury. Furthermore, the extracts of P. hexandrum and P. kurroa in combine treatment regime, M. piperita, E. officinalis, A. sinensis, nutmeg, genistein and ginsan warrants further studies on their radioprotective potentials. However, one that has received a lot of attention is the dietary flaxseed. The scavenging ability against radiation-induced free radicals, prevention of radiation-induced lipid peroxidation, reduction in radiation cachexia, level of inflammatory cytokines and fibrosis, are some of the remarkable characteristics of flaxseed in animal models of radiation injury. While countering the harmful effects of radiation exposure, it has shown its ability to enhance survival rate in experimental animals. Further, flaxseed has been tested and found to be equally effective when administered before or after irradiation, and against low doses (${\leq}5Gy$) to the whole body or high doses (12-13.5 Gy) to the whole thorax. This is particularly relevant since apart from the possibility of using it in pre-conditioning regime in radiotherapy, it could also be used during nuclear plant leakage/accidents and radiological terrorism, which are not pre-determined scenarios. However, considering the infancy of the field of plant-based radioprotectors, all the above-mentioned plant extracts/plant-derived compounds deserves further stringent study in different models of radiation injury.

• 한국축산시설환경학회지
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• 제8권3호
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• pp.153-158
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• 2002

활성오니처리를 한 양돈폐수 방류수의 잔여오염물질을 정화하고 색도 구성물질을 분해하고자 4개의 오존처리조와 폭기조, 침전조 등으로 구성된 처리소 총 처리조용량이 594.5l이고 1일 200l씩 처리가 가능한 20g/hr의 오존발생기가 부착된 오존이용 탈색 시스템을 개발하였으며 그 시험 결과는 다음과 같다. 1. 오존처리시 온도는 반응열에 의하여 처리전 31.$0^{\circ}C$에서 처리중에는 41.9$^{\circ}C$까지 증가하였으며 pH도 처리 과정 중에 계속적으로 증가했다. 2. 산화환원전위차는 처리전에 148㎷였으나 처리중에 증가하여 330㎷까지 증가하였으며 처리가 완료된 후 방류조에서는 다시 감소하였다. 3. BOD의 경우 처리전 11mg/l였으나 처리후에 1mg/l로 낮아졌으며 CO $D_{MN}$ 의 경우에도 83mg/l에서 1.0mg/l으로 낮아졌다. 4. 활성오니처리 방류수의 색도는 442도였으나 오존처리후에는 색도가 6도로 98.6%의 높은 색도제거효율을 보였다.

• 한국축산식품학회지
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• 제35권3호
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• pp.370-381
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• 2015

This study was designed to determine the usability of lemon fiber (LF-2%, 4%, 6%) and carrot fiber (CF-2%, 4%, 6%) to produce lowfat beef hamburgers. To that end, a certain amount of fat was replaced with each fiber. The proximate composition, pH value, cholesterol content, cooking characteristics, color, texture profile, and sensory properties of low-fat beef hamburgers were investigated. LF increased moisture content and cooking yield due to its better water binding properties, while CF caused higher fat and cholesterol contents owing to its higher fat absorption capacity (p<0.05). LF resulted in a lighter, redder, and more yellow color (p<0.05). Hardness, gumminess, springiness, and chewiness parameters decreased when the usage level of both fibers increased (p<0.05). However, more tender, gummy, springy, and smoother hamburgers were produced by the addition of CF in comparison with LF (p<0.05). Moreover, hamburgers including CF were rated with higher sensory scores (p<0.05). In conclusion, LF demonstrated better technological results in terms of cooking yield, shrinkage, moisture retention, and fat retention. However it is suggested that CF produces better low-fat hamburgers since up to 2% CF presented sensory and textural properties similar to those of regular hamburgers.

• Asian-Australasian Journal of Animal Sciences
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• 제13권5호
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• pp.636-640
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• 2000

Cup-plant (Silphium perfoliatum L.) has potential to produce high biomass and highly digestible forage in the wetlands where other productive forages do not grow or produce well. However, high moisture content at harvest is a considerable disadvantage of cup-plant for the production of high quality silage. This study was conducted to determine the effect of moisture content on the characteristics of cup-plant silage. Harvested cup-plant was ensiled in farm scale plastic bag silos and laboratory silos. In the plastic bag silos, first growth (FG) and regrowth (RG) cup-plant was harvested, wilted and ensiled. Dry matter content of FG and RG was 280 g/kg and 320 g/kg after 48 hr of wilting. The silage made with FG had pH 5.3 and 5.63 g/kg DM of acetate as a major volatile fatty acid. The composition of lactate, butyrate and acetate production was 1.0: 0.9: 2.3. The pH of silage made with RG was 4.5 and lactate was a major fermentation end product (16.8 g/kg DM). In the laboratory silos, wilted and unwilted first growth cup-plant material was ensiled to compare the early fermentation end products at days 2, 4, 11, and 40. Wilting increased dry matter content by 42% in the harvested material. Wilted silage showed about one unit lower pH until day 11. The contents of ammonia nitrogen and acetate were higher in un wilted silage, while that of lactate was higher in wilted silage (p<0.05). Butyrate and propionate were not detected in the wilted silage until day 40. We conclude from the results that moisture control is essential for the production of high quality cup-plant silage and high pH of cup-plant silage is due to low concentrations of fermentation end products.

• Asian-Australasian Journal of Animal Sciences
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• 제16권8호
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• pp.1218-1223
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• 2003

The effects of type (high and low voltages) and time (3, 40 and 60 min postmortem) of stimulation on drip loss and meat color at 24 h post-mortem were determined on M. longissimus dorsi of 38 crossbred steers and heifers. In addition, the effect of pH early postmortem (70 min postmortem) on the rate and extend of meat tenderization was examined. Either high or low voltage stimulation at 3 min showed a tendency for faster pH decline (p=0.052) and higher drip loss (p=0.08), and improved the color dimensions of L*, a* and b* (p<0.01), compared to stimulation at 40 min. This was equivalent to approximately one unit of an AUSMEAT color chip. On the other hand, although there were significant differences in pH decline between high voltage stimulation at 40 and 60 min, and between low voltage stimulation at 40 min and control sides, drip loss and meat color did not differ significantly (p>0.05). The results suggested that early application of stimulation, regardless of type of stimulation, improved overall meat color at 24 h postmortem through its effect on faster glycolysing rate. However, if the pH decline was moderate, the benefit of electrical stimulation on meat color was not apparent. An intermediate pH decline resulted in the lowest shear force. Due to differential ageing rates the optimum pH at 70 min postmortem increased with ageing time from 5.96, 6.07, 6.12 and 6.14 for 1, 3, 7 and 14 days postmortem, respectively. This implied that a small difference in the rate of pH decline was important, especially carcasses stimulated for very early postmortem, and the optimum rate of pH decline varied with intended ageing period. The study suggests that the beneficial or adverse effects of electrical stimulation on drip loss, meat color and tenderness is determined by the rate of pH decline, rather than by stimulation treatment and time of application per se.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.606-612
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• 2005

Individual production traits, such as reproduction and mortality rates, are partial measures, but may be used to evaluate the performance of different systems by comparing the rate of flock growth and potential offtake. The productivity of two existing sheep production systems, one extensive, one intensive, was compared with an alternative semi-intensive system. The future flock sizes, offtakes and structures were predicted based on the age structure of the flock and age-specific reproduction, mortality and growth rates. The measurements were illustrated with reference to growth of a sheep flock of different age and sex categories. The flock was in a socalled dynamic situation. During the dry period, the digestible organic matter intake of the animals in the intensive system and both extensive and semi-extensive systems was 36 and 20.1 g kg$^{-0.75}$ d$^{-1}$, respectively. During the cold period, the digestible organic matter intake of the animals in extensive, intensive and semi-extensive systems was 34, 34.5 and 41 g kg$^{-0.75}$ d$^{-1}$, respectively. During the dry period, the animals in the both extensive and semi-intensive systems lost in body weight at a rate of 19 g per day, but the rate of gain in body weight of the animals in intensive system was 57 g per day. During the cold period, the animals in extensive, intensive and semiintensive systems gained in body weight at rates of 56, 67 and 97 g per day, respectively. The higher gain of animals during the cold period in the semi-intensive system was related to a sustained higher intake of low-quality roughage and more efficient use of the available feed. Compared to the intensive system, the annual concentrate input of the semi-intensive system was about 48% lower for each livestock unit. The productivity of the semi-intensive system was higher than that of the extensive system.

• Asian-Australasian Journal of Animal Sciences
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• 제9권5호
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• pp.577-582
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• 1996

In order to investigate the relationship between daily herbage allowance (DHA) and daily herbage intake (DHI) on the points of leaf and stem masses, grazing experiments were carried out on the pasture of tall fescue (Festuca arundinacea Schreb.). The experiments consisted of four 3-days' grazing periods which were from August 14 to 17, August 17 to 20, October 12 to 15 and October 15 to 18 in 1990. The value of DHA was 32.1 to 84.3, and the value of DHI was 9.9 to 27.0. The index of leafiness (leaf to stem ratio, LSR) was 0.74 to 3.06. The relationship between DHI and DHA was quadratic. Both relationship of daily leaf intake (DLI) to daily leaf allowance (DLA) and that of daily stem intake (DSI) to daily stem allowance (DSA) were likely to be asymptotic forms, although DHI did not show any significant relationships to DLA nor to DSA. The relationship between DLI and LSR was quadratic, and a negative relationship was found between DSI and LSR. From the relationship of DLI to LSR and that of DSI to LSR, the relationship of daily intake of leaf and stem (DLSI) to LSR was derived as quadratic and the LSR value at which the maximum DLSI was attained was estimated. The DHA, DHI, DLA, DLI, DSA, DSI and DLAI were expressed as the same unit of g/kg live body weight/day.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1363-1370
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• 2016

Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production. Yaks total RNA was extracted from the abomasum of an unweaned yak. The yak preprochymosin, prochymosin, and chymosin genes from total RNA were isolated using gene specific primers based on cattle chymosin gene sequence respectively and analyzed their expression pattern byreal time-polymerase chain reaction. The result showed that the chymosin gene expression level of the sucking yaks was 11.45 times higher than one of adult yaks and yak chymosin belongs to Bovidae family in phylogenetic analysis. To express each, the preprochymosin, prochymosin, and chymosin genes were ligated into the expression vector $pPICZ{\alpha}A$, respectively, and were expressed in Pichia pastoris X33. The results showed that all the recombinant clones of P. pastoris containing the preprochymosin, prochymosin or chymosin genes could produce the active form of recombinant chymosin into the culture supernatant. Heterologous expressed prochymosin (14.55 Soxhlet unit/mL) had the highest enzyme activity of the three expressed chymosin enzymes. Therefore, we suggest that the yak chymosin gene recombinant Pichia pastoris strain could provide an alternative source of rennet production.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.511-516
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• 2007

This experiment was conducted to evaluate the effects of differently maturing corn hybrids on silage production and milk production per unit area in the northern part of Japan, where grain development occurs under decreasing ambient temperature. Both hybrids were harvested at the same time. The stages of maturity for the early-maturing hybrids (EH; 80 d relative maturity) and the mid-maturing hybrids (MH; 93 d relative day) were early dent and late dough stage, respectively. The plant yields for MH were higher than those for EH. The dry matter (DM) content of MH was lower than that for EH, and the effluent loss for MH silage was greater than that for EH silage. Therefore, the DM yields of prepared silage per area were similar for both treatments. Twelve multiparous mid-lactation Holstein cows ($58{\pm}13$ days in milk) were fed diets based on EH or MH silage in a crossover design with two 3-week periods. Cows were fed 3 kg of hay crop silage (DM basis) and either EH or MH silage ad libitum, and concentrates were supplied to meet NRC requirement for dairy cows. Silage DM intake for EH was found to be higher (p<0.05) than that for MH (10.0 vs. 9.1 kg/day). Milk production and milk composition for EH were similar to those for MH. Feed efficiency per total feed intake was similar in both treatments, although the feed efficiency per concentrate intake tended to be higher for the EH than that for the MH diet. These results indicate that differences in maturation in corn hybrids affect the effluent production of silage and the silage intake of dairy cows. It may be advantageous to plant early hybrid corn with a reduction in effluent production of silage as well as a reduction in purchased feed costs for dairy cows under the climatic conditions of the northern part of Japan.