• Journal of agriculture & life science
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• v.50 no.3
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• pp.129-139
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• 2016

Curcumin plays a protective role in brain injury through its anti-oxidant and anti-inflammatory activities. Moreover, peroxiredoxin-5 exerts a protective effect against oxidative stress. The aim of this study was to investigate whether curcumin modulated the peroxiredoxin-5 expression in focal cerebral ischemic animal model. Middle cerebral artery occlusion(MCAO) was performed to induce cerebral ischemic injury in rats. Adult male rats were injected intraperitoneally with vehicle or curcumin(50mg/kg B.W.) 1 h after MCAO and cerebral cortex tissues were collected 24 h after MCAO. Photographs of hematoxylin and eosin staining showed that MCAO induced necrotic changes with scalloped shrunken form and apoptotic changes with nuclear chromatin condensations. However, curcumin treatment attenuated MCAO-induced histopathological changes. Moreover, this study clearly showed that peroxiredoxin-5 expression was decreased in MCAO operated animal with vehicle using a proteomics approach. However, this decrease in peroxiredoxin-5 expression was attenuated by curcumin treatment. Reverse-transcription PCR and Western blot analyses confirmed that curcumin treatment alleviated the MCAO injury-induced decrease in peroxiredoxin-5 expression(p<0.05). These results demonstrated that curcumin regulates peroxiredoxin-5 expression in MCAO animal model. In conclusion, our findings suggest that curcumin exerts a neuroprotective effect in cerebral ischemia by attenuating the MCAO-induced decrease in peroxiredoxin-5 expression.

• Journal of Life Science
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• v.27 no.5
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• pp.517-523
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• 2017

This study was to investigate the role of antioxidants on the characteristics of arsenite-damaged boar semen. Collected sperm was diluted with semen extender, and $100{\mu}M$ arsenite was used for sperm damage. Then melatonin, silymarin, curcumin, and vitamin E were applied for 3, 6, and 9 hr in arsenite-treated boar sperm. Sperm characteristics were then analyzed for motility, plasma membrane integrity, mitochondrial activity, and lipid peroxidation. In the results, sperm motility (control, $77.3{\pm}1.8%$) was decreased by arsenite ($33.3{\pm}1.5%$), while the antioxidant treatment groups (100 nM melatonin, $55.8{\pm}3.4%$; $2{\mu}M$ silymarin, $48.8{pm}3.4%$; $10{\mu}M$ curcumin, $53.9{\pm}2.8%$; and $500{\mu}M$ vitamin E, $54.5{\pm}3.1%$) showed increases compared to the arsenite group (p<0.05). $100{\mu}M$ arsenite decreased the sperm plasma membrane integrity ($24.5{\pm}1.6%$) and mitochondrial activity ($58.2{\pm}2.6%$), and increased lipid peroxidation ($5.3{\pm}0.2%$) at 3 hr (p<0.05). However, arsenite-treated samples with 100 nM melatonin, $2{\mu}M$ silymarin, $10{\mu}M$ curcumin, and $500{\mu}M$ vitamin E increased the plasma membrane integrity and mitochondria activity, and decreased lipid peroxidation compared to the arsenite-treated samples. In summary, arsenite may induce sperm damage and oxidation stress, while antioxidants such as melatonin, silymarin, curcumin, and vitamin E are useful for maintaining sperm characteristics. Therefore, antioxidants can protect sperm against damage by arsenite in fresh boar semen.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.4
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• pp.223-233
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• 2021

The objective of this study was to investigate the effect of gypsum on the dry matter yield and the chemical composition of alfalfa in reclaimed tideland with soil dressing. The experimental site was Sukmoon reclaimed tideland. The tideland was reclaimed approximately 17 to 33 years ago and the 70 cm of soil was top-dressed. The soil that covers the reclaimed tideland brought from the island did not treat di-salinized. Treatments were consisted of three groups; control group where no gypsum (G0) was applied and two experimental groups where 2 ton/ha (G2) and 4 ton/ha (G4) of gypsum were applied, respectively. The first harvest was conducted when the alfalfa reached early flowering (open the flower 10%), and after that subsequent harvest was conducted at approximately 35 days intervals. The dry matter yield of the alfalfa showed that G2 was significantly higher in the first year than G0 and G4, and G2 tended to be higher in the second year than G0 and G4, although there were no significant differences between treatments. The reason for the high dry matter yield in G2 was that the soil pH and EC of the soil were at marginal and ideal levels and the coverage and alfalfa botanical composition were also high. In both years, there were no differences in the crude protein, neutral detergent fiber and acid detergent fiber contents and relative feed value between gypsum treatments. Meanwhile, the results in the first and second years showed that the alfalfa dry matter yield were negatively affected by droughts stress in spring and concentrated precipitation in summer. Therefore, this study suggests gypsum treatment in reclaimed tidal land could increase the dry matter yield of alfalfa, and 2 ton/ha of gypsum was the optimum rate.

• Animal cells and systems
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• v.6 no.3
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• pp.215-219
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• 2002

In mid-April, 2000, hundreds of thousands of fish floated dead on the Chungnang River, one of the small branches of the Han River in Seoul. We examined the causes of the accident in detail, through analysis of monitorinq data from the Han River Monitoring Project, which employed the transplanted aquatic moss, Fontinalis antipyretica. This allowed investigation of another possible cause of the fish kill: release of trace metals into the river from industrial sources during the rainfall event. In addition, we aimed to verify the usefulness of aquatic mosses as bioindicators of the event. Water samples collected 48 h after the fish kill exhibited low pH and high Total-N and Total-p, indicating that acidic compounds rich in nitrogen and phosphorus might be a major contaminant. BOD and COD were also very high. On the whole, the conditions of the river water were degraded at that time. Distinct trends were not observed in the chlorophyll phaeophy-tinization quotient and photosynthesis rate of transplanted mosses. How-ever mosses sampled soon after the accident exhibited the lowest values for those variables (P < 0.01), suggesting that stress factors in the river were diluted out over time. Heavy metals with characteristics of industrial effluents (Cr, Pb, Zn, Fe, Cu, and Cd) increased (p ＜ 0.01), indicating that they were unlikely to be major causes of the accident.

• BMB Reports
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• v.47 no.8
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• pp.424-432
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• 2014

The defining feature of Parkinson's disease is a progressive and selective demise of dopaminergic neurons. A recent report on Parkinson's disease animal model demonstrates that poly (ADP-ribose) (PAR) dependent cell death, also named parthanatos, is accountable for selective dopaminergic neuronal loss. Parthanatos is a programmed necrotic cell death, characterized by PARP1 activation, apoptosis inducing factor (AIF) nuclear translocation, and large scale DNA fragmentation. Besides cell death regulation via interaction with AIF, PAR molecule mediates diverse cellular processes including genomic stability, cell division, transcription, epigenetic regulation, and stress granule formation. In this review, we will discuss the roles of PARP1 activation and PAR molecules in the pathological processes of Parkinson's disease. Potential interaction between PAR molecule and Parkinson's disease protein interactome are briefly introduced. Finally, we suggest promising points of therapeutic intervention in the pathological PAR signaling cascade to halt progression in Parkinson's disease.

• KSBB Journal
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• v.19 no.5
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• pp.374-380
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• 2004

Production of therapeutic proteins by transgenic plant cell suspension cultures is an attractive system alternative to the other expression system. However, plant cell cultures have shown low expression level of foreign proteins and decreased cell viability by the changes of culture conditions. Therefore, it is necessary to enhance cell viability during the culture period. In this study, a quantitative analysis technique was designed to measure relative cell viability for plant suspension cells which have cell wall and aggregates. It was found that the programmed cell death of plant cells by apoptosis was essentially linked with the apoptotic pathway of animal cells. Therefore, effects of nicotinamide, 3-aminobenzamide and antioxidants on cell viability and apoptosis were examined in transgenic Nicotiana tabacum cells producing hGM-CSF. With those additives, cell viability could be maintained and apoptosis could be redued. In the result, the extracellular production of hGM-CSF could be enhanced 2.5 fold. It was also found that the supplementation of glutathione and ascorbic acid suppressed both the cold stress-induced decrease in cell viability and the increase of total genomic DNA fragmentation.

• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.23-32
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• 2012

Calcium is an essential nutrient for living organisms, with key structural and signaling roles. Its deficiency in plants can result in poor biotic and abiotic stress tolerance as well as reduced crop quality and yield. Calcium deficiency in humans causes various diseases such as osteoporosis and rickets. Biofortification of calcium in various food crops has been suggested as an economic and environmentally advantageous method to enhance human intake of calcium. Recent efforts to increase the levels of calcium in food crops have used calcium/proton antiporters ($CAXs$) and modified one to increase calcium transport into vacuoles through genetic engineering. It has been reported that overall calcium content of transgenic plants has been increased in their edible portions with some adverse effects. In conclusion, biofortification of calcium will add more value in crops as well as will be beneficial for animal and human. Therefore, more fundamental studies on the mechanisms of calcium ion storage and transporting are essential for more effective calcium biofortification.

• Journal of Microbiology and Biotechnology
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• v.16 no.3
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• pp.349-354
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• 2006

Furaneol, a key aroma compound found in strawberry, pineapple, and processed foodstuffs, has been known to possess various biological activities on animal models. In this study, the antimicrobial effects of furaneol against human pathogenic microorganisms were investigated. The results indicated that furaneol displayed a broad spectrum of antimicrobial activities against Gram-positive and Gram-negative bacteria and fungi without hemolytic activity on human erythrocyte cells. To confirm the antifungal activity of furaneol, we examined the accumulation of intracellular trehalose as a stress response marker on toxic agents and its effect on dimorphic transition of Candida albicans. The results demonstrated that furaneol induced significant accumulation of intracellular trehalose and exerted its antifungal effect by disrupting serum-induced mycelial forms. These results suggest that furaneol could be a therapeutic agent having a broad spectrum of antimicrobial activity on human pathogenic microorganisms.

• Journal of The Korean Society of Agricultural Engineers
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• v.54 no.2
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• pp.55-65
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• 2012

In the winter season, when the ventilation system is operating, the fresh cold air from the slot-type openings of broiler house which directly reached the animal zone can cause various problems such as thermal stress, decreasing of feed and water consumption, occurrence of respiratory disease, and etc. Therefore it is very important to control the trajectory of aero-flow from the slot openings to induce an efficient thermal heat change. Jet-drop distance model was proposed to predict and control the jet-trajectory. However their study was restricted due to the small scaled model and difficulties of measuring the Jet-drop distance. In this study, CFD was applied to analyze qualitatively and quantitatively the jet-drop distance in a real broiler house. The various variables were considered such as installed slot-angle, designed ventilation rate, and the outdoor ambient temperature. From the present study, two linear-regression models using the Jet-drop factor and corrected Archimedes number, and their R-squared values 0.744 and 0.736, respectively, were used. From this study, the applicability of CFD on the analysis of Jet-drop distance model was confirmed.

• Journal of Nutrition and Health
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• v.26 no.9
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• pp.1049-1070
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• 1993

The purpose of this study is to investigate dietary factors which influence the conditions of Korean fatty liver patients. The subjects were 83 fatty liver patients living in city, ages of 10 to 60 yrs old, and they were the same patients studied 9 months ago in previous study. The patients were groupsed into 3 groups(improved, similar, worsened groups) according to the changes in disease condition. Nutrient and alcohol intakes and health status were investigated. In improved group, the patients tended to lose weight, the number of exercising patients was increased, the consumptions of calorie, carbohydrate, calcium, phosphorus were decreased. And alcohol intake was decreased markedly and serum total amino acids(AA), EAA, NEAA, BCAA and AMAA contents, EAA/NEAA and BCAA/AMAA ratios were increased. In worsened group, patients tended to gain weight, the number of exercising patients decreased, the consumptions of calorie, protein, carbohydrate, calcium, phosphorus and niacin decreased but vitamin. A intake increased. And also, in this group, alcohol intake of male patients decreased and that of female slightly increased, and serum EAA, BCAA and AMAA contents, EAA/NEAA and BCAA/AMAA ratios, and total fatty acid contents increased and total AA and NEAA contents decreased. In conclusion, it is desirable to reduced weight, stress, alcohol, salt and animal fat consumptions, and to exercise, and to take adequate amount and quality of protein to improve conditions of fatty liver patients.