KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Antioxidants on Cell Viability and hGM-CSF Production by Transgenic Nicotiana tabacum Suspension Cultures

형질전환된 Nucotiana tabacum 현탁세포배양에서 항산화제가 세포생존도 및 hGM-CSF 생산에 미치는 영향

  • Kim Yong Hoon (Department of Biological Engineering, Inha University) ;
  • Lee Sang Yoon (Department of Biological Engineering, Inha University) ;
  • Kim Dong Il (Department of Biological Engineering, Inha University)
  • 김용훈 (인하대학교 공과대학 생명화학공학부) ;
  • 이상윤 (인하대학교 공과대학 생명화학공학부) ;
  • 김동일 (인하대학교 공과대학 생명화학공학부)
  • Published : 2004.10.01
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Abstract

Production of therapeutic proteins by transgenic plant cell suspension cultures is an attractive system alternative to the other expression system. However, plant cell cultures have shown low expression level of foreign proteins and decreased cell viability by the changes of culture conditions. Therefore, it is necessary to enhance cell viability during the culture period. In this study, a quantitative analysis technique was designed to measure relative cell viability for plant suspension cells which have cell wall and aggregates. It was found that the programmed cell death of plant cells by apoptosis was essentially linked with the apoptotic pathway of animal cells. Therefore, effects of nicotinamide, 3-aminobenzamide and antioxidants on cell viability and apoptosis were examined in transgenic Nicotiana tabacum cells producing hGM-CSF. With those additives, cell viability could be maintained and apoptosis could be redued. In the result, the extracellular production of hGM-CSF could be enhanced 2.5 fold. It was also found that the supplementation of glutathione and ascorbic acid suppressed both the cold stress-induced decrease in cell viability and the increase of total genomic DNA fragmentation.

Keywords

References

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