• 제목/요약/키워드: Animal Agriculture

검색결과 4,415건 처리시간 0.026초

CO-CULTURE OF BOVINE EMBRYOS WITH CUMULUS CELLS

  • Goto, K.;Koba, M.;Takuma, Y.;Nakanishi, Y.;Ogawa, K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제2권4호
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    • pp.595-598
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    • 1989
  • Bovine embryos/ova obtained from in-vitro fertilization were either co-cultured on a monolayer of bovine cumulus cells or cultured in medium alone. Embryos/ova co-cultured with cumulus cells developed to 8-cell (30.9%), morula (29.8%) and blastocyst stages (26.6%) after 3-4, 5-6, and 7-8 days of culture, respectively, while embryos/ova cultured in medium alone failed to develop beyond 8-cell (0-13.3%), morula (0-1.5%) and blastocyst stages (0%). The results of this study demonstrated the beneficial effect of cumulus cells on the development of bovine embryos.

Effects of stocking density on the homeostasis of uric acid and related liver and kidney functions in ducks

  • Peiyi Lin;Sui Liufu;Jinhui Wang;Zhanpeng Hou;Yu Liang;Haiyue Wang;Bingxin Li;Nan Cao;Wenjun Liu;Yunmao Huang;Yunbo Tian;Danning Xu;Xiujin Li;Xinliang Fu
    • Animal Bioscience
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    • 제37권5호
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    • pp.952-961
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    • 2024
  • Objective: Stocking density (SD) is an important issue in the poultry industry, which is related to the production performance, intestinal health and immune status. In the present study, the effects of SD on the metabolism and homeostasis of uric acid as well as the related functions of the liver and kidney in ducks were examined. Methods: A total of 360 healthy 56-day-old Shan-ma ducks were randomly divided into the low stocking density (n = 60, density = 5 birds/m2), medium stocking density (n = 120, density = 10 birds/m2) and high stocking density groups (HSD; n = 180, density = 15 birds/m2). Samples were collected in the 3rd, 6th, and 9th weeks of the experiment for analysis. Results: The serum levels of uric acid, lipopolysaccharide and inflammatory cytokines (interleukin-1β [IL-1β], IL-8, and tumor necrosis factor-α [TNF-α]) were increased significantly in the HSD group. Serious histopathological lesions could be seen in both the livers and kidneys in the HSD group in the 9th week. The mRNA expression levels of inflammatory cytokines (IL-8 and TNF-α) and related pathway components (toll-like receptor 4, myeloid differentiation primary response gene 88, and nuclear factor-κB) were increased significantly in both the livers and kidneys in the HSD group. The mRNA expression levels of enzymes (adenosine deaminase, xanthine oxidase, phosphoribosyl pyrophosphate amidotransferase, and phosphoribosyl pyrophosphate synthetase 1) related to the synthesis of uric acid increased significantly in the livers in the HSD group. However, the mRNA expression level of solute carrier family 2 member 9, which plays an important role in the excretion of uric acid by the kidney, was decreased significantly in the kidneys in the HSD group. Conclusion: These results indicated that a higher SD could cause tissue inflammatory lesions in the liver and kidney and subsequently affect the metabolism and homeostasis of uric acid, and is helpful for guiding decisions related to the breeding and production of ducks.

NORMALITIES OF CALVES OBTAINED FROM THE TRANSFERS OF BLASTOCYSTS PRODUCED BY TOTALLY IN-VITRO TECHNIQUE

  • Goto, K.;Kajihara, Y.;Kosaka, S.;Koba, M.;Nakanishi, Y.;Ogawa, K.;Oku, T.;Fujiyama, M.;Yoshida, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • 제2권4호
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    • pp.591-593
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    • 1989
  • Bovine blastocysts were obtained by totally in-vitro technique and then transferred to recipient cows. Total of 15 calves (including 4 premature calves) were obtained from 11 recipients. Four calves were obtained from the transfers of fresh blastocysts and 9 calves were obtained from the transfers of frozen-thawed blastocysts. Two calves were obtained from the bisected fresh blastocyst. The males and 5 females were delivered. Birth weight of calves was within normal range except one female, and all calves appeared to be completely normal.

In Vitro Maturation of Tiger Oocytes: Case Report

  • Lee, Hyosang;Yin, Xijun;Lee, Youngho;Jeon, Sejin;Suh, Yongil;Jo, Sujin;Choi, Eugene;Min, Wongi;Oh, Donghan;Kong, Ilkeun
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2004년도 춘계학술발표대회
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    • pp.200-200
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    • 2004
  • The purpose of this study was carried out to determine the possibility of in vitro maturation of tiger oocytes. Immature oocytes were recovered from a pairs of ovaries. A total of 78 oocytes were collected, of which forty threes were identified as compact cumulus cells and uniform cytoplasm. 43 COCs were in vitro matured at 39℃, 5% CO₂ in air atmosphere for 48 h in a IVM medium (TCM-199 supplement with 10% FBS, 0.6 mM cysteine, 0.2 mM pyruvic acid and 10 IU/㎖ HMG). (omitted)

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Clip ligation for treatment of patent ductus arteriosus occlusion in three cats

  • Ozai, Yusuke;Uemura, Akiko;Tanaka, Ryou;Takeuchi, Aki;Hamabe, Lina;Shimada, Kazumi;Yokoi, Aimi;Hirose, Miki;Watanabe, Momoko;Uehara, Katsumi
    • Journal of Veterinary Science
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    • 제23권4호
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    • pp.39.1-39.5
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    • 2022
  • Patent ductus arteriosus (PDA) is a rare congenital cardiovascular anomaly in cats. Due to their small body, intercostal thoracotomy is the most common option to close the PDA. However, few reports detail the surgical technique for ligating PDA in kittens. In this case report, three cats weighing 1.4 kg, 1.2 kg, and 2.9 kg were diagnosed PDA. Clip ligation via left fourth intercostal thoracotomy was performed and the cats were successfully treated. Postoperative echocardiography showed no residual flow in any of the cases. This case report highlights clip occlusion for small cats with PDA could be safe and effective.

New Evidence of Alleles (V199I and G52S) at the PRKAG3 (RN) Locus Affecting Pork Meat Quality

  • Chen, J.F.;Dai, L.H.;Peng, J.;Li, J.L.;Zheng, R.;Zuo, B.;Li, F.E.;Liu, M.;Yue, K.;Lei, M.G.;Xiong, Y.Z.;Deng, C.Y.;Jiang, S.W.
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권4호
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    • pp.471-477
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    • 2008
  • The porcine PRKAG3 (RN) gene encodes the regulatory gamma subunit of adenosine monophosphate-activated protein kinase (AMPK), which is a good candidate gene affecting meat quality. In this study, the effects of two missense mutations A595G (Ile199Val) and G154A (Gly52Ser) in porcine PRKAG3 gene on meat quality traits were studied in M. Longissimus dorsi (LD), M. Semispinalis capitis (SC) and M. Biceps femoris (BF) from different populations of 326 pigs. The PRKAG3 alleles 199I, 199IV, 52S and 52G were identified with PCR-RFLPs and all genotypes - 199I/199I, 199I/199V, 199V/199V, 52S/52S, 52S/52G and 52G/52G - were found. The frequency of V allele was larger than that of I allele in all populations. I allele frequency was zero in Chinese Meishan pigs (population D) especially. G allele frequency was larger than that of S allele in all populations except Large White (population A). Both variations at the PRKAG3 locus significantly affected these meat quality traits. The pork meat quality has not previously been established in Meishan or crosses thereof. The results suggested that generally pH of LD, SC and BF was higher in Meishan pigs than that in other populations. Moreover, Meishan pigs showed higher water-holding capacity and intramuscular fat (IMF), lower water content and water loss percentage compared to other populations in terms of the two variations. The results present here supply new evidence that alleles V199I and G52S at the PRKAG3 locus affect pork meat quality and provide useful information on pork production.

Effect of Bovine Somatotropin (bST) Treatment on Progesteron Concentration and Pregnancy Rate in Hanwoo

  • S. H. Jung;Lee, J. W.;B. H. Son;I. H. Bae;S. G. Cho;I. K. Kong
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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    • pp.14-14
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    • 2002
  • The purpose of this study was to determine the effect of bST treatment on progesteron concentration, embryo recovery and pregnancy rate following embryo transfer. Donor cows were superovulated with Folltropin-V and PGF₂ α combination method and then inseminated with frozen semen 3 times 12 hrs interval. Donor and recipient cows were assigned to control and bST group, of which was given a single injection of bST (500 ㎎, sc) at insemination or estrus detection. Embryo collection of superovulated cows were flushed nonsurgical method at 7 to 8 days after artificial insemination. (omitted)

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