• KSBB Journal
• /
• 제19권1호
• /
• pp.42-49
• /
• 2004

Racemic epoxide에 대한 입체선택적 가수분해능을 가지고 있는 곰팡이, Aspergillus niger LK로부터 epoxide hydrolase (EH, EC 3.3.2.3) 유전자의 진화적 유연관계 분석을 행하였다. A. niger LK의 EH 염기서열로부터 유추한 EH 단백질 아미노산 서열은 여러 박테리아의 EH들 및 포유동물의 microsomal EH들과 유의적인 유사성을 가지고 있었으며 a/$\beta$ hydrolase fold family에 속하였다. A. niger LK의 EH 단백질의 입체구조예측은 Protein Data Bank에 수록된 lqo7의 3D 결정구조와 90.6% identity를 가지는 것으로 나타났으며 다른 EH들의 아미노산 서열비교를 행한 결과 Asp$^{192}$ , Asp$^{348}$ 및 His$^{374}$ 이 catalytic triad를 구성하고 있는 것으로 추정되었다. 여러 생물종의 EH서열을 기능적 및 구조적 domain 서열을 기초로 하여 multiple sequence alignment를 행하고 Neighbor-Joining/UPGMA method를 이용하여 계통수를 복원한 결과 다른 생물종들의 EH와의 진화거리는 서로 1.841∼2.682로 멀었으나 EH의 기능을 가지기 위한 oxyanion hole 및 a/$\beta$ hydrolase fold family의 catalytic triad는 잘 보존되고 있어 공통조상으로부터 진화되어 왔음을 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권9호
• /
• pp.1393-1400
• /
• 2018

Objective: This study was carried out to assess the haplotype diversity and population dynamics in cattle populations of Ethiopia. Methods: We sequenced the complete mitochondrial cytochrome b gene of 76 animals from five indigenous and one Holstein Friesian${\times}$Barka cross bred cattle populations. Results: In the sequence analysis, 18 haplotypes were generated from 18 segregating sites and the average haplotype and nucleotide diversities were $0.7540{\pm}0.043$ and $0.0010{\pm}0.000$, respectively. The population differentiation analysis shows a weak population structure (4.55%) among the populations studied. Majority of the variation (95.45%) is observed by within populations. The overall average pair-wise distance ($F_{ST}$) was 0.049539 with the highest ($F_{ST}=0.1245$) and the lowest ($F_{ST}=0.011$) $F_{ST}$ distances observed between Boran and Abigar, and Sheko and Abigar from the indigenous cattle, respectively. The phylogenetic network analysis revealed that all the haplotypes detected clustered together with the Bos taurus cattle and converged to a haplogroup. No haplotype in Ethiopian cattle was observed clustered with the reference Bos indicus group. The mismatch distribution analysis indicates a single population expansion event among the cattle populations. Conclusion: Overall, high haplotype variability was observed among Ethiopian cattle populations and they share a common ancestor with Bos taurus.

• ALGAE
• /
• 제17권1호
• /
• pp.59-68
• /
• 2002

A phylogenetic affinity between charophytes and embryophytes (land plants) has been explained by a few chloroplast genomic characters including gene and intron (Manhart and Palmer 1990; Baldauf et al. 1990; Lew and Manhart 1993). Here we show that a charophyte, Spirogyra maxima, has the largest operon of angiosperm chloroplast genomes, rpl23 operon (trnⅠ-rpl23-rpl2-rps19-rpl22-rps3-rpl16-rpl14-rps8-infA-rpl36-rps11-rpoA) containing both embryophyte introns, rpl16.i and rpl2.i. The rpl23 gene cluster of Spirogyra contains a distinct eubacterial promoter sequence upstream of rpl23, which is the first gene of the green algal rpl23 gene cluster. This sequence is completely absent in angiosperms but is present in non-flowering plants. The results imply that, in the rpl23 gene cluster, early charophytes had at least two promoters, one upstream of trnⅠ and and another upstream of rpl23, which partially or completely lost its function in land plants. A comparison of gene clusters of prokaryotes, algal chloroplast DNAs and land plant cpDNAs indicated a loss of numerous genes in chlorophyll a+b eukaryotes. A phylogenetic analysis using presence/absence of genes and introns as characters produced trees with a strongly supported clade containing chlorophyll a+b eukaryotes. Spirogyra and embryophytes formed a clade characterized by the loss of rpl5 and rps9 and the gain of trnⅠ (CAU) and introns in rpl2 and rpl16. The analyses support the hypothesis that the rpl23 gene cluster and the rpl2 and rpl16 introns of land plants originated from a common ancestor of Spirogyra and land plants.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권7호
• /
• pp.903-909
• /
• 2004

The variability of mtDNA hypervariable segment I (HVS I) sequences was investigated in a total of 48 birds belonging to 12 Chinese native chicken breeds. Sixteen haplotypes were identified from 35 polymorphic nucleotide sites which accounted for 6.4% of a sequenced 544 bp fragment. Diversity analysis of the haplotypes showed that Tibetan, Langshan and Henan cockfight chicken had only one haplotype, while ancient haplotypes existed in Taihe silky and Chahua chicken. Phylogenetic analysis of the haplotypes suggested that Chinese native chicken breeds shared 5 maternal lineages and some breeds would share the same maternal lineage, regardless of their external features and ecological types. Both divergent and phylogenetic analysis of the haplotypes indicated the close genetic relationships between the Chinese native chicken breeds and G. g. gallus and G. g. spadiceus from different areas, which implied that G. g. gallus and G. g. spadiceus were the original ancestors of the Chinese native chicken breeds.

• Asian-Australasian Journal of Animal Sciences
• /
• 제10권6호
• /
• pp.614-620
• /
• 1997

Pedigree data were used to predict the performance of 1,166 imported Americas Brahman breeder cattle, composed of 104 bulls and 1,062 heifers with an average age of 24.4 months (range of 17 to 40 months). A total of 13 full sib, 10 maternal half sib, and 228 paternal half sib groups were identified, each with average group size of 2.08, 2.00, and 4.49, respectively. Only 758 (64.9% of total) imported cattle were found to have at least one ancestor with expected progeny differences (EPDs) reported in the Spring 1995 Brahman Sire Summary. Moderate average accuracy values of .71, .69, .52, and .52 for birth weight, weaning weight, yearling weight, and maternal milk, respectively, were noted for EPDs of the ancestors. Prediction equations were derived by multiple regression analysis of available EPDs of sire, paternal grand sire, and maternal grand sire. Based on pedigree indexes that involve various combinations of available ancestral information, the average predicted EPDs (lbs) for imported cattle were $1.76{\pm}0.54$, $14.93{\pm}4.86$, $25.10{\pm}9.50$, and $5.86{\pm}2.08$ for birth weight, weaning weight, yearling weight, and maternal milk, respectively. Significant correlations (p < .05) were also found between sire and son EPDs (+.27) for yearling weight; and between sire and paternal grand sire EPDs for birth weight (+.34), weaning weight (+.51), yearling weight (+.49), and maternal milk (+.55).

• 한국멀티미디어학회논문지
• /
• 제9권10호
• /
• pp.1257-1265
• /
• 2006

XML의 사용이 증가함에 따라서 XML과 관련된 다양한 분야에 대한 연구의 필요성도 증대되고 있는데, 특히 XML로 표현된 문서를 효과적으로 저장, 관리, 검색하기 위한 XML 문서 관리 시스템에 대한 많은 연구들이 활발하게 진행되어 왔다. 이러한 시스템들 중 BitCube는 검색에서 빠른 속도를 통해 뛰어난 성능을 입증한 3차원 비트맵 인덱스 시스템이다. 그러나 문서, 경로, 단어의 세 축으로 이루어진 3차원 메모리 구조를 사용하기 때문에 클러스터 내의 문서가 증가함에 따라 인덱스의 크기가 급격하게 증가하고, 이로 인해 연산 수행속도가 저하되는 문제점을 가지고 있다. 본 논문에서는 BitCube시스템의 문제점을 개선하기 위해 XML 문서의 경로 통합 기법을 제안한다. 이 기법은 XML문서를 시스템의 하부구조에 사용되는 관계형 DBMS에 저장할 때, 유사한 경로들을 통합하여 저장하고, 기존의 3차원 비트맵 인덱스에서 단어로 구성했던 한 축을 value 단위로 구성하여 인덱스 크기를 줄인다 제안한 방법은 3차원 비트맵 인덱스를 구성할 때 인덱스의 크기를 줄이고, 이로 인하여 검객 속도 향상과 디스크 저장 공간의 효율을 가진다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권6호
• /
• pp.876-887
• /
• 2015

Dicer, an ribonuclease type III type endonuclease, is the key enzyme involved in biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs), and thus plays a critical role in RNA interference through post transcriptional regulation of gene expression. This enzyme has not been well studied in the Indian water buffalo, an important species known for disease resistance and high milk production. In this study, the primary coding sequence (5,778 bp) of bubaline dicer (GenBank: AB969677.1) was determined and the bubaline Dicer1 biocomputationally characterized to determine the phylogenetic signature among higher eukaryotes. The evolutionary tree revealed that all the transcript variants of Dicer1 belonging to a specific species were within the same node and the sequences belonging to primates, rodents and lagomorphs, avians and reptiles formed independent clusters. The bubaline dicer1 is closely related to that of cattle and other ruminants and significantly divergent from dicer of lower species such as tapeworm, sea urchin and fruit fly. Evolutionary divergence analysis conducted using MEGA6 software indicated that dicer has undergone purifying selection over the time. Seventeen divergent sequences, representing each of the families/taxa were selected to study the specific regions of positive vis-$\grave{a}$-vis negative selection using different models like single likelihood ancestor counting, fixed effects likelihood, and random effects likelihood of Datamonkey server. Comparative analysis of the domain structure revealed that Dicer1 is conserved across mammalian species while variation both in terms of length of Dicer enzyme and presence or absence of domain is evident in the lower organisms.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권1호
• /
• pp.12-23
• /
• 2020

Objective: The objective of a conservation program is to maintain maximum genetic diversity and preserve the viability of a breed. However, the efficiency of a program is influenced by the ability to accurately measure and predict genetic diversity. Methods: To examine this question, we conducted a simulation in which common measures (i.e. heterozygosity) and novel measures (identity-by-descent probabilities and parental genomic components) were used to estimate genetic diversity within a conserved population using double-labeled single nucleotide polymorphism markers. Results: The results showed that the accuracy and sensitivity of identity-by-state probabilities and heterozygosity were close to identity by descent (IBD) probabilities, which reflect the true genetic diversity. Expected heterozygosity most closely aligned with IBD. All common measures suggested that practices used in the current Chinese pig conservation program result in a ~5% loss in genetic diversity every 10 generations. Parental genomic components were also analyzed to monitor real-time changes in genomic components for each male and female ancestor. The analysis showed that ~7.5% of male families and ~30% of female families were lost every 5 generations. After 50 generations of simulated conservation, 4 male families lost ~50% of their initial genomic components, and the genomic components for 24.8% of the female families were lost entirely. Conclusion: In summary, compared with the true genetic diversity value obtained using double-labeled markers, expected heterozygosity appears to be the optimal indicator. Parental genomic components analysis provides a more detailed picture of genetic diversity and can be used to guide conservation management practices.

• 대한지구과학교육학회지
• /
• 제5권2호
• /
• pp.139-147
• /
• 2012

The purpose of this study was to explain how extraordinary the scientific technology or our ancestor was from the modern perspective by remodeling the most unique astronomical instrument, So-ganui (小簡儀), developed in the Sejong Period (世宗時代) after being examined with contemporary and the principles of the science and observational technology would be properly understood and measured directly. When measuring the altitude of the sun and azimuth using So-ganui, it was adjusted with the horizontal coordinate system and measured using Jipyeonghwan (地平環), Ipeunhwan (立運環) and Guyhyeong (窺衡). Based such measuring principles, the measurement accuracy proposed using So-ganui are as follows. The remodeled So-ganui produced approximately ${\pm}0.29$ degrees error on average at high altitude while in measuring the azimuth degrees, there was difference of ${\pm}0.35$ degrees. Since the theoretically, the measurement error for So-ganui was ${\pm}0.5$ degrees, the remodeled So-ganui could accurately measure at the high altitude compared to So-ganui from the Sejong period. In the study, So-ganui, which has disappeared, has been remodeled in modern perspective to be used as the educational material to accurately understand the principles of science and measurement technology from the Sejong period. The findings could contribute to raising the reputation in the astronomical observations from the documents from the Sejong period. Furthermore, this study has materialized the celestial and sky our ancestors have viewed with the observational principles of their times, on the computer screen via a webcam, bringing out interest in the traditional science for the students.

• 동의생리병리학회지
• /
• 제22권5호
• /
• pp.1078-1105
• /
• 2008

An empirical formula that has been empirically shown to be effective for a particular condition can be said to add medicinal materials to an existing formula or to make a new prescription by the Korean medical doctors' empirical clinic. This dissertation aims to study the prescriptions according to A Confidential Document of A Distinguished Medical Family written by the writer's ancestor and to contribute to the development of Korean medicine. A Confidential Document of A Distinguished Medical Family is a medicinal recipe that is an heirloom of the Gyeongju Kim family for the eighth generation. And the date and the writer of the book are unknown. The contents of the book consist of 18 parts and are made up of about 300 medical recipes. Of these, 158 formulas and indications of cold damage, wind, urination, stool, jaundice, and internal injury are found in the book. From among these, 61 formulas was directly quoted out of The Precious Mirror of Oriental Medicine, 97 formulas are adjusted in proportion or combined. By the parity of reasoning of the above, I think that this book was published after The Precious Mirror of Oriental Medicine. I will continue to study other formulas except the formulas above mentioned.