• Journal of the Korean Geotechnical Society
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• v.26 no.6
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• pp.57-70
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• 2010

Recent earthquakes have demonstrated that the tunnels, which were once considered to be highly resistant to earthquakes, are susceptible to substantial damage under severe seismic loading. Among various modes of deformation under an earthquake loading, the response of the tunnel in the transverse direction is known to be the critical mode. This paper investigates the seismic response of the tunnel in the transverse direction using the method of seismic displacement, which is a type of pseudo-static analysis. Firstly, the methods of calculating the ground deformation are compared. It is shown that the single and double cosine may not provide an accurate estimation of the ground deformation, and that a one-dimensional site response analysis needs to be performed for a more reliable evaluation. Secondly, the tunnel responses are calculated using the simplified, analytical, and numerical solutions. It is demonstrated that the simplified method provides poor estimates of the tunnel response ground deformation. The analytical solution is shown to be effective in modeling circular tunnels in uniform ground, but has serious limitation in modeling tunnel response in non-uniform ground. Numerical analyses are shown to be applicable to all cases, and give the most accurate estimates of the tunnel response. It is also demonstrated that the linear solutions can be so conservative that the soil nonlinearity needs to be accounted for more accurate evaluation of the tunnel response.

• Analytical Science and Technology
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• v.19 no.6
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• pp.482-489
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• 2006

Two electrode-based lactate biosensor was prepared by immobilizing lactate oxidase (LOD) obtained from pediococcus species in a poly(vinyl alcohol). Hydrogen peroxide ($H_2O_2$) produced by the reaction of lactate and LOD was detected on the Pt-black that was electrochemically deposited on the Au electrode. Sensors fabricated with Pt-black deposited Au electrode provided a high current of $H_2O_2$ oxidation at a substantially lowered applied potential (+300 mV vs. Ag/AgCl), resulting in reduced interferences from easily oxidizable species such as ascorbic acid, acetaminophen, and uric acid. An outer membrane is formulated by adjusting water uptake of hydrophilic polyurethane (HPU). The sensor performance was evaluated in vitro with both flow-through arrangement and static mode. The sensor showed a linear range from 0.1 mM to about 9.0 mM in 0.05 M phosphate buffer (pH 7.6) containing 0.05 M NaCl. Storing the sensors prepared in this work at $4^{\circ}C$ buffer solution while not in use, they provided same electrochemical performance for more than 25 days.

• Analytical Science and Technology
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• v.21 no.1
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• pp.34-40
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• 2008

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of lercanidipine in human plasma. After addition of internal standard (amlodipine), plasma was precipitated with acetonitrile and the supernatant was evaporated. The residues were dissolved in 50 % acetonitrile and analyzed by LC-MS/MS. Using MS/MS with multiple reaction monitoring(MRM) mode, lercanindipine were selectively detected without severe interference from human plasma. The standard calibration curve for lercanidipine was linear (r = 0.9994) over the concentration range 0.05-20.0 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of lercanidipine was lower than 11.7 % (correlation of variance, CV), and accuracy was between 94.4-114.8 %. This method has been successfully applied to the pharmacokinetic study of lercanidipine in human plasma.

• Analytical Science and Technology
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• v.20 no.2
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• pp.138-146
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• 2007

A simple and sensitive method for the determination of paroxetine in canine plasma was developed and validated by liquid-liquid extraction and liquid chromatography-tandem mass spectrometry (LC-/MS/MS). Fluoxetine was used as an internal standard. Paroxetine and internal standard in plasma samples were extracted using TBME (tert-butyl methyl ether). A centrifuged upper layer was then evaporated and reconstituted with mobile phase of 50% acetonitrile adjusted to pH 3 by formic acid. The reconstituted samples were injected into a Capcell Pak UG120 ($2.0{\times}150mm$, $5{\mu}m$) column. Using MS/MS with SRM (selective reaction monitoring) mode, the transitions (precursor to product) monitored were m/z $330{\rightarrow}192$ for paroxetine, and m/z $310{\rightarrow}148$ for internal standard. Linear detection responses were obtained for paroxetine concentration range of 0.02~5 ng/mL. A correlation coefficient of linear regression ($R^2$) was 0.9993. Detection of paroxetine in canine plasma was accurate and precise, with limit of quantification at 0.02 ng/mL. The method has been successfully applied to pharmacokinetic study of paroxetine in healthy beagle dogs.

• Analytical Science and Technology
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• v.22 no.6
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• pp.480-487
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• 2009

The herbicide dicamba (2-methoxy-3,6-dichlorobenzoic acid) in soil and plants was determined by gas chromatography-mass spectrometry (GC/MS). The samples were extracted with diethyl ether at pH 2, and washed with 0.1 N HCl, and then dried. The dried residue was derivatized in 1 mL of 10% $H_2SO_4$-MeOH for 2 hr at $80^{\circ}C$. The reaction mixture was neutralized with 4 mL of sodium bicarbonate solution and reextracted with 5 mL of diethyl ether. After the extract was concentrated, dicamba was determined by GC/MS-SIM mode. There was good linearity above 0.999 in the ranges of the $1.0{\sim}100{\mu}g/kg$. Total 42 sample including 32 soil samples and 10 plants samples were analyzed by developed method. Dicamba was detected in the concentration range of $2.9-123.9{\mu}g/kg$ in 15 samples among 32 soil samples and in the concentration range of $43-33,252{\mu}g/kg$ in 5 samples among 10 plants samples. A cause of the wither and die of the pine trees is suspected to spray dicamba around or directly to them.

• Journal of the Korean Chemical Society
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• v.55 no.3
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• pp.472-477
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• 2011

The concentration of selenium in grain samples was determined using isotope dilution method in ORC-ICPMS. The experimental conditions were optimized to $H_2$ mode and the flow rate was $4.0\;mL\;min^{-1}$. ORC in $H_2$ mode proved to eliminate most of polyatomic interferences except $BrH^+$ when Br is present in sample matrix. Chemical removal of Br was very difficult and the mathematical correction was successfully employed. The fraction of $BrH^+$ generated from Br at the current experimental condition was 14.1%. The signal on m/z 82 was corrected and calculated for isotope dilution. The analytical reliability of the propose method was successfully evaluated by analyzing the certified standard reference material NIST SRM 1566 and 1567. The method was applied to real samples and the results are $0.034{\pm}0.001\;{\mu}g\;g^{-1}$ for white rice, $0.059{\pm}0.002_5\;{\mu}g\;g^{-1}$ for brown rice, $0.029{\pm}0.001_4\;{\mu}g\;g^{-1}$ for black rice, and $0.034{\pm}0.002\;{\mu}g \;g^{-1}$ for barley. The detection limits ($3\sigma$) for Se was $0.012\;ng\;g^{-1}$.

• Korean Journal of Food Science and Technology
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• v.39 no.2
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• pp.122-127
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• 2007

In this study, an analytical method was optimized for detecting dioxin-like PCBs in fish and shellfish. Here, homogenized samples were extracted using an accelerated solvent extraction (ASE) system with 33 mL cell size. Multilayer column chromatography, which consisted of acidic, basic and neutral silica gels, was used for the clean up of the extracts. The instrumental analysis was executed by HRGC/HRMS to a resolution of 10,000 using 4 window multiple ion detection (MID) mode. For the results, the average recoveries ranged from 94.1 to 104.1% (${\pm}8.4$) and the limit of detection was approximately 0.1 pg/g at S/N ratio >3. Finally, the detected concentrations of dioxin-like PCBs for fish and shellfish were in the range of 0.030-1.836 pg TEQ/g.

• Journal of Korean Society of Steel Construction
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• v.18 no.4
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• pp.503-514
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• 2006

FBG sensors are capable of measuring the strain of structures easily and more durably than electric resistance gauges. Thus, many researches are dedicated to the application for the response monitoring or non-destructive evaluation of structures using FBG sensors. Additionally, the measured strains at the top and bottom of a cross-section can be transformed into the curvature of the section, which can be used to calculate its vertical displacement. Hence, this study aims to measure the dynamic strain signals of a steel section simply supported beam and to estimate the dynamic displacement from the strain signals, after which the estimated displacement is com pared with the measured displacement. The dynamic characteristics (natural frequency, damping ratio and mode shape) of the beam are predicted from both the estimated and measured displacement signals, and from the strain time history of the FBG sensors. The predicted properties are compared with those of an analytical model of the beam. The estimated displacement. However, the predicted dynamic properties from both the estimated displacements and the measured strains are well-correlated with those from the measured displacement. It is therefore appreciated that the estimation of the dynamic properties of FBG sensor signals is reasonable. Especially, the strain signal of the FBG sensor was amplified at a higher-frequency region in comparison with the displacement estimation with higher-mode properties.

• Journal of Korean Society of Steel Construction
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• v.21 no.1
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• pp.63-70
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• 2009

Failure modes result in fracture or tearing, which may cause deterioration of resistance and reduction of inelastic deformation capacity. The potential failure modes for Special Concentrically Braced Frames (SCBFs) include fracture or tearing of the brace, net section fracture of the brace or gusset plate, fracture of the gusset plate welds, shear fracture of the bolts, block shear, excessive bolt bearing deformation, and buckling of the gusset plate. HSS tubular braces are commonly used in SCBFs, and net section fracture of the tubular brace may also occur through the brace net section at the end of the slot cut into the tube to slip over the gusset plate. This failure mode is categorized as a tension failure mode, and may cause dramatic loss of resistance and brittle behavior. Net section reinforcement is required according to AISC design specifications (AISC 2001). In this paper, the need to reinforce the net section area was discussed. Initially, the results of the net section fracture tests done by the University of California in Berkeley were presented with the modeling of these tests using FE models. To investigate the possibility of net section fracture in an actual frame, the slot end hole model was adapted to the frame FE model, and alternate near-fault histories were applied with tension-dominated cycles, since previous analyses showed that loading history was the most critical factor in net section fracture. The need for this reinforcement (cover plate) and the tension-dominated near-fault history were investigated.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.27 no.2
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• pp.63-70
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• 2014

Design sensitivity analysis and topology design optimization for a piezoelectric crystal resonator are developed. The piezoelectric crystal resonator is deformed mechanically when subjected to electric charge on the electrodes, or vice versa. The Mindlin plate theory with higher-order interpolations along thickness direction is employed for analyzing the thickness-shear vibrations of the crystal resonator. Thin electrode plates are masked on the top and bottom layers of the crystal plate in order to enforce to vibrate it or detect electric signals. Although the electrode is very thin, its weight and shape could change the performance of the resonators. Thus, the design variables are the bulk material densities corresponding to the mass of masking electrode plates. An optimization problem is formulated to find the optimal topology of electrodes, maximizing the thickness-shear contribution of strain energy at the desired motion and restricting the allowable volume and area of masking plates. The necessary design gradients for the thickness-shear frequency(eigenvalue) and the corresponding mode shape(eigenvector) are computed very efficiently and accurately using the analytical design sensitivity analysis method using the eigenvector expansion concept. Through some demonstrative numerical examples, the design sensitivity analysis method is verified to be very efficient and accurate by comparing with the finite difference method. It is also observed that the optimal electrode design yields an improved mode shape and thickness-shear energy.