• Title/Summary/Keyword: Analytical Tools

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QUICK DETERMINATION OF MEAT COLOR, METMYOGLOBIN FORMATION AND LIPID OXIDATION IN BEEF, PORK AND CHICKEN BY NEAR-INFRARED SPECTROSCOPY

  • Mitsumoto, Mitsuru;Sasaki, Keisuke;Murakami, Hitoshi
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1259-1259
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    • 2001
  • Meat becomes brown and rancid during storage in the refrigerator and display in the case. Color changes, metmyoglobin formation and lipid oxidation are the important problems in the transportation / distribution of meat and retail display. The freshness of meat is determined by the sense of vision and smell. Since conventional method determining lipid oxidation is time consuming and destructive (it needs to homogenize meat with reagents, filtrate, time for reaction and read optical density using spectroscopy), more rapid and nondestructive technical tools are desired. The objective of this work was to evaluate near-infrared spectroscopy as an analytical tool for determining meat color, metmyoglobin formation and lipid oxidation. in beef, pork and chicken. Semitendinosus and longissimus thoracis muscles from six beef steers, biceps femoris and longissimus thoracis muscles from twelve LWD crossbred pigs, and superficial pectoral muscles from twenty-four broilers were used. About a 5-cm diameter and 1-cm thick sample (20.0g) was cut from the muscle and placed on plastic foam, over-wrapped with PVC film, and displayed under flourescent lights at 4 degrees C. during 10 days for beef and pork or 4 days for chicken. The spectra was measured by NIR systems Model 5500 Spectrophotometer using fiber optic scan at range of 400 - 1100 nm. Data were recorded at 2 nm intervals and 10 scans / 10 sec were averaged for every sample. Data obtained were saved as log 1/Re, where Re is the reflectance energy, and then mathematically transformed to second derivatives to reduce effects of differences in particle size. $L^{*}$, $a^{*}$ and $b^{*}$, and metmyoglobin formation were determined by conventional spectrophotometer using the integrating sphere unit. 2-Thiobarbituric acid reactive substances (TBARS) were measured for lipid oxidation. A multiple linear regression was used to find the equation which would best fit the data. The number of wavelengths used in the equation was selected based on the fewer number compared to the increasing multiple correlation and Decreasing standard error. (omitted)

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Structure and action mechanism of humic substances for plant stimulations

  • Jeon, Jong-Rok;Yoon, Ho Young;Shin, Gyeong-Im;Jeong, Song Yi;Cha, Joon-Yung;Kim, Woe-Yeon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.3
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    • pp.175-179
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    • 2018
  • Humic substances that can be obtained from coal resources such as leonardite in a bulk scale have been employed as crop stimulators and soil conditioners. The polymeric organics containing a variety of aromatic and aliphatic structures are known to activate plants in a multifunctional way, thus resulting in enhanced germination rate and abiotic stress resistance concomitant with induction of numerous genes and proteins. Although detailed structural-functional relationship of humic substances for plant stimulations has not been deciphered yet, cutting-edge analytical tools have unraveled critical features of humic architectures that could be linked to the action mechanisms of their plant stimulations. In this review article, we introduce key findings of humic structures and related biological functions that boost plant growth and abiotic stress resistance. Oxygen-based functional groups and plant hormone-like structures combined with labile and recalcitrant carbon backbones are believed to be critical moieties to induce plant stimulations. Some proteins such as HIGH-AFFINITY $K^+$ TRANSPORTER 1, phospholipase A2 and $H^+$-ATPase have been also recognized as key players that could be critically involved in humic substance-driven changes in plant physiology.

GUI construction for 3D visualization of ocean hydrodynamic models (해수유동모델의 3차원 가시화를 위한 GUI 구축)

  • Lee, Won-Chan;Park, Sung-Eun;Hong, Sok-Jin;Oh, Hyun-Taik;Jung, Rea-Hong;Koo, Jun-Ho
    • Proceedings of KOSOMES biannual meeting
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    • 2006.11a
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    • pp.213-215
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    • 2006
  • This study presents an application of GIS technologies to construct the graphic user interface for 3-dimensional exhibition of the results obtained by ocean hydrodynamic model. In coastal management studies, GIS provide a receptacle for scattered data from diverse sources and an improvement of the 3D visualization of such data. Within the frame of a GIS a variety of analytical, statistical and modeling tools can be applied to transform data and make them suitable for a given application. A 3D hydrodynamic model was driven by time-dependent external forcing such as tide, wind velocity, temperature, salinity, river discharge, and solar radiation under the open boundary condition. The Jinhae Bay was selected as a case study. Here, we have used GeoMania v2.5 GIS software and its 3D Analyst extension module to visualize hydrodynamic model result that were simulated around the Jinhae Bay.

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Fabrication of Transition-metal-incorporated TiO2 Nanopowder by Flame Synthesis (화염법에 의한 천이금속 첨가 이산화티타늄 나노분말의 제조)

  • Park Hoon;Jie Hyunseock;Lee Seung-Yong;Ahn Jae-Pyoung;Lee Dok-Yol;Park Jong-Ku
    • Journal of Powder Materials
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    • v.12 no.6 s.53
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    • pp.399-405
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    • 2005
  • Nanopowders of titanium dioxide $(TiO_2)$ incorporating the transition metal element(s) were synthesized by flame synthesis method. Single element among Fe(III), Cr(III), and Zn(II) was doped into the interior of $TiO_2$ crystal; bimetal doping of Fe and Zn was also made. The characteristics of transition-metal-doped $TiO_2$ nanopowders in the particle feature, crystallography and electronic structures were determined with various analytical tools. The chemical bond of Fe-O-Zn was confirmed to exist in the bimetal-doped $TiO_2$ nanopowders incorporating Fe-Zn. The transition element incorporated in the $TiO_2$ was attributed to affect both Ti 3d orbital and O 2p orbital by NEXAFS measurement. The bimetal-doped $TiO_2$ nanopowder showed light absorption over more wide wavelength range than the single-doped $TiO_2$ nanopowders.

Usage of Internet-based Oceanographic GIS of the NW Pacific for Joint Analysis of Satellite and sub-Satellite Data

  • Golik A.V.;Fischenko V.K.;Dubina V.A.;Mitnik L.M.
    • Proceedings of the KSRS Conference
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    • 2004.10a
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    • pp.371-374
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    • 2004
  • The task of development and usage in a corporate computer network of the Far Eastern Branch of the Russian Academy of Sciences (FEB RAS) of integrated technology of joint use by the scientists of satellite and sub satellite data on a Northwestern Pacific is considered. This integrated technology is realized by embedding of satellite data in the corporate oceanographic GIS of FEB RAS as a new information layer, and also by support of GIS by program techniques for specialized processing of both kinds of the data. As a result of integration the specialists of FEB RAS have an opportunity to carry out coordinated samples of satellite and various oceanographic data as a function of area, time and other important conditions, visualize them together and carry out analytical processing with the usage of the GIS tools. Application of the realized approach to improve the techniques of detection and description of the oceanic phenomena on ERS-l and ERS-2 SAR images as well as to improve of perspective techniques of the usage the brightness temperatures measured by a microwave radiometers AMSR-E on a board of Aqua (USA) satellites are discussed.

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GUI Implementation for 3D Visualization of Ocean Hydrodynamic Models (해수유동모델 결과의 3차원 가시화를 위한 GUI 구현)

  • Choi, Woo-Jeung;Park, Sung-Eun;Lee, Won-Chan;Koo, Jun-Ho;Suh, Young-Sang;Kim, Tae-Hyun
    • Journal of the Korean Association of Geographic Information Studies
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    • v.7 no.3
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    • pp.99-107
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    • 2004
  • This study presents an application of GIS technologies to construct the graphic user interface for 3-dimensional exhibition of the results obtained by ocean hydrodynamic model. In coastal management studies, GIS provide a receptacle for scattered data from diverse sources and an improvement of the 3D visualization of such data. Within the frame of a GIS a variety of analytical, statistical and modeling tools can be applied to transform data and make them suitable for a given application. A 3D hydrodynamic model was driven by time-dependent external forcing such as tide, wind velocity, temperature. salinity, river discharge, and solar radiation under the open boundary condition. The Jinhae bay was selected as a case study. Here, we have used GeoMania v2.5 GIS software and its 3D Analyst extension module to visualize hydrodynamic model result that were simulated around the Jinhae bay.

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Nonlinear analysis of reinforced concrete beams strengthened with polymer composites

  • Pendhari, S.S.;Kant, T.;Desai, Y.M.
    • Structural Engineering and Mechanics
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    • v.24 no.1
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    • pp.1-18
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    • 2006
  • Strengthening of existing old structures has traditionally been accomplished by using conventional materials and techniques, viz., externally bonded steel plates, steel or concrete jackets, etc. Alternatively, fibre reinforced polymer composite (FRPC) products started being used to overcome problems associated with conventional materials in the mid 1950s because of their favourable engineering properties. Effectiveness of FRPC materials has been demonstrated through extensive experimental research throughout the world in the last two decades. However there is a need to use refined analytical tools to simulate response of strengthened system. In this paper, an attempt has been made to develop a numerical model of strengthened reinforced concrete (RC) beams with FRPC laminates. Material models for RC beams strengthened with FRPC laminates are described and verified through a nonlinear finite element (FE) commercial code, with the help of available experimental data. Three dimensional (3D) FE analysis has been performed by assuming perfect bonding between concrete and FRPC laminate. A parametric study has also been performed to examine effects of various parameters like fibre type, stirrup's spacing, etc. on the strengthening system. Through numerical simulation, it has been shown that it is possible to predict accurately the flexural response of RC beams strengthened with FRPC laminates by selecting an appropriate material constitutive model. Comparisons are made between the available experimental results in literature and FE analysis results obtained by the present investigators using load-deflection and load-strain plots as well as ultimate load of the strengthened beams. Furthermore, evaluation of crack patterns from FE analysis and experimental failure modes are discussed at the end.

Feasibility Study of Mobius3D for Patient-Specific Quality Assurance in the Volumetric Modulated Arc Therapy

  • Lee, Chang Yeol;Kim, Woo Chul;Kim, Hun Jeong;Lee, Jeongshim;Huh, Hyun Do
    • Progress in Medical Physics
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    • v.30 no.4
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    • pp.120-127
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    • 2019
  • Purpose: This study was designed to evaluate the dosimetric performance of Mobius3D by comparison with an aSi-based electronic portal imaging device (EPID) and Octavius 4D, which are conventionally used for patient-specific prescription dose verification. Methods: The study was conducted using nine patients who were treated by volumetric modulated arc therapy. To evaluate the feasibility of Mobius3D for prescription dose verification, we compared the QA results of Mobius3D to an aSi-based EPID and the Octavius 4D dose verification methods. The first was the comparison of the Mobius3D verification phantom dose, and the second was to gamma index analysis. Results: The percentage differences between the calculated point dose and measurements from a PTW31010 ion chamber were 1.6%±1.3%, 2.0%±0.8%, and 1.2%±1.2%, using collapsed cone convolution, an analytical anisotropic algorithm, and the AcurosXB algorithm respectively. The average difference was found to be 1.6%±0.3%. Additionally, in the case of using the PTW31014 ion chamber, the corresponding results were 2.0%±1.4%, 2.4%±2.1%, and 1.6%±2.5%, showing an average agreement within 2.0%±0.3%. Considering all the criteria, the Mobius3D result showed that the percentage dose difference from the EPID was within 0.46%±0.34% on average, and the percentage dose difference from Octavius 4D was within 3.14%±2.85% on average. Conclusions: We conclude that Mobius3D can be used interchangeably with phantom-based dosimetry systems, which are commonly used as patient-specific prescription dose verification tools, especially under the conditions of 3%/3 mm and 95% pass rate.

Methods for quantitative measurement of tooth wear using the area and volume of virtual model cusps

  • Kim, Soo-Hyun;Park, Young-Seok;Kim, Min-Kyoung;Kim, Sulhee;Lee, Seung-Pyo
    • Journal of Periodontal and Implant Science
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    • v.48 no.2
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    • pp.124-134
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    • 2018
  • Purpose: Clinicians must examine tooth wear to make a proper diagnosis. However, qualitative methosds of measuring tooth wear have many disadvantages. Therefore, this study aimed to develop and evaluate quantitative parameters using the cusp area and volume of virtual dental models. Methods: The subjects of this study were the same virtual models that were used in our former study. The same age group classification and new tooth wear index (NTWI) scoring system were also reused. A virtual occlusal plane was generated with the highest cusp points and lowered vertically from 0.2 to 0.8 mm to create offset planes. The area and volume of each cusp was then measured and added together. In addition to the former analysis, the differential features of each cusp were analyzed. Results: The scores of the new parameters differentiated the age and NTWI groups better than those analyzed in the former study. The Spearman ${\rho}$ coefficients between the total area and the area of each cusp also showed higher scores at the levels of 0.6 mm (0.6A) and 0.8A. The mesiolingual cusp (MLC) showed a statistically significant difference (P<0.01) from the other cusps in the paired t-test. Additionally, the MLC exhibited the highest percentage of change at 0.6A in some age and NTWI groups. Regarding the age groups, the MLC showed the highest score in groups 1 and 2. For the NTWI groups, the MLC was not significantly different in groups 3 and 4. These results support the proposal that the lingual cusp exhibits rapid wear because it serves as a functional cusp. Conclusions: Although this study has limitations due to its cross-sectional nature, it suggests better quantitative parameters and analytical tools for the characteristics of cusp wear.

Validation of fetus aneuploidy in 221 Korean clinical samples using noninvasive chromosome examination: Clinical laboratory improvement amendments-certified noninvasive prenatal test

  • Kim, Min-Jeong;Kwon, Chang Hyuk;Kim, Dong-In;Im, Hee Su;Park, Sungil;Kim, Ji Ho;Bae, Jin-Sik;Lee, Myunghee;Lee, Min Seob
    • Journal of Genetic Medicine
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    • v.12 no.2
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    • pp.79-84
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    • 2015
  • Purpose: We developed and validated a fetal trisomy detection method for use as a noninvasive prenatal test (NIPT) including a Clinical Laboratory Improvement Amendments (CLIA)-certified bioinformatics pipeline on a cloud-based computing system using both Illumina and Life Technology sequencing platforms for 221 Korean clinical samples. We determined the necessary proportions of the fetal fraction in the cell-free DNA (cfDNA) sample for NIPT of trisomies 13, 18, and 21 through a limit of quantification (LOQ) test. Materials and Methods: Next-generation sequencing libraries from 221 clinical samples and three positive controls were generated using Illumina and Life Technology chemistries. Sequencing results were uploaded to a cloud and mapped on the human reference genome (GRCh37/hg19) using bioinformatics tools. Based on Z-scores calculated by normalization of the mapped read counts, final aneuploidy reports were automatically generated for fetal aneuploidy determination. Results: We identified in total 29 aneuploid samples, and additional analytical methods performed to confirm the results showed that one of these was a false-positive. The LOQ test showed that the proportion of fetal fraction in the cfDNA sample would affect the interpretation of the aneuploidy results. Conclusion: Noninvasive chromosome examination (NICE), a CLIA-certified NIPT with a cloud-based bioinformatics platform, showed unambiguous success in fetus aneuploidy detection.