• 대한한의학회지
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• 제39권1호
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• pp.35-43
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• 2018

Objectives: In the brain, glutamate is the most important excitable neurotransmitter in physiological and pathological conditions. However, the high level of glutamate induces neuronal cell death due to exitotoxicity and oxidative stress. The present study investigated to evaluate a possible neuroprotective effect of furosin isolated from Euphorbia helioscopia against glutamate-induced HT22 cell death. Methods: Furosin was isolated from methanol extract of Euphorbia helioscopia and examined whether it protects glutamate-induced neuronal cell death. The cell viability was determined using Ez-Cytox assay. Anti-oxidative effect of furosin was determined by DPPH scavenging activities, and the levels of intracellular reactive oxygen species (ROS) were determined by the fluorescent intensity after staining the cells with $H_2DCFDA$. To evaluate apoptotic cell death, we performed nuclear staining and image-based cytometeric analysis. Results: The cell viability was significantly increased by treatement with furosin compared with the treatment with glutamate. Furosin showed a strong DPPH radical scavenging activity ($EC50=1.83{\mu}M$) and prevented the accumulation of intra cellular ROS. Finally, the presence of 50 and $100{\mu}M$ furosin significantly the percentage of apoptotic cells compared with glutamate treatment. Conclusion: The present study found that furosin is a potent neuroprotectant against glutamate-induced oxidative stress through inhibition of apoptotic cell death induced by glutamate. Therefore, the present study suggests that furosin as a bioactive compound of E. helioscopia can be a useful source to develop a drug for the treatment of neurodegenerative diseases and acute brain injuries.

• 한국콘텐츠학회논문지
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• 제11권5호
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• pp.84-92
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• 2011

바이오 영상에서 세포 영역의 자동 분할 기술은 생물학자들이 복잡한 세포의 기능을 이해하는데 도움을 주고, 수작업을 통해 세포를 분석하던 일들을 자동적으로 처리해주는 매우 중요한 기술이다. 기존의 멀티채널 영상으로부터 세포핵 및 세포를 분할하는 방법은 DNA 채널을 이용하여 세포핵을 검출하고, 이를 초기 윤곽으로 하여 Actin 채널에서 밝기 기반의 Active Contour 모델을 통해 세포를 분할하는 2 단계의 과정을 거친다. 그러나 세포 분할 과정에서 채널 간 상관성으로 인해 발생하는 세포 내 불균일한 밝기 문제를 고려하지 않은 채, 밝기 기반의 Active Contour 모델을 적용하여 분할의 성능이 저하되는 문제점이 발생한다. 따라서 본 논문에서는 DNA 와 Actin 채널 간 상관성을 고려하여, DNA 채널 정보를 통해 Actin 채널 내부의 밝기를 균일하게 보정함으로써 밝기 기반의 Active Contour 모델이 세포 분할에 잘 적용 될 수 있는 전처리 알고리즘을 제안한다. 실험을 통해 제안 전처리 과정을 거친 세포 분할 방법의 성능이 기존 방법에 비해 객관적, 주관적으로 크게 향상됨을 증명한다.

• 한국콘텐츠학회논문지
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• 제7권1호
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• pp.40-47
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• 2007

단백질 매크로 어레이 영상에서 단백질 칩 각각의 특징을 규명하는 것은 중요한 것이다. 사람의 시각에 의한 판단은 많은 단백질 칩 영상을 실험할 경우, 장시간의 관찰과 그로 인한 오류가 발생할 수 있다. 따라서 시뮬레이터를 통한 특성 파악이 필요하게 되고, 매크로 어레이 스캔 영상에 대해 특성 분석을 할 경우 효율을 극대화할 수 있다. 형광 스캔 영상에 있어서, 각 셀의 반응도는 컬러 영상의 R, G, B 분포에 의존하여 왔다. 그러나 중첩되는 영상의 경우는 한쪽으로 구분하여 분류하기가 어렵다. 본 논문은 이러한 단점을 극복하기 위해 사용자가 원하는 색상에 대한 퍼지 측도 값을 적용한 퍼지 적분 값으로서 단백질 칩의 반응색상을 구분 지었다. Scan Array 5000에 의해 구성된 매크로 어레이 형광 영상들에 대해 실험한 결과, 퍼지 적분을 사용한 제안 방법이 모호한 색상에 대해 결정을 내릴 수 있는 요소가 됨을 보여 주었다.

• Environmental Analysis Health and Toxicology
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• 제23권1호
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• pp.47-51
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• 2008

The toxicity of environmental pollutants was measured between a image of the surface topography in HeLa cells using atomic force microscopy for the possibility of toxic effect measurement and environmental monitoring. A image of the surface topography by AFM were estimated as toxic endpoints. The surface topography by AFM was observed a change of the cell surface in the environmental pollutants, but the standard of the measurement requires for the dose-effect degree. The overall results indicate that the possibility of measurement using AFM were confirmed a dose-effect degree related toxic effects, but it requres correlation between more various biomarker and AFM's measurements if the possibility of the toxic effect measurement was established.

• 동의신경정신과학회지
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• 제19권3호
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• pp.117-127
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• 2008

Objective: Bee venom (BV) has been used for the treatment of inflammatory diseases such as rheumatoid arthritis and relief of pain in Oriental medicine. The two main components of BV are melittin and phospholipase A2 (PLA2). Of these, melittin, the major active ingredient of BV, has been reported to induce apoptosis and to possess anti tumor effects. Several studies have established that the agents inducing apoptosis in target organs suppress tumorigenesis. As the other component, PLA2 has been reported to induce neurite outgrowth in PC12 cells. However, there was no report about proliferative effect of BV in neuronal cells. In order to examine the effect of BV on glioma cell, human glioma cell line, U87 was used. Methods: Analysis of proliferation was confirmed by MTT assay. BV increased cell number through dose and duration dependent manner and these effects are apparent at a concentration of 10 ug/ml. To observe which signaling molecules will be activated by BV, phosphorylation of Akt, MAPK, PYK2 or CREB were examined by Western blot analysis. To study the long term effect of BV in U87 cells, the image of cells treated with BV for 4 days were obtained. Results: The phosphorylation levels of PYK2 and Akt were increased at 5 min after addition of 10 ug/ml of BV and sustained to 2 hours. On the other hand, phosphorylation of MAPK and CREB were increased at 5 min, maximum at 10 min, and returned to 30 min. These imply that BV may activate two different signaling pathways, PYK2/Akt and MAPK/CREB. BV treated cells showed increased neurite number and length. Conclusion: These results propose that BV may induce differentiation as well as proliferation of U87 cells through the activation of PYK2/ Akt and MAPK/ CREB.

• 한국가시화정보학회지
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• 제9권2호
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• pp.16-20
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• 2011

This paper presents a fabrication method of microcapsules encapsulating fluorescent nanoparticles sensitive to an organic liquid, which is potentially applicable to the encapsulation of protein, cell and drug. It uses the supra-molecular self-assembly of a block copolymer at the interface of the stable and controllable droplets of water suspended with fluorescent nanoparticles and the polymer solved organic. The size and uniformity of the microcapsules were examined for the various polymer concentrations by using SEM image analysis. The maximum standard deviation of the produced microcapsules of less than 3.5% was obtained from the microcapsules produced from the same conditions. The inclusion of fluorescent nanoparticles was visualized in the fluorescence microscope and by using TEM image. It is shown that this fabrication method can provide the uniform size microcapsules with a higher inclusion.

• 동력기계공학회지
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• 제4권2호
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• pp.11-16
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• 2000

Simultaneous quantitative measurements are made of the velocity fields for two-dimensional natural convection in a rectangular enclosure using PIV(Particle Image Velocimetry). The experiments are performed at a Prandtl number of 6.62, an aspect ratio of 1.0, Rayleigh numbers from $1.294{\times}10^6\;to\;3.8841{\times}10^6$, and angles of inclination of $0^{\circ},\;30^{\circ}\;and\;60^{\circ}$ inside a $30mm{\times}30mm{\times}8mm$ cavity made of an acrylic glass 10mm, with two isothermal copper walls kept at a prescribed temperature. The experimental results agreed very well with the numerical results. It was found that the flow consisted of a large double convection cell at angle of inclination of $60^{\circ}$.

• 한국작물학회지
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• 제49권1호
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• pp.36-40
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• 2004

To observe and analysis ultra-microscopically barley aleurone cell surface, atomic force microscope (AFM) was used. Seed coat of early maturing germplasm, eam9, was dehulled and scanned by non-contact mode. We have obtained the high resolution topographic 3-dimensional image of barley aleurone layer with high resolution. These images showed the membrane proteins in barley aleurone cell. One channel protein and numerous peripheral or integral proteins were detected in a area of 100 $\mu\textrm{m}^2$. Furthermore, we found that their widths were ranged from 50 to 750nm and lengths from 0 to 66 $\mu\textrm{m}$. The thickness of aleurone layer was measured by scanning electron microscope. The thickness at early developmental stage was about 16 and then the aleurone cell enlarged upto 57 $\mu\textrm{m}$${\mu}{\textrm}{m}$ at least until 42 days after anthesis. In this study, we firstly reported on the ultrastructural AFM analysis of living aleurone cell as a biological specimen. It was clearly suggested that AFM will become an powerful tool for probing both the structural properties of biological samples.

• 한국식품영양학회지
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• 제6권3호
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• pp.178-188
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• 1993

This research was attempted to investigate changes in physicochemical properties of rice extrudate with added ginger powder extruded by single screw extruder. Graphic three dimensional analysis on response surface regression was used to evaluate effects of extrusion variables on quality factors of the extrudate according to two independent variables, ginger consent 0∼12%, moisture content 14∼26%. The summarized results are as follows : 1) Regarding proximate composition of rice extrudate with added ginger powder, as ginger powder content of raw material Increased, crude tat, crude protein, crude ash and crude fiber increased, while soluble nitrogen free extract decreased. 2) Graphic three dimensional analysis on response surface regression was conducted for each dependent variable which revealed statistically significant relationship with independent variables, 0∼120A ginger and 14∼26% moisture content. Expansion ratio had a critical point as moisture content changed. As ginger and moisture content Increased, bulk density, break strength and water absorption Index Increased, while water solubility Index decreased. The predicted maximum degree of gelatinization in 6.15% ginger and 15.56% moisture content is 88.27%, and lightness decreased as ginger content Increased. According to the microstructure for the cross section of extrudate obsorbed with image analyzer, air cell number and perimeter revealed saddle point, meanwhile total area and fractarea of air cell had critical points as moisture content changed. In view of the results, quality of rice extrudate with added ginger powder was optimum when rice flour was fed to the extruder with 2∼7% singer powder and 15∼20% moisture content.

• 통상정보연구
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• 제18권3호
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• pp.101-130
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• 2016

최근 소비시장으로서 중국의 중요성이 증대됨에 따라 중국 소비자들에 대한 체계적이고 과학적인 연구의 필요성이 제기되고 있다. 이러한 맥락에서 본 연구는 국가이미지와 기능적 속성 및 브랜드개성이 중국 소비자들의 한국제품 선택에 어떠한 영향을 미치는가를 성(性)별 비교분석을 통해 살펴보고 있다. 이를 위하여 중국 북경(北京), 상해(上海), 광주(廣州) 지역에 거주하는 성인 소비자들을 대상으로 한국산 핸드폰 제품에 관한 설문조사를 실시하였으며, 실증분석 결과 나타난 주요 연구결과를 요약하면 다음과 같다. 첫째, 여성 소비자들이 한국 핸드폰 선택시 남성 소비자들에 비해 국가이미지와 브랜드개성의 영향을 상대적으로 더 많이 받는 것으로 나타났다. 둘째, 남성과 여성 소비자 모두 한국 핸드폰 선택시 다른 두 요인에 비해 기능적 속성 요인의 영향을 더 많이 받는 것으로 나타났다. 본 연구에서는 이러한 연구결과를 토대로 한국기업들의 바람직한 대중국 마케팅 전략에 관한 주요 시사점을 제시하고 있다.