• Title/Summary/Keyword: Anaerobic condition

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Assessment of Pollution Characteristics of Surface Sediments from Lake Andong(II): Studies on the Nutrient and Heavy Metal Release Characteristics from Sediments in Andong Dam (안동댐 퇴적물의 오염도 평가(II): 안동댐 퇴적물에 대한 영양염류 및 중금속 용출 특성 연구)

  • Kim, Young Hun;Park, Jae Chung;Shin, Tae Cheon;Kim, Jeong Jin
    • Korean Journal of Mineralogy and Petrology
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    • v.33 no.4
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    • pp.391-405
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    • 2020
  • Leaching chracteristics of Andong-dam sediment was conducted for heavy metal and nutrients. Five mixed sediment samples were prepared and leaching was conducted under aerobic and anaerobic condition for 60 days. Cd, Cu, Pb, Cr, Zn, Hg, As, Fe, Mn, phosphorus, and nitrogen were analyzed at each sampling time. The leaching rate of phosphorus was higher in anaerobic condition comparing with that of under aerobic condition. Some samples showed higher than the water-quality level IV. In case of As and Cd which showed highest contamination level in the sediment, leached concentration were 0.028 mg/L and 0.003 mg/L in maximum, respectively. The leached concentration is below than the lake water quality standard of Korea. Other heavy metals including Cu, Pb, and Cr also showed similar trend. Five step sequential extraction showed that easily extractable 1-2 step portion such as ion-exchangeable and adsorbed one was less than 10% and the most of the portion was residual. For As and Cd, the residual portion were 80% and 95% respectively indicating the risk by the heavy metal leaching into the lake for a short period was not high in comparing with the contamination levels.

Isolation and Characterization of Phosphorus Accumulating Acinetobacter CW3 (인 축적균 Acinetobacter CW3의 분리 및 특성)

  • 심성훈;류원률;이영호;김정목;조무환
    • KSBB Journal
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    • v.14 no.1
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    • pp.71-75
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    • 1999
  • A highly effective phosphorus accumulating bacterium named Acinetobacter CW3 was isolated from the nature by using Winogradsky columns. The optimal cultivation conditions of Acinetobacter CW3 in shaking flask were determined as $20^{\circ}C$, pH 7, 200rpm, 18.5mg $PO_4$-P/L. Acientobacter CW3 could remove phosphorus completely in 12hours for a batch culture at optimal cultivation condition. This bacterium could uptake phosphorus on aerobic condition and release it on anaerobic condition.

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Effects of Water Circulation on the Phosphorus Release Rate from Sediments in the Lake (호수의 물 순환이 저니의 인 용출율에 미치는 영향)

  • Kim, Geonha;Jeong, Woohyeok;Choi, Seunghee
    • Journal of Korean Society on Water Environment
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    • v.21 no.6
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    • pp.595-601
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    • 2005
  • In this research, effects of water circulation on phosphorus release from sediment into water body were studied. Sediments sampled at the Daechung Lake were used for the column experiments with circulation and non-circulation conditions. Deaeration coefficient, $K_1$ and reaeration coefficient, $K_2$ of non-circulation condition were 0.133 and 0, respectively, while $K_1$ and $K_2$ for circulation condition were 0.46 and 0.018, respectively. Oxidation Reduction Potential (ORP) showed a linear relationship with dissolved oxygen (DO) when DO is over 2 mg/L. Phosphorus concentration induced by phosphorus release from sediment was highly dependent upon DO, ORP, and pH. Under anaerobic condition, phosphorus release rate was higher for $Fe^{2+}$-bounded phosphorus compared to that of $Ca^{2+}$-bounded phosphorus.

Influence of Gluconeogenic Phosphoenolpyruvate Carboxykinase (PCK) Expression on Succinic Acid Fermentation in Escherichia coli Under High Bicarbonate Condition

  • Kwon Yeong-Deok;Lee Sang-Yup;Kim Pil
    • Journal of Microbiology and Biotechnology
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    • v.16 no.9
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    • pp.1448-1452
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    • 2006
  • The effects of amplifying the gluconeogenic phosphoenolpyruvate carboxykinase of Escherichia coli ($pck_{Ec}$) on succinic acid production in E. coli were examined under anaerobic condition. No significant increase in succinic acid production was observed in E. coli overexpressing the $pck_{Ec}$ gene without supplementing $NaHCO_{3}$ or $MgCO_{3}$. On the other hand, succinic acid production was enhanced as the $NaHCO_{3}$ concentration was increased. When 20 g/l of $NaHCO_{3}$ was added, succinic acid production in recombinant E. coli overexpressing PCK was 2.2-fold higher than that observed in the wild-type strain. It was concluded that the gluconeogenic $pck_{Ec}$ overexpression enabled E. coli to enhance succinic acid production only under the high bicarbonate supplementation condition.

Design of convection current circulation system in reservoir using CFD simulation (CFD모사를 이용한 저수지 물순환장치 유동 설계)

  • Lee, Yosang
    • Journal of Environmental Impact Assessment
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    • v.21 no.1
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    • pp.133-142
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    • 2012
  • Convection Current Circulation System(CCCS) in stratified reservoir controls development of anaerobic condition and algal bloom during summer. In order to increase the CCCS effectiveness, we analyze diverse design parameters to make optimize the flow pattern in reservoir. In this study, we interpret the internal flow with installation and operation condition of CCCS based on CFD in reservoir. Design variables of CCCS is reservoir depth, stratification strength, distance of between CCCS and so on. Since reservoir depth and stratification strength in variables is depending on natural phenomenon, we evaluated current circulation effect by distance of CCCS and proposed the optimal design condition using CFD simulation. Flow and diffusion changes in water body was assessed by temperature and dye test. Changes in water floor temperature at 40m intervals was slowly descending over 37 hours. Dye diffusion simulation at 60m intervals, the radius of the spread between two devices were overlapped after 12 hours.

Repeated Fed-Batch Fermentation of Wheat Flour Solution by Mixed Lactic Acid Bacteria (혼합 젖산균을 이용한 밀가루 용액의 반복 유가식 발효)

  • Kim, Sang-Yong;Noh, Bong-Soo;Oh, Deok-Kun
    • Korean Journal of Food Science and Technology
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    • v.29 no.2
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    • pp.343-347
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    • 1997
  • Effect of culture conditions on the fermentation of wheat flour solution by mixed lactic acid bacteria of Lactobacillus brevis, L. fermentum and L. plantarum was investigated. The optimum temperature for the fermentation of wheat flour solution was $35^{\circ}C$ because pH decreased the lowest value and TTA (total titrable acidity) increased the highest value at this temperature. In aerobic condition, fermentor was purged with air at 1.0 vvm and was purged with nitrogen gas at 1.0 vvm in anaerobic condition. The decrease of pH and the increase of TTA in aerobic condition were higher than those in anaerobic condition. In aerobic condition, the optimum condition of oxygen supply was found to be oxygen transfer rate coefficient of $60\;hr^{-1}$ which corresponded to agitation speed of 250 rpm in a 5 L fermentor. Repeated fed-batch cultures were performed using pH-stat in order to increase the productivity of fermented wheat flour. With increasing the repeated fraction of culture volume, mean cycle time increased but maximum operation time decreased. However, the volume of produced broth per culture volume per time and total volume of produced broth per culture volume were maximum at the repeated fraction of culture volume of 20%. In a repeated fed-batch fermentation of wheat flour solution using mixed lactic acid bacteria, the culture condition was optimum at temerature of $35^{\circ}C$, aeration rate of 1.0 vvm, oxygen transfer rate coefficient of $60\;hr^{-1}$, and repeated fraction of culture volume of 20%.

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Effects of Global Warming on the Estuarine Wetland Biogeochemistry (기후변화가 하구 습지 토양의 생지화학적 반응에 미치는 영향에 관한 연구)

  • Ki, Bo-Min;Choi, Jung-Hyun
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.8
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    • pp.553-563
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    • 2011
  • This study investigated the effects of elevated $CO_2$ and nitrogen addition on the anaerobic decomposition mediated by microorganisms to determine the microbial metabolic pathways in the degradation of organic matters of the sediments. There were statistically significant differences(P < 0.05) in the rates between denitrification and methanogenesis upon increased $CO_2$ concentration, nitrogen addition, in the presence of plants. Based on the assumption that anaerobic degradation of organic matter mainly occurs through denitrification, iron reduction, and methanogenesis, methanogenesis is the dominant pathways in the decomposition of organic matter under the condition of elevated $CO_2$ and nitrogen addition. In addition, the altered environment increased anaerobic carbon decomposition. Therefore, it can be concluded that freshwater wetland sediments have positive effects on the global warming by the increased methanogenesiss as well as increased anaerobic carbon decomposition.

Removal of Organic Matter, Nitrogen and Phosphorus in an UASB Sewage Treatment Process with Recycle of Aerated Bio-Filter Effluent (UASB를 이용한 혐기성 하수처리공정에서 ABF 유출수 반송에 따른 유기물질과 질소 및 인 제거)

  • Tian, Dong-Jie;Byun, Byeong-Su;Lim, Hyun-Suk;Jun, Hang-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.5
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    • pp.381-387
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    • 2013
  • Nitrogen and phosphorus removal was investigated in an UASB-ABF (Up-flow anaerobic sludge blanket - aerated bio-filter) anaerobic sewage treatment system. Successful removal of nitrogen and phosphorus with organic matters was possible in the UASB-ABF system from the results of 160 days operation with the influent raw domestic sewage. Removal efficiencies of organic matter (as TCOD) showed 64% in UASB without recycle of the ABF effluent, however, they increased to 92%, 95%, 96% with 120%, 180% and 240% recycle of the ABF effluent, respectively. Increasement of the organic matter removal was not prominent at recycle ratio above 180%. Apparent increase in TN removal occurred with recycle of the ABF effluent. TN removal efficiency was 18% without recycle, but it increased to 82% with 240% recycle of the ABF effluent. And stable nitrification above 95% was possible as a result of efficient removal of organic matter in the UASB with and without recycle of the effluent. Removal of both TP and $PO{_4}^{3-}$-P also increased remarkably with recycle of the effluent. Without recycle of the effluent, that is at strict anaerobic condition in UASB, TP was not removed, however, its removal efficiency increased to 51%, 63%, 71% at recycle ratios of 120%, 180%, 240%, respectively mainly at UASB.

A Proposal of Sequencing the Combined Processes for Resources Recovery and Nitrogen Removal from Piggery Waste (슬러리형 돈사폐수에서 자원회수와 질소제거를 위한 순차적 결합공정 제안)

  • Hwang, In-Su;Min, Kyung-Sok;Bae, Jin-Yeon
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.1
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    • pp.61-66
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    • 2006
  • The combined ADEPT(Anaerobic Digestion Elutriated Phased Treatment)-SHARON(Single reactor system for High Ammonium Removal Over nitrite)-ANAMMOX(Anaerobic ammonium oxidation) processes were operated for resources recovery and nitrogen removal from slurry-type piggery waste. The ADEPT process operated at an acidogenic loading rates of 3.95 gSCOD/L-day, the SCOD elutriation rate and acid production rate were 5.3 gSCOD/L-day and 3.3 gVFAs(as COD)/L-day, respectively. VS reduction and SCOD reduction by the hydrolysis were 13% and 0.19 $gSCOD_{prod.}/gVS_{feeding}$, respcetively. Also, the acid production rate was 0.80 $gVFAs/gSCOD_{prod}$. In methanogenic reactor, the gas production rate and methane content were 2.8 L/day($0.3m^3CH_4/kgCOD_{removal}@STP$) and 77%, respectively. With these operating condition, the removals of nitrogen and phosphorus were 94.1% as $NH_4-N$(86.5% as TKN) and 87.3% as T-P respectively.

A Study of Milk Waste Recycling as an Energy Source and Reduction of Pollution by Anaerobic Digestion (혐기성 소화를 통한 유가공 폐기물의 에너지원으로의 재활용과 오염 감소 방안에 관한 연구)

  • Lim, Samuel;Lim, Hyun-Ji;Jung, Kook-Jin
    • Journal of Dairy Science and Biotechnology
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    • v.27 no.1
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    • pp.13-18
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    • 2009
  • We confirmed methane production and reduction of pollution during anaerobic digestion of milk waste and analyzed the economic potential of using milk waste as a renewable energy source. The milk waste sludge was obtained from the Pasteur milk factory and processed by anaerobic digestion to produce methane. The methane production from two completely mixed tank reactors with an effective capacity of 6 ${\ell}$, 15 days of hydraulic retention time (HRT), and a mid-temperature of $35^{\circ}C$ averaged 4.11 ${\ell}$/day. The total chemical oxygen demand (TCOD) during production decreased from an initial 31,416 mg/${\ell}$ to 13,500 mg/${\ell}$, showing a maximum TCOD removal efficiency of 60%. When HRT was reduced to 12 days, methane production increased by 44% under a high-temperature condition of $55^{\circ}C$. An economic analysis based on these results was applied to a Korean milk factory of typical size and demonstrated that the installation of an anaerobic digester could provide sufficient economic profit.

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