• Journal of Soil and Groundwater Environment
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• v.8 no.4
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• pp.27-35
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• 2003

The study site located in an industrial complex has a Precambrian age gneiss as a bedrock. The poorly-developed, disturbed soils in the study site have loamy-textured surface soil (1 to 2 m) and gravelly sand alluvium subsurface (2 to 6 m) on the top of weathered gneiss bedrock. The depth of the groundwater table was about 3.5 m below ground surface and increased toward down-gradient of the site. The hydraulic conductivity of transmitted zone (gravelly coarse sand) was in the range of 5.0${\times}$10$\^$-2/∼1.85${\times}$10$\^$-1/ cm/sec. The fine sand layer was in the range of 1.5${\times}$10$\^$-3/ to 7.6${\times}$10$\^$-3/ cm/sec. and the reclaimed upper soil layer was less than 10$\^$-4/ cm/sec. Toluene, ethylbenzene, and xylene (TEX) was the major contaminant in the soil and groundwater. The average depth of the soil contamination was about 1.5 m in the gravelly sand alluvium layer. At the depth interval 2.4∼4.8 m, the highest contamination in the soil is located approximately 50 to 70 m from the suspected source areas. The concentration of TEX in the groundwater was highest in the suspected source area and a lesser concentration in the center and southwest parts of the site. The TEX distribution in the groundwater is associated with their distribution in the soil. Microbial isolation showed that Pseudomonas flurescence, Burkholderia cepacia, and Acinetobactor lwoffi were the dominant aerobic bacteria in the contaminated soils. The analytical results of the groundwater indicated that the concentrations of dissolved oxygen (DO), nitrate, and sulfate in the contaminated area were significantly lower than their concentrations in the none-contaminated control area. The results also indicated that groundwater at the contaminated area is under anaerobic condition and sulfate reduction is the predominant terminal electron accepting process. The total attenuation rate was 0.0017 day$\^$-1/ and the estimated first-order degradation rate constant (λ) was 0.0008 day$\^$-1/.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.231-237
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• 1999

It is very distinctive that sigumjang meju was produced with smoking after molding. Every shapes of traditional sigumjang meju gathered from Kyeongsangbukdo were doughnut types. Fermentation time were $60{\sim}90$ days. Aerobic bacteria were $6.8{\times}10^7\;cfu/g$, and anaerobic bacteria, yeasts, molds count $3.2{\times}10^6\;cfu/g,\;1.0{\times}10^6\;cfu/g\;and\;4.0{\times}10^6\;cfu/g$, respectively. Analysis showed: moisture content, $10.5{\pm}2.6%$; fat content, $2.9{\pm}1.1%$; protein content, $8.3{\pm}0.7%$; ash content, $3.8{\pm}0.7%$; pH, $6.0{\pm}0.5$; L-value, $54.5{\pm}4.7$; a-value, $+3.3{\pm}0.7$; and b-value, $+10.7{\pm}2.0$. K content was the most in quantity among the minerals in sigumjang meju. Maltose was most abundant component among the tested sugars followed by mannitol. Total content of amino acid was $1524.9{\pm}1295.3\;mg%$. Proline was the most abundant component among the amino acids. The ratio of essential amino acid was $30.0{\pm}12.9%$. The principal fatty acids were 18:2, 18:1 and 16:0. Saturated fatty acid and mono unsaturated fatty acid were $23.2{\pm}1.9%\;and\;23.5{\pm}5.2%$, respectively. The PUFA/SFA was $2.3{\pm,}0.3$ which higher than 1.

• Journal of the Mineralogical Society of Korea
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• v.21 no.2
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• pp.139-159
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• 2008

Geochemical analysis of the various kinds of water including observation borehole groundwater was carried out for the assessment of the hydrological safety of the underground oil storage cavern and the potentiality of mineralogical and microbiological clogging was estimated. Most of water samples belonged to $Ca-HCO_3$ and $Ca-HCO_3-SO_4$ types. There was no distinct chemical difference in the various kinds of water. All kinds of water are undersaturated with the calcite which is the major clogging mineral. Most water samples have low Fe and Mn concentrations. However, they are saturated or oversaturated with the iron-oxide/hydroxide minerals and have high dissolved oxygen contents which suggests the possibility of clogging by the iron-oxide/hydroxide minerals as a long-term aspect. Several water samples from the ground observation borehole also show the high saturation indices far the clay minerals, which can fill up the fractures, indicating the possibility of clogging by the clay minerals. Statistical analysis shows the degree of mineral precipitation or dissolution is mainly controlled by pH, Eh and DO of water samples. According to the microbial analysis, the aerobic microbes and slime forming bacteria are dominant in most water samples and anaerobic microbes including sulfate reducing bacteria are very low or not detected. Although the slime forming bacteria which are known as a main microbial cause of the clogging is lower than $10^5\;CFUs/mL$ in all water samples, because the slime forming bacteria are dominant microbe in several observation boreholes, the clogging can be caused by it as a long-term aspect. In addition, the possibility of clogging can be increased if the microbial effect is combined with the mineralogical effect such as iron oxide/hydroxide minerals for the possibility of clogging. Therefore, the systematic and long-term program for the assessment of clogging is required for the safe operation of underground oil storage cavern.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.2
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• pp.41-48
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• 2017

A vertical multi-stage ammonia stripping reactor using E-PFR, which has been proved to be superior in anaerobic and aerobic treatment, was developed and a lab scale experiment was conducted. According to the change of stage number condition, the removal rate of the ammonia nitrogen in the reactor with 0-stage was about 52.5% after 8 hours (pH 10, temperature $35^{\circ}C$, and the air/liquid ratio $3min^{-1}$) However, in the reactor with 5-stage, the removal efficiency was about 62.6%. According to the change of pH condition, the removal rate of ammonia nitrogen was about 42.6% at pH 9 after 8 hours, and was about 74.4% at pH 11 (5-stage reactor, temperature $35^{\circ}C$, and the air/liquid ratio $3min^{-1}$). According to the change of temperature condition, the removal rate of the ammonia nitrogen was about 51% at $25^{\circ}C$ after 8 hours (5-stage reactor, pH 10, and the air/liquid ratio $3min^{-1}$), and was about 87.2% at $45^{\circ}C$. According to the change of air injection volume condition, the removal rate of the ammonia nitrogen was about 45.8% at $2min^{-1}$ after 8 hours (5-stage reactor, pH 10, and at $35^{\circ}C$). and was about 75% at $4min^{-1}$. Based on these results, we will follow up the applicability of the actual plant in the future through continuous operation evaluation.

• Journal of Life Science
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• v.17 no.12
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• pp.1660-1668
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• 2007

The non-ionic surfactant (NIS) Tween 80 was evaluated for its ability to influence invitro cumulative gas production, dry matter digestibility, cellulolytic enzyme activities, anaerobic microbial growth rates, and adhesion to substrates by mixed rumen microorganisms on rice straw, alfalfa hay, cellulose filter paper and tall fescue hay. The addition of NIS Tween 80 at a level of 0.05% increased significantly (P<0.05) in vitro DM digestibility, cumulative gas production, microbial growth rate and cellulolytic enzyme activity from all of substrates used in this study. In vitro cumulative gas production from the NIS-treated substrates; rice straw, alfalfa hay, filter paper and tall fescue hay was significantly (P<0.05) improved by 274.8, 235.2, 231.1 and 719.5% compared with the control, when substrates were incubated for 48 hr in vitro. The addition of 0.05% NIS Tween 80 to cultures growing on alfalfa hay resulted in a significant increase in CMCase (38.1%), xylanase (121.4%), Avicelase (not changed) and amylase (38.2%) activities after 36 h incubation. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some kinds of cellulolytic enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our SEM observation showed that NIS Tween. 80 did not influence the microbial adhesion to substrates used in the study. Present data clearly show that improved gas production, DM digestibility and cellulolytic enzyme activity by Tween 80 is not due to increased bacterial adhesion on the substrates.

• Journal of the korean academy of Pediatric Dentistry
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• v.27 no.2
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• pp.185-191
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• 2000

The water-soluble glucan produced by Lactococcus lactis 1370 affects the formation of dental plaque by Streptococcus mutans. In this study the factors affecting the formation of water-soluble glucan were assessed as the optical density of culture supernatant of Lactococcus lactis 1370 in the spectrophotometer. 1. The optical density of culture supernatant was high when Lactococcus lactis 1370 was cultured in M17 broth with 5% sucrose, while being low in culture supernatant of Streptococcus mutans. 2. The optical density of culture supernatant was higher when Lactococcus lactis 1370 was cultured in M17 broth with 10% sucrose than when being cultured without sucrose (p<0.05), and was higher at pH 7 than pH 5 (p<0.05). 3. The optical density of culture supernatant was the highest at $37^{\circ}C$ among $32^{\circ}C,\;37^{\circ}C\;and\;42^{\circ}C$, and was higher in the anaerobic incubator than in the aerobic incubator (p<0.05). 4. The optical density of culture supernatant was the highest in the media containing 1.0mM $CaCl_2$ (p<0.05), 2.5mM KCl (p<0.05), and 1.6mM $MgCl_2$. 5. When Streptococcus mutans was cultured in the media containing a quarter culture supernatant of Lactococcus lactis 1370 grown in M17 broth, the mean weight of produced artificial plaque was 103.0mg on the wire, whereas being significantly reduced to 5.6mg in the media containing a quarter culture supernatant of Lactococcus lactis 1370 grown in M17 broth containing 5% sucrose (p<0.05). These results indicate that the water-soluble glucan is more formed by Lactococcus lactis 1370 in the media containing sucrose or under the adequate conditions for the growth of bacteria, and inhibits the formation of artificial plaque by Streptococcus mutans.

• Journal of Life Science
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• v.20 no.2
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• pp.260-268
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• 2010

The lactate dehydrogenase (EC 1.1.1.27, LDH) isozymes in tissues from Channa argus were purified and characterized by biochemical, immunochemical and kinetic methods. The activity of LDH in skeletal muscle was the highest at 380.4 units and those in heart, eye and brain tissues were 13.4, 3,5 and 5.4 units, respectively. Citrate synthase (EC 4.1.3.7, CS) activity in heart tissue was the highest at 20.7 units. LDH/CS in skeletal muscle, heart, eye and brain tissues were 172.9, 0.6, 0.32 and 0.47. Protein concentration in skeletal muscle tissue was 14.7 mg/g and specific activities of LDH in skeletal muscle, heart, eye and brain tissues were 25.88, 0.79, 0.31 and 1.38 units/mg, respectively. Therefore, skeletal muscle tissue was anaerobic and heart tissue was aerobic. The LDH isozymes in tissues were identified by polyacrylamide gel electrophoresis, immunoprecipitation and Western blot with antiserum against $A_4$, $B_4$, and eye-specific $C_4$. LDH $A_4$, $A_3B$, $A_2B_2$. $AB_3$ and $B_4$ isozymes were detected in every tissue, $C_4$, $AC_3$, $A_2C_2$ and $A_3C$ were detected in eye tissue, and $A_3C$ was found in brain tissue. LDH $A_4$, $A_3B$, $A_2B_2$, $AB_3$, $B_4$, eye-specific $C_4$ isozymes were purified by affinity chromatography and Preparative PAGE Cells. The LDH $A_4$ isozyme was purified in the fraction from elution with $NAD^+$ containing buffer of affinity chromatography. Eye-specific $C_4$ isozyme was eluted right after $A_4$, after which $B_4$ isozyme was eluted with plain buffer. As a result, one part of molecular structures in $A_4$, $B_4$ and eye-specific $C_4$ were similar, but were different from each other in $B_4$ and $C_4$. Therefore the subunit A may be conservative in evolution, and the evolution of subunit B seems to be faster than that of subunit A. The activity of LDH $A_4$, $A_2B_2$, $B_4$, and eye-specific $C_4$ isozymes remained at 39.98, 21.28, 19.67 and 16.87% as a result of the inhibition by 10 mM of pyruvate, so the degree of inhibition was very high. The $Km^{PYR}$ values were 0.17, 0.27 and 0.133 mM in $A_4$, $B_4$ and eye-specific $C_4$ isozymes, respectively. The optimum pH of LDH $A_4$, $B_4$, eye-specific $C_4$, $A_2B_2$, $A_3B$, and $AB_3$ were pH 6.5, pH 8.5, pH 5.5, pH 6.0-6.5, pH 5.0 and pH 7.5. The $A_4$ and heterotetramer isozymes stabilized a broad range of pH. Especially, LDH activities in skeletal muscle tissue were high, resulting in a high degree of muscle activity.LDH metabolism in eye tissue seems to be converted faster from pyruvate to lactate by eye-specific $C_4$ isozyme as eye-specific $C_4$ have the highest affinity for pyruvate, and right after the conversion, oxidation of lactate was induced by $A_4$ isozyme. It was found that expression of Ldh-C, affinity to substrate and reaction time of $C_4$ isozyme were different according to the ecological environmental and feeding capturing patterns.

• Applied Biological Chemistry
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• v.19 no.2
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• pp.82-92
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• 1976

The study was carried out to investigate the changes of the various chemical components and the microflora during the aging period of Korean navive Kochuzang. (Red pepper soybean paste) Korean native maeju loaves were separated into surface and inner parts. Three kinds of Korean native Kochuzang were prepared from surface part, inner part, and ordinary of maeju. The selection and the indentification of the high enzyme producing strains from the microflora and characteristics of their enzymes were studied. I. The changes of the various chemical components during the aging period of Kochuzang. 1) The changes of pH in the 3 kinds of Kochuzang displayed rapid decrease for the first 10 days after preparing and gradual curve of decrease until 60 days, but slight increase for the next 30 days. The pH of the surface part Kochuzang was lower than that of inner part or ordinary Kochuzang. 2) The total acid contents in the 3 kinds of Kochuzang showed gradual increase until the 60 days but it slowly reduced after this time. 3) The total nitrogen contents in the 3 kind of Kochuzang showed gradual inerease up to the 60 days, but slight decrease after this time. 4) The changes of trichloroacetic acid soluble nitrogen in the 3 kinds of Kochuzang showed a remarkable increase for the first 10 days, however gradual increase after this time. 5) The increase of amino nitrogen contents in the 3 kinds of Kochuzang seemed to be remarkable until the first 30 days, however to be less remarkable after this time. 6) The contents of reducing sugar in the 3 kinds of Kochuzang showed remarkable increase until the first 50 days and it slowly reduced after this time. II. The changes of microflora during the aging period of Kochuzang. 1) Aerobic, anaerobic bacteria and mold in the 3 kinds of Kochuzang were increased until the first 30 to 40 days, but they were reduced after this time. 2) No yeast in the three kinds of Kochuzang appeared until the first 20 days. Yeast were proved to grow, when the pH value was decreased below 5.4 after the 30 days. Yeasts in the surface part and ordinary Kochuzang were gradually increased and those in the inner part Kochuzang were decreased as aging. III. The selection and identification of high amylase and protease producing strains from the microflora during the aging period of Kochuzang. 1) The amylase and protease highly producing strains from microflora were identified as Bacillus subtilis-P, Bacillus subtilis-G, Bacillus licheniformis-K, Aspergillus oryzae-B. 2) Amylase activity of Aspergillus oryzae-B was highest among the strains and the strains in order of the higher activity to the lower one were Bacillus subtilis-P Bacillus licheniformis-K, Bacillus subtilis-G. Protease activities of Aspergillus oryzae-B and Bacillus subtilis-P were about the same and the strains in order of the higher activity to the lower one were Bacillus licheniformis-K, Bacillus subtilis-G. 3) Amylase activity was inhibited more than protease activity was with NaCl concentration. Amylase activity was inhibited by 45 to 65 percent and protease activity by 40 to 46 percent at the concentration of 15 percent NaCl, which was the average concentration of NaCl in Kochuzang.