• The Journal of Korean Medicine
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• v.30 no.1
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• pp.51-63
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• 2009

Objectives: Peroxynitrite ($ONOO^-$), superoxide anion radical (${\cdot}{O_2}^-$ and nitric oxide (NO) are cytotoxic because they can oxidize several cellular components such as proteins, lipids and DNA. They have been implicated in the aging processes, and age-related diseases such as Alzheimer's disease, rheumatoid arthritis, cancer, diabetes, obesity and atherosclerosis. The aim of this study was to investigate the $ONOO^-$, NO, ${\cdot}{O_2}^-$ scavenging and NF-${\kappa}B$ related anti-inflammatory activities of Sotosaja-hwan in ob/ob mice. Methods: Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57/BL6J black mice) and control obese (ob/ob mice) groups have received standard chow. The experimental groups were fed with a diet of chow supplemented with 30 and 90 mg Sotosaja-hwan per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blotting was performed using anti-phospho-$I{\kappa}B$-${\alpha}$, anti-IKK-${\alpha}$, anti-NF-${\kappa}B$ (p50, p65), anti-COX-2, anti-iNOS, anti-YCAM-1 and anti-MMP-9 antibodies, respectively. Results: Sotosaja-hwan inhibited the generation of $ONOO^-$, NO and ${\cdot}{O_2}^-$ in the lipopolysaccharide (LPS)-treated mouse kidney postmitochondrial fraction in vitro. The generation of $ONOO^-$, NO, ${\cdot}{O_2}^-$ and PGE2 were inhibited in the Sotosaja-hwan-administered ob/ob mice groups. The GSH/GSSG ratio was decreased in the ob/ob mice, whereas the ratio was improved in the Sotosaja-hwan-administered groups. Sotosaja-hwan inhibited the protein expression levels of phospho-$I{\kappa}B$-${\alpha}$, IKK-${\alpha}$, NF-${\kappa}B$ (p50, p65), COX-2, iNOS, YCAM-1 and MMP-9 genes. Conclusions: These results suggest that Sotosaja-hwan is an effective $ONOO^-$, ${\cdot}{O_2}^-$ and NO scavenger and has NF-kB related anti-inflammatory activity in ob/ob mice. Therefore, Sotosaja-hwan might be a potential therapeutic drug against the inflammation process and inflammation-related diseases.

• Journal of the Korean Applied Science and Technology
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• v.31 no.1
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• pp.120-129
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• 2014

The structure and physiological function of human skin continuously weaken due to growing older. The reasons of aging from external conditions are long term exposure to sun, wind, heat, cigarette smoke, and etc. This also palmitoyl oligopeptide or ceramide oligopeptide are known asc ingredient stimulating collagens and have the effect of reproducing the upper level of skin. Acetyl hexapeptide is an ingredient that makes the skin and muscle suppler and reduces wrinkles. It is a major high function beauty ingredient that substitutes botox. After dividing 7%, 14%, and 20% Acetyl hexapeptide experimental groups as groups A, B, and C the control group and experiment groups' change of wrinkles, hair follicles, moisture content, and dead skin cells was analyzed. According to the results, Acetyl hexapeptide seems to affect wrinkles, hair follicles and moisture content contrasting to the control group. Experimental groups and control group showed similar change in dead skin cells. In contrast to the control group satisfaction of examines was affected in wrinkles, hair follicles and moisture but removing dead skin cells had similar result in experimental groups and control group.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.2
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• pp.82-87
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• 1983

The aging and growth of Spisula sachalinensis from Ingu over the period from December 1981 through November 1982 were studied. The rings on the shell were used as the character for age determination. The ring where the translucent zone shifts to the opaque one was regarded as an annulus. The time of its formation was estimated by monthly variations of marginal growth rate in the shell. It was formed once a year over the period from August through September. The shell length at the formation of the annulus was estimated by taking the mean shell length corresponding to each of the annual ring. From analysis of mean shell length at the formation of the annulus, von Bertalanffy's growth equation was estimated as follows; $l_t=126.38(1-e^{-0.262(t-0.656)})\;W_t=485.85(1-e^{-0.262(t-0.656)})^3$ Back-calculated shell lengths estimated from this equation was quite consistent with actual shell lengths.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.582-585
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• 2015

In the aging society, TKRA is steadily increased because of joint diseases. Artificial joint used in TKRA generates metal artifacts in the MRI. Metal artifact may affect diagnosis. In study, We are going to minimize the effect of metal artifact to improve the value of diagnosis by changing the sequence and the type of artificial joint(Co-Cr, Ni-Ti). 1.5T AVANTO, plastic containers and each of the artificial joint (Normal, Co-Cr, NiTi) were used. After the artificial joints fixed in a paper cup was inserted in a plastic container of cylindrical, Signal intensity was measured. To obtain strong and uniform signal intensity, the plastic container was filled with water. We changed Sequences(T1 TSE, T2 TSE, PD TSE) and obtained an Axial image. After excepting the maximum and minimum values, We calculated the average of SNR, CNR and PSNR. Consequently, The SNR, CNR value of PD TSE are measured higher than these of T1 TSE, T2 TSE and The PSNR of Co-Cr is higher than this of Ni-Ti. The SNR of Co-Cr is similar to the SNR of normal comparing this of Ni-Ti. As a result, Using sequence of PD Tse and Co-Cr alloy is considered to be useful.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.2C
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• pp.184-191
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• 2008

Oriental base diagnosis method is not going to hospital different from Oriental medicine and because of the possible disease diagnosis through the network and many communication equipment. Especially diagnosis development using ocular inspection method aspect of Oriental medicine among an aging society advance into cut the medical cost for contribution. Ocular inspection method the most important look at disease color that is Observing a Person's Shape and Color which is implementation the development of methodology and important the build of application ability system. So in this paper study observing a person's shape and color implementation of ocular inspection. Specially body's the five viscera presentate the five colors disease color in face that is important the color coordinate system thesis so that proceed the experiment for the color coordinate system analysis. Finally five color extract need the observing a person's shape and color through experiment select the digital color system and so real skin color analysis and comparison about the experiment which suggest the something to color coordinate system the best case of digital color system for observing a person's shape and color implementation.

• Biomedical Science Letters
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• v.18 no.1
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• pp.56-62
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• 2012

This study was to investigate the effects of high-fat diet feeding for a very long period of time on gene expression of inflammatory cytokines in mouse adipose tissue and to determine whether caloric restriction (CR) or insulin sensitizer treatment changes the cytokine gene expressions even in obese mice fed a high-fat diet for a very long term-period. Gene expression levels of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) were examined by real-time PCR in subcutaneous abdominal adipose tissue (SubQ) from obese and non-obese male C57BL/6 mice at 16, 26, 36, 47, and 77 weeks of age on either normal diet (ND) or high-fat diet (HFD) after starting at 6 weeks of age. In addition, gene expression levels of TNF-${\alpha}$, IL-6 and MCP-1 were determined in SubQ before and after rosiglitazone treatment or CR on 47-week-old obese mice. The results demonstrated that gene expression levels of TNF-${\alpha}$, IL-6 and MCP-1 were significantly increased with aging in SubQ of mice in both groups of diet. MCP-1 gene expression of SubQ in all ages tested was significantly or marginally increased in mice on HFD compared with ND. While TNF-${\alpha}$ expression was significantly reduced by rosiglitazone, IL-6 and MCP-1 were significantly decreased by CR. The basic data in this study will be useful for characterizing the C57BL/6 mouse as an animal model of obesity induced by high-fat diet feeding for a very long period of time, and a better understanding of inflammatory cytokine regulation in diet induced obesity which may facilitate the development of new therapeutic strategies to prevent the complications of obesity.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.3D
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• pp.413-420
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• 2011

An experiment was conducted to suggest the road pavement material combining wooden chip crushed from little useful roots and branches from logging sites or wood waste from construction sites with urethane resin. For the specimen, the mass ratio of urethane resin to construction wood waste chip/lumber waster chip was set to three different levels of 0.5, 0.75, and 1.0, which was measured, mixed with mixer, and molded; 7 days after, tensile strength test, elasticity test using golf balls and steel balls, permeability coefficient measurement, and flammability test were executed. As the result, the tensile strength of the specimen at the dry state in the air exhibited the range of 0.2-1.1MPa, and there was no change after 7 days of aging. When submerged in water, however, the strength was partially diminished; the diminishing rate was greater for less urethane resin usage, and therefore it appears desirable to set the mass ratio of resin to the wood waste chip over 0.75 to consider the moisture intrusion by precipitation and such. As the result of elasticity test, the GB and SB coefficients of the specimen using wood waste chips and urethane resin were measured to be low at below 20%, exhibiting excellent elasticity as road pavement material. Also, the permeability coefficient was over 0.5mm/sec for specimens of all combinations, exceeding the standard value required after construction for permeable pavement material, and the flammability of wood-type pavement material was evaluated to have no practical issues.

• Journal of Ginseng Research
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• v.39 no.4
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• pp.314-321
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• 2015

Background: Ginseng belongs to the genus Panax. Its main active ingredients are the ginsenosides. Interstitial cells of Cajal (ICCs) are the pacemaker cells of the gastrointestinal (GI) tract. To understand the effects of ginsenoside Re (GRe) on GI motility, the authors investigated its effects on the pacemaker activity of ICCs of the murine small intestine. Methods: Interstitial cells of Cajal were dissociated from mouse small intestines by enzymatic digestion. The whole-cell patch clamp configuration was used to record pacemaker potentials in cultured ICCs. Changes in cyclic guanosine monophosphate (cGMP) content induced by GRe were investigated. Results: Ginsenoside Re ($20-40{\mu}M$) decreased the amplitude and frequency of ICC pacemaker activity in a concentration-dependent manner. This action was blocked by guanosine 50-[${\beta}-thio$]diphosphate [a guanosine-5'-triphosphate (GTP)-binding protein inhibitor] and by glibenclamide [an adenosine triphosphate (ATP)-sensitive $K^{+}$ channel blocker]. To study the GRe-induced signaling pathway in ICCs, the effects of 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (a guanylate cyclase inhibitor) and RP-8-CPT-cGMPS (a protein kinase G inhibitor) were examined. Both inhibitors blocked the inhibitory effect of GRe on ICC pacemaker activity. L-NG-nitroarginine methyl ester ($100{\mu}M$), which is a nonselective nitric oxide synthase (NOS) inhibitor, blocked the effects of GRe on ICC pacemaker activity and GRe-stimulated cGMP production in ICCs. Conclusion: In cultured murine ICCs, GRe inhibits the pacemaker activity of ICCs via the ATP-sensitive potassium ($K^{+}$) channel and the cGMP/NO-dependent pathway. Ginsenoside Re may be a basis for developing novel spasmolytic agents to prevent or alleviate GI motility dysfunction.

• Phonetics and Speech Sciences
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• v.2 no.4
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• pp.245-252
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• 2010

Korea does not have a certain criteria on the respiratory ability and phonation of the normal aged, and also has no clear standard to examine the boundaries of geriatric diseases. This study analyzed the characteristics in respiration and phonation of the aged in normal healthy elderly from diverse angles with different variables. Thirty-three participants in total, seven males and eight females in the age group 55-64 participated in the study. Seven males and eleven females in the age group 65-74 were selected for the respiration and phonation experiments, and 10 different variables such as FVC, $FEV_1$, $FEV_1$/FVC, MPT, MFR, Psub, f0, jitter, shimmer and NHR were comparatively analyzed for each group of different age and gender. To see the difference in respiration and phonation by age and gender, the study conducted a two-way ANOVA. First, from the result of the analysis on respiratory ability, FVC of male appeared to be significantly greater than female. In both age groups of 55-64 and 65-74, male displayed greater FVC than female did. Second, as for $FEV_1$, $FEV_1$/FVC, the age group of 55-64 showed greater values than the values measured in the age group of 65-74. Third, MFR showed a significant difference by gender. In both age groups, male showed significantly higher MFR than female. Fourth, for different gender, a significant difference in MFR was observed. In both age groups of 55-64 and 65-74, male exhibited higher MFR than female. This study has a clinical implication in that it analyzed the criteria on respiration and phonation in normal healthy elderly according to gender and age. The normal aged showed a difference in their respiratory and phonatory functions by age and gender, it was closely related to the decline of pulmonary function due to the physical aging and the weak respiration coming from weakness of respiratory muscles. Also, the physical differences in height, weight, and the muscles in laryngeal and respiratory system between males and females had an influence on the performance. These results from this study might be a helpful guideline for the clinical criterion in the future.

• Nuclear Engineering and Technology
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• v.20 no.2
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• pp.88-104
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• 1988

The FUel Rod Analysis(FURA) code is developed using two-dimensional finite element methods for axisymmetric and plane stress analysis of fuel rod. It predicts the thermal and mechanical behavior of fuel rod during normal and load follow operations. To evaluate the exact temperature distribution and the inner gas pressure, the radial deformation of pellet and clad, the fission gas release are considered over the full-length of fuel rod. The thermal element equation is derived using Galerkin's techniques. The displacement element equation is derived using the principle of virtual works. The mechanical analysis can accommodate various components of strain: elastic, plastic, creep and thermal strain as well as strain due to swelling, relocation and densification. The 4-node quadratic isoparametric elements are adopted, and the geometric model is confined to a half-pellet-height region with the assumption that pellet-pellet interaction is symmetrical. The pellet cracking and crack healing, pellet-cladding interaction are modelled. The Newton-Raphson iteration with an implicit algorithm is applied to perform the analysis of non-linear material behavior accurately and stably. The pellet and cladding model has been compared with both analytical solutions and experimental results. The observed and predicted results are in good agreement. The general behavior of fuel rod is calculated by axisymmetric system and the cladding behavior against radial crack is used by plane stress system. The sensitivity of strain aging of PWR fuel cladding tube due to load following is evaluated in terms of linear power, load cycle frequency and amplitude.