• Design & Manufacturing
• /
• v.16 no.1
• /
• pp.43-49
• /
• 2022

As technologies in various industrial fields develop, high-quality parts are required. In the past, precision parts were produced by the contact machining method, but the contact machining method has clear limitations. In order to solve this problem, research on a non-contact processing method has been conducted, and laser processing and electric discharge processing are representative. However, the non-contact method has a problem in that productivity is insufficient, and there is a problem that it takes a lot of time to continuously process microholes. Researchers have developed an electron beam drilling equipment for continuous processing of fine holes, and a vaporization amplification sheet to increase the processing efficiency of the equipment. In this study, a cylindrical vaporization amplification sheet using room temperature curing type silicon was fabricated, and the metal distribution and thickness uniformity of the produced sheet were analyzed. In order to manufacture a cylindrical vaporization amplification sheet, an equipment capable of using centrifugal force was developed, and a sample in which metal powder was evenly distributed and a constant thickness was produced.

• Asia-Pacific Journal of Business
• /
• v.14 no.1
• /
• pp.113-129
• /
• 2023

Purpose - The purpose of this study is to examine whether trading volume amplifies the extent to which lottery-type stocks are overpriced, and whether economic sentiment index explains time-variation in the magnitude of the volume amplification effect. Design/methodology/approach - We examine monthly returns on 5x5 monthly bivariate portfolios formed by lottery characteristics (measured by maximum daily return) and trading volume. In addition, we perform time-series regression tests to examine how the volume amplification effect changes in high and low economic sentiment periods, after controlling for Fama-French three factors. Findings - Our bivariate portfolio analysis shows that the overpricing of lottery-type stocks are mostly pronounced among high trading volume stocks. In contrast, for low trading volume stocks, overpricing of lottery-type stocks appears to vanish. Furthermore, the amplification effect of trading volume on overpricing of lottery-type stock is concentrated in high economic sentiment periods. Research implications or Originality - This study is the first attempt to examine whether trading volume drives lottery-type stocks' overpricing in the Korean stock market. Furthermore, our analysis unveils the time-varying nature of volume amplification effect. The results suggest that trading volume might play a important hidden role in asset pricing, opening a new line of researches in the future.

• Korean Journal of Materials Research
• /
• v.34 no.2
• /
• pp.63-71
• /
• 2024

Slipchip offers advantages such as high-throughout, low cost, and simple operation, and therefore, it is one of the technologies with the greatest potential for high-throughput, single-cell, and single-molecule analyses. Slipchip devices have achieved remarkable advances over the past decades, with its simplified molecular diagnostics gaining particular attention, especially during the COVID-19 pandemic and in various infectious diseases scenarios. Medical testing based on nucleic acid amplification in the Slipchip has become a promising alternative simple and rapid diagnostic tool in field situations. Herein, we present a comprehensive review of Slipchip device advances in molecular diagnostics, highlighting its use in digital recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), and polymerase chain reaction (PCR). Slipchip technology allows users to conduct reliable droplet transfers with high-throughput potential for single-cell and molecule analyses. This review explores the device's versatility in miniaturized and rapid molecular diagnostics. A complete Slipchip device can be operated without special equipment or skilled handling, and provides high-throughput results in minimum settings. This review focuses on recent developments and Slipchip device challenges that need to be addressed for further advancements in microfluidics technology.

• Journal of Microbiology and Biotechnology
• /
• v.34 no.7
• /
• pp.1544-1549
• /
• 2024

This study presents a fluorescent mechanism for two-step amplification by combining two widely used techniques, exponential amplification reaction (EXPAR) and catalytic hairpin assembly (CHA). Pseudomonas aeruginosa (P. aeruginosa) engaged in competition with the complementary DNA in order to attach to the aptamer that had been fixed on the magnetic beads. The unbound complementary strand in the liquid above was utilized as a trigger sequence to initiate the protective-EXPAR (p-EXPAR) process, resulting in the generation of a substantial quantity of short single-stranded DNA (ssDNA). The amplified ssDNA can initiate the second CHA amplification process, resulting in the generation of many double-stranded DNA (dsDNA) products. The CHA reaction was initiated by the target/trigger DNA, resulting in the release of G-quadruplex sequences. These sequences have the ability to bond with the fluorescent amyloid dye thioflavin T (ThT), generating fluorescence signals. The method employed in this study demonstrated a detection limit of 16 CFU/ml and exhibited a strong linear correlation within the concentration range of 50 CFU/ml to 10⁵ CFU/ml. This method of signal amplification has been effectively utilized to create a fluorescent sensing platform without the need for labels, enabling the detection of P. aeruginosa with high sensitivity.

• Steel and Composite Structures
• /
• v.21 no.5
• /
• pp.1069-1084
• /
• 2016

This paper introduces a new and effective design amplification factor-based approach for reliable optimum design of trusses. This paper may be categorized as in the family of decoupled methods that aiming for a reliable optimum design based on a Design Amplification Factor (DAF). To reduce the computational expenses of reliability analysis, an improved version of Response Surface Method (RSM) was used. Having applied this approach to two planar and one spatial truss problems, it exhibited a satisfactory performance.

• 전기의세계
• /
• v.30 no.9
• /
• pp.582-588
• /
• 1981

The laser parameters such as a population inversion density, a stimulated emission cross section and loss factor in the glass laser amplifier medium were experimentally determined in order to analyse the properties of laser amplification. Using these parameters, the rate equations were approximately solved and the properties of laser amplification were analysed. An experiment of two-stage amplifier of Q-switching laser pulse was performed and the maximum output power was 1.1 GW, 33J. The several problems such as a laser solarization and a laser coupling in laser amplification were resolved.

• Journal of Photoscience
• /
• v.9 no.2
• /
• pp.338-340
• /
• 2002

In broom sorghum, Sorghum bicolor Moench, UV causes anthocyanin synthesis having action peaks in UVA and UVB regions. We previously reported that UV induces anthocyanin synthesis through UVB photoreceptor and phytochrome activated by UV. Furthermore, UVA and UVB amplify phytochrome-induced anthocyanin synthesis (PIAS). Our action- spectroscopic research indicated that a UV -receptor for amplification of PIAS is likely to be the same or same type of UVB photoreceptor for induction of anthocyanin synthesis. UVA-amplification of PIAS can be explained by the action of a cryptic red light signal (CRS), an amplification factor for PIAS produced by a distinct phytochrome-species activated by UVA. We suggest that UVA photoreceptors are not involved in anthocyanin synthesis in the broom sorghum.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.11
• /
• pp.1513-1518
• /
• 2005

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.

• Proceedings of the Earthquake Engineering Society of Korea Conference
• /
• 2002.03a
• /
• pp.51-58
• /
• 2002

The relative site amplification factors in southern Korea were determined from coda waves using coda normalization method. The seismograms of 15 events at 79 stations were used in this study. Seismogram envelopes were obtained by the Hilbert transform of bandpass-filtered velocity seismograms with frequency bands at 1-2Hz, 2-4Hz, 4-8Hz, 8-l6Hz and 16-32Hz. The envelopes were stabilized by application of moving-average scheme with time window of 1 second. The relative amplitudes of seismogram envelope were computed by dividing the amplitude of seismogram envelope at one site by the amplitude of seismogram envelope at reference site. The relative site amplification factors were obtained by taking averages of the relative amplitude. Values of relative site amplification factors in southern Korea are generally low in western area and high in eastern area.

• Journal of Microbiology and Biotechnology
• /
• v.28 no.5
• /
• pp.821-825
• /
• 2018

A copy number amplification system for yeast artificial chromosomes (YACs) was combined with simultaneous overexpression of genes integrated into a YAC. The chromosome VII (1,105 kb) was successfully split to 887 kb, 44 kb containing the element for copy number amplification, and a 184-kb split-YAC. The 44-kb split-mini YAC was amplified a maximum of 9-fold, and the activity of the reporter enzymes integrated into the split-mini YAC increased about 5-7-fold. These results demonstrate that the mini-YAC containing a targeted chromosome region can be readily amplified, and the specific genes in the mini-YAC could be overexpressed by increasing the copy number.