• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7997-8002
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• 2015

Gene amplification is an important mechanism in the development and progression of cancer. Currently, gene amplification status is generally determined by in situ hybridization (ISH). Multiplex ligation-dependent probe amplification (MLPA) is a PCR-based method that allows copy number detection of up to 50 nucleic acid sequences in one reaction. The aim of the present study was to compare results for HER2, CCND1, MYC and ESR1 gene amplification detected by MLPA with fluorescent in situ hybridization (FISH) and chromogenic in situ hybridization (CISH) as clinically approved methods. Tissue samples of 170 invasive breast cancers were collected. All were ER positive. Tissue samples had previously been tested for HER2 using immunohistochemistry. Amplification of the selected genes were assessed using MLPA, FISH and CISH and results were compared. HER2 MLPA and ISH results were also compared with HER2 immunohistochemistry (IHC) which detects protein overexpression. Amplification of HER2, CCND1, MYC and ESR1 by MLPA were found in 9%, 19%, 20% and 2% of samples, respectively. Amplification of HER2, CCND1, MYC and ESR1 by FISH was noted in 7%, 16%, 16% and 1% of samples, respectively. A high level of concordance was found between MLPA/FISH (HER2: 88%, CCND1: 88%, MYC: 86%, ESR1: 92%) and MLPA/CISH (HER2: 84%). Of all IHC 3+ cases, 91% were amplified by MLPA. In IHC 2+ group, 31% were MLPA amplified. In IHC 1+ group, 2% were MLPA amplified. None of the IHC 0 cases were amplified by MLPA. Our results indicate that there is a good correlation between MLPA, IHC and ISH results. Therefore, MLPA can serve as an alternative to ISH for detection of gene amplification.

• 생명과학회지
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• 제20권5호
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• pp.789-793
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• 2010

복잡한 진핵생물에서의 물리적 지도 작성이나 기능해석에 효모인공염색체(YAC)를 이용하기 위해서는 원하는 target region의 인공염색체화 및 single-copy인 YAC의 복제수를 늘이는 것이 요구된다. 본 연구에서는 YAC manipulation system에 복제수 증폭시스템(copy number amplification system)을 도입한 Simultaneous YAC Manipulation-Amplification (SYMA) system을 구축하였다. 식물염색체를 가진 YAC clone의 splitting과 증폭을 위해 conditional centromere와 thymidine kinase (TK) 유전자를 가진 pBGTK plasmid를 구축하였고, splitting fragment의 PCR을 위한 주형으로 사용하였다. 590 kb의 YAC clone은 splitting과 동시에 copy number amplification element를 가진 100 kb YAC와 490 kb YAC로 분리되었고, 100 kb YAC는 유도기질로 3 mg/ml sulfanilamide와 $50\;{\mu}g/ml$ methotrexate (S3/M50)의 첨가에 의해 14.4배로 그 복제수가 증가하였음을 확인할 수 있었다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2002년도 학술대회지
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• pp.831-834
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• 2002

An analytic approach is attempted to predict the amplification of turbulence in compressible flows experiencing one-dimensional and axisymmetric bulk dilatation. The variations of vortex radius and vorticity are calculated, and then the amplification of turbulence is obtained from them by tracking three representative vortices. For a one-dimensionally compressed flow, the present analysis slightly underestimates the amplification of velocity fluctuations and turbulent kinetic energy, relative to that of rapid distortion theory in the solenoidal limit. For an axisymmetrically distorted flow, the amplification of velocity fluctuations and turbulent kinetic energy depend not only on the density ratio but also on the ratio of streamwise mean velocities, which represents streamwise vortex contraction/stretching. In all flows considered, the amplification of turbulence is dictated by the mean density ratio. In the axisymmetric flow, streamwise vortex stretching/contraction, however, alters the amplification slightly.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2007년도 정기 학술대회 논문집
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• pp.777-782
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• 2007

Displacement amplification factor can be used to estimate inelastic displacement demands from elastic displacement demands, The simple formula for displacement amplification factor considering hysteretic behavior of structural system and earthquake characteristics is proposed. And the effects of several parameters such as displacement ductility, strain hardening ratio, period, characteristics of earthquakes and hysteretic models for the displacement amplification factor are evaluated. Accuracy of the proposed formula is evaluated by comparing the displacement amplification factors estimated by existing and proposed formula with those calculated from inelastic time history analysis. The displacement amplification factors by proposed formulas provide a good agreement with those calculated by inelastic time history analysis.

• Geomechanics and Engineering
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• 제3권3호
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• pp.189-206
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• 2011

Amplification based on empirical relations is widely used for seismic microzonation of urban centers. Amplifications are used to represent the site effects of a particular soil column. Many empirical correlations are available to estimate the amplification of seismic waves. These correlations are based on the ratio of shear wave velocity of foundation/rock to soil velocity or 30 m equivalent shear wave velocity ($Vs^{30}$) and are developed considering deep soil data. The aim of this work is to examine the applicability of available amplification relations in the literature for shallow engineering bedrock sites by carrying out site response studies. Shear wave velocity of thirteen sites having shallow engineering bedrock have been selected for the study. In these locations, the depth of engineering bedrock (> 760 ${\pm}$ 60 m/s) is matched with the drilled bore hole. Shear wave velocity (SWV) has been measured using Multichannel Analysis of Surface Wave survey. These sites are classified according to the National Earthquake Hazards Reduction Program (NEHRP) classification system. Amplifications for an earthquake are arrived for these sites using empirical relations and measured SWV data. Site response analysis has been carried out in SHAKE using SWV and using synthetic and real earthquake data. Amplification from site response analysis and empirical relations are compared. Study shows that the amplification arrived using empirical relations does not match with the site response amplification. Site response amplification is much more than empirical values for same shear wave velocity.

• Journal of Preventive Medicine and Public Health
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• 제45권4호
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• pp.235-243
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• 2012

Objectives: Nail has been a substitute DNA source for genotyping. To investigate the integrity and consistency of nail DNA amplification for biomarker study, nail clippings from 12 subjects were collected at monthly intervals. The possibility of longer amplification and existence of GAPDH RNA/protein, were also investigated with three nail samples. Methods: Three primer sets were designed for quantitative amplification of nuclear and mitochondrial genes and analysis of their consistency. The mean threshold cycles in amplification of the target genes were compared to test the consistency of polymerase chain reaction (PCR) performance among individual factors including age groups, sex, family, the nail source, and by the size of the amplification segments. Results: The amplification of the target genes from nail DNA showed similar integrity and consistency between the nail sources, and among the serial collections. However, nail DNA from those in their forties showed earlier threshold cycles in amplification than those in their teens or seventies. Mitochondrial DNA (mtDNA) showed better DNA integrity and consistency in amplification of all three targets than did nuclear DNA (nucDNA). Over 9 kb of mtDNA was successfully amplified, and nested quantitative PCR showed reliable copy numbers (%) between the two loci. Reverse transcription PCR for mRNA and immunoblotting for GAPDH protein successfully reflected their corresponding amounts. Regarding the existence of RNA and protein in nails, more effective extraction and detection methods need to be set up to validate the feasibility in biomarker study. Conclusions: Nail DNA might be a feasible intra-individual monitoring biomarker. Considering integrity and consistency in target amplification, mtDNA would be a better target for biomarker research than nucDNA.

• 한국위성정보통신학회논문지
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• 제12권4호
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• pp.152-156
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• 2017

본 논문은 양자 키 분배 시스템에서의 보안성을 증폭시키기 위한 랜덤 보안성 증폭의 개념을 소개한다. 양자 오류 정정과 보안 사이의 관계를 이용하여 보안성 증폭을 제공하는 것을 보인다. 또한 보안성 증폭 측면에서 랜덤 보안성 증폭이 기존 유니버셜 해쉬 함수만을 이용한 것보다 나은 보안성을 제시한다는 것을 보인다. QKD의 대표적인 예인 BB84 프로토콜을 이용하여 유니버셜 해시 함수가 보안성을 강화하는 과정을 설명한다. 마지막으로 제안한 랜덤 보안성 증폭과 기존 방식이 양자 QKD 채널에서 키 생성률에 따른 보안성을 비교한다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2005년도 춘계 학술발표회 논문집
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• pp.1134-1139
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• 2005

Seismic motion amplification owing to the input motion level at bedrock is one of the important topics to understand various geomaterials behavior. The extremely valuable borehole records at Port Island were obtained during the 1995 Hyogoken Nanbu Earthquake and also before and after the main event. In this study, the seismic motion amplification at the soft reclaimed ground was discussed. Comparison of measured records with numerical simulation results were made with focus on seismic motion amplification characteristics at the soft reclaimed ground.

• Current Optics and Photonics
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• 제6권6호
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• pp.576-582
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• 2022

Plasmonic high-order harmonic generation (HHG) is used in nanoscale optical applications because it can help in realizing a compact coherent ultrashort pulse generator on the nanoscale, using plasmonic field enhancement. The plasmonic amplification of nanostructures induces nonlinear optical phenomena such as second-order harmonic generation, third-order harmonic generation, frequency mixing, and HHG. This amplification also causes damage to the structure itself. In this study, the plasmonic amplification according to the design of a metal-coated sapphire conical structure is theoretically calculated, and we analyze the effects of this optical amplification on HHG and damage to the sample.

• 한국식품과학회지
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• 제48권2호
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• pp.128-132
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• 2016

PEG를 이용하여 WGA 수행 시 DNA 증폭 효율을 높이고 이를 식중독 세균의 DNA 증폭 및 검출에 적용하고자 하였다. 등온 증폭 반응인 WGA에 여러 종류의 PEG를 첨가하여 증폭한 결과, 1.5% 농도의 PEG 4,000을 첨가하는 것이 가장 효율이 높음을 알 수 있었다. 증폭 정도를 정량적으로 파악하기 위하여 3종의 식중독 세균 DNA를 이용하여 WGA를 수행하였으며 real-time PCR로 정량분석하였다. S. Typhimurium, L. monocytogenes, V. parahaemolyticus의 경우에 WGA를 하지 않은 DNA에 비하여 각각 7,777.01배, 9,981.22배, 1,239.03배 정도로 DNA의 양이 증폭되는 것을 확인하였다. 또한 PEG를 첨가함으로써 18배에서 40배의 핵산 증폭 효과가 더 있음을 알 수 있었다. 따라서 식품에 미량의 농도로 존재하는 식중독 세균은 PEG가 첨가된 WGA 반응을 통하여 검출 가능성을 높일 수 있음을 알 수 있었다.