• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.957-965
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• 2005

The effect of ammoniation with urea and with soybean meal (SBM) as a source of urease on the nutritive value of wheat straw was evaluated in sheep. Twenty-four male Najdi lambs were used in a 3${\times}$2 factorial design, in which the animals were allocated to three straw treatments: 0% urea-treated (NT), 6% urea-treated (UT) and 2.2% urea-supplemented (US) straws. Each straw treatment was either supplemented or non-supplemented with 70 g SBM $kg^{-1}$ straw during the treatment time with urea, giving a total of six straw treatments. Each of these treatments was individually fed ad libitum to 4 lambs, together with 300 g of barley grain/head/day. Total N content of UT and US straws increased significantly (p<0.001) as compared to NT straw. The degree of urea hydrolysis, either with or without SBM addition, was nearly similar. Lambs fed either UT or US straw based diets had significantly (p<0.01) and numerically (p>0.05) higher straw DM intake (g $d^{-1}$ $kg^{-1}$ $BW^{0.75}$), compared to those fed NT straw based diet. Apparent DM or OM digestibilities increased significantly (p = 0.014) in lambs fed UT diet, and numerically (p>0.05) in lambs fed US diet as compared to those fed NT diet. Fiber (CF, NDF, ADF, cellulose and hemicellulose) digestibility increased to a similar magnitude, averaging 20.2 (p<0.001) and 7.8% (p<0.07); this corresponds to 35 (p<0.001) and 51% (p<0.001) in N digestibility and approximately 78 (p<0.017) and 105% (p<0.002) in N retention, for UT and US diets, respectively, as compared to NT diet. However, the UT diet had higher (p<0.01) fiber digestibility over the US diet. Addition of SBM tended to improve (p = 0.09) straw DM and digestible OM intakes, while significantly increasing (p<0.001) total and digestible CP intakes across all diets. Lambs fed on US diet had higher ruminal ammonia N than those fed on UT (p<0.05) or NT (p<0.001) diets. However, ruminal pH and molar proportion of the volatile fatty acids did not differ (p>0.05) among the treatment diets. This study suggests that US and UT treatments, particularly the latter, improved straw intake, digestibility and N utilization by lambs compared to NT treatment. On the other hand, addition of SBM as a source of urease had a negligible effect on urea hydrolysis.

• Asian-Australasian Journal of Animal Sciences
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• 제14권12호
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• pp.1690-1695
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• 2001

Wheat straw was treated on laboratory scale with 4% urea at a moisture level of 50% along with different amounts of acetic acid (AA) to fix various levels of ammonia nitrogen (15, 30, 45 and 60%) evolved from urea and stored for 4 weeks. Chemical composition of the treated samples revealed a significant (p<0.01) increase in N content of the samples where AA was added. The N content of the ammoniated straw was only 1.21% which increased to 2.58 with the addition of AA to trap 30% $NH_3-N$. The concentration of free $NH_3-N$ in the straw was significantly (p<0.01) less when more than 15% $NH_3-N$ was trapped with AA. There was significant increase (p<0.01) in N disappearance and depression in NDF and hemicellulose disappearance, when AA was used to trap 30% $NH_3-N$. Large scale treatment of wheat straw with 4% urea at a moisture level of 50% along with AA (to trap 30% $NH_3-N$) increased the N content, but not as much as in laboratory scale treatment. In vivo experiment conducted on nine adult male buffaloes divided into three groups revealed no difference in the intake of DM, OM, NDF, ADF, cellulose and hemicellulose among group I (ammoniated straw), group II (AA treated ammoniated straw) and group III (AA treated ammoniated straw +1 kg barley grain), but the intake was significantly (p<0.05) more in groups where AA treated straw was fed as compared to only ammoniated straw fed group. However EE digestibility was depressed in group II. The digestibility of cellulose and hemicellulose both depressed significantly (p<0.05) in group II and III as compared to group I. Animals in all the 3 groups showed positive nitrogen balance and it was significantly more in group II and III as compared to group I. DCP intake was significantly (p<0.05) more in group II and III as compared to group I, but there was no significant difference among the three groups in TDN intake. It can be concluded that AA (to trap 30% $NH_3-N$) is effective in capturing the excess ammonia released during urea ammoniation of straw and improving its nutritive value, as well as animal performance.

• Asian-Australasian Journal of Animal Sciences
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• 제19권12호
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• pp.1776-1783
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• 2006

Despite being a rich source of protein (28-34%), karanj (Pongamia glabra) cake is found to be bitter in taste and toxic in nature owing to the presence of flavonoid (karanjin), tannin and trypsin inhibitor, thereby restricting its safe inclusion in poultry rations. Feeding of karanj cake at higher levels (>10%) adversely affected the growth performance of poultry due to the presence of these toxic factors. Therefore, efforts were made to detoxify karanj cake by various physico-chemical methods such as dry heat, water washing, pressure cooking, alkali and acid treatments and microbiological treatment with Sacchraromyces cerevisiae (strain S-49). The level of residual karanjin in raw and variously processed cake was quantified by high performance liquid chromatography and tannin and trypsin inhibitor was quantified by titrametric and colorimetric methods, respectively. The karanjin, tannin and trypsin inhibitor levels in such solvent and expeller pressed karanj cake were 0.132, 3.766 and 6.550 and 0.324, 3.172 and 8.513%, respectively. Pressure-cooking of solvent extracted karanj cake (SKC) substantially reduced the karanjin content at a cake:water ratio of 1:0.5 with 30-minute cooking. Among chemical methods, 1.5% (w/w) NaOH was very effective in reducing the karanjin content. $Ca(OH)_2$ treatment was also equally effective in karanjin reduction, but at a higher concentration of 3.0% (w/w). A similar trend was noticed with respect to treatment of expeller pressed karanj cake (EKC). Pressure cooking of EKC was effective in reducing the karanjin level of the cake. Among chemical methods alkali treatment [2% (w/w) NaOH] substantially reduced the karanjin levels of the cake. Other methods such as water washing, dry heat, HCl, glacial acetic acid, urea-ammoniation, combined acid and alkali, and microbiological treatments marginally reduced the karanjin concentration of SKC and EKC. Treatment of both SKC and EKC with 1.5% and 2.0% NaOH (w/w) was the most effective method in reducing the tannin content. Among the various methods of detoxification, dry heat, pressure cooking and microbiological treatment with Saccharomyces cerevisiae were substantially effective in reducing the trypsin inhibitor activity in both SKC and EKC. Based on reduction in karanjin, in addition to tannin and trypsin inhibitor activity, detoxification of SKC with either 1.5% NaOH or 3% $Ca(OH)_2$, w/w) and with 2% NaOH were more effective. Despite the effectiveness of pressure cooking in reducing the karanjin content, it could not be recommended for detoxification because of the practical difficulties in adopting the technology as well as for economic considerations.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.325-329
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• 2004

Ten male buffalo calves (aged 6-8 months, average body wt. $88.5{\pm}0.5kg$) were divided into two groups of five animals in each. All the animals were fed on urea-ammoniated wheat straw (4% urea, 50% moisture) along with concentrate mixture (50:50 on DM basis). In addition animals in group II were given sodium sulphate to see the effect of sulphur on the utilization of nitrogen added through urea-ammoniation. This feeding practice continued for a period of 120 d, during which fortnightly body weights were taken to assess their growth rate. A metabolism trial was conducted after 90 days of feeding to know the digestibility of nutrients and their balance. Results revealed no significant difference in the intake of DM and other nutrients in two groups. The digestibility of DM, OM, EE, NDF, ADF and cellulose was alike in animals fed ammoniated straw and ammoniated straw+sodium sulphate supplemented group, whereas the digestibilities of CP and hemicellulose was significantly (p<0.01) more in group I and II respectively. There was no significant difference in intake of nitrogen, calcium and phosphorus in 2 groups. Similarly, the balance of these 3 nutrients was positive and statistically alike in two groups. Intake and excretion of sulphur through faeces and urine was significantly (p<0.01) more in group II than in group I. Inspite of higher excretion of sulphur through faeces and urine in group II, the sulphur balance was significantly (p<0.05) more in group II than in group I, probably due to significantly (p<0.01) higher intake of sulphur in this group. There was no significant difference in total body weight gain or average daily gain between two groups, indicating that addition of sodium sulphate did not have any positive effect on these parameters. Similarly the intake of DM, DCP and TDN were also alike in two groups. The DCP and TDN values of the two diets were 8.0, 60.4 and 6.8, 56.6% respectively. Feeding cost/unit gain was alike in both the groups.

• 한국가금학회지
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• 제13권2호
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• pp.221-226
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• 1986

분쇄옥수수에 수분을 조정(20%)하여 3$0^{\circ}C$에서 20일간 배양했을 때 발생한 곰팡이 수의 변화와 aflatoxin의 발생여부를 조사하였으며 곰팡이가 발생한 옥수수(moldy corn)를 7% 함유한 사료에 각종약품을 첨가시 broiler 병아리의 성장과 각종 장기에 미치는 영향을 측정하기 위하여 broiler 36수를 공시하여 대조구로 Fresh corn구, Moldy corn 구와 Ammonia 처리(1.5%) moldy corn구, Moldy corn＋BHT(butylated hydroxy toluene, 0.1%) 구, Moldy corn＋CTC(chlortetracycline, 100ppm)구, Moldy corn＋Probiotics(시판-P제판, 0.2%)구로 나누어 처리당 6수를 2수씩 3반복으로 하여 battery에 완전 임의배치하였다. 옥수수의 곰팡이 수는 수분을 첨가하지 않은 옥수수에서 11$\times$$10^4$/g, Moldy corn에서는 42$\times$$10^{7}$ g이 검출되었으며 TLC에 의한 검사에서 aflatoxin(B$_1$, B$_2$, G$_1$, G$_2$)은 검출되지 않았다. 4주간의 사양실험에서 얻어진 결과에 의하면 증체량, 사료섭취량 및 사료효율에 있어 각 처리간에 통계적 유의차는 없었으나 증체량에 있어 Ammonia처리구와 Probiotics 및 CTC 첨가구가 Moldy corn 구에 비하여 4-7%의 개선효과가 있었으며 사료효율에 있어서는 Probiotics 및 CTC 첨가구가 Moldy corn구보다 좋은 경향을 나타내었다. 간, 신장, 취장, 비장의 무게는 처리간에 유의한 차이가 없었다. 간의 건물함량은 Ammoniated moldy corn구와 Moldy corn＋BHT구가 Moldy corn 이외의 타처리구에 비하여 유의하게 (P〈0.05) 낮았으며 간의 단백질함량은 Moldy corn구와 Moldy corn＋CTC구가 Mold corn＋BHT구 이외의 타 처리구보다 유의하게 낮았다.(P〈0.05). 간의 조지방함량은 각 처리구간에 통계적 유의차는 없었으나 Moldy corn구가 타 처리구보다 높은 경향이 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권2호
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• pp.185-191
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• 2005

An experiment was conducted to study the effect of feeding ammoniated wheat straw treated with HCl on blood biochemical profiles in growing male buffalo (Bubalus bubalis) calves. Twenty-four growing male buffalo calves (one year of age, 88.54${\pm}$3.81 kg average body weight) were divided into three groups in a completely randomized design on the basis of their body weight. Animals in all the three groups were fed on concentrate mixture. In addition, they were offered wheat straw, ammoniated wheat straw (4% urea at 50% moisture level) and HCI treated ammoniated wheat straw (4% urea at 50% moisture level and HCl added to trap 30% of the NH3 evolved) in groups I, II and III, respectively for a period of 180 days, as per Kearl (1982) for body weight gain of 500 g/d. In all diets, concentrate:roughage ratio was fixed at 50:50 and were made isonitrogenous by adjusting CP levels of conc. mixtures. Blood was collected from jugular vein of each buffalo calf at the beginning and subsequently at two months interval of experimental feeding. Due to urea-ammoniation, the CP content of wheat straw increased from 2.90 to 6.96% and addition of HCl along with urea further increased the CP content to 10.09%. In all the three groups, the mean values of plasma glucose (mg %) and serum globulin (g %), showed a decreasing trend, while the mean value of serum TP (g %), serum A:G ratio, serum urea (mg %), serum creatinine (mg %), serum ALP (KA units), SGOT (units/ml.), SGPT (units/ml), serum T$_3$ and T$_4$ (ng/ml) showed an increasing trend with the advancement of feeding period. The cumulative period mean values of serum TP (6.15 to 6.20 g %), serum albumin (3.07 to 3.18, g %), serum globulin (2.98 to 3.09, g %), serum A:G ratio (1.03 to 1.10), serum ALP (23.15 to 23.63, KA units), serum T$_3$ (1.20 to 1.23 ng/ml) and serum T$_4$ (21.33 to 21.88 ng/ml) were comparable among the groups. The cumulative period mean plasma glucose (mg %) in group III (57.28) was similar to groups I (55.31) and II (59.41), however, the cumulative period mean plasma glucose in group II was significantly (p<0.01) higher than group I. The cumulative period mean serum urea (mg %) in group III (47.34) was significantly (p<0.001) higher than group I (38.38) and II (42.24), which were statistically alike. However, the cumulative period mean serum creatinine values (mg %) in groups II (1.43) and III (1.52) were similar and were significantly (p<0.01) higher than group I (1.24). The cumulative period mean SGOT (units/ml) in groups I, II and III was 91.71, 96.04 and 96.64, respectively. Similarly the cumulative period mean SGPT (units/ml) was 19.00, 19.93 and 20.01 in groups I, II and III, respectively. The cumulative period mean values of SGOT (p<0.05) and SGPT (p<0.001) in groups II and III were similar and were significantly higher than group I. The cumulative period mean serum T$_3$ and T$_4$ values in groups I (1.21 and 21.81), II (1.23 and 21.42) and III (1.20 and 21.33) were comparable. From the present study it may be concluded that feeding of AWS treated with and without HCI to growing male buffalo calves for 180 days had no significant adverse effect on blood biochemical profile.