• Title/Summary/Keyword: Ammonia exposure

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Thick-film ammonia gas sensor with high sensitivity and excellent selectivity

  • Lee, Kyuchung;Ryu, Kwang-Ryul;Hur, Chang-Wu
    • Journal of information and communication convergence engineering
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    • v.2 no.1
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    • pp.22-25
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    • 2004
  • A highly sensitive ammonia gas sensor using thick-film technology has been fabricated and examined. The sensing material of the gas sensor is FeOx-$WO_{3}-SnO_{2}$ oxide semiconductor. The sensor exhibits resistance increase upon exposure to low concentration of ammonia gas. The resistance of the sensor is decreased, on the other hand, for exposure to reducing gases such as ethyl alcohol, methane, propane and carbon monoxide. A novel method for detecting ammonia gas quite selectively utilizing a sensor array consisting of an ammonia gas sensor and a compensation element has been proposed and developed. The compensation element is a Pt-doped $WO_{3}-SnO_{2}$gas sensor which shows opposite direction of resistance change in comparison with the ammonia gas sensor upon exposure to ammonia gas. Excellent selectivity has been achieved using the sensor array having two sensing elements.

Toxic effects of ammonia exposure on growth performance, hematological parameters, and plasma components in rockfish, Sebastes schlegelii, during thermal stress

  • Shin, Ki Won;Kim, Shin-Hu;Kim, Jun-Hwan;Hwang, Seong Don;Kang, Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.19 no.10
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    • pp.44.1-44.8
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    • 2016
  • Rockfish, Sebastes schlegelii (mean length $14.53{\pm}1.14cm$ and mean weight $38.36{\pm}3.45g$), were exposed for 4 weeks with the different levels of ammonia in the concentrations of 0, 0.1, 0.5, and 1.0 mg/L at 19 and $24^{\circ}C$. The indicators of growth performance such as daily length gain, daily weight gain, condition factor, and hematosomatic index were significantly reduced by the ammonia exposure and high temperature. The ammonia exposure induced a significant decrease in hematological parameters, such as red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin (Hb), and hematocrit (Ht), whose trend was more remarkable at $24^{\circ}C$. Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were also notably decreased by the ammonia exposure. Blood ammonia concentration was considerably increased by the ammonia concentration exposure. In the serum components, the glucose, glutamic oxalate transaminase (GOT), and glutamic pyruvate transaminase (GPT) were substantially increased by the ammonia exposure, whereas total protein was significantly decreased. But, the calcium and magnesium were not considerably changed.

Effects of ammonia gas on soybean plant (대두(大豆)에 대한 Ammonia 가스의 영향)

  • Kim, B.Y.;Han, K.H.;Kim, S.K.
    • Korean Journal of Soil Science and Fertilizer
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    • v.12 no.2
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    • pp.109-116
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    • 1979
  • This experiment was conducted to find out the effects on soybean plant exposed with various concentration and different fumigation periods of ammonia gas ($0.2mg/{\ell}$). The yield of soybean, nitrogen and, chlorophyll contents in leaves, and percentage of destroyed leaf area were investigated. The results were summarized as: 1. The soybean yield losses and percentage of destroyed leaf area were positively correlated with concentration of inflicting ammonia gas respectively. The yield losses was higher at noon exposure time than at night exposure time. 2. The soybean yield was negatively correlated with the percentage of destroyed leaf area. 3. The highest percentage of destroyed leaf area had at afternoon exposure time (14:00-15:00 o'clock) by $0.2mg/{\ell}$ ammonia gas fumigation, and the lowest one had at midnight exposure time (22:00-23:00 o'clock). 4. According to the increasing concentration of ammonia gas, the total and water soluble nitrogen contents in soybean leaves were increased, but the contents of chlorphyll b on decreasing rate. 5. The nitrogen contents in plant were higher in the afternoon exposure than in the morning, but the contents of chlorophyll were higher at night time exposure than at day time. 6. The highest decreasing of absorption spectra of chlorohpyll in chloroplast had at 10:00-11:00 o'clock on fumigation time and the lowest one had at 22:00-23:00 o'clock.

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Alterations in Hematological Parameters and Antioxidant Responses in the Biofloc-reared Flatfish Paralichthys olivaceus Following Ammonia Exposure (암모니아 급성노출에 의한 바이오플락 사육 넙치(Paralichthys olivaceus)의 혈액학적 성상 및 항산화반응의 변화)

  • Kim, Jun-Hwan;Kim, Ki Wook;Bae, Sun-Hye;Kim, Su-Kyoung;Kim, Su Kyoung;Kim, Jong-Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.750-755
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    • 2017
  • Flatfish Paralichthys olivaceus raised in biofloc system (mean length $27.6{\pm}3.1cm$, mean weight $280.4{\pm}26.5g$) were exposed for to different concentrations of ammonia (0, 8, 16, 32, and $64mg\;{NH_4}^+/L$) for 7 days. Following ammonia exposure, hematological parameters such as hemoglobin and hematocrit were significantly reduced. Plasma components such as magnesium, glucose, aspartate aminotransferase (AST), and alanine aminotransaminase (ALT) were significantly altered by ammonia exposure, whereas there were no significant changes in calcium, cholesterol, or total protein. Antioxidant responses, such as superoxide dismutase (SOD) and catalase (CAT) levels were significantly elevated following ammonia exposure. The results of this study indicate that ammonia exposure induces significant changes in hematological parameters and antioxidant responses in biofloc-reared Paralichthys olivaceus as a toxic response.

Changes of hematological parameters and plasma components in the hybrid grouper(Epinephelus fuscoguttatus ♀×E. lanceolatus ♂) by acute ammonia exposure (암모니아 급성노출에 따른 대왕범바리(Epinephelus fuscoguttatus ♀×E. lanceolatus ♂)의 혈액성상 및 혈장성분의 변화)

  • Kim, Kwang-Min;Lee, Hee-Jin;Yun, Han Bin;Cho, Jae-Hwang;Kim, Seok-Ryel;Lee, Kyung Mi;Kim, Jun-Hwan
    • Korean Journal of Environmental Biology
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    • v.38 no.1
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    • pp.40-46
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    • 2020
  • Hybrid grouper(Epinephelus fuscoguttatus ♀×E. lanceolatus ♂) (mean weight 25.7±3.5g, mean length 11.2±0.9cm) were exposed to different ammonia concentrations of 0, 5, 10, 20, and 40mg L-1 for 96 hours. The hematological hematocrit and hemoglobin parameters of the hybrid grouper were significantly decreased by 20 mg L-1 ammonia exposure. In the organic plasma components, calcium was significantly decreased, whereas there was no change in magnesium. In the organic plasma components, the glucose and cholesterol values of the hybrid grouper were significantly increased by ammonia exposure. In the enzymatic plasma components, the ALP(Alkaline phosphatase) value of the hybrid grouper was also significantly increased by ammonia exposure. The results of this study demonstrate that acute ammonia exposure to hybrid grouper induced changes in hematological parameters and plasma components. Therefore, acute ammonia exposure over 20 mg L-1 appears to be toxic to hybrid grouper and the results can be used as a major indicator in breeding hybrid grouper.

Alterations of growth performance, hematological parameters, and plasma constituents in the sablefish, Anoplopoma fimbria depending on ammonia concentrations

  • Kim, un-Hwan;Park, Hee-Ju;Hwang, In-Ki;Han, Jae-Min;Kim, Do-Hyung;Oh, Chul Woong;Lee, Jung Sick;Kang, Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.20 no.3
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    • pp.4.1-4.6
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    • 2017
  • Juvenile Anoplopoma fimbria (mean length $16.8{\pm}2.2cm$, and mean weight $72.8{\pm}5.4g$) were exposed for 2 months with different levels of ammonia (0, 0.25, 0.50, 0.75, 1.00, and 1.25 mg/L). Growth performances such as daily length gain, daily weight gain, condition factor, and hepatosomatic index were significantly decreased by ammonia exposure. Hematological parameters such as red blood cell (RBC) count, hematocrit, and hemoglobin were also significantly decreased. In plasma inorganic components, calcium and magnesium were significantly decreased by ammonia exposure. In plasma organic components, there was no alteration in cholesterol and total protein. In enzyme plasma components, glutamic oxalate transaminase (GOT) and glutamic pyruvate transaminase (GPT) were significantly increased. The results of this study indicated that ammonia exposure can induce significant growth reduction and blood biochemistry alterations of A. fimbria.

Physiological Responses in Korean Rockfish (Sebastes schlegeli) Exposed to Ammonia (암모니아 노출에 따른 조피볼락(Sebastes schlegeli)의 생리학적 반응)

  • Min, Byung Hwa;Park, Mi Seon;Shin, Yun Kyung;Do, Yong Hyun;Myeong, Jeong-In
    • Korean Journal of Environmental Biology
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    • v.32 no.4
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    • pp.344-352
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    • 2014
  • The aim of the present study was to assess the effects of ammonia on physiological responses in Korean rockfish (Sebastes schlegeli). Interestingly, no mortality were observed when the specimens ($301.1{\pm}8.0g$) were exposed to five levels of un-ionized ammonia ($NH_3$) (control, 1, 2, 4, $8mg\;L^{-1}$) for 3 hours. Furthermore, a significantly higher increase in gill $Na^+/K^+$-ATPase (NKA) pump activity with was detected due to the ammonia exposure. The activity of the fishes were found to be 4 and $8mg\;L^{-1}$ $NH_3$, which was significantly high compared to normal $1mg\;L^{-1}$ $NH_3$. Although ammonia exposure had no effect on plasma $Cl^-$, exposure to both 4 and $8mg\;L^{-1}$ $NH_3$, however it led to increase in the plasma $Na^+$, $K^+$ and osmolality levels. Also, prolong ammonia exposure cause increase of plasma cortisol and glucose levels. The increase in glucose was accompanied by an increase in cortisol. The fish exposed to 4 and $8mg\;L^{-1}$ $NH_3$ showed significantly higher hematocrit than control group than those exposed to 1 and $2mg\;L^{-1}$ $NH_3$. The intensity of cell damage increased with the increase concentration and exposure to ammonia. Furthermore, hyperplasia, separation and epithelial necrosis were also observed in gill tissues. Taken together, the results showed that direction of changes to the investigated parameters can be used to determine the physiological responses of Korean rockfish to ammonia.

Effects on lethal concentration 50%, hematological parameters and plasma components of mirror carp, Cyprinus carpio nudus exposed to waterborne ammonia (수인성 암모니아 급성노출에 의한 향어, Cyprinus carpio nudus의 반수치사농도, 혈액학적 성상 및 혈장성분의 영향)

  • Jeong, Ji-Ho;Eun, Ji-Su;Joo, Chang-Hoon;Jo, A-Hyun;Hong, Su-Min;Kim, Jun-Hwan
    • Journal of fish pathology
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    • v.35 no.1
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    • pp.65-75
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    • 2022
  • Mirror carp, Cyprinus carpio nudus (weight 26.2±3.1 g, length 11.8±0.5 cm) were exposed to waterborne total ammonia nitrogen (TAN) at 0, 2, 4, 8, 16, 32, 64 and 128 mg TAN/L. The lethal concentration 50 (LC50) of mirror carp, C. carpio nudus exposed to waterborne ammonia was 60.38 mg TAN/L. The red blood cell (RBC) count was significantly reduced by ammonia exposure, whereas there was no significant changes in the hemoglobin concentration and hematocrit value. In the plasma components, glucose, total protein, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased by ammonia exposure. The results of this study suggest that the ammonia exposure to C. carpio nudus affects the survival rates, hematological parameters and plasma components as toxicity.

Fabrication and Characterization of Thick Film Ammonia Gas Sensor (후막형 암모니아 가스 센서의 제조 및 가스 감응 특성)

  • Yun, Dong-Hyun;Kwon, Chul-Han;Hong, Hyung-Ki;Kim, Seung-Ryeol;Lee, Kyu-Chung
    • Journal of Sensor Science and Technology
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    • v.6 no.6
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    • pp.445-450
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    • 1997
  • An ammonia gas sensor with high sensitivity using thick-film technology were fabricated and examined. The material for sensing the ammonia gas was the mixture of oxide semiconductor, $FeO_{x}-WO_{3}-SnO_{2}$. The sensor exhibits resistance increase upon exposure to low concentration of ammonia gas. The resistance of the sensor is decreased, on the other hand, for exposure to reducing gases such as ethyl alcohol, methane, propane and carbon monoxide. A novel method for detecting ammonia gas quite selectively utilizing a sensor array consisting of an ammonia gas sensor and a compensation element were proposed and developed. The compensation element is a Pt-doped $WO_{3}-SnO_{2}$ gas sensor which shows opposite direction of resistance change in comparison with that of the ammonia gas sensor upon exposure to ammonia gas. Excellent selectivity has been achieved using the sensor array having two sensing elements.

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Effects of Ammonia Exposure on Survival Rate and Hematological Characteristics Changes in Juveniles of Sevenband Grouper, Epinephelus septemfasciatus (능성어 (Epinephelus septemfasciatus) 치어의 생존율과 혈액학적 특성 변화에 미치는 암모니아 노출의 영향)

  • Kim, Jung Hyun;Park, Jong Youn;Lee, Jeong Yong;Lee, Jin Hwan;Hwang, Hyung Kyu;Cho, Jae Kwon
    • Korean Journal of Ichthyology
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    • v.29 no.1
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    • pp.13-21
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    • 2017
  • We investigated that changes of survival rate and hematological factors in sevenband grouper (Epinephelus septemfasciatus), exposed to ammonia. First, the fish was exposed and was observed survival rate. We observed the survival rate of the fish when exposed at each ammonia concentrations (0, 0.35, 0.4, 0.5, 0.8 mg/L, $NH_3$) for 96 hours. The 96-h $LC_{50}$ (lethal ambient concentration for 50% of the population) of $NH_4^+$ and un-ionized ammonia ($NH_3$) for sevenband grouper were 26.7 and 0.6 mg/L, respectively. Secondly, we exposed the fish to 96-h $LC_{50}$ ammonia concentration (0.6 mg/L) for 96 hours, then sampled the blood of groupers for analysis of cortisol, glucose, $NH_3$, GOT, GPT, total protein (TP), electrolytes ($Na^+$, $Cl^-$) and osmolality in plasma. The plasma cortisol, glucose, GOT and GPT were increased with increasing of elapsed time and ammonia concentration. In addition, the $NH_3$ and total protein in plasma were significantly increased to 12 hours exposed to ammonia, and then decreased. But, the values were significantly higher than the control. The plasma electrolytes ($Na^+$, $Cl^-$) and osmolality were decreased with increasing of elasped time. The results in the present study suggest that the continuous exposure of ammonia to juveniles of sevenband grouper may be a stressor and negative influence.