• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.205-220
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• 2005

This study was conducted to investigate the effect of the addition of glycine betaine to the diet on growth performance in pigs. A total of 400 pigs were divided into 4 feeding stages(Growing I: 23.10 $\pm$ 1.43, Growing II: 37.69$\pm$ 1.62, Finishing I: 66.51 $\pm$3.44 and Finishing II: 90.42$\pm$ 2.17 kg of initial body weight) then each feeding stage was divided into 4 treatment groups(Control: 0 0/0, Tl : 0.2 0/0, T2: 0.4% and T3 : 0.6 % of glycine betaine, respectively). The average daily gain and feed efficiency of T2 and T3 were significantly increased(p< 0.05) by dietary glycine betaine in stage I, 2 and 3. This result indicates that dietary glycine betaine could influence the pig growth performance. In feeding stage 4, the average daily gain and feed efficiency were significantly increased in 0.4% glycine betaine feeding group compared with other dietary groups(p < 0.05). Results suggest that feeding the pigs 0.4 % glycine betaine could be the most efficient dietary level. Crude protein, ether extract and crude ash digestibilities of 0.4% glycine betaine fed group were significantly increased compared with those of control group(p < 0.05). However, no significant difference was found in nutrient digestibilities among glycine betaine fed groups. Apparent faecal amino acid digestibilities of 0.4% glycine betaine fed group were more significantly higher than that of control group. The 0.4% glycine betaine fed group was significantly increased in apparent faecal amino acid digestibility compared with those of other glycine betaine fed group. No significant difference was shown in amount of microflora population between control and glycine betaine fed groups. Ammonia and hydrogen sulfide gas emission were significantly decreased in 0.4% glycine betaine feeding group compared with other dietary groups(p < 0.05).

• Journal of Animal Science and Technology
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• v.53 no.6
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• pp.533-540
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• 2011

The objectives of experiments were to compare directly the effects of synchrony of slowly fermented (corn and corn gluten meal; C-CGM) and of rapidly fermented ingredients (barley and soybean meal; B-SBM) in the rumen on starch disappearances in gastrointestinal tracts (Experiment 1) and growth performance (Experiment 2) of Hanwoo steers in the feedlot barn. In experiment 1, four Hanwoo steers ($288{\pm}21$ kg) fitted with ruminal and "T" shaped duodenal cannula were placed in one pen with Calan gate and assigned randomly to a duplicate $2{\times}2$ Latin square design. In experiment 2, eight intact Hanwoo steers ($311{\pm}8$ kg) were assigned randomly to one of two pens with Calan gate to evaluate the effect of the same diets as like in experiment 1 on growth performance. There were no differences in ruminal pH, ammonia and total VFA concentrations between treatments. Percentage of apparent ruminal starch disappearance was 33.3% unit lower (p<0.05) for steers fed C-CGM than for steers fed B-SBM diets and this difference resulted in 268% higher (p<0.05) in duodenal starch flows for steers fed C-CGM diet than for steers fed B-SMB diet. There was significant increase (p<0.05) in quantity (927 vs. 400 g/d) of corn starch digested post-ruminally compared to barley starch. However, percentage of starch apparently digested post-ruminally was 8% higher (p=0.1) in steers fed fast synchrony diet with B-SBM than in steers fed slow synchrony diets with C-CGM. The differences of percentage and amount of starch apparently digested post-ruminally between C-CGM and B-SBM diets did not affect rice straw DM intake, average daily gain (ADG) and feed efficiency. In conclusion, there is some uncertainty in regards to the relationship between site of starch digestion and DM intake, ADG, and feed efficiency in this study.

• Journal of the Korea Organic Resources Recycling Association
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• v.13 no.4
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• pp.136-147
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• 2005

Leachate from landfill sites contains high organics, chloride and ammonium nitrogen in concentration which might be potentially major pollutants to surface and groundwater environment. Most of landfill leachate treatment plants in Korea consist of biological processes to remove BOD and nitrogen. However, the efficiencies of refractory organics removal, nitrification and denitrification have not met frequently the national effluent regulation of wastewater treatment facility, especially in winter season. Simultaneous removal of organics and nitrogen from leachate is strongly necessitated to meet the national regulation on effluents from leachate treatment facilities. The intermittently aerated biological activated carbon fluidized bed(IABACFB) process was applied to treat real landfill leachates containing refractory organics and high concentration of ammonium nitrogen. The IABACFB reactor consisted of a single bed in which BAC fluidizing and an aerating column. The fluidized bed is intermittently aerated through the blower located at the aerating column. Experiments were performed to evaluate the applicability of Intermittently Aerated BACFB for simultaneous removal of refractory organic carbon and ammonium nitrogen of leachate. Organics and ammonia nitrogen($NH{_4}{^+}-N$)are oxidized during the aerobic stage, and nitrite-nitrate nitrogen($NO{_x}{^-}-N$) are removed to nitrogen gas through denitrification reaction during anoxic state. The IABACFB reactor condition reached a steady state within 40 days since the reactors had been operated. The blowing mode of 60 min.-On/60 min.-OFF is more compatible to remove TOC and ($NH{_4}{^+}-N$) operated. The blowing mode of 60 min.-On/60 min.-OFF is more compatible to remove TOC and ($NH{_4}{^+}-N$) simultaneously than the mode of 30 min.-On/90 min.-OFF. The average removal efficiencies of TOC, the refractory organic carbon, and the average efficiencies of nitrification and denitrification were 90%, 75%, 80%, 95%, respectively.

• Journal of the Korea Organic Resources Recycling Association
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• v.2 no.2
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• pp.39-51
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• 1994

When broiler litter was composted under the control of peak temperature of piles(uncontrolled, controled below $70^{\circ}C$ and below $60^{\circ}C$), changes of physical and chemical parameters were determined throughout the processing period. Broiler litter was composted in each of three $1.0{\times}1.0{\times}1.2m$ dimensional facilities for 8 weeks. After 5 to 6 weeks of composting, broiler litter was converted into the final compost with no ammonia odour, rice hull size of particle, and faint brown color. Central temperature of piles reached to the peak(about $69{\sim}70^{\circ}C$) within 3 to 4 days after composting and gradually decreased thereafter. The final product contained 26.5% of moisture, 9.0~9.1 of pH, and 14.0~14.3 of C/N ratio. The increase of C/N ratio with processing resulted from the considerable loss of N. The total wet weight of the final composts was an average of 38.3% of the initial weight, the dry weight of those 64.1%, and the organic matter weight of those 34.8%. Treatments of central temperature of composts did not affect changes of moisture, pH, C/N ratio, total wet weight, total dry weight, and total organic matter weight. In general, composted broiler litter was converted into the final product with little change in physical and chemical parameters after 5 to 6 weeks of processing. Nitrogen losses during the composting should be prevented for the improvement of the composting efficiency of broiler litter.

• Journal of the Korea Organic Resources Recycling Association
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• v.20 no.4
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• pp.97-105
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• 2012

This study examined for possibility of the food wastewater incineration treatment method as one of overland treatment method by incineration through liquefied spray of food wastewater when incinerating domestic wastes under operation and for the relationship, etc of air discharge material discharged in incineration, and the results of study are as follow: The food wastewater as one of overland treatment method was analysed 94-96% of moisture contents. Temperature of incinerator outduct during mixed incineration of food wastewater with MSW was average $897^{\circ}C$ and incineration of only MSW was $925^{\circ}C$. Temperature of the mixed incineration of food wastewater was dropped about $28^{\circ}C$ by incineration of only MSW. Concentration of nitrogen oxides(NOx) among air discharge gases was studied by 50ppm, 46ppm when inputting $200{\ell}/hr$, $300{\ell}/hr$ into the incinerator as the quantity of food wastewater. In the mixed incineration of food wastewater, generation speed of scales in the inside of a tubular exhaust gas boiler became rapid and the scale generation quantity became large but the exhaust gas boiler normally operated since scales were removed in cleaning of the tube with a compressive air cleaning facility and there was no opening clogging phenomena in a filter cloth of the filtering dust collector. The overland treatment method, not ocean dumping of food wastewater can be proposed as a technology since mixed incineration of food wastewater with MSW in the existing domestic waste incineration plant is possible, and operation costs of the incineration facility were reduced since use of chemicals such as ammonia and urinary hydrogen ion excretion, etc used in incineration facilities for removing nitrogen oxides(NOx).

• Microbiology and Biotechnology Letters
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• v.14 no.3
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• pp.225-232
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• 1986

This study was to improve the quality of Kochuzang and utilize red pepper seed. Kochuzang were prepared with the addition of red pepper seed and garlic (2％), compared the changes in the various chemical components and enzyme activity during the aging period of Kochuzang, and also organoleptic values of the products. Enzyme activities of liquefying and saccharogenic amylase, protease and lipase were increased by addition of garlic pulp and the suvival activities of enzyme except liquefying amylase were lasted high the late period of aging. Also the addition of red pepper seed was effective in maintaining the enzyme activities Change of titration acidity and pH of kochuzang were little when red pepper seed was added, but in case of a garlic additive it showed no difference at the late period of aging. Total nitrogen and amino nitrogen were increased by the addition of red pepper seed or garlic until the late period of aging, and ammonia nitrogen also increased during the middle period of againg, but showed no difference at the late period. Alcohol content was decreased by the addition of garlic or red pepper seed. but crude lipid was increased by the audition of red pepper seed. but crude lipid was increased by the addition of red pepper seed. Generally, taste, flavor and color of garlic added group were superior to the non-garlic added group for the products which aged for 10 weeks. Therefore, The quality of Barley Kochuazng may be improved by adding 2％ garlic to the whole red pepper.

• 한국해양학회지
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• v.23 no.3
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• pp.130-145
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• 1988

Spatial distribution and temporal variations of phytoplankton population were investigated in Ch$\check{o}$nsu Bay, the Korean western coast. Diurnal fluctuations of phytoplankton standing crop are associated with semidiurnal tidal cycle, as high concentration at low tide and low at high tide. In monthly variations of phytopolankton standing crop, the 1st peak occurrs in March and the 2nd one in August. The study area could be divided into two parts, outer bay and inner bay according to the physical and biological factors such as water temperature and salinity, and phytoplankton distribution patterns. The northern waters of the bay, however, may be affected by irregular fresh water influx through the lock of the dike. Because of the hydrographical differences among the surveyed stations, phytoplankton species succession patterns of each station have some differences. On the whole in this study area, Paralia sulcata and Skeletonema costatum are dominant species all the year round. However, except June, Paralia sulcata, a tychopelagic diatom is not dominant species at Station 6 (northern end of the bay). This seems to be caused by the fact that the waters of northern part of the bay is less turbulent than that of the outer bay. The result of principal component analysis (PCA) indicates that Ch$\check{o}$nsu Bay is normal coastal ecosystem where the environmental conditions are cycled in a year, and water temperature and nitrogenous nutrients such as nitrate, nitrite and ammonia are major factors to influence the annual cycle of environmental conditions.

• Journal of Oriental Neuropsychiatry
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• v.15 no.1
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• pp.211-217
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• 2004

Chronic acquired hepatocerebral degeneration(CAHD) is a heterogenous that can occur with a primary neurologic, hepatic, or combined presentation. Symptoms and signs of that included progressive dementia, dysarthria, involuntary movements(including tremor, asterixis, and choreoathetosis), ataxia of limb and gait, typically in a patient with chronic liver cirrhosis. Characteristic radiologic findings is high signal on globus pallidus on T1W1 MRI. Recently, we experienced a patients, a 73-year-old female with CAHD presenting mental change, cognitive deficits, and various involuntary movement. In our patient, T1 weighted MRI of the brain showed symmetric high signal intensity in both basal ganglia. Increased ammonia $level(226{\mu}g/dl)$ in whole blood and a multiple anomalous vessels with spleno-renal shunt on abdominal CT were found. But, liver cirrhosis is absent. In admission care, these mental change and involuntary movements had a good response to herbal medication. We report on patient with CAHD which had a spontaneous spleno-renal shunt without liver disease.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.69-76
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• 2011

This study describes the effect of myo-inositol on sustained cell division and plant regeneration from cotyledon-derived protoplast of cabbage (Brassica oleracea var. capitata). Freshly isolated protoplasts were cultured in modified Murashige and Skoog (MS) medium removed ammonia ions and containing $0.4\;mg\;l^{-1}$ thiamine HCl, $100\;mg\;l^{-1}$ myo-inositol, $2\;mgl^{-1}$ 2,4-D, $0.5\;mgl^{-1}$ BA, $30\;gl^{-1}$ sucrose and several concentrations of myo-inositol (2, 4, 6, 8, 10% (w/v)) as an osmotic stabilizer. After 3 weeks of culture in the dark at $25^{\circ}C$, the plating efficiency of cabbage protoplasts reached to $22.5{\pm}2.9%$ when cultured in modified MS medium supplemented with $2\;mgl^{-1}$ 2,4-D, $0.5\;mgl^{-1}$ BA, $30\;gl^{-1}$ sucrose and 8% (w/v) of myo-inositol at a density of $2{\times}10^5$ protoplasts/ml. Rapidly growing cell colonies after 3 weeks of culture were transferred to the same culture medium removed osmoticum. To induce shoot regeneration from calluses, calluses with about 2 mm in diameter were transferred to the MS medium containing $2\;mgl^{-1}$ BA and $0.5\;mgl^{-1}$ NAA. After further three weeks of incubation onto the medium in the light, green shoots were formed on the surface of calluses at a frequency of 30%. Upon transfer to half-strength MS basal medium, roots were formed onto the bottom of regenerated shoots without auxin treatments. These regenerated plantlets were successfully acclimatized to soil transfer, grown to normal mature plants. The cabbage protoplast culture system established in this study could be applied for production of somatic hybrids or cybrids by asymmetric protoplast fusion and mass proliferation of elite somatic clones of cabbage.

• Korean Journal of Organic Agriculture
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• v.20 no.2
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• pp.221-230
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• 2012

Rumen fermentative characteristic is useful indicators of the quality of ruminant feed stuffs and diets. An in vitro rumen fermentation experiment was therefore carried out to compare fermentation patterns among three forage sources. These were whole crop barley (WCBS), Italian ryegrass silage (IRGS) and rice straw silages (RSS). Rice straw (RS) was the control, making the treatments 4 in total. Forages were randomly allocated to serum bottles. The incubation times were arranged 0, 3, 6, 9, 12, 24, 48 and 72h at $39^{\circ}C$, respectively. Each forage source was replicated 3 times per incubation time. At each sampling time, total gas and pH were measured, whilst individual volatile fatty acids (VFAs), total volatile fatty acids (TVFAs) and ammonia nitrogen ($NH_3$-N) were determined later after storing samples at $-20^{\circ}C$. Acetate: Propionate ratio (A/P) was then calculated. Forage source had a significant effect (P<0.001) on pH and $NH_3$-N. RSS maintained higher pH values than the rest of the forage sources. A decreasing pH trend with increased time of incubation, in agreement with literature, was observed for all forage sources. WCBS recorded $NH_3$-N values higher than all the other treatments. Total gas, individual and total VFA and A/P ratio were not affected by forage source. However, there was a significant difference in all parameters (p<0.05) among forage sources at sampling periods at 3 to 72h. Therefore, the present results indicating that WCBS, IRGS, RS and RSS maintained in vitro rumen pH above the critical value. Also, WCBS produced the highest NH3-N and on this merit could be of better nutritive value, in vivo, in the ruminant.