• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.1
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• pp.19-24
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• 2009

This study was performed to investigate the antioxidative effects such as the inhibition of malondialdehyde (MDA) and bovine serum albumin (BSA) conjugation reaction, inhibition of $Fe^{2+}$-induced lipid peroxidation and the scavenging effect on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical, as well as antimutagenic capacities as Ames test in ethanol extracts of Agaricus bisporus. Agaricus bisporus ethanol extracts inhibited $Fe^{2+}$-induced lipid peroxidation and scavenged DPPH radical. The $IC_{50}$ of Agaricus bisporus ethanol extracts were 78.63 mg/assay for inhibition of MDA with BSA conjugation reaction, 4.06 mg/ assay for inhibition of $Fe^{2+}$-induced lipid peroxidation and 1.08 mg/assay for scavenging effect on DPPH radical. So, among the methods used in this study, the most effective antioxidative capacity in ethanol extracts of Agaricus bisporus was the scavenging effect on DPPH radical. The indirect and direct antimutagenic effects of ethanol extracts of Agaricus bisporus were examined by Ames test using Salmonella Typhimurium TA98 and TA100. The inhibitory effects on direct mutagenicity mediated by sodium azide in Salmonella Typhimurium TA100 and 2-nitrofluorene in Salmonella Typhimurium TA98 were 100%. The inhibition rates on indirect mutagenicity mediated by 2-anthramine were 86.09% in the Salmonella Typhimurium TA98 and 81.93% in the Salmonella Typhimurium TA100. The ethanol extracts of Agaricus bisporus showed considerable antioxidative activity and strong antimutagenic capacity.

• Journal of Food Hygiene and Safety
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• v.25 no.3
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• pp.215-219
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• 2010

This study was undertaken to investigate the mutagenicity and antimutagenicity of Acanthopanax koreanum Nakai. Antimutagenic study on extract of A. koreanum was studied using the test with Salmonella typhimurium TA100, TA98. And mutagenicity study was studied using the test with S. typhimurium TA100, TA98, TA1535, TA1537 and Escherichia coli WP2 uvr A. A. koreanum was negative in Ames test with S. typhimurium and E. coli with or without S-9 mixture. Test substances of $5000\;{\mu}g/{\mu}l$, $2500\;{\mu}g/{\mu}l$ and $600\;{\mu}g/{\mu}l$ of A. koreanum extracts were chosen via toxicity test. Ames test was performed on positive control group, experimental group and negative control group in the presence of the metabolic activation system and metabolic non-activation system. As a result, there was no coherent increase and reverse mutation in all concentrations. Therefore, A. koreanum does not cause reverse mutation. In addition, A. koreanum showed strong antimutagenic activities in S. typhimurim TA100 and TA98. In conclusion, A. koreanum root may be an excellent antimutagenic agent.

• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1426-1432
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• 2000

The effects of raw anchovy, salted raw anchovy (20% salt+anchovy), 6- and 12-month fermented anchovy (20% salt added) on the N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutagenicities were evaluated using Salmonella assay and the SOS chromotest. The methanol extracts from raw, salted and the fermented anchovy (FA) sample increased the revertants of Salmonella typhimurium TA100 at the level of $0.125{\sim}5\;mg/plate$ in Ames test. The salted raw anchovy extract induced the largest number of the revertants. All the FA extracts had comutagenic effect on the MNNG. Twelve-month FA juice exerted the lowest comutagenic activity among the FA samples. The comutagenicity of 12-month FA was due to the synergistic effect of salt and histidine which teem in FA. Thus the Ames test using histidine requiring mutant, S. typhimurium, is not appropriate to determine the mutagenicity of FA which is rich in histidine. In SOS chromotest using E coli, raw, salted and fermented anchovy extracts did not show any mutagenicity in the absence of MNNG. The raw and fermented anchovy samples blocked the SOS response of E. coli PQ37 induced by MNNG, while raw salted anchovy increased the SOS induction factor. Twelve-month FA juice showed higher antimutagenic effects than 6-month FA samples (both solid and liquid). The ripened (12-month) FA along with raw anchovy in the SOS chromotest exhibited antimutagenic activity.

• The Korean Journal of Physiology
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• v.25 no.2
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• pp.101-113
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• 1991

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.9 no.6
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• pp.19-25
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• 1988

• 한국전산유체공학회:학술대회논문집
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• 2007.04a
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• pp.1-1
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• 2007

• Journal of Food Hygiene and Safety
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• v.10 no.1
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• pp.29-32
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• 1995

Inclusion complex of omeprazol with $\beta$-Cyclodextrin and Hydroxypropyl-$\beta$-Cyclodextrin were prepared by coprecipitation and freeze-drying method respectively. Effects of these inclusion complex on RBCs were monitored with a spectrophotometer by the method of Kahan et al. and the mutagenic activity based on the Ames plate incorporation test in the presence and absence of liver microsomal enzyme(S9 fraction) using Salmonella typhimurium TA98 and TA100. The RBCs hemolysis and mutagenic activity of these complex were not detected.

• Journal of Food Hygiene and Safety
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• v.20 no.1
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• pp.18-21
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• 2005

These observations were performed to investigate the safety of the natural herbs (Rhus-II) in respect of genotoxicity. This substance was examined in two in-vitro tests: (1) Salmonella typhimurium reversion assay (Ames test) in strain TA 98, TA 100, TA 1535 and TA 1537, (2) in vitro chromosome aberration test in cultured Chinese hamster ovary (CHO) cells. In the reverse mutation test, Rhus-II did not induced mutagenicity in Salmonella typhimurium reversion assay(Ames test) with or without metabolic activation. In the chromosome aberration assay using CHO cells, there was no increased incidence of structural and numerical aberrations with or without metabolic activation. These results indicated that, the Rhus-II had no genotoxicity.

• KSBB Journal
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• v.4 no.1
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• pp.8-10
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• 1989

Mutagenic characteristics deactivation of 7, 12-dimethylbenz(a)anthraracene was studied in a nonacclimated sandy loam soil at low and neutral pH soil conditions. Soil extracts containing transformation products were separated into three fractions based on HPLC retention time(polarity). Highly polar transformation products of 7, 12-dimethylbenz(a)anthracene demonstrated a negative mutagenic response with the Ames mutagenicity assay, strain TA-100, for both low and neutral pH soils. Moderate and low polar fractions, however, induced mutagenicity for both soil samples with mutagenic ratios similar to those of the parent compound.

• The Korean Journal of Food And Nutrition
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• v.14 no.4
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• pp.329-332
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• 2001

The antimutagenic activity of resveratrol on the mutagenicity induced by Trp P-1 (3-amino-1,4-dime-thyl-5H-pyrido{4,3-b}indole) was studied using the Ames test with Salmonella typhimurium TA98 and 100. Trp P-1 showed strong mutagenecity in S. typhimurium TA98, but was higly decreased mutagenecity in S. typhimurium TA100. This result suggests that the mutagenecity of Trp P-1 can be mainly induced by the DNA lesions causing frame shift. Resveratrol itself did not show antibacterial effect upon 300 $\mu\textrm{g}$/assay. Resveratrol showed the strongest inhibitory effect with dose dependent manner on the mutagenicity induced by Trp P-1. The inhibition rates of resveratrol at concentration of 2, 10, 25, 50, 100, 300 $\mu\textrm{g}$/assay were 13%, 37%, 52%, 65%, 81%, 89%, respectively.