• Childhood Kidney Diseases
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• 제23권2호
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• pp.93-99
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• 2019

C3 glomerulopathy is a renal disorder involving dysregulation of alternative pathway complement activation. In most instances, a membranoproliferative pattern of glomerular injury with a prevalence of C3 deposition is observed by immunofluorescence microscopy. Dense deposit disease (DDD) and C3 glomerulonephritis (C3GN) are subclasses of C3 glomerulopathy that are distinguishable by electron microscopy. Highly electron-dense transformation of glomerular basement membrane is characteristic of DDD. C3GN should be differentiated from post-infectious glomerulonephritis and other immune complex-mediated glomerulonephritides showing C3 deposits.

• Animal Bioscience
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• 제36권2호
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• pp.315-321
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• 2023

Objective: The study was conducted to investigate the dephosphorylation of Pseudomonas aeruginosa flagellin (PA FLA) by sweet potato purple acid phosphatase (PAP) and the effect of the enzyme on the flagellin-mediated inflammatory response in the A549 lung epithelial cell line. Methods: The activity of sweet potato PAP on PA FLA was assayed at different pH (4, 5.5, 7, and 7.5) and temperature (25℃, 37℃, and 55℃) conditions. The release of interleukin-8 (IL-8) and the activation of nuclear factor kappa- light-chain-enhancer of activated B cells (NF-κB) in A549 cells exposed to PA FLA treated with or without sweet potato PAP was measured using IL-8 and NF-κB ELISA kits, respectively. The activation of toll-like receptor 5 (TLR5) in TLR5-overexpressing HEK-293 cells exposed to PA FLA treated with or without sweet potato PAP was determined by the secreted alkaline phosphatase-based assay. Results: The dephosphorylation of PA FLA by sweet potato PAP was favorable at pH 4 and 5.5 and highest at 55℃. PA-FLA treated with the enzyme decreased IL-8 release from A549 cells to about 3.5-fold compared to intact PA FLA at 1,000 ng/mL of substrate. Moreover, PA-FLA dephosphorylated by the enzyme repressed the activation of NF-κB in the cells compared to intact PA FLA. The activation of TLR5 by PA-FLA was highest in TLR-overexpressing HEK293 cells at a substrate concentration of 5,000 ng/mL, whereas PA FLA treated with the enzyme strongly repressed the activation of TLR5. Conclusion: Sweet potato PAP has the potential to be a new alternative agent against the increased antibiotic resistance of P. aeruginosa and may be a new conceptual feed additive to control unwanted inflammatory responses caused by bacterial infections in animal husbandry.

• 한국중재학회지:중재연구
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• 제20권3호
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• pp.71-91
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• 2010

ADR is alternative dispute resolution that includes mediation, adjudication, arbitration, conciliation and ombudsman schemes. ADR may be an alternative to going to court or to a tribunal. The main types of ADR are conciliation, arbitration or mediation and ADR is divided into national leading ADR and private lading ADR and national leading ADR includes court-annexed ADR and administrative ADR. Court-annexed ADR has become a well established feature of the judicial systems on a global basis. The bulk of court-annexed ADR in Glove is by way of mediation. Thus each nation takes part in ADR by court involvement and Enactment of ADR-related Laws. And the involvement of nations have both the regulative character and promotive character in ADR. In addition to the national leading ADR, the private leading ADR also must be activated as United Kingdom. Thus this paper deals with national leading ADR and private leading ADR and the purpose of this paper is to contribute to the activation of ADR by studying the promotion and limited the involvement of nation in ADR and private leading ADR in United Kingdom.

• 농촌계획
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• 제19권2호
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• pp.149-162
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• 2013

This study intended to suggest Voluntourism as a solution for alternative rural tourism for regional activation. Voluntourism is a form of alternative tourism on values of variety of tourism demand from pleasure to virtue. This study analyzed actual state of WWOOF(World-Wide Opportunities on Organic Farms) activities being operated in Korean rural village, one of representative of Voluntourism. Study aimed to explore the possibility of WWOOF activity as one of model of alternative rural tourism. We apply grounded theory method one of qualitative study methods for analysis of WWOOF Activities because WWOOF is still small activity in Korea. In result, we figure out the actual condition of WWOOF Korea activities as a vouluntourism through paradigm model of host and guest who had experience in WWOOF Korea. WWOOF activity is in its early stage so there many tasks. We suggested ideas for improving WWOOF activities to be one of new rural tourism business.

• 방송공학회논문지
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• 제13권5호
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• pp.752-759
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• 2008

최근 방송과 통신의 융합은 미디어와 미디어 사이의 영역의 한계를 모호하게 하고 있다. 매체의 진화에 따른 디지털 기술의 발전에 따라 사용자는 상호작용성을 경험하였다. 특히 불특정 다수의 참가자가 실시간 환경 속에서 동일한 목표를 가지고 몰입 하거나 경쟁 하면서 만족을 추구하는 MMORPG(Massively Multi-player On line Role Play Game)는 사용자에게 새로운 형태의 문화와 만족감을 주고 있다. ARG(Alternative Reality Game)는 미디어의 융합에 의해 새로이 탄생한 대체현실게임으로, ARS, SMS, BLOG, Cafe, Message 등을 통해 사용자와 사용자간에 보다 더 현실적인 상호작용을 수행한다. 본 논문에서는 ARG를 소개하고, ARG의 구현, 설계, 구축의 방법을 인간의 사고와 미디어 측면에서 분석하였으며, ARG 활성화를 통해 방송의 새로운 방향을 제언한다.

• Childhood Kidney Diseases
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• 제23권1호
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• pp.43-47
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• 2019

Atypical hemolytic uremic syndrome (aHUS), a rare form of thrombotic microangiopathy, is distinguished from the typical form by the absence of a preceding verotoxin-producing Escherichia coli infection. Notably, aHUS occurs in association with genetic or acquired disorders causing dysregulation of the alternative complement pathway. Patients with aHUS may show the presence of anti-complement factor H (CFH) autoantibodies. This acquired form of aHUS (antiCFH-aHUS) primarily affects children aged 9-13 years. We report a case of a 13-year-old Lao girl with clinical features of aHUS (most likely anti-CFH-aHUS). The initial presentation of the patient met the classical clinical triad of thrombotic microangiopathy (microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury) without preceding diarrheal illness. Low serum levels of complement 3 and normal levels of complement 4 indicated abnormal activation of the alternative complement pathway. Plasma infusion and high-dose corticosteroid therapy resulted in improvement of the renal function and hematological profile, although the patient subsequently died of infectious complications. This is the first case report that describes aHUS (possibly anti-CFH-aHUS) in Laos.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.47.1-47
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• 2001

The drilling process has a great importance for the production technology due to its widerspread use in the manufacturing industry. In order to enhance a maximum production rate and prevent the drill from the damage, it is important to monitor and control the drilling system. Thrust force and cutting torque are the main output variables in the design of drilling control systems. In this paper, an alternative estimation method of thrust force by using flexible neural networks is proposed. Flexible neural network uses the sigmoid activation function with adjustable parameter in order to enhance the approximation accuracy ...

• 한국식품영양과학회지
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• 제26권2호
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• pp.248-253
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• 1997

계지에서 분리한 보체계 활성화 다당의 보체 활성화양식과 다당의 당쇄결합에 관하여 검토하였다. 계지 보체계 활성화 다당획분들(CC-IIIa, CC-IIIb, CC-IIIc)들은 정상인의 혈청중에 존재하는 주요 보체성분인 C3를 활성화하여 C3a와 C3b 보체 산물을 생성 시켰는데 이 과정은 anti-human C3를 이용한 면역전기영동을 실시하여 확인하였다. $Ca^{++}$ 유무시의 보체계 활성을 측정한 결과 계지에서 분리된 CC-IIIa-3는 고전적 경로(classical pathway)와 부경로(alternative pathway)를 동시에 활성화시킴을 확인하였다. CC-IIIa-3을 메틸화하여 GC/MS로 분석한 결과 비환원 말단의 galactopyranose와 glucopyranose가 확인되었고 1,2-linked rhamnopyranose, 1,6-linked mannopyranose, 1,3-linked glucopyranose, 1,6-linked galactopyranose 등이 확인되었다.

• 한국중재학회지:중재연구
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• 제21권1호
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• pp.87-107
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• 2011

There are two methods to resolve conflicts related to constructions which are through lawsuits and ADR (Alternative Dispute Resolution) just like any other conflicts. Along with the special characteristics pertaining to the conflicts related to constructions, the advantages that ADR is in possession of such as its cost, duration and professionalism, resolving conflicts through ADR has been considered to be more logical than any other options recently. In Korea's case at present, the resolution of conflicts regarding constructions through ADR is mostly dependent on administrative mediation or through arbitration. However, in the case of the administrative mediation, its usage rate is very low due to problems caused by problems in its running and effectiveness. In the case of arbitration, the services of the Korean Commercial Arbitration Board is comparatively used more but because of the fact that arbitration relies on a single trial system and the fact that its executive powers while having the same effectiveness as the final ruling does not get acknowledged leads to the phenomena of avoiding its usage. In addition, in relation to the selective arbitration clause, the problem of effectiveness of the arbitrative agreement is becoming a hindrance to the activation and promotion of the arbitration process. Furthermore, in the case where the ordering body is the government, the public servant involved in the case avoiding the arbitration process because of concerns of being penalized by the internal and external audit within the institution is becoming a problem as well. These problems are not only limited to conflicts regarding constructions and there needs to be actions taken to promote the activation of ADR by enacting a basic law. The more important issue at hand however is offering a resolution measure that would be the most appropriate for users and this could probably be done only through actions such as implementing the American partnering system or the dispute adjudication board system so that they can supervise the resolution of conflicts through mediation, arbitration, and assistance as well as offering consultations regarding conflicts related to constructions.

• 생약학회지
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• 제44권2호
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• pp.110-117
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• 2013

Cirsium japonicum (CJ) leaf (L) alcoholic extracts were investigated for analysis their active components (flavonoids and flavanolignans; silymarins) and inhibitory effect on transforming growth factor (TGF)-${\beta}$ induced hepatic stellate cells (HSCs, LX-2 cells) activation. The CJ root (R) extracts were also analyzed and compared with leaf extracts. Total flavonoid and polyphenol contents of the leaf extracts showed higher than those of the root extracts. The content of each flavonoid compound, which was analyzed by HPLC, in CJ-L extracts was also higher than in CJ-R extracts. The results of flavanolignans content in CJ-L and CJ-R extracts were consistent in flavonoid and polyphenol. We studied inhibitory effect of two extracts against TGF-${\beta}1$ induced HSCs activation. The CJ-L extracts significantly suppressed overexpression of profibrogenic factor, ${\alpha}$-smooth muscle actin and collagen-${\alpha}1$(I). The CJ-R extract also showed inhibitory effect on TGF-${\beta}1$ induced HSCs activation, but the efficacy was lower than in CJ-L extract. These results suggest that CJ-L may contribute to the fibrotic liver treatment.