• Environmental Analysis Health and Toxicology
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• 제19권2호
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• pp.153-159
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• 2004

To ascertain the effects of hair dyeing application on oxidative stress in human, a mixture of permanent black colored hair dye with the same amount of oxidant containing 6% hydrogen peroxide was used. A hair dyeing with contacting the scalp (conventional dyeing) and a hair dyeing with 3 to 4mm away from the scalp (alternative dyeing) were applied to each 15 young healthy women. Blood was taken from the brachial vein at two sampling times, just before and 6 hours alter the hair dyeing, and antioxidant enzyme activities and antioxidant contents were measured in red blood cells. After dyeing, malondialdehyde(MDA) contents for conventional dyeing group was shown to a tendence of more increased than alternative dyeing group. After dyeing, reduced glutathione (GSH) contents for conventional dyeing group was shown to a tendence of more decreased than alternative dyeing group. After dyeing, superoxide dismutase (SOD) and catalase (CAT) activities were significantly decreased in conventional dyeing group (p < 0.01), however, SOD and CAT activities were not significantly decreased in alternative dyeing group. After dyeing, there was no significant decrease in glutathione peroxidase (GPX) activity both for conventional dyeing group and alternative dyeing group. Therefore, after dyeing, the degree of oxidative stress in red blood cells for alternative dyeing group was appeared to be lower than conventional dyeing group.

• 생약학회지
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• 제41권4호
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• pp.255-263
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• 2010

Kojongsi persimmon (Diospyros kaki) is the major cultivar of astringent persimmon in southern of Korea. Kojongsi persimmon leaf has been traditionally used for acute and chronic diseases in Oriental countries. The purpose of this study was undertaken to investigate the antioxidative activities of the extract of Kojongsi persimmon leaf during growth. We investigated the antioxidant effects of the persimmon leaf extracts during growth on total polyphenol, total flavonoid, electronic donating ability (DPPH), nitrite (NO) scavenging and superoxide dismutase (SOD)-like activity. The next, we investigated the possible cell protective effects of the persimmon extract treatment against ultraviolet B (UVB)-induced injury in HaCaT keratinocytes. The contents of total polyphenol and flavonoid in leaf extract of Kojongsi persimmon were increased in time-dependent manner. In Jun, DPPH and NO radical scavenging and SOD-like activities in the leaf extract of Kojongsi persimmon was increased to the highest. However, the antioxidant activities in persimmon varieties were not any difference. The cell cytotoxicity by UVB irradation in HaCaT keratinocytes was significantly increased with the compared to the control group. However, the treatment of leaf extract of Kojongsi persimmon in HaCaT keratinocytes was shown to protective effect against UVB-induced cell cytotoxicity. These results suggest that the leaf extract of Kojongsi persimmon has potent antioxidant activity, and protective effect against UVB-induced keratinocyte injury. Thus, these properties may be contributed in the care of acute and chronic diseases.

• 농촌계획
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• 제19권2호
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• pp.149-162
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• 2013

This study intended to suggest Voluntourism as a solution for alternative rural tourism for regional activation. Voluntourism is a form of alternative tourism on values of variety of tourism demand from pleasure to virtue. This study analyzed actual state of WWOOF(World-Wide Opportunities on Organic Farms) activities being operated in Korean rural village, one of representative of Voluntourism. Study aimed to explore the possibility of WWOOF activity as one of model of alternative rural tourism. We apply grounded theory method one of qualitative study methods for analysis of WWOOF Activities because WWOOF is still small activity in Korea. In result, we figure out the actual condition of WWOOF Korea activities as a vouluntourism through paradigm model of host and guest who had experience in WWOOF Korea. WWOOF activity is in its early stage so there many tasks. We suggested ideas for improving WWOOF activities to be one of new rural tourism business.

• Food Science and Biotechnology
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• 제18권4호
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• pp.1042-1045
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• 2009

A commercial propolis was investigated in terms of its antioxidant, antimicrobial, and antiproliferative activities. The contents of total phenol and flavonoid of propolis were 8.3 and 6.6 mg, respectively. The reducing power of the propolis increased with concentration increasing. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was shown at 82.70% in 1,000 ${\mu}g/mL$ of the propolis. 2,2'-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging effect of antioxidant activity on the propolis was 35.64 g/sample. The propolis showed high antimicrobial activity against Bacillus cereus at all concentration of propolis. All of the cancer cell lines have 53-73% as effective growth inhibition. These results showed that the commercial propolis has potential antioxidant, antimicrobial, and cancer cell proliferative inhibition activities thus, propolis can be applied to the functional food, pharmaceutical, and cosmetic industry.

• 복식
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• 제63권8호
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• pp.143-155
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• 2013

The outdoor apparel market has been growing very fast recently, and consumers are spending more time doing outdoor activities for their leisure. As the outdoor apparel market is growing, it has started to combine functionality with style to provide more diverse designs and colors for all ages. The increase of interest in heathy life-styles, outdoor activities, and leisure has lead to a rise in demand for outdoor apparel. Now outdoor apparel have become a part of people's daily casual wear in addition to being worn for outdoor activities. This study is focused on a outdoor jacket design which is used as down-alternative material and the purpose of this study is to design outdoor jackets using a down-alternative fabrics that improve the thermal and lightweight effects for clothing comfort, and increase the satisfaction of wearing down-alterative outdoor jacket. The research of this paper was done by using a questionnaire survey. The survey was used to examine the male customers' outdoor activities, purchase behaviors, place, expense and design preferences. Then, down-like fabric was used to make the prototype down jacket and its design, function, and satisfaction with wearing compared with other brands down jacket products were analyzed. Although duck down fabrics are representative of outdoor jackets, they have many problems that need to be solved such as feathers falling out or its vulnerability to moisture. They have competitive prices compared to down jackets so down-alternative jackets are able to gain more proportion of its market share.

• 한국초등과학교육학회지:초등과학교육
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• 제26권2호
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• pp.171-180
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• 2007

This study analyzed the effect of Human-oriented Alternative Science Activities(HASA) on a coaching teacher and the students who participated in this activity. Human-oriented alternative science activities were used in the experimental group and traditional teaching methods were used in the control group. In pretest and posttest sessions, both groups were evaluated using an academic achievement test, and an affective characteristics related science test. After the activity, questionaries regarding attitudes to science lessons were administered as well. The process of change (if any) which occurred in the science lessons of the experimental group teacher was also analyzed through the teacher's diaries. The results showed that application of HASA had a positive effect upon affective characteristics and attitudes to science classes held by the experimental group and teacher. However, the result of this study also showed that the variation between academic achievement of each group was statistically insignificant.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2006년도 춘계학술대회
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• pp.42-44
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• 2006

• 동의생리병리학회지
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• 제26권1호
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• pp.59-66
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• 2012

Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, including atopic dermatitis. The present study was undertaken to investigate the antioxidative and anti-inflammatory activities of the extract of Duchesnea chrysantha (DCE). DEC was prepared by extracting with 80% ethanol. Total flavonoids and polyphenols were measured by a colorimetric assay. The free radical scavenging activity of the extract was analyzed by the DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and Griess reagent assay. An oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The level of prostaglandin $E_2$ ($PGE_2$) was measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were measured by Western blot analysis. Total flavonoid and polyphenol contents of DCE were included $24.73{\pm}0.45$ and $178.77{\pm}2.65$, respectively. DCE significantly increased electron donating ability (DPPH), nitrite scavenging (NO) and ABTS reducing activity in dose dependant. We investigated the anti-inflammatory effects of DCE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. DCE significantly suppressed NO and prdstaglandin $E_2$ ($PGE_2$) in dose dependant. Furthermore, the levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with DCE in a dose dependent manner. These results suggest that DEC may has value as natural product with its high quality functional components, antioxidative and anti-inflammatory activities.

• 한국균학회지
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• 제43권4호
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• pp.207-215
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• 2015

Nanotechnology, which has become an important area of science, has caused an enormous developmental revolution in many fields. In the last two decades researchers have focused on overcoming the obstacles encountered during the preparation of nanoparticles. This article highlights the nanotechnology, along with a brief description of the manufacturing, concepts and activities of nanoparticles as alternative pesticides against plant pathogens, some methods for evaluation of nanoparticles against phytopathogens in vitro and in vivo, and explains the importance of some common nanoparticle types used in agricultural applications and plant pathology.

• Journal of Microbiology and Biotechnology
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• 제34권8호
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• pp.1642-1652
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• 2024

Arctium lappa (Burdock) root is used in various culinary applications especially in Asian Cuisine. Arctigenin (ARC) is a polyphenolic compound abundant in the roots of the burdock plant from which it derives its name. The emergence of bacterial resistance is a growing global worry, specifically due to the declining availability of new antibiotics. Screening for the antibacterial candidates among the safe natural products is a promising approach. The present study was aimed to assess the antibacterial activity of ARC against Pseudomonas aeruginosa exploring its effect on the bacterial cell membrane. Furthermore, the anti-virulence activities and anti-quorum sensing (QS) activities of ARC were in vitro, in vivo and in silico assessed against P. aeruginosa. The current results showed the ARC antibacterial activity was owed to its disruption effect of the cell membrane. ARC at sub-MIC significantly decreased the formation of biofilm, motility, production of extracellular enzymes and in vivo protected mice against P. aeruginosa. These anti-virulence activities of ARC are owed to its interference with bacterial QS and its expression. Furthermore, ARC showed mild effect on mammalian erythrocytes, low probability to induce resistance and synergistically combined with antibiotics. In summary, the promising anti-virulence properties of ARC indicate its potential as an effective supplement to conventional antibiotics for treating severe P. aeruginosa infections.