• Asian-Australasian Journal of Animal Sciences
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• 제33권5호
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• pp.753-762
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• 2020

Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.

• 건축역사연구
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• 제23권6호
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• pp.7-20
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• 2014

The purpose of this paper is to study on the architecture and extension project for the Church of St. Lorenzo. This study is composed of a through analysis of the extension elements on the church building and extension type for extension project of the Church of St. Lorenzo. The results of study are as follows: 1. The extension project for the Church of St. Lorenzo was basically consisted of the concept of a horizontal extension plan through both the interior and exterior space of the church building. This project was the plan of concept of additional or affixing extension through existing spaces with necessary spaces to make up for the previous simple space and form as well as functional aspect. 2. The unit spaces of the nave, the aisle, the small chapels bilaterally adorning the aisles, the transept and the chapels around transepts, and the sacristies by the extension project for the Church of St. Lorenzo is composed of regulation, balance and harmony as geometric space composition according to simple proportion system on whole and parts as well as parts and parts, through the definite articulation by each space zoning. 3. The most important innovative aspect different to previous churches in the extension project for the Church of St. Lorenzo is the compositive system of each other symmetrical spaces through long centrical axis of central part, applying the module system for horizontal arrangement. In particular, the use of regular module on repetition and proportion of rhythm from architectural composition system was precisely composed with the alter that consists of visual focus to express more accurate perspective.

• 폴리머
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• 제38권3호
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• pp.314-319
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• 2014

힌더드 아민기를 포함하고 있는 헤테로환을 가지며 알킬사슬 길이가 서로 다른 세 종류의 나일론 4 열안정제를 합성하였다. 힌더드 아민기를 이용하여 라디칼에 의한 열분해를 방지하고, 열안정제와 나일론 4의 아마이드기 간 수소결합을 조절하여 나일론 4의 열적 특성에 미치는 영향을 확인하였다. 안정제의 알킬사슬의 수가 4개일 때 수소결합이 가장 최적화되는 것으로 등온 TGA 결과를 통해서 확인하였다. 또한 시판중인 나일론 6의 열안정제를 사용하여 나일론 4에 대한 열안정성을 비교 실험한 결과, 합성 열안정제가 나일론 4의 열안정성을 실질적으로 향상시킨 것을 확인하였다.

• 정보처리학회논문지C
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• 제10C권6호
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• pp.675-682
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• 2003

공격자는 시스템에 침입하기 위해 시스템 취약점을 수집한 후, 여러 공격 방법을 통해 루트권한을 획득하여 시스템 정보를 유출 및 변조하며 더 나아가선느 시스템을 파괴한다. 이러한 공격에 대응하기 위해 침입 탐지 및 차단을 위한 보안 시스템들이 많이 개발디어 왔지만, 최근 공격자들은 보안 시스템들을 우회하여 시스템에 침입하기 때문에 많은 문제가 되고 있다. 본 논문에서는 루트권한을 획득한 공격자의 불법행위를 막기 위한 보안커널모듈을 제안한다. 보안커널모듈은 추가적인 패스워드를 통해 시스템의 관리자 인증을 강화하여, 공격자가 중요 파일을 변조하고 루트킷을 설치하는 행위를 막는다. 또한 공격자의 불법 행위에 대한 경고메일을 관리자에게 실시간으로 보내서, 관리자가 메일에 포함된 정보를 통해 새로운 보안 정책을 수립하도록 한다.

• 대한화장품학회지
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• 제17권1호
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• pp.64-80
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• 1991

The SKINTEX Method is based on a two-compartment physico-chemical model which includes a Biomembrane Barrier in compartment one and an organized macromolecular matrix in compartment two. Test samples absorb onto or permeate through the keratin/collagen Biomembrane Barrier and then can interact with the organized macromolecular matrix. Changes in the integrity of the barrier release a dye indicator: Changes in the matrix can alter its transparency. The sum of these two responses is read spectrophotometrically at 470nm. An early investigation of 950 chemicals and formulations in the SKINTEX System produced results which were 89% concordance to in vivo Draize dermal irritation results obtained with 24-hour occluded application of test samples with-out abrasion and standard scoring. Alkaline materials were analyzed in a specialized SKINTEX AMA Protocol. In this early study, the model did not distinguish nonirritant test materials and formulation with PDII(Primary Dermal Irritation Index)in the range from 0 to 1.2, A High Sensitivity Assay Protocol(HSA)was developed to amplify the changes in both compartments of this model and provide more accurate calibration of these changes. A study of 60 low irritation test samples including cosmetics, household products, chemicals and petro-chemicals distinguished nonirritants with PDII $\leq$ 0.7 for 26 of 30 nonirritants. A second protocol was developed to evaluate the SKINTEX model predictability with respect to human irritation. The Human Response Assay (HRA )has been optimized based on differences in penetration and irritation responses in humans and rabbits. An additional 32 test materials with different mechanisms and degrees of dermal toxicity were evaluated by the HRA. These in vitro results were 86% concordant to human patch test results. In order to further evaluate this model, a Standard Chemical Labelling (SCL) Protocol was developed to optimize this system to predict Draize dermal irritation results after a 4-hour application of the test material. In a study of 52 chemicals including acids, bases, solvents, salts, surfactants and preservatives, the SCL results demonstrated 85% concordance to Draize results for a 4-hour application of test samples on non-abraded rabbit skin. The SKINTEX System, including three specialized protocols, provided results which demonstrated good correlation to the endpoint of dermal irritation in man and rabbits at different application times.

• Toxicological Research
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• 제20권3호
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• pp.251-261
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• 2004

A large number of chemical pollutants including phthalates, alkylphenolic compounds and organochlorine pesticides have the ability to disrupt endocrine function in animals, and alter cog-nitive function. Because hormone mediated events play an important role in central nervous system development and function, the changes in cognitive function seem to be mediated by the endocrine-like action of these chemicals. The present study therefore was designed to investigate effect of bisphenol A (BPA), an endocrine disrupting chemical on neuro-behavial patterns, and expression of estrogen receptors and tyrosine hydroxylase, a limiting enzyme of dopamine synthesis pathway. BPA was treated orally for 3 weeks into 3 week old mice, and then the neuro-behavial patterns (stereo-type behaviors such as jumping rearing and forepaw tremor, climbing behavior, tail flick, rotarod and locomotor activity), and the expression of estrogen receptors and tyrosine hydroxylase were deter-mined every 3 week for 9 weeks. During the treatment of BPA, the food uptake and body weight increase were not significantly changed. BPA resulted in the increased stereotype behaviors (jump-ing, rearing and forepaw tremor) 6 or 9 weeks after treatment. The time response to tail flick and locomotor activity were decreased by the treatment of BPA, whereas the time for rotarod was increased by the treatment of BPA. The expression of estrogen receptor alpha and beta was increased in the brain and pituitary gland. Maximum expression was found in the brain after 9 week of 100 mg/kg BPA treatment and in the pituitary gland after 6 week of 100 mg/kg BPA treatment. Tyrosine hydroxylase was increased in dose and time dependent manners in the brain but no change was found in the pituitary gland. The present data show that exposure of BPA in the young mice could alter expression of estrogen receptors and dopamine synthesis pathway, thereby modulate neuro-behavial patterns (increase of stereotype behaviors but decrease locomotor activity).

• Asian-Australasian Journal of Animal Sciences
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• 제10권2호
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• pp.233-239
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• 1997

A mammary epithelial cell line (MAC-T) established as a model for lactation was utilized to identify and characterize effects of various hormones upon insulin-like growth factor binding protein secretion. Ligand and immunoblot analyses of conditioned media indicated that insulin-like growth factor binding protein-2 was secreted by MAC-T cells. Insulin-like growth factor-I stimulated insulin-like growth factor binding protein-2 secretion in a dose-dependent manner, but prolactin and bovine somatotropin did not alter insulin-like growth factor binding protein-2 secretion. Insulin increased and cortisol decreased insulin-like growth factor binding protein-2 secretion. Effects of insulin-like growth factor-I on insulin-like growth factor binding protein-2 secretion support previous studies using primary cultures of bovine mammary cells and bovine fibroblasts. Effects of cortisol and insulin on insulin-like growth factor binding protein-2 secretion may be explained by changes in protein synthesis. In addition, supraphysiological doses of insulin can cross-react with the insulin-like growth factor-I receptor and stimulate insulin-like growth factor binding protein-2 secretion. MAC-T cells provide a model system to study mechanisms that regulate local insulin-like growth factor-I bioactivity.

• 경영과정보연구
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• 제17권
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• pp.289-313
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• 2005

Electronic commerce is giving rise to many new and innovative 'gest business practices,' such as telecommuting and the virtual workplace. Telecommuting and the virtual workplace go hand in hand. Today, more then 35 million people in the United States telecommute, and that expected to grow by 20 percent over the next several years. And more then 30 million people used internet shopping mall every years in Korea. Therefore, using the internet and information technology, electronic commerce has the potential to propel a company to 'break out' of existing strategic constraints and radically alter business processes, strengthen customer and supplier ties, and open up new markets. The object of this study is the investigation method for activity solution of electronic commerce of fashion cloth shopping mall in korea. The study has investigated 2 cases about 'halfclub.com' in Korea and 'bluefly.com' in America in the research. Therefore, the study founded the 3problems of electronic commerce at fashion cloth shopping mall in korea and suggested that the activation policies of electronic commerce. The result of suggested that the new electronic commerce style, a lot of contents affects positive effects on the performance of fashion cloth shopping mall in Korea and hope that will be expect new role of electronic commerce to be the herb in fashion cloth shopping mall in korea.

• 한국컴퓨터정보학회논문지
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• 제4권1호
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• pp.85-91
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• 1999

일선 학교 조직의 복잡성이 증대됨에 따라 업무 또한 세분화, 복잡화되어 가고 있다. 이에 교사가 맡아야 하는 업무가 가중되어 학생 지도를 위한 교재 연구보다 오히려 업무 해결에 더 많은 시간을 할애해야 할 형편이다. 그 중 성적 처리 업무는 교육개혁의 일환으로 수행평가를 도입하여 실시함으로써 교육과정의 평가로 교사의 업무 부담이 더욱 가중되고 있는 실정이며 이를 해결하기 위해 컴퓨터에 대한 의존이 증가되고 있다. 이에 따라 본 논문에서는 일반적으로 사용하고 있는 (equation ommited)글 97과 한글 어프로치 97을 이용하여, 작성이 간단하고 개성 있는 양식을 직접 만들고 수정할 수 있는 성적 업무 처리 자동화 방법을 제시하고자 한다.

• Biomolecules & Therapeutics
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• 제7권4호
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• pp.390-396
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• 1999

CJ-50005 is a hepatitis A vaccine which is prepared by formalin inactivation of the HM175 virus cultured in human diploid MRC-5 cells. The general pharmacological properties of CJ-50005 were evaluated in various animals and in vitor system. CJ-50005 at the doses of 0.025, 0.25 and 0.75 $\mu\textrm{g}$/kg, i.m. had no effect on general behavior in mice, chemo- and electro-convulsions in mice, writhing syndrome induced by acetic acid in mice, the barbital sleeping time in mice, body temperature in rats, charcoal meal propulsion in mice and urine and electrolytes excretion in rats. In anesthetized dogs, CJ-50005(0.25 and 0.75 $\mu\textrm{g}$/kg, i.v) did not alter the respiratory rate, blood pressure, heart rate, femoral blood flow and ECG. In in vitro experiment, CJ-50005 at the concentration up to 0.02 $\mu\textrm{g}$/ml did not produce any changes in the contractions of the isolated ileum of guinea pigs caused by acetylcholine, histamine or $BaCl_2$. Since these pharmacological effects of CJ-50005 were observed at dose much greater than those in clinical use (approximately 0.025 $\mu\textrm{g}$/kg, i.m.), it is likely that this vaccine may be relatively free of undesirable effects in clinical practice.