• BMB Reports
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• v.29 no.1
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• pp.27-31
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• 1996

Using topoisomerase II (topo II) isozyme-specific antibodies, we investigated the phosphorylation of topo $II{\alpha}$ in mitotic HeLa cells. Topo $II{\alpha}$ was specifically modified in the mitotic cells, resulting in slow migration on SDS-polyacrylamide gel electrophoresis. To characterize the nature of this modification, we treated the nuclear extracts prepared from the mitotic cells with alkaline phosphatase. After the treatment with alkaline phosphatase, the slowly migrated band disappeared and instead a normal (170 kDa) topo $II{\alpha}$ band appeared. These results indicate that human topo $II{\alpha}$ is modified at a specific site(s) in M phase by phosphorylation, supporting the possibility that M phase-specific phosphorylation of topo II is critical for mitotic chromosome condensation and segregation.

• The Korean Journal of Zoology
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• v.29 no.4
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• pp.283-293
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• 1986

For the preliminary step to make and characterize the monoclonal antibodies of human alpha-fetoprotein (AFP) was purified from 534g of human fetal tissues through the procedures of tissue extraction, DEAE-cellulose, concanavalin A-Sepharose, Cibacron Blue F3GA-agarose and immunoadsorbent affinity chromatography. The isolated AFP preparation showed a single band on polyacrylamide gel electrophoresis and a single precipitin are against rabbit anti-human cord serum and anti-human AFP on immunoelectrophoresis. Our AFP also displayed a single band on SDS-polyacrylamide gel electrophoresis. The recovery of AFP was 8.76mg total.

• Journal of Yeungnam Medical Science
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• v.10 no.2
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• pp.360-368
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• 1993

GTP binding protein (G-protein) associated with membrane and involved in signal transduction was isolated from bovine brain, and molecular weight of G protein was observed. As the results, cell membranes were homogenized from bovine brain tissues and proteins of membrane were gained using 1% cholate, and progressed the chromatography. The purification process was performed by step, DEAE-Sephacel, Ulttrogel AcA 34 and heptylamine-Sepharose column chromatography. The chromatographic fractions were confirmed by GTP binding assay and SDS-polyacrylamide gel electrophoresis. Molecular weight of $Go{\alpha}$ was revealed 39,000 dalton and $G{\beta}$ 36,000 dalton. One more step of heptylamine-Sepharose was enforced to purify the GTP binding protein. Finally I gained the GTP binding protein isolated subtype of $Go{\alpha}$ and $G{\beta}$.

• Journal of The Korean Astronomical Society
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• v.31 no.2
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• pp.101-104
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• 1998

We have obtained photometry of stars in NGC 2264 with several combinations of H$\alpha$ filters and continuum filters. The main purpose of these observations was to determine the best filter combination for selecting low ma!,s member stars in their Pre-Main Sequence (PMS) stage using H$\alpha$ photometry. A narrow band H$\alpha$ filter (${\Delta}{\lambda}$ = $l0{\AA}$) with any combination of continuum filters showed the highest resolution in the H$\alpha$ photometry.

• Journal of the Korean Society of Safety
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• v.28 no.3
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• pp.107-113
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• 2013

Problem solving and/or decision making process usually encountered in human living consists of a sequence of human behaviors based upon his/her knowledge. Thus, Rasmussen introduced Skill-Rule-Knowledge paradigm to countermeasure human errors that can occur in Nuclear Power Plants. Unfortunately however, it was not so easy as expected since objective evidence have not been obtainable with conventional research techniques. With the help of EEG band pawer ratio techniques, this study tried to get psycho-physiological symptoms of human errors, if any, while human beings perform knowledge-based behaviors such as simple arithmetic computations with different difficulty level. A set of simulated works was carried out with a computer station. Four kinds of arithmetic computation tasks were given to 10 health male under-graduate students on different day individually, and during the experiment, EEG and ECG was measured continuously for objective psycho-physiological analysis. According to the results, ${\alpha}$/(${\alpha}+{\beta}$) as well as ${\alpha}/{\beta}$ band power ratio were sensitive to task difficulty level which consistently decreased both. However, any one of them failed to reveal the influence of tasks with different difficulty level in the aspect of task duration time. On the contrary, Heart Rate Variability was more suggestive than expected. To make a conclusion, it can be said that band power of EEG waves will be helpful in not only assessment of work difficulty level but also assessment of workers' skill development if supported by cardiac function such as HRV.

• Dementia and Neurocognitive Disorders
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• v.21 no.4
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• pp.126-137
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• 2022

Background and Purpose: Early detection of subjective cognitive decline (SCD) due to Alzheimer's disease (AD) is important for clinical research and effective prevention and management. This study examined if quantitative electroencephalography (qEEG) could be used for early detection of AD in SCD. Methods: Participants with SCD from 6 dementia clinics in Korea were enrolled. ¹⁸F-florbetaben brain amyloid positron emission tomography (PET) was conducted for all the participants. qEEG was performed to measure power spectrum and source cortical activity. Results: The present study included 95 participants aged over 65 years, including 26 amyloid PET (+) and 69 amyloid PET (-). In participants with amyloid PET (+), relative power at delta band was higher in frontal (p=0.025), parietal (p=0.005), and occipital (p=0.022) areas even after adjusting for age, sex, and education. Source activities of alpha 1 band were significantly decreased in the bilateral fusiform and inferior temporal areas, whereas those of delta band were increased in the bilateral cuneus, pericalcarine, lingual, lateral occipital, precuneus, posterior cingulate, and isthmus areas. There were increased connections between bilateral precuneus areas but decreased connections between left rostral middle frontal area and bilateral frontal poles at delta band in participants with amyloid PET (+) showed. At alpha 1 band, there were decreased connections between bilateral entorhinal areas after adjusting for covariates. Conclusions: SCD participants with amyloid PET (+) showed increased delta and decreased alpha 1 activity. qEEG is a potential means for predicting amyloid pathology in SCD. Further longitudinal studies are needed to confirm these findings.

• Archives of Pharmacal Research
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• v.26 no.5
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• pp.375-382
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• 2003

Effects of ginsenosides on nitric oxide (NO) production induced by lipopolysaccharide plus TNF-$\alpha$ (LNT) were examined in C6 rat glioma cells. Among several ginsenosides, ginsenoside Rd showed a complete inhibition against LNT-induced NO production. Ginsenoside Rd attenuated LNT-induced increased phosphorylation of ERK. Among several immediate early gene products, only Jun Band Fra-1 protein levels were increased by LNT, and ginsenoside Rd attenuated Jun Band Fra-1 protein levels induced by LNT. Furthermore, LNT increased AP-1 DNA binding activities, which were partially inhibited by ginsenoside Rd. Our results suggest that ginsenoside Rd exerts an inhibitory action against NO production via blocking phosphorylation of ERK, in turn, suppressing immediate early gene products such as Jun Band Fra-1 in C6 glioma cells.

• Journal of the Korean Society of Safety
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• v.26 no.3
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• pp.83-90
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• 2011

It is well known that urgency resulted from time stress can be a great cause to industrial accidents. Therefore, time stress has been studied in the aspect of macroscopic view, namely industrial safety management, but has not been studied in microscopic view such as psychophysiological approach. Among diverse psychophysiological indices, Electroencephalogram(EEG) would be on of the most objective psychophysiological research technique on human errors though few research has been taken yet. This study aimed to get characteristics of human error while committing a simple arithmetic addition task by utilizing the power spectrum technique of EEG data. Each experiment was composed of 2 tasks under different condition - with and without time stress. As subjects, 5 young undergraduate students in their early twenties participated in this study. The results advocated a well-known fact that time stress downgrades the performance of human workers. However, correct answer rate and response time were not significantly influenced by time stress factor which might be explained by the constructural factor adopted in the present study. As in the previous studies, among various EEG-related measures, relative band power ratios of ${\alpha}$ and ${\beta}$ waves to sum of ${\alpha}$,${\beta}$,${\theta}$ wave powers, namely $P_{{\alpha}/({\alpha}+{\beta}+{\theta})}$ and $P_{{\beta}/({\alpha}+{\beta}+{\theta})}$ seemed to be the most effective measures to grasp variation of brain activities in time-stressed situation so that discussions were expanded about their variations.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.28 no.3
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• pp.135-139
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• 2018

In this study, we report the effect of VI/III ratio on ${\alpha}-Ga_2O_3$ epilayer on sapphire substrate by halide vapor phase epitaxy. The surface of ${\alpha}-Ga_2O_3$ epilayer grown with various VI/III ratios was flat and crack-free. To analyze the optical properties of the ${\alpha}-Ga_2O_3$ epilayers, the transmittance and an optical band gap were measured. The optical band gap was shown to be around 5 eV and showed a proportional increase in VI/III ratios. To determine the crystal quality of alpha gallium oxide grown with a ratio of 23, closed to the theoretical optical band gap, the FWHM was measured by HR-XRD. The calculated dislocation density of screw and edge were $1.5{\times}10^7cm^{-2}$ and $5.4{\times}10^9cm^{-2}$, respectively.

• YAKHAK HOEJI
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• v.31 no.6
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• pp.402-404
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• 1987

Fermentation processes have been employed to produce 17-ketosteroids from sterol with chelator of Fe$^{2+}$ such as $\alpha$, ${\alpha}'$-dipyriyl. To analyze the products of sterol fermentation, we developed a simple TLC method without interference of the chelator $\alpha$, ${\alpha}'$-dipyridyl, using Ag$^+$ band on TLC plate.